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Citations for Roche :
551 - 600 of 983 citations for 6 Bromo 3 pyridazinecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: RNA extraction from A549 cells grown in 6-well plates was done using High Pure RNA isolation kit (Roche). Transcriptor First strand kit (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was stored as flow through and the beads were washed 6 times with 1 ml RIPA buffer complemented with 0.25× protease inhibitor (cOmplete, Roche). Bound proteins were eluted from agarose beads with 1 bead volume of Laemmli buffer heated at 95°C for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... αSMA-tk;RFP mice and C57BL/6 mice received intraperitoneal (i.p.) injections with 12.5 mg/kg of body weight of ganciclovir (GCV, Cymevene®, Roche) every 48h ...
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bioRxiv - Cell Biology 2024Quote: ... After washes, the cells were stained with the DNA stain DAPI (4, 6 diamidino-2-phenylindole dihydrochloride) (Roche, #1023627001) and mounded with Prolong gold anti-fade mound media (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... L6 cells and cardiomyocytes by incubating them in their respective media containing 2% (w/v) fatty acid-free bovine serum albumin (FAF-BSA; Roche) and 0.4 mM sodium palmitate (Sigma ...
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bioRxiv - Genetics 2021Quote: ... For whole-genome sequencing DNA was extracted using the MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche Diagnostics GmbH).
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bioRxiv - Genomics 2019Quote: ... The supernatants from the digestion and initial decalcification step were purified using a 5 M guanidine-hydrochloride binding buffer with a High Pure Viral Nucleic Acid Large Volume kit (Roche). The extract was eluted in 100 μl of a 10mM tris-hydrochloride ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.7 µM of total delta-Phe aa-tRNA (total tRNA charged with all amino-acids except Phe; tRNA from Roche) and 100 nM of EF-G were used ...
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bioRxiv - Immunology 2019Quote: ... Samples were washed in 1X maleic acid buffer with 0.1% Tween-20 (MBST) and then incubated in Roche Blocking Reagent (Roche, 1096176) with 10% heat inactivated sheep serum (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Viral transport media was added to each pool at a 1:1 ratio for nucleic acid extraction performed on the Roche MagNA Pure 24 platform using the MagNA Pure 24 Total NA Isolation kit (Roche). Elution volume was set to 50 ul to concentrate viral RNA ...
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bioRxiv - Microbiology 2021Quote: ... was extracted from inactivated samples (200 μL) using the MagNA Pure Compact instrument and MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche Molecular Systems Inc ...
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bioRxiv - Neuroscience 2021Quote: ... medium was replaced with E6 medium (DMEM/F-12 supplemented with 64 mg/L L-ascorbic acid 2-phosphate magnesium, 14 µg/L sodium selenium, 543 mg/L sodium bicarbonate, mg/L insulin [Roche, Penzberg ...
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bioRxiv - Microbiology 2019Quote: ... Sendai Virus (SeV) and Modified vaccinia Ankara (MVA)-gfp viral stocks were extracted using the High Pure Viral Nucleic Acid Kit (Roche). When indicated ...
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bioRxiv - Cell Biology 2020Quote: ... MEFs were seeded subconfluently o/n (unless indicated otherwise) onto coverslips acid-washed and coated with human fibronectin (25 μg/ml, #11051407001, Roche), as described (Dimchev and Rottner ...
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bioRxiv - Neuroscience 2021Quote: ... Two AfeI restriction sites were sequentially added by two rounds of PCR-amplification on each side of individual IDR (see Fig. 1b and S1b for amino acid position) using KAPA HiFi polymerase (Roche). The PCR reaction was DpnI digested (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 200 μl of another aliquot of lung homogenate supernatant using the MagNA Pure Total Nucleic Acid Isolation protocol and reagents on a MagNA Pure LC 2.0 instrument (Roche Diagnostics). Prior to extraction ...
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bioRxiv - Microbiology 2020Quote: ... whole specimens were processed into head/thorax homogenates for RNA extraction with the High Pure Viral Nucleic Acid Kit (Roche), according to manufacturer’s instructions (Moreira et al. ...
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bioRxiv - Biochemistry 2019Quote: ... and S278E amino acid substitutions were introduced into human RACK1 using the QuickChange II XL site-directed mutagenesis kit (Roche). All plasmids are available upon request.
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bioRxiv - Genomics 2019Quote: ... Samples were washed in 1X maleic acid buffer with 0.1% Tween-20 (MBST) and then incubated in Roche Blocking Reagent (Roche, #1096176) with 10% heat inactivated sheep serum (Sigma ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from homogenized tissue of adenovirus vectored vaccine inoculated mice by High Pure Viral Nucleic Acid Kit (Roche). DNA fragments of Sad23L and Ad49L vectors were amplified by nested PCR with primers specific to adenoviral hexon sequences (Table S4 ...
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bioRxiv - Genomics 2022Quote: Cells were lysed in RIPA buffer (Beyotime, China) containing protease inhibitors (Complete Mini Ethylene Diamine Tetraacetic Acid-Free Protease Inhibitor Cocktail, Roche). The protein lysates were centrifuged ...
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bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids were spotted (for dot-blot) or electro transferred (for northern-blot) to positively-charged nylon membranes (Roche Diagnostics), and hybridized with DIG-labeled full-length riboprobes as previously described [8 ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids were spotted (for dot blotting) or electro-transferred (for Northern blot) to positively-charged nylon membranes (Roche Diagnostics), and hybridized with DIG-labeled riboprobes as previously described (22).
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bioRxiv - Microbiology 2023Quote: ... and nucleic acid extracted using the MagNA 96 instrument with DNA/Viral NA small volume kits (Roche, Laval, Quebec, Canada). Ophidiomyces ophidiicola was not detected in these samples by qPCR (Allender et al ...
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bioRxiv - Biochemistry 2023Quote: ... uridine 5’-diphospho-N-acetyl-glucosamine (UDP-GlcNAc) and cytidine-5’-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) were obtained from Roche Diagnostics [UDP-Gal ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were incubated for 1 hour in TBST and then in Blocking Buffer (2% blocking reagent in maleic acid buffer pH 7.5 (Roche, #11096176001) and 10% sheep serum (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked with freshly prepared blocking solution (100 mM maleic acid, 150 mM NaCl, pH 7.5, 1% (wt/vol) blocking reagent (Roche, 11096176001)) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: For Figures 2 and 6: Cells or embryos were lysed in RIPA buffer supplemented with a cocktail of protease inhibitor (Roche). Total protein was resolved on SDS polyacrylamide gels (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: Total RNA from 4 replicates of BCBL-1 WT clone B4 and 57KO clone #6 cells were isolated by TriPure Reagent (Roche) 24 h after induction with VA ...
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bioRxiv - Molecular Biology 2021Quote: T98G cell pellets were fixed by dropwise addition of cold 70% ethanol and their DNA was stained with 4’,6-diamino-2-phenylindole (1 μg/ml, Roche), 0.1% Triton X-100 in phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2020Quote: ... and co-transfected into 60-70% confluent BHK cells grown in 6-well plate tissue culture plates using Fugene HD transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 6-10ng of immunoprecipitated material was used for ChIP-seq library preparation using the KAPA Hyper prep kit (KAPA Biosystems). Libraries were sequenced on an Illumina HiSeq 2500 platform on SE50 mode.
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bioRxiv - Cell Biology 2019Quote: ... the cells became 70-80% confluent and were transfected with the corresponding plasmids using the transfection reagent FuGENE 6 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... along with the packing plasmid psPAX2 (16.2 μg) and envelope plasmid pMD2.G (1.8 μg) were transfected using Fugene 6 (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: HeLa cells were plated in 6-well plates and infected with virus at equal amounts of p24 after treatment with DNaseI (Roche) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Cells were transfected with 250-800 ng DNA per well of a 12-well plate or 500 ng per well of a 6-well plate using X-tremeGENE 9 DNA transfection reagent (Roche), and harvested after 24 h.
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bioRxiv - Biophysics 2022Quote: ... the force was kept at 11 pN and Pif1 was added to the microfluidic chamber at a concentration of 6 nM and 1 mM ATP Lithium salt (Roche) in G4-Pif1 buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Glucose content was then measured by spectrophotometry after a 2 enzymes reaction with hexokinase and glucose-6-phosphate dehydrogenase (Roche).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with RCAS-hPDGFB-HA and RCAS-shRNA-p53-Rfp using a Fugene 6 transfection kit (Roche, 11814443001) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... COS-7 cells were plated in 6-well plates (100,000 cells/well) and transfected the next day using X-tremeGENE™ 9 DNA (Transfection Reagent, Roche), with HA-NLGN1 + AP-MDGA1 or AP-MDGA2 + BirAER (1 μg/well) ...
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bioRxiv - Neuroscience 2019Quote: DIV14-15 cortical neurons (250.000 neurons/mL in laminin/poly-L-ornithine coated 6-well plates) were washed and scraped with ice cold PBS with protease inhibitor cocktail (Roche). Neurons were pelleted (5 minutes at 3000 g at 4°C) ...
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bioRxiv - Neuroscience 2021Quote: ... were either left empty or filled with 6 μl of a mix of post-translational modification (PTM) inhibitors containing the following reagents: phosphatase inhibitors (PhosSTOP, 04906845001, Roche), protease inhibitors (cOmplete Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2022Quote: ... 500-1000 ng of sheared DNA was used as a template for a 6-cycle PCR to construct a fragmented library using the KAPA HTP Library Preparation Kit (Roche). Exome enrichment was performed using SeqCap EZ Enrichment Kit (Roche ...
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bioRxiv - Genomics 2022Quote: ... The eluted material and input were incubated for 6 h at 65°C to reverse crosslinks and were treated with 100 ng RNaseA (Roche) for 1 h at 50°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Embryos were then washed with wash buffer and their nuclei stained with 5 ug/mL DAPI (4′,6-diamidino-2-phenylindole, Roche) prepared in wash buffer for 10 minutes at 30 ºC.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were transfected with 1 μg Kras or control expression plasmid and 1 μg of viral packaging plasmids (250 ng pMD2.G and 750 ng psPAX2) using 6 μl of X-tremeGENE 9 transfection reagent (Roche). After 24 hours of transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were transfected with 1 ug of shRNA/cDNA and 1 ug of viral packaging plasmids (250ng pMD2.G and 750ng psPAX2) using 6 ul of Xtreme Gene 9 transfection reagent (Roche). After 24 hr ...
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bioRxiv - Cell Biology 2019Quote: Lentivirus was produced using 293T cells (a gift from G. Green, University of Chicago, Chicago, IL) using Fugene 6 transfection reagent (Roche) to transfect the lentiviral vectors ...
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bioRxiv - Genetics 2021Quote: ... each sample was amplified in 6/2 PCR reactions (2 µg DNA/reaction) in the primary/secondary screens using the ReadyMix Kapa polymerase (Roche) with a total of 20 cycles and an annealing temperature of 55°C (Primer sequences in Table S4 ...