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Citations for Roche :
1 - 50 of 6988 citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... was incubated with 50μl MTT (sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate) labeling reagent (Roche Life Science, USA) to each well ...
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bioRxiv - Genetics 2023Quote: ... with 6-8 cycles of PCR amplification using KAPA-HiFi (Kapa Biosystems, KK2602). DNA library was sequenced using an Illumina NextSeq 500 75-cycle high output kit.
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bioRxiv - Cell Biology 2020Quote: ... lower hind limb muscles from C57BL/6 mice aged 6-8 weeks were dissected and digested with collagenase (Roche). Isolated cells were plated on matrigel-coated dishes and allowed to grow in DMEM containing 20% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2020Quote: ... Cells pellets were combined and lysed in a denaturing urea buffer (8 M urea, 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml ...
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bioRxiv - Microbiology 2022Quote: ... Beads were then washed three times with 400 μL wash buffer (25 mM Tris-HCl pH 8, 150 mM NaCl, 1 mM EDTA pH 8, cOmplete protease inhibitor cocktail (Roche), 5% glycerol) ...
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bioRxiv - Plant Biology 2021Quote: ... in paraffin-embedded sections (8µm) and color was detected with 5-bromo-4-chloroindol-3-yl phosphate/nitrateblue tetrazolium (BCIP/NBT) (Roche).
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 10 mL of L2 buffer (200mM NaCl 1mM EDTA pH 8 0.5mM EGTA 10mM Tris, pH 8, dH2O, 1 protease inhibitor tablet (Roche Complete cat #1 697 498) per 50ml buffer ...
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bioRxiv - Cell Biology 2023Quote: MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Roche) was performed following manufacturer’s indications ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 200 mL of cold Lysis buffer (50 mM HEPES pH 8, 300 mM NaCl, 1 mM EDTA, 5 % glycerol, 4 tablets of protease inhibitors, Roche). The cells were lysed and the membrane fraction was collected as described in the YukC purification protocol below ...
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bioRxiv - Biochemistry 2022Quote: ... was from Roche (9048-46-8). Poly-D-lysine and LiCl were from Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... 8 mg/mL collagenase P (Roche) was added to the trypsin mixture for thorough digestion ...
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bioRxiv - Microbiology 2023Quote: ... 1ml of CDP-Star® Chemiluminescent Substrate (Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.13.7]decan])-4-yl]-1-phenyl phosphate) (Roche, Cat No. 11685627001) was added to 9ml of DIG-detection buffer and membranes were then incubated with the substrate for 5 min ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
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bioRxiv - Neuroscience 2021Quote: ... pH 8) containing phosphatase (PhosSTOP tablets, Roche) and protease inhibitor (Complete Tablets ...
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bioRxiv - Biochemistry 2021Quote: ... pH 8 and complete protease inhibitor (Roche) were then added to the cells suspension and incubation continued 30 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mM EDTA pH 8) in presence of Complete Protease Inhibitor Cocktail (Roche) and Halt Phosphatase Inhibitor Cocktail (Pierce) ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Molecular Biology 2020Quote: ... and then deproteinized by adding of 8 µl of 10% SDS and 8 µl of proteinase K (19 mg/ml stock, Roche). Deproteinization was carried out at 37 °C for 30 min with gentle flicking ...
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bioRxiv - Molecular Biology 2019Quote: ... ~2 x 108 parasites were resuspended in 2 mL cytoplasmic lysis buffer (10 mM HEPES [pH 8], 10 mM KCl, 0.1 mM EDTA [pH 8], plus complete protease inhibitor [PI, Roche # 11836170001]), transferred to a prechilled 2 mL douncer homogenizer and set on ice for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... grown for 24 hours and transfected with 8 μg of plasmids and 8 μl of X-tremeGENE HP DNA Transfection Reagent (Roche). The cells were grown for 48 hours post-transfection and collected for downstream analyses.
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bioRxiv - Biochemistry 2021Quote: ... 8 M urea and protease inhibitor (Roche, 05892791001) with sonication on ice ...
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bioRxiv - Neuroscience 2020Quote: ... at a ratio of 4.5:4.5:1 (GluN1/GluN2A/EGFP) using X-treme GENE HP (Roche) (for details on cell culture and transfection see Amin et al. ...
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bioRxiv - Plant Biology 2023Quote: ... before to add chemiluminescent substrate CSPD® (disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2’-(5’-chloro)tricyclo [3.3.1.13,7]decan}-4-yl) phenyl phosphate) (Roche). The chemio-luminescent signal was detected by using a G-Box (Syngene).
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bioRxiv - Neuroscience 2023Quote: ... tissue sections (3-5μm) from frontal cortex (BA 8/9) using the Nexes station automated system (Ventana Medical System Inc., Roche). Deparaffinised and rehydrated sections were pretreated and blocked ...
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bioRxiv - Cancer Biology 2023Quote: ... for 8 min followed by post-coloration using Bluing reagent for 4 min at RT (Roche Diagnostic, Meylan, France). The slides were then dehydrated (ethanol and xylene ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Developmental Biology 2023Quote: ... with 8% PFA in PBS supplemented with phosSTOP (Roche) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... pH 8) containing 1 mM PMSF and Complete™ EDTA free protease inhibitor cocktail (Roche). Nuclei were removed by centrifugation for 1 min at 6,000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... and 20 mM Tris pH 8) supplemented with 1 mg/mL of protease inhibitors (Roche). Cell lysates were sonicated for 10 cycles with Bioruptor Pico (Diagenode ...
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bioRxiv - Plant Biology 2019Quote: ... The fine powder was resuspended with the extraction buffer (50 mm Tris, pH 8, 8 M urea, and 0.5% SDS) supplemented with protease inhibitor (Roche Diagnostics GmbH, Mannheim, Germany), and homogenized with a Dounce homogenizer ...
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bioRxiv - Biochemistry 2022Quote: ... pellets were resuspended in lysis buffer (50 mM HEPES pH 8, 400 mM NaCl, 75 mM Imidazole pH 8, 5% v/v glycerol, 5 mM 2-Mercaptoethanol, supplemented with Roche Protease Inhibitor Tablets) and lysed using an Emulsiflex-05 homogenizer ...
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bioRxiv - Biochemistry 2021Quote: ... Then qPCR was performed in triplicate using 4 μl sample in 8-9 μl reaction with FastStart Universal SYBR Green Master Mix (Roche) and primers flanking each MseI cassette (Table S3).
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatants were incubated for 16 h while rotating at 4°C with 8 µg/ml anti-GFP antibody (Roche). Protein A/G PLUS-Agarose (Santa Cruz ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with ice-cold PBS two times and lysed in 300 μl RIPA buffer (50 mM TRIS pH 8, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, Roche Complete protease inhibitors ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with ice-cold PBS two times and lysed in 300 μl RIPA buffer (50 mM TRIS pH 8, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, Roche Complete protease inhibitors ...
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bioRxiv - Microbiology 2020Quote: ... were amplified using primers Sb.1 - Sb.2 and Sb.3 to Sb.8 (Table 2) and labeled with digoxigenin (DIG) using the PCR DIG probe synthesis kit (Roche Diagnostics). Membranes were hybridized with these probes at 65°C and then washed at 68°C with decreasing concentrations (from 2X to 0.2X ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 10 mM Tris pH 8, 3 mM MgCl2, 0.5% NP-40, 0.15 mM spermine, 0.5 mM spermidine, Roche EDTA-free protease inhibitor) and incubated on ice for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM Imidazole pH 8) and supplemented with PMSF (1:100), protease-inhibitor mix HP Plus (1:500, Serva) and DNaseI (1:300, Roche), lysed by sonication and cleared by centrifugation at 108000g for 45 min ...
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bioRxiv - Microbiology 2019Quote: ... 8 mM EDTA) with Complete™ protease inhibitor cocktail (Roche). The Triton X-100-insoluble material was pelleted twice by centrifugation (10 min at 16,000 g) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and phenol (phenol-Tris saturated, pH 8; Roche, Indianapolis, IN) and chloroform:isoamyl alcohol (American Bioanalytical ...
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bioRxiv - Biochemistry 2022Quote: ... pH=8) supplemented with EDTA-free protease inhibitor mixture (Roche) and 100 mM PMSF ...
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bioRxiv - Microbiology 2023Quote: ... and suspended in 8 M urea containing PhosSTOP (Roche Diagnostics) and Benzonase (Novagen) ...
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bioRxiv - Biophysics 2023Quote: Microchambers were incubated with 8 mg/µl anti-digoxigenin (Roche) at 25°C for 90 min and then the blocking buffer (PBS with 1% α-casein ...
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bioRxiv - Cancer Biology 2019Quote: ... 3 and 6 post transfection using WST-1 dye (Roche, Basel, Switzerland) as previously described 17.
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bioRxiv - Microbiology 2020Quote: ... pH 8) supplemented with protease inhibitors (1 tablet of complete protease inhibitor per 20 mL; Roche, Mannheim ...
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bioRxiv - Biophysics 2022Quote: ... Frozen bacteria containing full-length or ΔUBLΔUBA UBQLN constructs were resuspended in Buffer A (50 mM Tris pH 8, 1 mM MgCl2, 1 mM PMSF, and 0.2 mg/mL DNase I and Roche Complete Mini protease inhibitor cocktail) ...
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bioRxiv - Genomics 2022Quote: ... before being re-pelleted by centrifuging for 3 minutes at 4°C at 500g and resuspended in 180µl TMS buffer (10mM Tris-HCl pH 8, 1mM MgCl2, 1% SDS, 1× Complete Protease Inhibitor Cocktail (PIC, Roche)) with 0.4µl benzonase (E1014 ...
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bioRxiv - Systems Biology 2022Quote: ... 6 mM MgCl2, 1 mM EDTA, 100 mM NaCl, 0.1% Tx-100, pH 8, and 1× Protease Inhibitor Cocktail, Roche) at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Nematodes were resuspended in 8 M urea in 100 mM TRIS-HCl buffer (pH 8) containing cOmplete™ protease inhibitor cocktails (Roche Diagnostic Canada, Laval, QC, Canada) and aliquoted into reinforced 1.5 mL homogenizer tubes containing 500 μm glass bead homogenizer tubes with 25 mg of glass beads ...