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Citations for Roche :
1 - 50 of 3683 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 12.5 mM EDTA) containing 100 μl proteinase K (recombinant PCR grade solution, Roche) was added to each sample and digested at 65 °C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... 400 μl sonication solution and 3.8 μg/μl of proteinase K (proK, Recombinant PCR Grade [Roche]) for fraction B2 ...
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bioRxiv - Immunology 2021Quote: Liberase Thermolysin Medium Research Grade solution (Roche) was used for the digestion of IE9mp1-mIDO and IE9mp1-EV tumor tissues harvested from WT C57BL/6 and IDOKO mice ...
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bioRxiv - Plant Biology 2021Quote: ... 4 μl of PCR-grade water (Roche), 1 μl of the corresponding primer pair (10 μM each ...
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bioRxiv - Neuroscience 2021Quote: ... PCR-grade Proteinase K (Roche, Cat#03115828001) was added and incubated at 45°C and 650 rpm for another hour ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL trypsin solution (100 ng μL−1 Sequencing grade, Roche Deutschland Holding GmbH ...
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bioRxiv - Microbiology 2020Quote: ... and final elution in 40 µL PCR-grade water (Roche). Extraction blanks were processed alongside to assess laboratory contamination ...
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bioRxiv - Microbiology 2020Quote: ... and final elution in 40 μL PCR-grade water (Roche). Extraction blanks were processed alongside to assess laboratory contamination ...
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bioRxiv - Genetics 2022Quote: ... 30 μl proteinase K PCR-grade (20 mg/ml) (Roche) and 150 μl Sarkosyl 20% (Sigma) ...
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bioRxiv - Physiology 2023Quote: ... and PCR-grade proteinase K (Roche #RPROTK-RO, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and Proteinase K (194 μg/ml, recombinant, PCR grade, Roche) for 2 hours at 56 °C to achieve cell lysis and protein digestion ...
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bioRxiv - Evolutionary Biology 2022Quote: 2 μl 1x proteinase K (recombinant PCR Grade, 25 mg - Roche) dissolved in 2,5 ml TE (10 mg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were cooled to 55°C and PCR grade Proteinase K (Roche) was added to a concentration of 1 ug/ul ...
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bioRxiv - Microbiology 2020Quote: ... bead-captured RNAs were digested with 100 ng Proteinase K (PCR grade, Roche) and incubated at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Cytosolic ribosome fractions were collected and digested with PCR grade proteinase K (Roche) in the presence of 1% SDS prior to RNA isolation using TRIzol (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1M Resolution Solution (GC-RICH PCR, Roche) was added to each qPCR reaction (47) ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µL PCR grade water and 12.5 µL KAPA HiFi HotStart ReadyMix (Roche, Indianapolis, USA). Samples were normalized and pooled in equimolar amounts and the pools were sequenced on the Illumina MiSeq machine with the 2 x 300 bp V3 kit at the USEQ sequencing facility (Utrecht University ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µL PCR grade water and 12.5 µL KAPA HiFi HotStart ReadyMix (Roche, Indianapolis, USA). The PCR conditions were 98°C for 5 min ...
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bioRxiv - Physiology 2023Quote: ... the heart was perfused with Ca2+-free Tyrode’s solution containing LiberaseTM research grade (Roche Diagnostics, Basel, Switzerland) for 10 min at 37°C for enzymatic dissociation ...
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bioRxiv - Microbiology 2021Quote: ... 10μL PCR grade water and 25 μL of KAPA© HiFi Hot Start ReadyMix (Roche, Switzerland). The following PCR cycling was used ...
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bioRxiv - Immunology 2023Quote: ... and fractions 7–10 (cytosolic ribosomes) were pooled and treated with PCR grade proteinase K (Roche) in 1% SDS to release ribosome protected fragments ...
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bioRxiv - Cancer Biology 2020Quote: ... grade 2 (Roche), homogenizing for 20min with a plastic Pasteur pipette ...
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bioRxiv - Developmental Biology 2022Quote: ... fins were incubated for 20 min at 28°C with vigorous shaking in a solution of Liberase DH Research Grade (0,05mg/ml in 1x PBS, Roche). Cell suspensions were passed through a 30 μm filter (CellTricks ...
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bioRxiv - Neuroscience 2020Quote: ... Peels were cut in 2-5 mm2 pieces and placed in 5 ml digestion solution (0.75 mg/ml Liberase TH Research grade (Roche), 0.1 mg/ml DNAseI (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... the small intestines were cut into 2-5mm2 pieces and put into digestion solution (0.75mg/ml Liberase TH Research Grade (Roche), 12 U/ml Dispase ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were injected in multiple sites with a total volume of 1.5 mL Liberase enzyme digestion solution (50 μg/mL Liberase DL research grade (Roche) and 10 U/mL DNase I (Sigma ...
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bioRxiv - Physiology 2023Quote: ... enzymatic digestion was carried out for 20-25 min at 36 °C in the low-Ca2+ solution containing Liberase TH Research Grade (0.15 mg/mL; Roche) and elastase (0.5 mg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... sequencing grade enzyme (Roche) per sample and tryptic peptides were desalted using C18 Sep-Pack Cartridges (Waters) ...
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bioRxiv - Biochemistry 2024Quote: Chymotrypsin (Sequencing grade, Roche) was then added in a ratio of 1 µg protease ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA was then purified, treated with RNase H (ThermoFischer, 18021071) and Proteinase K (PCR grade, Roche, 3115836001) and concentrated with the DNA Clean & Concentrator-5 kit (Zymo ...
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bioRxiv - Cell Biology 2024Quote: ... The beads were washed four times with binding and washing buffer and treated with DNAse (PCR grade, Roche) for 10 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Tissue fragments were dissected with sterile scissors in a FBS-free DMEM solution containing 1x Liberase TM Research Grade (Roche) and Antibiotic-Antimycotic (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: ... and washed with 1 mL 1X Wash Buffer (1 mL HEPES pH 7.5, 1.5 m 5M NaCl, 12.5 uL 2M spermidine and 47.5 mL dH2O with 1 Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Neuroscience 2022Quote: ... or 1 mL CUT&RUN 1X wash buffer (1 mL HEPES pH 7.5, 1.5 m 5M NaCl, 12.5 uL 2M spermidine and 47.5 mL dH2O with 1 Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Biochemistry 2024Quote: ... and AspN (Sequencing Grade, Roche) separately ...
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bioRxiv - Microbiology 2023Quote: ... and Sequencing Grade Chymotrypsin (Roche) at an enzyme-to-substrate weight ratio of 1:25 for 3 hours and then overnight at 37 °C in 100 mM phosphate buffer pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... DNAse I (Grade I, Roche)) ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting nuclei were resuspended in Wash Buffer (1 mL 1M HEPES pH 7.5, 1.5 mL 5M NaCl, 12.5 µL 2M spermidine, 1 Roche c0mplete Protease Inhibitor EDTA-free tablet with ddH2O to 50 mL ...
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bioRxiv - Genetics 2024Quote: ... and resuspended in 100 µL Wash Buffer (1 mL 1M HEPES pH 7.5 [ThermoFisher, J60712.AP], 1.5 mL 5M NaCl, 12.5 µL 2M spermidine, 1 Roche c0mplete Protease Inhibitor EDTA-free tablet with ddH2O to 50 mL) ...
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bioRxiv - Immunology 2021Quote: ... and grade II DNAse I (Roche) for 20 minutes with shaking ...
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bioRxiv - Cell Biology 2021Quote: ... a Liberase (TL Research grade, Roche) solution was prepared in un-supplemented RPMI-1640 (Gibco ...
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bioRxiv - Immunology 2020Quote: ... and deoxyribonuclease I (grade II, Roche). Bacteria were lysed by three rounds of sonication (Branson Sonifier 450 ...
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bioRxiv - Immunology 2020Quote: ... and deoxyribonuclease I (grade II, Roche). Extracts were then prepared for in vitro and in vivo proliferation as described above ...
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bioRxiv - Immunology 2020Quote: ... Chymotrypsin (sequencing grade, Roche Applied Sciences) was reconstituted to 1 μg/μL in assay buffer and 5 μL of reconstituted enzyme was added to 80 μL of sample ...
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bioRxiv - Developmental Biology 2023Quote: Dissected embryonic brain structures were lysed in RIPA buffer (Tris HCl 1M, pH 7.7; NaCl 5M; EDTA 0.5M; Triton X-100) supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Sigma Aldrich ...
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bioRxiv - Genetics 2024Quote: ... in 300-Wash Buffer (1 mL 1M HEPES pH 7.5, 3 mL 5M NaCl, 12.5 µL 2M spermidine, and 1 Roche c0mplete Protease Inhibitor EDTA-free tablet with ddH2O to 50 mL ...
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bioRxiv - Cell Biology 2020Quote: ... The solution was refreshed one time before the addition of Liberase TM research grade (final concentration 0.038 mg/mL (Roche Diagnostics, GmbH, Mannheim, Germany)) and Elastase from porcine pancreas (final concentration 0.01 mg/mL (Bio-Connect B.V. ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing grade endoproteinase Lys-C (Roche Diagnostics) and modified trypsin (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... liberase TL Research Grade low Thermolysin (Roche), DNase I ...
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bioRxiv - Cell Biology 2023Quote: ... liberase TL Research Grade low Thermolysin (Roche), DNase I ...