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Citations for Roche :
251 - 300 of 871 citations for 5α CHOLESTAN 3 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and one tablet of Complete mini EDTA-free protease inhibitor cocktail (Roche; 10 ml, pH 7.4) with up to seven strokes at 700 rpm in a Teflon glass homogenizer ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 mM DTT) supplemented with one tablet cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) and 1 mg/ml lysozyme ...
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bioRxiv - Developmental Biology 2022Quote: ... fixed in 2% PFA diluted in HM supplemented with PhosSTOP (04906837001 Roche, one tablet/10 ml) for 15 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... One set specific to the GFP region includes the Universal probe library (UPL) probe #67 (Roche), the forward primer ...
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bioRxiv - Plant Biology 2023Quote: ... RNA extracts were tested by RT-PCR using the Titan One Tube RT-PCR kit (Roche) and the primers of Li et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... with the second one containing 1mM levamisole and then developed colour using BM purple (Roche #11442074001) at 37°C ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times with 1 ml buffer A (15 mM Tris [pH 7.5], 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, one tablet Roche complete EDTA-free mini protease inhibitors ...
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bioRxiv - Biophysics 2024Quote: ... 5% glycerol) per 1 L of cells with one EDTA-free protease inhibitor cocktail tablet (Roche). Cells were lysed using a Misonix Sonicator 3000 (110 W for 2 min total ON-time ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was purified from one million cells with the High Pure RNA Isolation kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... using the gRNA_enrichment1_fw (5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTTGTG-GAAAGGACGAAACACCG-3’) and gRNA_enrichment1_rv (5’-CTACACGAC-GCTCTTCCGATCT-3’) primers and the 2x KAPA HiFi HotStart Ready Mix (Roche, Cat. No. KK2601). In a subsequent PCR ...
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bioRxiv - Microbiology 2022Quote: The V4 hypervariable region of the 16S rRNA gene was amplified with the primer pair 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3) on a Fast Start High Fidelity PCR System (Roche, PQ) using the following conditions ...
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bioRxiv - Neuroscience 2024Quote: ... 5’-ATGGGCACCCAAAACAACAGT-3’ and 5’-GCGGCAGCACATATCCAAAAA-3’) was PCR amplified with a fast-cycling polymerase (KAPA2G Fast HotStart PCR kit, KAPA Biosystems) and a subsequent gel-electrophoresis ...
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bioRxiv - Cancer Biology 2020Quote: ... An overnight blunt ligation was induced for the 5’ of exon 1 and the 3’ of exon 3 (5 U T4 DNA ligase (Roche, Basel, Switzerland)) at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Cell Biology 2021Quote: ... and 3× EDTA-free complete protease inhibitor cocktail (Roche). Lysates were briefly sonicated until the protein solution was clear ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with blocking buffer containing 3 % BSA (Roche Diagnostics), 5 % goat serum (Jackson ImmunoResearch Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: Clonazepam (CAS #1622-61-3) was bought from Roche on 2018 ...
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bioRxiv - Developmental Biology 2023Quote: ... 5-Bromo-4-chloro- 3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Biochemistry 2023Quote: ... 3 cOmplete EDTA-free protease inhibitor Cocktail tablets (Roche), pH 7.2 ...
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bioRxiv - Developmental Biology 2024Quote: ... 5-bromo-4-chloro-3-indolyl-phosphate (BCIP, Roche), and levamisole (1359302 ...
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bioRxiv - Biophysics 2024Quote: ... 20 mM imidazole) supplemented with 3 protease inhibitors (Roche) and then lysed by an EmulsiFlex-C3 (Avestin ...
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bioRxiv - Cancer Biology 2021Quote: ... First-strand cDNA synthesis and RT-qPCR was carried out using One step RT QPCR reagent (Roche). Resulting signal was detected on Applied Biosystems Real Time PCR System ...
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bioRxiv - Synthetic Biology 2019Quote: ... previously supplemented with one tablet (per 10 ml of buffer volume) of complete protease inhibitor cocktail (Roche). Cell suspensions were incubated on ice for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... pH 8.0) containing 1 mg/mL lysozyme and one complete mini EDTA-free protease inhibitor pellet (Roche). Cell suspensions were incubated on ice for 20 min before sonication ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M NaCl 1 mM TCEP) supplemented by one protease inhibitor cocktail tablet Complete EDTA-free (Roche) and centrifuged for 30 min at 18,000 g 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and resuspended in one pellet volume of lysis buffer with complete EDTA-free protease inhibitor cocktail (Roche) before purification ...
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bioRxiv - Microbiology 2020Quote: ... One step real time RT-PCR was performed using the RNA Process Control Kit (Roche, Basel, Switzerland) with 5 μl of extracted RNA or directly from 5 μl of heated sample ...
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bioRxiv - Immunology 2022Quote: ... One microgram of total brain RNA was converted to cDNA (Transcriptor High Fidelity cDNA Synthesis Kit; Roche). A final volume of 20 μl of the cDNA reaction was diluted 1:3 in RNase-free water to be used in qRT-PCR ...
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bioRxiv - Genomics 2022Quote: One µg of DNA was used for whole genome bisulfite sequencing library preparation (KAPA Biosystems, Wilmington, MA), bisulphite conversion (Epitect Fast DNA Bisulfite Kit (Qiagen)) ...
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bioRxiv - Physiology 2019Quote: ... supplemented with protease inhibitor cocktail and one PhosSTOP tablet per 10ml (Cat.# 04 906 837 01, Roche) on ice ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription with ensuing PCR steps was performed using a Transcriptor One-Step RT-PCR kit (Roche) with EBOV-specific primers ...
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bioRxiv - Physiology 2021Quote: ... One µg of total RNA was then retrotranscribed using the Transcriptor First Strand cDNA Synthesis kit (Roche) as previously described [15] ...
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bioRxiv - Pathology 2021Quote: Real-time quantitative PCR was performed using KAPA PROBE FAST Universal One-Step qRT-PCR (Roche, Switzerland) and a specific pair of primers and a probe for amplification and detection of ToBRFV (Table S1) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... One thousand ng of total RNA was used for retro-transcription (RT) (Expand™ Reverse Transcriptase, Roche). In the case of mixed infections ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500 ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.5) was prepared with one tablet of protein inhibitor cocktail (cOMPLETE Mini lacking EDTA, 11873580001, Roche) along with 15uL of RNAsin (N2615 ...
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bioRxiv - Cancer Biology 2021Quote: ... counted and washed twice with Wash Buffer (20mM HEPES pH 7.5, 150 mM NaCl, 0.5mM Spermidine and one Roche Complete EDTA free protein inhibitor tablet per 50 mL) ...
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bioRxiv - Cell Biology 2020Quote: ... Lysis buffer which consisted in one tablet of complete mini-EDTA free protease inhibitor cocktail (Roche, Basel, Switzerland) and one tablet of phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Biophysics 2022Quote: ... 300 mM NaCl and 10 mM imidazole pH 8.0) containing one tablet of cOmplete protease inhibitor cocktail (Roche), and lysed by sonication ...
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bioRxiv - Immunology 2019Quote: ... RT-qPCR was performed in triplicates using the one-step system Lightcycler 480 RNA Master Hydrolysis Probes (Roche), with the following PCR profile ...
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bioRxiv - Molecular Biology 2019Quote: ... and permeabilized in wash buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine and one Roche Complete protein inhibitor tablet per 50 mL ...
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bioRxiv - Biophysics 2021Quote: ... DNA with digoxigenin at one end was attached to the anti-digoxigenin (Roche Life Science, Indianapolis, IN, USA) coated-coverslips ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram of RNA was reverse transcribed using ‘Transcriptor High Fidelity cDNA Synthesis Kit’ (Roche Diagnostics, Mannheim, Germany) with oligodT as a primer ...
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bioRxiv - Plant Biology 2021Quote: ... and 0.33 M D-sorbitol supplemented with one tablet (per 50 mL) of cOmplete protease inhibitor cocktail (Roche)] using a Waring blender ...
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bioRxiv - Microbiology 2021Quote: ... cells were harvested and resuspended in PBS supplemented with one complete EDTA-free Protease Inhibitor Cocktail tablet (Roche) per 50 mL of lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... patients with at least one nasopharyngeal swab positive for SARS-CoV-2 as determined by qRT-PCR (Roche LightCycler480 ...
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bioRxiv - Genetics 2020Quote: ... and suspended in 1 mL of ice-cold protein extraction buffer (50mM HEPES pH 7.5, 150mM NaCl, 0.02% NP-40, 1mM EDTA, 1mM phenylmethylsulfonyl fluoride [PMSF; Sigma, P7626], one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail [Roche ...
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bioRxiv - Genetics 2020Quote: ... then resuspended in 600 µl of ChIP lysis buffer (50mM HEPES, pH 7.5, 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... and the other one was treated with Lambda PP in the presence of phosphatase inhibitor cocktail (Roche, #04906837001). After incubated at 30°C for 30mins ...
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bioRxiv - Genomics 2021Quote: ... and one cDNA reaction product was split into two for PCR amplification using KAPA HiFi master mix (Roche) together with USER enzyme (NEB ...