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Citations for Roche :
1 - 50 of 2703 citations for 2S 4S 4 Fmoc amino pyrrolidine 2 carboxylic acid methylester hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 2 mM amino acids (Roche), 1 nM pY71sfGFP plasmid encoding superfolder GFP (M ...
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bioRxiv - Biophysics 2022Quote: ... except that the carboxylic silica beads were replaced by polystyrene carboxylic beads and polyclonal anti-digoxigenin from sheep (11333089001, ROCHE) were used in our experiments.
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM Tris-HCL pH 6.8 and 1x ethylenediaminetetraacetic acid (EDTA) free protease inhibitor (Roche) followed by incubation at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: Prior to quantitative amino acid analysis Cel1cat was de-glycosylated using EndoH (Roche Diagnostics GmbH). The de-glycosylation was carried out in 20 mM MES ...
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bioRxiv - Systems Biology 2021Quote: ... After neutralizing the samples with HCl the free fatty acids were assayed using a commercial kit (Roche). Concentrations of cholesterol and acetate were measured using commercial kits supplied by Boehringer Mannheim.
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bioRxiv - Evolutionary Biology 2024Quote: ... was diluted 10-fold with ChIP incubation buffer (70 mM NaCl, 10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 2 mM EDTA, 0.1% Triton, 1 x Roche Complete EDTA-free Protease inhibitor cocktail) ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl extraction buffer (Tris-HCl 50 mM pH 6.8, SDS 2%, DTT 2 mM and 1× protease inhibitors (Roche) and centrifuged for 5 min at 13,000g at 4OC ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed in 100μL or 200μl of 2% SDS lysis buffer (2% SDS, 50mM Tris-HCl pH 7.5, 5mM EDTA, 15U/mL DnaseI (Roche), cOmplete mini EDTA-free protease inhibitor tablet (Roche ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4°) in lysis buffer (50 Tris-HCl, 150 mM NaCl, Roche complete EDTA-free protease inhibitor); centrifugation for 30 min at 4° and 21,000xg allowed isolation of the soluble fraction supernatant.
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bioRxiv - Cancer Biology 2024Quote: ... 4 tetrazolium]-bis(4-methoxy-6-nitro)-benzene sulfonic acid hydrate (XTT)-based colorimetric assay (Roche) according to manufacturer-recommended protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended at 10 mg/mL in AC/BT buffer (1.5M aminocaproic acid and 75mM Bis-Tris/HCl, pH 7.0, supplemented with Complete Mini Protease Inhibitor [Roche Diagnostics, Stockholm, Sweden]), and kept frozen at -80°C until analysis ...
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bioRxiv - Neuroscience 2021Quote: ... Two AfeI restriction sites were sequentially added by two rounds of PCR-amplification on each side of individual IDR (see Fig. 1b and S1b for amino acid position) using KAPA HiFi polymerase (Roche). The PCR reaction was DpnI digested (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.05M Tris HCl pH 7.9, 5μM MgCl2, 5μM CaCl2, 2% SDS supplemented with 1x protease inhibitor cocktail, ROCHE) by sonication on ice ...
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bioRxiv - Cancer Biology 2021Quote: Pellets of 1x107 cells were lysed with 1mL Co-IP Lysis Buffer (300mM NaCl, 50mM Tris HCL pH7.4, 0.5% NP40, 0.1% Sodium deoxycholate, 2% SDS) with PhosSTOP (Roche) and cOmplete (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and library preparation was carried out using the Accel-NGS 2S Plus DNA Library Kit (Swift Bioscience) and amplified with the KAPA HiFi DNA polymerase (Roche). The amplified library was sequenced on NovaSeq 6000 (Illumina ...
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bioRxiv - Biochemistry 2021Quote: ... the amino acid Thr at position 206 was mutated to Trp using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems, USA) together with the designed complementary primers (5’- CCTATCTGATTCATGAGCACATGGTTATTTGGGATCGCATTGAAAAC-3’ and 5’- GTTTTCAATGCGATCCCAAATAACCATGTGCTCATGAATCAGATAGG-3’ ...
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bioRxiv - Systems Biology 2021Quote: ... 2% fatty acid-free bovine serum albumin (BSA) fraction V (Roche), 50 μmol/L 2-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Genomics 2021Quote: ... These cells were re-suspended in lysis buffer (4% SDS, 50 mM Tris-HCl [pH 8.0], 10 mM EDTA, 100 mM NaCl) with Protease inhibitor (Roche) and RNase inhibitor ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in cold extraction buffer (50 mM Tris-HCl pH 7.5 containing 1 mM EDTA, 4 mM DTT, cOmplete Protease Inhibitor Cocktail (Roche), and 1 mM PSMF ...
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bioRxiv - Plant Biology 2024Quote: ... The oocytes were homogenized in 2 ml Lysis buffer (Tris-HCl pH 8.0 50 mM, ½ tablet PhosSTOP (Roche), 1x cOmpleteTM EDTA-free proteinase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Genomics 2024Quote: ... Cells were re-suspended in digestion buffer (50 mM Tris-HCl pH7.6, 2 mM CaCl2, 0.2% NP40) containing protease inhibitors (complete EDTA-free, Roche) and warm-up to 37°C for 2min ...
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bioRxiv - Molecular Biology 2024Quote: ... then Lysis Buffer 2 (10 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x Roche cOmplete™ ...
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bioRxiv - Developmental Biology 2024Quote: ... were added to freshly prepared DB1 buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.5 mM spermidine, 2% glycerol, 1× EDTA-free with Roche complete protease inhibitor ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated chromatin was then indexed with the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences 21024) and amplified with KAPA Library Amplification Kit (Roche KK2620), before paired-end next generation sequencing.
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bioRxiv - Microbiology 2022Quote: ... Cells were washed once with PBS and resuspend with Tris-HCl containing 4 mM EDTA and 1× complete protease inhibitor cocktail (Roche). Dounce homogenizer is used to fractionate the cell membranes and then 3 min 600 g centrifugation was used to obtain Post-Nuclear Supernatants (PNS) ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in SDS lysis buffer (4% SDS, 20% glycerol, 125 mM Tris-HCl pH=6.8) supplemented with protease inhibitors (cOmplete ™ Mini (Roche)) ...
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bioRxiv - Genomics 2023Quote: One hundred mg of 14-day-old seedlings were ground and nuclei were isolated with 4°C Buffer (0,25M Sucrose, 10mM Tris-HCl, 10mM MgCL2, 1% Triton, 5mM ß- mercaptoethanol) containing proteinase inhibitor cocktail (Roche) and filtered in 63 µm ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were isolated by adding SDS lysis buffer (50 mM Tris-HCl pH 6.8, 2% SDS, 10% glycerol) supplemented with protease inhibitor cocktail (PIC; Roche). DNA was sheared by sonication for 10 min at high settings (30 s on ...
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bioRxiv - Cell Biology 2021Quote: ... Cell pellet samples were collected and re-suspended in lysis buffer (100 mM Tris-HCl, pH 7.4, 100 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, Roche complete protease inhibitor set ...
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bioRxiv - Biophysics 2022Quote: ... For cell lysis the cells were resuspended in 4 mL per g pellet in lysis buffer (20 mM Tris/HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, Protease Inhibitor (ROCHE), spatula DNAseI ...
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bioRxiv - Neuroscience 2022Quote: ... were mixed with an equal volume of 100 mmol/L Tris-HCl (pH 7.4) – 2% sarkosyl for 30 minutes prior to PK (200 μg/mL; Roche) digestion for 1 h at 37° C ...
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bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in buffer1 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Protease inhibitors (Roche)) and incubated for 20 min at 4°C ...
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bioRxiv - Pathology 2021Quote: ... adjust pH to 9.5 adding HCl) for 2 minutes and then tissue was covered with NBT/BCIP stock solution (Roche) diluted in 0.1M TRIS buffer (pH 9.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets resuspended in LB1 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2 supplemented with protease inhibitors [Roche]) for 5 minutes at 4 °C followed by centrifugation (1,000g ...
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bioRxiv - Microbiology 2021Quote: ... 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl PH8.0, 150 mM NaCl, complete protease inhibitor [Roche]), high salt (0.01% SDS ...
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bioRxiv - Physiology 2021Quote: ... Muscles were homogenized in buffer (2% sodium dodecyl sulfate, 50□mM Tris-HCl, pH 6.8) with protease and phosphatase inhibitors (Roche). Protein concentrations were determined by Bicinchoninic acid assay (Sigma-Aldrich ...
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bioRxiv - Physiology 2020Quote: ... were sonicated in lysis buffer (2% SDS, 60 mM Tris-HCl, pH 6.8) with phosphatase and protease inhibitors (Roche). Protein concentration was measured using a BCA assay (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... and the pellet was resuspended in 1-2 ml of lysis buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; protease inhibitor [Roche]), depending on the size of the cell pellet ...
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bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in buffer2 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Protease inhibitors (Roche), 0.5 % IGEPAL CA-630 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in 20 mM Tris-HCl (pH 7.4) with 2% SDS supplemented with a proteinase inhibitor (cOmpleteTM, EDTA-free Protease Inhibitor Cocktail, Roche). Protein concentration of lysates was measured using a bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2022Quote: ... Pellets were further extracted by sonication in 3x volumes of SDS lysis buffer (50□mM Tris-HCl, pH 7.4, 2% SDS, 1X Complete Mini protease inhibitor cocktail [Roche]) with centrifugation at 21,000 x g for 30 min at 25°C to obtain the SDS-soluble fraction (Triton-insoluble) ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections from proximity labeling experiments were lysed and de-crosslinked in 100 mM Tris-HCl buffer (pH = 8.0) with 2% SDS and protease inhibitor (Roche) at 95 °C for 45 min with constant shaking ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed in Laemmli buffer (62.5 mM Tris-HCl, pH 6.8, 10% glycerol, 2% SDS) supplemented with Complete® protease inhibitors (Roche), and sonicated before protein quantification (DC BioRad Protein Assay Cat No ...
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bioRxiv - Cell Biology 2023Quote: ... The clarified lysate was then diluted 3.75 times using dilution buffer (10 mM Tris–HCl, pH 7.4, 2 mM EDTA, 1 mM DTT, protease inhibitor cocktail tablet (cOmplete, Roche) and phosphatase inhibitor (phosSTOP ...
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bioRxiv - Developmental Biology 2023Quote: ... RPE explants were collected in 60 μl of lysis buffer (Tris-HCl 62.5 mM, SDS 2%, pH 6.8) with protease (Roche, #1183617001) and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: Cell lysis was performed by resuspending cell pellets in lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 0.5 mM DTT; 2 µM avidin, protease inhibitors cocktail (Roche) and DNAse (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... SDS (0.2%), Triton X-100 (2%), sodium deoxycholate (1%), Tris HCl (100 mM, pH 8.0)) with protease inhibitor (Roche #11836145001), then incubated on ice for 30 m ...
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bioRxiv - Biochemistry 2024Quote: ... and pellets were resuspended in lysis buffer (25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 0.5 mM TCEP, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]). The cells were lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 0.5 mM TCEP, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]), and lysed using a cell disruptor at 1.5 kbar ...