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Citations for Roche :
1 - 50 of 8565 citations for 2 6 Di 1 tert butyl 1 1 diphenylsilyl oxy 9 10 dihydroanthracene 9 10 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were reverse transfected with 1:3 or 1:6 X-tremeGENE 9™ (Roche) transfection reagent according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... (d) Remaining 9/10 of sample is immunoprecipitated with 1:1000 anti-HA antibody (0.1mg/mL Roche Rat Anti-HA High Affinity [11867423001] ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.3 % NP-40 and 1 x protease and phosphatase inhibitors, Roche, USA) by passing through a 23G needle and syringe 30 times and then left to stand on ice for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Parental and Haspin knockout cells stably expressing GFP-TAF5 were obtained by cotransfection with pcDNA5/FRT/TO/GFP-TAF5 (Antonova et al., 2018) and a pPGKpuro resistance plasmid (in a 9:1 ratio) using Xtreme Gene 9 (Roche). After 48 h ...
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bioRxiv - Microbiology 2020Quote: ... G355-5 cells were transfected with 1 to 6 µg DNA in 6 cm dishes using X-treameGENE 9 (Roche, Basel, Switzerland). The 293T/17 cell line was obtained from ATCC (CRL-11268 ...
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bioRxiv - Cell Biology 2024Quote: ... 6 uL X-tremegene-9 transfection reagent (Roche 06365787001), 0.3 μg pCMV-VSV-G (Addgene Plasmid #8454) ...
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bioRxiv - Microbiology 2022Quote: ... and 10 U mL−1 IL-2 (Roche) for 48 h at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... 2 million cells were seeded in a 10-cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... pH7.9, 1 mM EDTA, 1.5 mM MgCl2, 150 mM NaCl, 0.5% NP 40, 1 mM dithiothreitol, 1 × Roche protease inhibitor cocktail). The beads were eluted with 1xSDS sample buffer and subjected to SDS–PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... pH7.9, 1 mM EDTA, 1.5 mM MgCl2, 150 mM NaCl, 0.5% NP 40, 1 mM dithiothreitol, 1 × Roche protease inhibitor cocktail) and eluted with 30 µl of 2 × SDS sample buffer and subjected to western blot (WB ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin was washed five times with 10 CV of Nickel Buffer (25 mM HEPES pH 7.6, 0.3 M NaCl, 10 mM imidazole, 2 mM DTT, 0.01% IGEPAL CA-630, 10% glycerol, 1× Roche cOmplete protease inhibitors). Protein was eluted with Nickel Buffer supplemented with 300 mM imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transfected with Tau P301L-V5 encoding full length human tau with P301L mutation and V5 tag (GKPIPNPLLGLDST) and TFEB-GFP at a 2:1 ratio of tau:TFEB (X-tremeGENE 9, Roche). 24 hours later the media was changed and 40 µL of Pff was added to the culture along with 200 nM Bafilomycin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Ki-67 (rabbit monoclonal, clone name 30-9, Ventana Roche, 790-4286, 1:400), cleaved caspase 3 (rabbit monoclonal ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were immunostained for Ki67 (clone 30-9 at 1:7, Ventana- Roche), CD8 (clone SP57 at 1:7 ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10 µg sgRNA plasmid using X-tremeGENE™ 9 DNA transfection reagent (Roche 06365809001). One day after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... A ∼3:1 ratio of X-tremeGENE™ 9 DNA Transfection Reagent (Roche, XTG9-RO) was added to the mixture prior to incubation and application on HEK293T cells using standard methods 1 ...
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bioRxiv - Cell Biology 2022Quote: ... X-tremeGENE 9 (Roche) for sgRNA transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Xtreme gene 9 (Roche) reagent was used according to manufacturer’s recommendations for transient transfections.
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bioRxiv - Cell Biology 2023Quote: ... XtremeGene 9 (Roche Diagnostics) was used.
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bioRxiv - Cell Biology 2023Quote: ... Nuclei pellets were resuspended in 100 µl of nuclei lysis buffer (20 mM HEPES pH 7.9, 0.1 M NaCl, 1mM EDTA, 1 mM EGTA, 1mM DTT, 25 % glycerol, 0.5 mM AEBSF, 1×Roche complete protease inhibitor cocktail) for 20 min at 4 °C with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... with diluted XtremeGene-9 (Roche), per the manufacturer’s instructions ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... using X-tremeGENE 9 (Roche). SRE-luc vector (Ide et al. ...
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bioRxiv - Cell Biology 2022Quote: ... or with 1:10 Dnase I (stock 2 U/μL, Roche), and micrococcal nuclease (stock 2000 U/μL ...
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bioRxiv - Cell Biology 2023Quote: ... 10 ng.ml-1 insulin (Roche) and cultured on Collagen I (BD Biosciences ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.6; 15 mM NaCl; 10% glycerol; 0.5% Tween 20; 10 mM imidazole; 1 mM DTT; 20 mM NaHCO3; Roche protease inhibitors cocktail without EDTA) ...
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bioRxiv - Biophysics 2023Quote: ... 1% Triton X-100, 10% glycerol, 10 mM NaH2PO4, 10 mM NaP2O7, 100 μM ZnCl2, 10 mM NaF, 1 Roche protease inhibitor tablet), lysed by sonication and the supernatant isolated by centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... washing the pellet in PBS and resuspending the cells in ice-cold 10 ml buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1% NP-40, c0mplete Mini Protease inhibitor EDTA free from Roche) and rotating for 10 min at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Rap1GAP- RlucII/rGFP-CAAX + Gαi2 and D2 or PDZ-RhoGEF-RlucII/rGFP-CAAX + Gα13 and TPαR) using X-tremeGENE 9 DNA transfection reagent (3:1 reagent/DNA ratio; Roche) diluted in OptiMEM (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... PFN1-KO or PFN2-KO) were split 1:3 and the next day transfected using XtremeGene 9 (Roche) with 5 μg V5-NAA80 (M23L ...
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bioRxiv - Biochemistry 2023Quote: ... pCW57.1_yeastAOX 32 with the Delta-Vpr packaging plasmids and the VSV-G envelope plasmid (DNA concentration ratio, 1.2: 1: 0.3) into HEK293T cells using X-tremeGENE 9 Transfection Reagent (Roche). Lentivirus-containing media was harvested at 24 hr after fresh media change ...
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bioRxiv - Pathology 2024Quote: ... Slides were incubated for 30 minutes with 1 unit of DNase I (Roche, Cas. No. 9003-98-9) at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... using XTremeGene 9 transfection reagent (Roche). 12 hours after transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... 10% glycerol + 1:10 cOmplete mini protease inhibitor cocktail (Roche)) by incubation on ice followed by sonication (Bioruptor ...
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bioRxiv - Microbiology 2020Quote: ... and lysozyme (1 ml, 10 mg ml-1; Roche, Switzerland) were added to remove the OM and cell wall respectively ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μg ml−1 pepstatin (Roche), 10 μg ml−1 chymostatin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μg ml−1 leupeptin (Roche), 10 μg ml−1 pepstatin (Roche) ...
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: ... the 10% WST-1 (Roche, Switzerland) in medium was replaced in each 96 well ...
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bioRxiv - Plant Biology 2024Quote: ... 15 mM MgCl2, 1 mM EDTA, 10 % glycerol, 1 % Triton X-100, 10 mM β-mercaptoethanol, 2 mM PMSF and Roche cOmplete Protease Inhibitor Cocktail)) were added in the ground leaves and incubated for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... then resuspended in 4°C homogenization buffer (0.6 M sorbitol, 10 mM Tris pH 7.4, 1 mM EDTA, 1 mM PMSF, 2× Roche cOmplete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... and either pBABE-puro-F-NOMO1r (6 μg) or pBABE-puro-F-NOMO1r4-Mut (6 μg) using 27 µL of X-tremeGENE 9 (Roche). Supernatants were filtered after 72 h through a 0.45 μm filter unit ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected according to the manufacturer’s protocol at a µL lipofectamine: µg plasmid ratio of 3:1 (X-tremeGENE 9, Roche). After 48 hours ...
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bioRxiv - Genomics 2022Quote: ... 1 μL of 1:10 diluted cDNA was used in a 10 μL SYBR Green (Roche, 04887352001) qPCR reaction on a LightCycler 480 instrument (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in Immunoprecipitation buffer (150 mM NaCl, 10 mM Hepes, 2 mM EDTA, 1% Triton, 1.5 mM MgCl2, 10% glycerol, protease inhibitor from Roche) and centrifuged at 14,000 × g ...
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bioRxiv - Biophysics 2020Quote: ... the same procedure was used using different lysis (50 mM HEPES pH 7.4, 100 mM NaCl, 10% glycerol, 1 mM DTT, 1 mM ATP, 2 mM PMSF, 1 Roche tablet per 50 mL) and storage (50 mM Tris pH 7.4 ...
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bioRxiv - Plant Biology 2021Quote: ... 10% glycerol) containing 1 mM PMSF and 1× protease inhibitor (ROCHE) followed by incubation for 30 min at 4°C (shaking) ...
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bioRxiv - Neuroscience 2022Quote: ... 1% sarkosyl and protease inhibitor (1 tablet per 10 ml, Roche), and incubated for 60 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfection was performed using XtremeGENE 9 (Roche) on 15-cm dishes according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... and XtremeGENE 9 transfection reagent from Roche.