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Citations for Roche :
4901 - 4950 of 5179 citations for Human Suprabasin SBSN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... to evaluate library size and adapter dimer presence before the library concentration was determined via quantitative real time PCR using the KAPA library quantification kits (KK4824, Roche, Basel, Switzerland) on the QuantStudio7 or ViiA7 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies, Carlsbad, CA) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing ...
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bioRxiv - Bioengineering 2022Quote: ... Acetate concentration in the supernatant was measured using the Cedex Acetate V2 Bio HT kit on a Cedex HT system (Roche Custom Biotech). OD600 was measured using a Ultrospec 2100 Spectrophotometer (Amersham Biosciences).
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bioRxiv - Developmental Biology 2022Quote: ... Antisense and sense RNA probes of TaDOF5.6 and TaDOF3.4 genes were synthesized using a DIG RNA Labeling Kit (SP6/T7) (Roche, Cat No. 11175025910, USA). The oligonucleotide primers for in situ hybridization are listed in Table S28.
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bioRxiv - Genomics 2022Quote: ... The resulting libraries were purified following the Nextera XT protocol (0.6× ratio) and quantified by qPCR using Kapa Library Quantification kit (Kapa Biosystems, Cat. KK4824). Four plates were pooled together on a NextSeq 550 high-output flow cell or NextSeq 2000 P2-100 flow cell with 26 bp in read 1 and 50 bp in read 2 ...
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bioRxiv - Genomics 2022Quote: ... The amplification of 16S rRNA genes was conducted using the KAPA2G™ Robust HotStart ReadyMix PCR Kit (Kapa Biosystems, Wilmington, MA, USA) in a total volume of 25□μl ...
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bioRxiv - Plant Biology 2022Quote: ... The enriched biotinylated DNA fragments were subjected to library construction on beads using the KAPA HyperPrep Kit for Illumina (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: EMSA DNA fragments were labelled with digoxigenin (DIG) following the manufactureŕs instructions (DIG Gel Shift Kit 2nd Generation; Roche Diagnostics, IN, USA) and 30 fmol of labelled DNA was incubated with different amounts of purified HaWRI1_DBD recombinant protein (0-1000 ng ...
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bioRxiv - Plant Biology 2022Quote: ... The libraries were assessed for size and purity using the Agilent 2100 Bioanalyzer and quantitated by qPCR using a KAPA Biosystems Library Quantitation kit (Roche, Pleasanton, CA). Libraries were normalized ...
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bioRxiv - Microbiology 2022Quote: ... Cell equivalents were calculated according to amplification of the β-globin gene (two copies per diploid cell) with commercially available materials (Control Kit DNA; Roche Diagnostics). 2-LTR circles ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Immunology 2022Quote: ... cells were collected 24 h after incubation with 40 ng/ml IL-10 following the protocol of the High Pure RNA Isolation Kit (Roche, Basel, Switzerland).
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bioRxiv - Microbiology 2022Quote: ... then guide sequences were amplified from gDNA and the plasmid pool by nested PCR using KAPA HIFI Hotstart PCR kit (Kapa Biosystems, KK2501), as previously described in (Young et al. ...
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bioRxiv - Microbiology 2024Quote: ... Total nucleic acids were extracted from 200 µl of each sample using the DNA/Viral NA Small Volume v2.0 extraction kit (Roche Diagnostics, Mannheim, Germany) and an automated extractor MagNA Pure 96 ...
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bioRxiv - Physiology 2022Quote: ... The PCR products of target genes were purified and used as templates for probe preparation using DIG or fluorescein RNA labeling kits (Roche, Basel, Switzerland). The pituitary sections were hybridized with the antisense RNA probes for fshb and lhb as we previously reported [52] ...
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bioRxiv - Cell Biology 2022Quote: cDNA was synthesized from 100–500 ng of total RNA in 10 µl reactions with a Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol using random hexamer primers and an MJ Mini Personal thermal cycler (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System, Laval, QC, Canada) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Exome libraries were prepared from 1 μg of genomic DNA from each analyzed section using the Nimblegen EZ Exome kit V3 (Roche, Nutley, NJ). Paired-end 100 bp sequencing was performed on a HiSeq2500 sequencer (Illumina Inc. ...
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bioRxiv - Physiology 2024Quote: ... 20 ng cDNA was used for real-time qPCR reactions with KAPA SYBR FAST qPCR Kit (Catalog no. KK4618, Roche, Basel, Switzerland) using a CFX Opus Real-Time PCR System (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2024Quote: ... Gene-specific antisense or sense DIG-labeled cRNA probes were obtained by in vitro transcription with T7 polymerase using the Roche DIG RNA labeling kit (Roche Diagnost ics) following the instructions of the supplier ...
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bioRxiv - Cancer Biology 2024Quote: ... size and concentration of cDNA libraries were checked using the High Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Novaseq 6000 sequencer as single-end 100 base reads ...
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bioRxiv - Neuroscience 2024Quote: ... cDNAs and final libraires were run on an Agilent 4200 TapeStation to check for quality and were quantified using KAPA Library Quantification Kit (Roche, Basel, Switzerland).
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bioRxiv - Genomics 2024Quote: ... Hybridization was carried out with SeqCap RNA Choice Probes using the KAPA HyperCapture Reagent and Bead kit (v2, Roche Sequencing Solutions, Inc.) according to SeqCap RNA Enrichment System User’s Guide (v.1.0 ...
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bioRxiv - Cancer Biology 2024Quote: Terminal dUTP Nick End Labeling (TUNEL) staining was performed with an In Situ Cell Death Detection Kit (TMR Red #12156792910; Roche Applied Science) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Illumina-compatible sequencing libraries were prepared from 20 ng of purified DNA in 50 μL of TE buffer using the KAPA Hyperprep Kit with Library Amplification Primer Mix (Roche, CAT #07962363001) and 6 cycles of PCR amplification ...
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bioRxiv - Cancer Biology 2024Quote: ... we treated sensitive and resistant cells with increasing concentrations of SP600125 for 24 hours and evaluated the cell viability using the Cell Proliferation Kit I (MTT) (Roche, Cat. 11465007001) and measuring the absorbance value at λ=590nm ...
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bioRxiv - Immunology 2023Quote: ... The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Roche, cat#07960140001). Individual libraries were normalized to 5 nM in preparation for Illumina sequence analysis and sequenced on a NovaSeq 6000 with a paired-end 150 cycle sequencing run.
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bioRxiv - Developmental Biology 2022Quote: ... and incubated at 37 °C in the dark for 1 h with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) solution from the In Situ Cell Death Detection Kit (Roche, Mannheim, Germany). The embryos were washed three times with PBS-PVP ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was isolated by RNA Premium Kit (NIPPON Genetics, Cat# FG-81250) and cDNA was synthesized by Transcriptor Reverse Transcriptase (Roche, Cat# 03531287001) with random hexamer ...
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bioRxiv - Cancer Biology 2023Quote: ... Strand-specific and barcode-indexed RNA-Seq libraries were generated from 300 ng total RNA each after poly-A enrichment using the mRNA-Seq Hyper Kit (Kapa Biosystems, KK8581) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng of purified DNA for each IP and input sample were used for library preparation with the KAPA Hyper Prep Kit (Kapa Biosystems KR0961). TAZ and Combo treatments required two IPs per biological replicate to attain enough material for library preparation ...
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bioRxiv - Microbiology 2023Quote: For NGS library preparation we added adapters and unique dual indices in a two-step amplification process using the KAPA HiFi HotStart Library Amplification Kit (KAPA Biosystems, KK2611) and purified samples with Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... the cDNA inserts of the plasmids were isolated and amplified by low-cycle (12x) PCR using a Kapa2G Robust Hotstart PCR kit (Roche, Ref #07961073001) according to specifications ...
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bioRxiv - Microbiology 2023Quote: ... supernatant from the cells was collected and used to quantify LDH release using the LDH cytotoxicity detection kit (Roche, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... or T3 polymerase (pSC-best4, pSC-otop2) and fluorescein or digoxygenin RNA labeling kits (digoxygenin: Roche, Cat# 11277073910; fluorescein: Roche, Cat# 11685619910) These reactions were cleaned using the NEB Monarch RNA Cleanup protocol (NEB ...
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bioRxiv - Pathology 2023Quote: Terminal deoxynucleotidyl transferase-mediated digoxigenin-deoxyuridine nick-end labeling (TUNEL) staining was performed by using the In Situ Cell Death Detection Kit from Roche (Mannheim, Germany). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The libraries obtained from the CBE approach were quantified using the qPCR KAPA library quantification kit (Roche Diagnostics, Cape Town, South Africa). Sample size of all libraries was checked using an Agilent 2100 Bioanalyzer (Agilent technologies ...
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bioRxiv - Genomics 2023Quote: ... Then DNA fragments underwent a NGS library preparation procedure consisting in end repair and Illumina adaptor ligation using the KAPA HTP kit (Roche, Basel, Switzerland). Individual index sequences were added to each library for identifying reads and sorting them according to their initial origins ...
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bioRxiv - Genetics 2023Quote: ... and ceramic beads (Precellys Lysing Kit CkMix, Precellys) in lysis buffer (Lysis Buffer 1X, Milliplex) to which a cocktail of protease inhibitors (Roche Complete Mini) and phosphatase inhibitors (PhosSTOP EASYpack ...
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bioRxiv - Cancer Biology 2023Quote: ... 300 µL of the aqueous phase was mixed with 375 µL ethanol and loaded onto a column from the hiPure miRNA purification kit (Roche, Cat. 5080576001). The samples were then washed and eluted according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: Apoptotic cells present in gonadal histological preparations were revealed by the terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labelling (TUNEL) assay using the Fluorescent In Situ Cell Death Detection Kit (Roche ref. 11684795910, Roche, Mannheim, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: The TUNEL assay was performed to detect apoptosis using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and In-Situ cell death detection Fluorescein kit (Roche Cat#11684795910), as published ...
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bioRxiv - Genomics 2023Quote: Approximately 500ng Total RNA was used for RNA-seq library preparation by following the KAPA Stranded RNA-Seq Kit with RiboErase (HMR) sample preparation guide (Cat. No: KR1151, KAPA Biosystems, USA). The first step in the workflow involved the depletion of rRNA by hybridization of complementary DNA oligonucleotides ...
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bioRxiv - Immunology 2023Quote: Min6.luc and Min6.CD44 cells were harvested for total RNA isolation using the High Pure RNA isolation kit (Roche Applied Science) and reverse-transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR was performed using gene-specific qPCR primers listed in Supplementary Materials and the KAPA SYBR Fast qPCR Master Mix (2X) kit (Kapa Biosystems, #KK4602), and ran on CFX96 Real-Time PCR Detection System (BIO-RAD ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Whole-genome libraries were prepared from sheared samples using the KAPA Hyper Plus library preparation kit (KK8514, Roche Diagnostics, Santa Clara, California), NEBNext Multiplex Oligos for Illumina (E7600 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized by a reverse transcriptase reaction with 500 ng of total RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basal, Switzerland), and was used as a template for qPCR with LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Microbiology 2023Quote: ... a 710 bp region was amplified by PCR using previously described primers (45) and the KAPA HiFi HotStart ReadyMix PCR Kit (Roche, Basel, Switzerland) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Average library size and quality control were performed using a Fragment Analyzer (High Sensitivity NGS kit) and qPCR (Roche Light Cycler 480). CUT&RUN libraries were sequenced on a NovaSeq 6000 (Illumina ...