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Citations for Roche :
4801 - 4850 of 8657 citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed for 10 min on ice in 3 ml ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris pH 8.1, 1 mM PMSF, 1,5x Roche protease inhibitor mix). The DNA was sheared by sonication (4x 7 cycles ...
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bioRxiv - Genomics 2024Quote: The nuclei were resuspended in 250 µL of 5’ phosphorylation master mix (1X T4 PNK buffer, 500 U/mL of T4PNK, 1 mM ATP, 1 U/µL of RNAse inhibitor (Roche, 3335399001)) and incubated at 37°C while rotating at 800 rpm for 1 hour to phosphorylate the 5’ ends of RNA ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM EDTA, 1 mM Na3VO4, 20 mM NaF, 0.5% Triton X-100, 1 × protease inhibitors complete EDTA-free from Roche, Mannheim, Germany). Total protein concentration of prepared cell extracts was measured by Bio-Rad protein assay (Bio-Rad laboratories GmbH ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% bovine serum albumin (BSA)/TBS-T ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.9, 50% glycerol, 75 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 1 mM PMSF and Roche protease inhibitor cocktail), then 100 μl prechilled nuclei lysis buffer (10 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in ice-cold PBS and lysed (1% SDS, 10 mM Tris-HCl, 1 mM NaVO3, cOmpleteTM Mini Protease Inhibitor (Roche, #11836153001), pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche] in Maleic acid) for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1% SDS) with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 X EDTA-free mammalian protease inhibitor cocktail (Roche Diagnostics, 11836170001). Cell pellets from control and subject fibroblasts and corresponding overexpression cells were solubilized in 1% n-dodecyl-β-D-maltoside (DDM ...
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bioRxiv - Developmental Biology 2023Quote: ... cut into small pieces with a scalpel and transferred into 1 ml collagenase-dispase solution (1 mg/ml in PBS, Roche #10269638001) for 10 min at 28°C ...
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bioRxiv - Molecular Biology 2023Quote: ... extract was prepared using Co-IP buffer (50 mM Tris/Cl (pH=7.5), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 10% glycerol, 1xPIC (cOmplete™, Protease Inhibitor Cocktail (Roche; 04693116001), 0.5 mM PMSF) ...
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bioRxiv - Plant Biology 2023Quote: ... 50 mM Tris/HCl pH7.5, 1 mM EDTA, 300 mM NaCl, 1 mM DTT, 0.1% [v/v] Nonidet P-40/Igepal, Roche cOmpleteTM protease inhibitor) was added to grinded-frozen samples ...
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bioRxiv - Genetics 2023Quote: ... 25-30 million cells were resuspended in 1 mL lysis buffer (1% SDS, 50 mM Tris-HCl pH 8, 20 mM EDTA, 1x cOmplete (Roche, 4693132001) protease inhibitor) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The libraries were diluted 1:10 000 and 1:20 000 and quantified in triplicate using the KAPA Library Quantification Kit (Kapa Biosystems). Equimolar samples were pooled ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.4 with 0.1% Triton X-100) and then incubated with 350 µg protein in 1:1 8M urea with Complete protease inhibitor (Roche 11245200) sample lysis:Buffer A/0.5% Triton X-100 (150 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were prepared by incubation in Onyx lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche Molecular Biochemicals]) at 4 °C for 15 min ...
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bioRxiv - Biophysics 2024Quote: ... Cultures were grown until an A600 of 0.6-0.8 was reached and protein over-expression was induced by incubating with 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG, Roche) for 16 hours at 18°C.
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 1.5h in PBSTw-1%BSA before being incubated with anti-DIG-POD antibody (Roche, 1:2000) and other primary antibodies o/n at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... 1 mM phenylmethyl sulphonyl fluoride (PMSF)) plus EDTA-free protease inhibitor tablets (1 tablet per 50 mL, Roche, cat. no. 05056489001), 0.5% Triton-X100 ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... Cells were grown to ∼80% confluency, washed in PBS and lysed (1% SDS, 10 mM Tris-HCl, 1 mM NaVO3, and protease inhibitors (Roche, #11836153001), pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR was performed using the extracted DNA (100 ng) as template with Kapa SYBR Fast qPCR Kit Master Mix (2×) Universal (Kapa Biosystems Ltd., Wilmington, MA, USA) on a CFX connect real-time system (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2019Quote: ... the cDNA samples were diluted with 220 µl distilled water and 2 μl aliquots were amplified with the LightCycler® Nano Real-time PCR Detection System (Roche Applied Science, Tokyo, Japan) using the KOD SYBR® qRT-PCR Mix (TOYOBO) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5X SSC, 5X Denhardt’s solution, 200 μg/ml yeast RNA, 500 μg/ml salmon sperm DNA and 2% Roche blocking reagent in DEPC treated water). Incubation with pre-hybridization buffer was carried out during 4h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 50% formamide, 5X SSC, 5X Denhardt’s, 200 μg/ml yeast RNA, 500 μg/ml salmon sperm DNA and 2% Roche blocking reagent in DEPC treated water) containing biotin and/or digoxin labeled Locked Nucleic Acid (LNATM ...
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bioRxiv - Cancer Biology 2021Quote: ... The whole cell lysates were extracted from trans transfected 293T cells or infected LNCaP cells or PC-3 cells by IP lysis buffer (50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, Roche protease inhibitor cocktail). An aliquot of the cell lysate was kept as input for western blot analysis ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol and Roche Complete Protease Inhibitors) and homogenized using a high-performance disperser (Fisher) ...
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bioRxiv - Cell Biology 2020Quote: HEK293T cells were lysed in 1% DMNG buffer (150mM NaCl, 50mM Tris-HCl pH 7.5, 5mM MgCl2, 1% DMNG, protease inhibitors (Roche diagnostics, EDTA free) for 90 min ...
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bioRxiv - Cell Biology 2022Quote: ... cells were first thawed on ice and resuspended in 1 mL FA buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% sodium deoxycholate, with Roche cOmplete protease inhibitors (Sigma)) ...
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bioRxiv - Biochemistry 2019Quote: ... from 4L of bacterial culture was resuspended in 30 mL lysis buffer (50 mM Tris-HCl pH 8.3, 1 mM EDTA, 150 mM NaCl, 10% glycerol, 5 mM DTT, 1 mM PMSF, and Roche protease inhibitor cocktail) and sonicated on ice ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol and Roche Complete Protease Inhibitors) and homogenized using a high-performance disperser (Fisher) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were gently resuspended in 25 ml of swelling solution (1 mM PIPES pH 7.0, 1 mM MgCl2, 5 μg/ml taxol and Roche Complete Protease Inhibitors) and immediately centrifuged at 250 g for 3 min ...
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bioRxiv - Plant Biology 2021Quote: ... Three volumes of lysis buffer (50 mM Tris-Cl pH 7.4, 150 mM KCl, 1% Triton-X100, Protease inhibitor 1 X [Roche], NEM 20 μM) was added to 2 g of powdered tissue ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5 mM MgCl2, 1 mm EDTA, 0.5 mM DTT, 50 mM NaF, 50 mM sodium pyrophosphate, 1 mM Na3VO4 and Roche protease inhibitor cocktail) by glass bead lysis ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were collected via centrifugation and lysed in lysis buffer (1.5M KCl, 20 mM Tris pH 8.0, 20 mM Imidazole pH 8, 1 mM DTT, 1 tablet of Roche protease inhibitor cocktail). Sonication was performed to further lyse cells and to shear DNA ...
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bioRxiv - Biophysics 2020Quote: ... from 18-day old mouse pups were disrupted using forceps and a razor blade in 1 mL of 1× PBS with protease inhibitors (05896988001, Roche Complete Ultra). The cell suspension was then gently added to a 15-mL conical tube with 5 mL of 1× PBS with protease inhibitors and allowed to settle ...
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bioRxiv - Molecular Biology 2022Quote: ... The adhered cells were then gently washed with cold PBS and lysed in 1% NP-40 lysis buffer (50mM Tris-HCl pH 8.0, 150 mM sodium chloride, 1% NP-40, Roche Complete protease inhibitor). The lysate was then cleared by centrifugation at 17,000 x g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2% NP-40, 10% glycerol, 1 mM EDTA, 1 mM PMSF, 20 µM MG132, 5 mM DTT and Roche protease inhibitor #5892953001), and incubated for 1 h on a rotating wheel ...
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bioRxiv - Plant Biology 2021Quote: Frozen inflorescence tissue was ground with liquid nitrogen and resuspended in lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5 mM DTT, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting nuclear pellet was resuspended in wash buffer (10 mM Tris pH 8, 0.25 M sucrose, 10 mM MgCl2, 1 mM EDTA, 1% Triton X-100, 1X Roche protease inhibitor cocktail) and nuclei were cleaned and pelleted at 12,000 x g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were pelleted and washed with 1 pellet volume of cold RP buffer (retromer purification buffer: 50 mM Tris pH 8.0, 300 mM NaCl, 1 mM MgCl2, 1 mM PMSF, Roche complete protease inhibitor).
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bioRxiv - Developmental Biology 2022Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche, 11175041910] in Maleic acid) for 1 h ...
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bioRxiv - Genetics 2019Quote: ... resuspended in 60 ml hypotonic lysis buffer (20 mM HEPES-KOH pH 7.5, 5 mM NaCl, 1 mM MgCl2, 1 mM PMSF and EDTA free protease inhibitor mixture from Roche and Thermo Scientific) and incubated for 15 min on ice ...
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bioRxiv - Immunology 2019Quote: ... and nuclei were collected and re-suspended in 300μl SDS lysis buffer (1% SDS, 1% Triton X-100, 2mM EDTA, 50mM Hepes-KOH [pH 7.5], 0.1% sodium deoxycholate, Roche 1X Complete Protease Inhibitor). Nuclei were lysed for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 mM MgOAc, 15 mM Tris-HCl pH 7.4, 0.5% NP40, 1 mM DTT, 1 μl RNAsin, Roche protease inhibitors without EDTA). The cells were lysed by one freeze-thaw cycle in large volume (15–50 ml ...
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bioRxiv - Cell Biology 2020Quote: ... The remaining cell pellet was weighed and resuspended in a 1:1 ratio with ice-cold 1x PBS with a protease inhibitor tablet (Roche Life Sciences) and 1mM PMSF ...
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bioRxiv - Immunology 2020Quote: ... cut into small pieces and digested for another 1 h at 37°C in 1 mg/ml Collagenase D (Roche, Basel, Switzerland). To obtain single-cell suspensions ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... The cells were lysed using RIPA buffer (Tris-HCl 10 mM, 1% SDS, 10 mg/ml deoxycholate, 150 mM NaCl, 1% NP40 and protease inhibitors cocktail [Roche, Mannheim, Germany]). The total protein samples were separated using by 12% SDS-PAGE and transferred onto nitrocellulose membranes (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... three hearts per experimental group were homogenized into ice-cold isolation buffer (300 mM KCl, 30 mM PIPES pH 6.6, 0.5% NP-40, 1× protease inhibitor [Roche], 1× phos-stop [Roche]), followed by centrifugation at 15,000 rpm for 10 minutes in a tabletop centrifuge at 4°C to remove insoluble proteins ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM β-glycerophosphate and 1% Triton) containing protease inhibitor cocktail (Rowe Scientific; CP2778) and PhosSTOP EASYpack Phosphatase Inhibitor Cocktail (Roche; 4906837001) on ice for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Five hunderd mL Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% SDS, 1 mM PMSF, and EDTA-free protease inhibitor cocktail from Roche, dissolved in PBS) was added and cells were harvested from the Transwell membrane using a disposable cell scrapper and transferred to an Eppendorf tube ...
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bioRxiv - Synthetic Biology 2022Quote: ... 140 mM NaCl, 1mM ethylenediaminetetraacetic acid, 1% Triton X-100, 0.1% Na-Deoxycholate, 1 mM phenylmethylsulfonyl fluoride, 200 µL Roche cOmplete protease inhibitors). 0.5 mm diameter glass beads were added to 1 mm below the meniscus ...