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Citations for Roche :
4751 - 4800 of 6180 citations for Glucagon like peptide 1 receptor GLP1R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was retrieved into a new 2 ml Eppendorf tube and diluted 10 times with RIPA buffer 0% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, protease inhibitor cocktail (Roche)) to obtain a concentration of 0.1% sodium dodecyl sulfate ...
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bioRxiv - Developmental Biology 2022Quote: ... and then homogenized with a glass tissue homogenizer in lysis buffer (50 mM Tris pH 7.5, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate) containing protease inhibitors (Roche, 11697498001). Supernatant fractions were collected by centrifugation and boiled with 2× Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 488 fluorophores (dilution 1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... the instruments assessed across the four test scenarios were the cobas connection modules (CCM, scenario 1; Roche Diagnostics International), manual output station 1 (MO1 ...
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bioRxiv - Cell Biology 2022Quote: ... the dorsal skin of C57BL/6 neonates was collected and incubated overnight with 1 mg/mL collagenase/dispase (Roche). The dermis and epidermis were separated by incubating the skin with 0.25% trypsin/10 mM EDTA in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellets were resuspended in lysis buffer (50mM HEPES pH 7.4, 100mM KAc, 50mM NaCl, 10mM MgAc, 1% digitonin, 1X Roche protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% NP-40, 2x Roche EDTA-free protease inhibitors ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.5, 400 mM NaCl, 5% glycerol, 20 mM imidazole, 1 Mm DTT, Complete EDTA-free protease inhibitor [Roche]) and the cleared lysate was incubated with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then permeabilized with 0.5% TritonX- 100 for 15 minutes at room temperature and incubated with 1mg ml−1 DNase I (Roche) for one hour at 37 °C followed by incubating with anti-BrdU (BU20A ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was briefly rinsed with 1 x TBE and RNA was transferred to positively charged Nylon membrane (Roche) using Fastblot (Biometra ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies used for western blots presented in this work were as follows: anti-HA monoclonal antibody (mAb) 3F10 (diluted 1:2,000) (Roche); mouse anti-GAPDH mAb (1:20,000) ...
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bioRxiv - Immunology 2022Quote: ... The cells were lysed in TBS/1% (v/v) IGEPAL-630 (sigma) containing 1X Complete protease cocktail inhibitors (Roche). The TCR-CD3 complex was purified from the lysate using Dynabeads M-280 streptavidin (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... The supernatant was then diluted ten times in column buffer (20 mM Tris ph 7.4, 200 mM NaCl, 1 mM EDTA, 1X Protease inhibitors (catalog no. 11873580001, Roche)) ...
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bioRxiv - Physiology 2022Quote: ... Cleared extracts were diluted ten times in column buffer (20 mM Tris ph 7.4, 200 mM NaCl, 1 mM EDTA, 1X Protease inhibitors (catalog no. 11873580001, Roche)) ...
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bioRxiv - Physiology 2022Quote: ... Cells were then pelleted at 300g for 5 min and lysed in lysis buffer (20 mM Tris ph 7.4, 200 mM NaCl, 1 mM EDTA, 1X Protease inhibitors (catalog no. 11873580001, Roche), 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2022Quote: ... buffer (50mM Tris-HCl pH = 7.4, 1% NP-40 / Igepal, 100mM NaCl, 2mM MgCl2) supplemented with Proteinase Inhibitor (Roche) und Phosphatase Inhibitor (ThermoScientific) ...
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bioRxiv - Microbiology 2022Quote: ... Two µg plasmid DNA and 25 µL DOTAP (N-[1-(2,3- Dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate) (Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Genomics 2022Quote: ... Beads were washed twice 10 min with cold buffer I (150 mM NaCl, 1% Triton X-100, 0.1% SDS, 2 mM EDTA, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were then blocked for an hour in 5% milk powder/PBS solution and probed with 1:2,500 rat anti-HA mAb 3F10 antibody (Roche) in 5% milk powder/PBS solution overnight ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Transfected Expi293F cell pellets were lysed in PBS + 1% Triton-X100 and Complete protease inhibitor tablets (Roche, Mannheim, Germany) for 15 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: ... lung samples of infected mice were harvested and homogenized in 1 ml PBS using the MagNA Lyser (Roche Diagnostics). The homogenates were clarified by centrifugation at 15,000 rpm for 5 min ...
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bioRxiv - Genetics 2022Quote: ... 1 mM MgCl2 10 µM ZnSO4) the buffer was also supplemented with both cOmplete EDTA-free protease inhibitor (Roche) and Benzonase (Merck) ...
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bioRxiv - Cell Biology 2022Quote: ... 1% lauryl maltose neopentyl glycol (LMNG)) containing a mix of proteases inhibitors (cOmplete Tablets, Roche, 04 693 124 001). After centrifugation at 5,000 g for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 100 mM Tris pH 8.0 with 1% SDS supplemented with protease inhibitor (Complete EDTA-free, Roche). Cells in lysis buffer were heated 3 times for 5 min at 95 °C ...
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bioRxiv - Microbiology 2022Quote: Fresh pre-cut pancreatic tissue (2 × 2 mm) were digested with a solution of collagenase P in HBSS-1% HEPES (0.75 mg/mL, Roche) at 37°C for 7 min with shaking ...
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bioRxiv - Immunology 2022Quote: ... diluted 1:3,000 in 1% BSA in PBS which was then detected using an ABTS substrate solution complimented with H2O2 (Roche). The optical density (OD ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed 2x in PBS + 0,1% Tween-20 (PBS-T) followed by 15 min permeabilization in 1- μg/ml proteinase K (Roche) and 3 washes in PBS-T ...
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bioRxiv - Cell Biology 2022Quote: Cell pellets were lysed for 30 min at 4°C with end-over-end mixing in cold lysis buffer [50 mM tris pH 7.5 | 150 mM NaCl | 1 mM EDTA | 0.5% NP-40 | 1x protease (Roche, 11836170001) and phosphatase (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... cells were extracted using NETN buffer (20 mM Tris at pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% Igepal, 1.25 mM DTT, Roche protease inhibitor) with several freeze/thaw cycles ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of bromophenol blue and 50 mM DTT) containing 2 mM PMSF and cOmplete protease inhibitor cocktail (Roche) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS and 1% sodium deoxycholate) supplemented with protease and phosphatase inhibitors (100 mM PMSF and PhosSTOP tablet (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: ... DRGs were dissociated in 1 μg/ml collagenase A plus 2.4 U/ml dispase II (enzymes, Roche Applied Sciences) in HEPES-buffered saline (HBSS ...
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bioRxiv - Cell Biology 2021Quote: ... beads were washed with150 μL cold BSA/PBS for 3 times and mixed with 100 μL Binding Buffer (1% Triton X-100, 0.1% Sodium Deoxycholate, 1x complete protease inhibitor (Roche)) plus 100 μL 0.2 μg/μl chromatin followed by overnight incubation on a rotating platform at 4°C ...
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bioRxiv - Microbiology 2020Quote: 0.5 to 1 million cells were lysed in 100 μL of RIPA buffer (50mM Tris HCl, 150mM NaCl, 0.1% SDS, 0.5% DOC, 1% NP-40, Protease inhibitor (Roche; 1187358001)) ...
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bioRxiv - Neuroscience 2020Quote: ... Powdered brains were then resuspended in 1mL ice cold 1X Lysis Buffer(50mM Tris-HCl pH 7.4, 100mM NaCl, 1%NP-40, 1X Roche c0mplete EDTA-free protease inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... 300 mM NaCl, 30 mM imidazole, 1 μM DNases, 0.75% CHAPS, 5[mM MgCl2 and EDTA-free protease inhibitor cocktail [Roche]), and lysed with high pressure cell homogenizer (Union-Biotech ...
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of the identified drugs on Vero E6 cell was determined with WST-1 cell proliferation assays (ROCHE, 5015944001). Twenty thousand cells were seeded into a 96-well plate and incubated for 20–24 h at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in 5% milk and probed overnight at 4°C with 1:2,500 rat anti-HA mAb 3F10 (Roche). They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 l E.coli culture was harvested and re-suspended in the lysis buffer (50mM HEPES pH7.5, 150mM NaCl, 5mM MgCl2, 1% Triton X-100, EDTA-free protease inhibitor cocktail (Roche) and 2mM PMSF (Sigma/Merck) ...
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bioRxiv - Genomics 2021Quote: ... and then resuspended in 3 mL of lysis buffer (160 nM NaCl, 20 mM, Tris-HCl pH7.5, 1 mM EDTA, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biophysics 2020Quote: ... the pool was loaded in 0.5 mL GST resin mixed with 1 mL of cOmplete His-tag purification resin (Roche) stacked in a 12 mL polyprep column ...
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bioRxiv - Neuroscience 2020Quote: Whole cerebral organoids were washed three times in 1× PBS and dissociated in 2 mL of Accutase (StemPro) containing 0.2 U/μL Dnase I (Roche) for 45 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... 200 μg of mitochondria were lysed in 20 μL of lysis buffer (50 mM KPi pH 7.4, 150 mM KCl, 1× Complete Protease Inhibitor cocktail (Roche), 1 mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... human iPSC-derived neuronal cultures and N2a cells were collected in Lysis Buffer (50 mm Tris-Base, 150 mm NaCl, 1% Triton X-100, 0.5% deoxycholic acid) with protease inhibitor (Roche) and phosphatase inhibitor cocktails II and III (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... protein extracts were obtained by lysing cells in lysis buffer (50 mM Tris-pH: 7.5, 150 mM NaCl, 1% Triton-X100, 1x Complete Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X- 100, 2 mM EDTA, 20 mM Tris, pH 8.1, 150 mM NaCl, and protease inhibitors; Roche), and then washed once with 1 ml of “high-salt buffer” (the same but with 500 mM NaCl ...
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Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... The slides were incubated overnight with a 1:5,000 dilution of alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics). After the slides had been washed in in a solution of 0.1 M Tris (pH 7.5 ...
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bioRxiv - Physiology 2021Quote: ... Reactions were performed in a 6 μl volume of 1 x Kapa Probe Fast Mastermix (Kapa Biosystems, Amsterdam, Netherlands). TaqMan assays (FAM labelled ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in KPBS and incubated with rabbit anti-FG antibody (1:5000; Chemicon International, Calif, USA) in 0.5% Blocking Reagent (Roche) in KPBS for 16 h at 4 ° C ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: ... The membrane was blotted overnight at 4°C with rat antibody against the HA tag (Roche, 1/500 dilution), TRAF2 and TRAF6 rabbit antibodies (Suppl ...