Labshake search
Citations for Roche :
4751 - 4800 of 10000+ citations for Cow Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... and quantified using real time PCR with an NGS Library Quantification Kit (Roche/Kapa Biosystems) on a StepOnePlus Real Time PCR Workstation (Thermo/ABI).
-
bioRxiv - Neuroscience 2024Quote: Adenosine 5’-triphosphate (ATP) levels were assessed using an ATP Bioluminescence assay Kit (Sigma/Roche). Briefly ...
-
bioRxiv - Cell Biology 2024Quote: ... libraries for RNA-Seq were prepared with KAPA Stranded mRNA-Seq Kit (KAPA Biosystems #KK8421). Briefly ...
-
bioRxiv - Cancer Biology 2024Quote: ... Libraries of germline DNA were prepared using KAPA Hyper Prep Kit (Roche Diagnostics, Branchburg NJ). Libraries were subjected to targeted next generation sequencing (NGS ...
-
bioRxiv - Developmental Biology 2024Quote: ... TUNEL staining was performed using the In Situ Cell Death Detection Kit (Roche, Cat #11684817910) according to the manufacturer’s specifications ...
-
bioRxiv - Cell Biology 2024Quote: ... maxi- and minicircles were PCR amplified and labelled using PCR DIG Probe Synthesis Kit (Roche). The detection was done using DIG Luminiscent Detection Kit (Roche ...
-
bioRxiv - Biochemistry 2024Quote: ... The LDH assay was performed using the Pierce LDH Cytotoxicity Detection Kit Plus (Roche, 04744926001). Briefly ...
-
bioRxiv - Cancer Biology 2024Quote: ... then libraries were prepared using the KAPA Hyper Library Preparation Kit (Roche Sequencing Solutions, Inc.). The libraries were amplified with 3-8 cycles of PCR ...
-
bioRxiv - Cancer Biology 2024Quote: ... The pool was then quantified by qPCR using the KAPA Library Quantification Kit (KAPA Biosystems) and sequenced on the Illumina NovaSeq6000 S4-300 flow cell using the 150nt paired end format.WGS with low input material was carried out using Illumina NovaSeq with 150 bp paired-end sequencing ...
-
bioRxiv - Cancer Biology 2024Quote: The reverse transcription reaction was proceeded using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... DNA detection was performed using DIG High Prime DNA Labeling and Detection starter kit (Roche). The HR sequences were amplified from pSLI-SUN1-HAx3-glms and pSLI-SUN2-HAx3-glms ...
-
bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were carried out in 15 µl using the Taq DNA Polymerase kit (Roche) and the following conditions ...
-
bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (cat. 08098131702, Roche) and purified by AMPure SPRI beads (cat ...
-
bioRxiv - Physiology 2024Quote: ... Digoxigenin (DIG)-labeled probes were synthesized with a DIG RNA Labeling Kit (Roche Applied Science). The paraffin sections were made as described above and subjected to in situ hybridization ...
-
bioRxiv - Genetics 2024Quote: ... Digoxigenin (DIG)-labeled in-situ probes were synthesized using DIG RNA Labeling Kits (Roche, 11277073910). RNA in-situ hybridization was carried out as previously described (Thisse and Thisse ...
-
bioRxiv - Genomics 2024Quote: ... Isolates were then prepared for sequencing using the KAPA Hyperprep kit (Kapa Biosystems, Woburn, MA). Four Tanzanian DNA isolates extracted from blood that had been leukocyte-depleted by CF11 filtration at the time of collection25 were directly incorporated into sequencing libraries ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Reverse transcription into cDNA was performed using the Transcriptor Reverse Transcriptase kit (Roche, Indianapolis, IN) and random hexamer primers (Roche ...
-
bioRxiv - Neuroscience 2024Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
-
bioRxiv - Microbiology 2024Quote: ... PCR was done with the Kapa HiFi PCR kit (KAPA Biosystems, Cape Town, South Africa) using 5 μl of extracted DNA as template and the following cycling conditions ...
-
bioRxiv - Plant Biology 2024Quote: ... Libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems). Equimolar amounts of each library were used for cluster generation on the cBot using the Illumina TruSeq Paired-End Cluster Kit v3 ...
-
bioRxiv - Neuroscience 2024Quote: ... and a qPCR-based KAPA library quantification kit (KAPA Biosystems, Wilmington, MA, USA, Cat#KK4600) were employed ...
-
bioRxiv - Immunology 2024Quote: ... The concentrations of the pools were measured with the Illumina Library Quantification Kit (KAPA Biosystems) and sequenced on the Illumina HiSeq 4000 genome sequencer using 75 bp paired-end SBS chemistry ...
-
bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was then synthesized using a Transcriptor First Strand cDNA Synthesis kit (Roche). qPCR analyses were performed using FastStart Essential DNA Green Master mix (Roche ...
-
bioRxiv - Cell Biology 2020Quote: ... A 2ml Dounce homogenizer was used to individually homogenize each brain in a 1:1 solution of Lysis-R:2X-RIPA buffer solution with protease inhibitors (Roche cOmplete tablets #11836153001). Each sample was sonicated three times for 7 seconds and then centrifuged at 5000g for 5min at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... They were allowed to equilibrate in rigor buffer (20 mM HEPES pH 7, 140 mM KCl, 2 mM MgCl2, 1 mM EGTA, 1 mM DTT, Roche complete protease inhibitor) over night at 4 °C ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples were then incubated overnight at 4°C in primary antibody dilutions in freshly prepared BBT+ buffer (PBST + 1% BSA + 0.5 mM Spermidine + 2 mM EDTA + 1 large Roche complete EDTA-free tablets). Primary antibody was replaced with BBT+ buffer and quickly washed twice ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were harvested and resuspended in binding buffer (100 mM Tris- Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, complete protease inhibitor cocktail (Roche; 1 tablet/10 mL) before sonicating on ice ...
-
bioRxiv - Biochemistry 2021Quote: Bacteria were resuspended in 150 μL lysis buffer (1% Triton X100, 0.5% SDS, 1 tablet cOmplete EDTA-free protease inhibitor cocktail (Roche Diagnostics GmbH, Mannheim, Germany) in 10 mL PBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... and sequentially incubated for 1 hour in a serum-based blocking solution and then for 1 hour in blocking solution containing the anti-digoxigenin (Roche, 11093274910; 1/2000) or anti-Fluorescein (Roche ...
-
bioRxiv - Cancer Biology 2020Quote: ... 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol, supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail) for 10 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... The final pellet containing the chromatin fraction was resuspended in lysis buffer (10 mM HEPES, 500 mM NaCl, 1 mM EDTA, 1% NP-40, supplemented with protease and phosphatase inhibitor cocktails, Roche, and Benzonase, Millipore), sonicated 3 times at low amplitude ...
-
bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl, 5 mM EDTA, 0.5% SDS, 0.5% SDO, 1% Triton X-100, 1 mM PMSF, Roche cOmplete Mini Protease Inhibitors 1x), and homogenized (Precellys Evolution ...
-
bioRxiv - Biochemistry 2022Quote: ... The PVDF membrane was blocked with 5% skim milk in TBS for 1 h and incubated with anti-HA-HRP (Roche, 3F10, 1:5000) in 5% milk/TBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10% Glycerol, 1 % Triton-X-100, 1.5 mM MgCl2, 1 mM EGTA, 10 mM Pyrophosphate, 100 mM NaF, Roche protease inhibitor cocktail 1x), and protein level quantified using the Pierce BCA Assay Kit (Thermo Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... the cells were washed with cold 1X-PBS prior to collection with 800μL of chilled lysis buffer (50mM TRIS-HCl [pH=7.4]; 150mM NaCl; 1mM EDTA; 1mM CaCl2; 1mM MgCl2; 1% Triton, 0.5% sodium deoxycholate; protease inhibitor cocktail [Roche] 1 tablet/10mL lysis buffer). The cells were incubated on ice for 30 minutes and then centrifuged at 13,000 rpm/ 15minutes/ 4° C ...
-
bioRxiv - Neuroscience 2020Quote: ... 40 mM KCl, 5 mM EGTA, 5 mM MgCl2, 5 mM DTT, 1 mM PMSF, 1% Triton X, protease inhibitor Roche complete, pH 7.2) and sonicated at low power for 5 s ...
-
bioRxiv - Cancer Biology 2021Quote: ... 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol, supplemented with 100 U/mL SUPERase-IN and 1X Roche protease inhibitor cocktail) for 10 minutes ...
-
bioRxiv - Synthetic Biology 2021Quote: GST-tagged CBX8 was purified by re-suspending thawed cell pellets in 30 ml of lysis buffer (1× PBS, 5 mM DTT, 1× EDTA free protease inhibitor cocktail (Roche Diagnostics, Indianapolis, IN)) per liter of culture ...
-
bioRxiv - Biochemistry 2021Quote: ... The pellets of harvested bacteria were resuspended in 5 mL of lysis buffer (20mM NaP pH 7.5, 300mM NaCl, 15mM imidazole, 5% glycerol, 0.5mM TCEP, 1 mg/ml lysozyme, 5 U/ml DNase, 1 Roche protease inhibitor tablet/100mL) per 1 g of wet weight culture ...
-
bioRxiv - Biochemistry 2021Quote: ... The pellets were resuspended in 5 mL of lysis buffer (20mM NaP pH 7.5, 300mM NaCl, 15mM imidazole, 5% glycerol, 0.5mM TCEP, 1 mg/ml lysozyme, 5 U/ml DNase, 1 Roche protease inhibitor tablet/100mL) per 1 g of wet weight culture ...
-
bioRxiv - Microbiology 2020Quote: NUP153/NSP1-expressing cell pellets were resuspended in lysis buffer (1× PBS, 0.5 mM TCEP, 0.1 mM PMSF, 1× Roche cOmplete protease inhibitors, pH 7.4), and lysed in a homogenizer ...
-
bioRxiv - Genetics 2019Quote: ... Sheered chromatin samples were pre-cleared by incubating with the 250ul aliquot of Dynabeads resuspended in 2ml of Dilution Buffer (20mM Tris pH=8, 2mM EDTA, 150mM NaCl, 1% Triton X-100, 1:50 Roche cOmplete protease inhibitor) for 1 hour at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... 10 mM NaCl, 1.5 mM MgCl2, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) with 100 U/ml of OmniCleave nuclease (Epicentre ...
-
bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% Triton X-100, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail), incubated for 20 minutes on ice ...
-
bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% IGEPAL Nonidet P-40, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) and transferred to a Dounce homogenizer and homogenized with 20 slow strokes ...
-
bioRxiv - Cell Biology 2024Quote: ... Beads were spun down and washed twice in 1 mL of buffer (50 mM Hepes, pH 7.4, 150 mM NaCl, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001)) and subjected to sample preparation for MS measurement ...
-
bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche], and PhosStop [Roche]; pH 7.5) and sonicated for 30 s ...
-
bioRxiv - Neuroscience 2023Quote: ... The slices were lysed in lysis buffer (50 mM Tris/HCl, 1 mM EDTA, 1% SDS, phosphatase inhibitor cocktail (Roche, Indianapolis, IN, USA), and protease inhibitor cocktail (Roche) ...
-
bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 mL of buffer 1 (20 mM K-HEPES pH 7.9, 50 mM KCl, 10% glycerol, and Roche EDTA-free protease inhibitors). Subsequently ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... in 10 ml of extraction buffer (pH 6.7) (250 mM sucrose, 120 mM KCl, 10 mM MOPS, 5 mM MgCl2, 1 mM DTT, 1 Roche protease inhibitor cocktail tablet) (Vought et al ...