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Citations for Roche :
4701 - 4750 of 6468 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... They were then quantified by qPCR using the KAPA Library Quantification Kit (Kapa Biosystems, Roche, Basel, Switzerland) before amplification with Illumina’s cBot ...
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bioRxiv - Developmental Biology 2024Quote: ... the libraries were quantified by qPCR using the KAPA library quantification kit for Illumina platforms (Roche, KR0405) using QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... They were then quantified by qPCR using the KAPA Library Quantification Kit (Kapa Biosystems, Roche, Basel, Switzerland) before amplification with Illumina’s cBot ...
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bioRxiv - Immunology 2024Quote: Total RNA from T lymphocytes was isolated using the High Pure RNA isolation kit (Roche, catalog 11828665001), quantified in a NanoDrop (ND-1000 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Adapter-ligated fragments were quantified with qPCR using a library quantification kit for Illumina (KAPA Biosystems/Roche) on a CFX384Touch instrument (BioRad ...
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bioRxiv - Immunology 2024Quote: ... All libraries were quantified via Agilent 2100 hsDNA Bioanalyzer and KAPA library quantification kit (Roche, Cat. #0797014001). Libraries were sequenced at a targeted depth of 25,000 reads per cell using the NovaSeq 6000 S2 100 cycle kit (Illumina).
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bioRxiv - Plant Biology 2024Quote: ... The libraries were then quantified by qPCR using the KAPA Library Quantification Kit for Illumina Libraries (Roche), and their profiles were assessed on an Agilent Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then incubated overnight at 4°C in primary antibody dilutions in freshly prepared BBT+ buffer (PBST + 1% BSA + 0.5 mM Spermidine + 2 mM EDTA + 1 large Roche complete EDTA-free tablets). Primary antibody was replaced with BBT+ buffer and quickly washed twice ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Fixed and permeabilized embryos were incubated with DIG or Fluorescein-labeled RNA probes and the labeled probes were detected by Alkaline Phosphatase (AP)-conjugated primary antibodies (Roche Cat# 11093274910, RRID:AB_514497) and Roche Cat# 11426338910 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the primary antibodies diluted in blocking solution (H3K9me3: ab8898, 1:500, HP1α: CST #2616, 1:200, HA: Roche 3F10, 1:250) for 1h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... but with several differences from incubation with the anti-DIG antibody on: Incubation with blocking Buffer (2% Blocking Reagent from ROCHE in MABTween 1x) for 1 h ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen) and SOX9 (1:10000 ...
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bioRxiv - Cell Biology 2021Quote: ... along with the appropriate antibody (mouse HA 12CA5, 7.5 μl 0.4 mg/ml, Roche; mouse M2 FLAG, 5 μl 1 mg/ml, Sigma), before incubating at 4 °C on a rotating wheel at 14 rpm for 15-18 h ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were blocked in 10% BSA and 0.05% Tween 20 in PBS for 30 minutes before application of one of the following antibodies: mouse anti-human p16INK4a (CINTEC-Roche, cat# 705-4793), rabbit anti-human alpha-smooth muscle actin or aSMA (Abcam ...
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bioRxiv - Plant Biology 2021Quote: ... pH 7.4) for 30 min and subsequently incubated 1 h with anti-HA-peroxidase high-affinity monoclonal rat antibody (3F10; Roche [catalog no. 12013819001]) diluted 1:1000 in 2.5% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... We excluded those with evidence of previous COVID-19 (defined as a positive NAAT COVID-19 test or presence of anti-nucleocapsid antibodies in the blood [Roche, IND, USA] 7) given the known differential impact on antibody responses post-vaccination.8 We also excluded cases for whom the second vaccine was >130 days after the first ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was then incubated in antibody detection solution consisting of 1:20000 anti-HA rat mAb conjugated to horseradish peroxidase (HRP) (Roche [3F10], Cat. # 1201381900) diluted in MTBST 0.05% for 1 hour at room temperature on a tube rotator ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were washed and the secondary antibodies applied (dilutions listed below) with 4′-6-diamidino-2-phenylindole (DAPI: Cat # 10-236-276-001, Roche Diagnostics, Indianapolis, IN) at 1:1,000 for 1 hour ...
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bioRxiv - Physiology 2020Quote: ... and immunodetection of the DIG-labeled hybrids was performed with a peroxidase-conjugated anti-DIG antibody (1:2000; raised in sheep; Roche Diagnostics, Mannheim, GER). The fluorescence signal was developed with tyramide signal amplification (TSA ...
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bioRxiv - Neuroscience 2024Quote: ... Oocytes were then incubated for 60 min at 4 °C with 100 μg/ml rat monoclonal anti-HA antibody (clone 3F10, Roche Pharmaceuticals, Basel, Switzerland) in 1% BSA/ND96 ...
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bioRxiv - Cancer Biology 2024Quote: ... The sections were immediately covered with 20-60 µL primary antibody in Triton-Wash buffer (20mM HEPES pH 7.5,150mM NaCl, 2mM spermidine and Roche complete EDTA-free protease inhibitor) added dropwise ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then mounted to glass slides and coverslipped with Vectashield Vibrance (Vector H-1700) Primary antibodies used for immunohistochemistry (IHC) are listed here with dilutions indicated in parentheses: rat anti-HA (Roche 11867423001, 1:500), chicken anti-GFP (Abcam ab13970 1:1500) ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in anti-DIG POD (sheep anti-digoxigenin peroxidase conjugated antibody, RRID AB_514500; Roche, diluted 1:200 in the blocking buffer) overnight ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each labeled probe was sequentially detected by incubation with a peroxidase-conjugated antibody against digoxigenin and peroxidase-conjugated anti-fluorescein (Roche, Indianapolis, IN, USA) followed by incubation with TSA-Alexa Fluor 555 and TSA-Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated overnight at 4°C with anti-Digoxigenin-AP-conjugated antibody (dilution range: 1:1000 to 1:5000, Roche, catalog no. 11093274910) diluted in the same buffer ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse IgG1 mAb α-GFP (1:500, Roche Diagnostics #11814460001), mouse IgG2 mAb α-Centrin clone 20H5 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Libraries for April samples were prepared with the DNA Hyper Prep library kit (Kapa Biosystems-Roche, Basel, Switzerland) and libraries for August samples were prepared with the Nextera DNA Flex Library kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Libraries for April samples were prepared with the DNA Hyper Prep library kit (Kapa Biosystems-Roche, Basel, Switzerland) and libraries for August samples were prepared with the Nextera DNA Flex Library kit (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-seq libraries were generated using the Kapa mRNA-Hyper Prep kit with Poly-A Selection (Roche, Basal) and were sequenced on the Illumina NovaSeq (>70 million 2×150 bp sequences ...
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bioRxiv - Developmental Biology 2021Quote: ... Nuclear RNA-seq libraries were constructed with the Stranded RNA-seq Kit with Ribo Erase (Kapa Biosystems, KK8484) with custom Y-shaped adapters ...
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bioRxiv - Cell Biology 2020Quote: ... a DNA library was generated from 300 ng of genomic DNA using Kapa HyperPlus library preparation kit (Roche) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from asynchronous RPE-1 cells using the High Pure RNA Isolation kit (Roche, 11828665001) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Purified total RNA from pHCEnC was prepared for RNA sequencing libraries using the KAPA mRNA HyperPrep Kit (Roche Sequencing Solutions ...
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bioRxiv - Immunology 2021Quote: ... Each cDNA sample was ten times diluted and amplified using KAPA SYBR® FAST qPCR Kit (Kapa Biosystems) on the CFX Connect™ Real-Time PCR detection System (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: The presence of lactate dehydrogenase (LDH) in cell culture supernatants was measured using the Cytotoxicity Detection kit (Roche), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... RNA-seq libraries were prepared using the KAPA Stranded mRNA-seq Kit with mRNA capture beads (KAPA Biosystems) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... and the fragmented DNA was input for library construction by the KAPA Hyper Preparation Kit (Kapa Biosystems, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Sense and antisense RNA probes were transcribed from linearized plasmid DNA using a DIG RNA Labeling Kit (Roche). Sections were prepared by embedding hybridized embryos in gelatin-albumin and sectioning on a vibratome (Leica VT1000S ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The RNASeq libraries were prepared from total RNA using the KAPA Stranded mRNA-Seq Kit for Illumina (Roche) with minor modifications ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmid DNA was extracted from wild-type strains using Genopure Plasmid Midi Kit (Roche Diagnostics GmbH, Mannheim, Germany). Plasmids were reintroduced by electroporation and their presence was confirmed by PCR assays for gene blaCTX-M and for FII and multiplex (FIA ...
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bioRxiv - Microbiology 2019Quote: ... and was reverse-transcribed to cDNA using Transcriptor First Strand cDNA Synthesis Kit with random primers (Roche, 04897030001). The relative abundance of HTLV-1 sense-strand transcripts at each time point was measured by qPCR with Fast SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The libraries were quantified by using the KAPA library quantification kit for Illumina sequencing platforms (KAPA Biosystems, KK4824) and sequenced on HiSeq following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2020Quote: ... and then diluted for accurate qPCR quantification using KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, MA). The sequencing pool was adjusted to a final concentration of 11.5 pM (for V2 kits ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 ng or less of DNA was used for library preparation using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... prior to the preparation of an adapter-ligated library using the KAPA JyperPlus Kit (KAPA Biosystems, Wilmington, MA) according to KAPA-provided protocol ...
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bioRxiv - Microbiology 2020Quote: Real time quantitative RT-PCR was performed with the Light Cycler RNA Master SYBR Green I kit (Roche), following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: mRNA was extracted at the indicated time points using the High Pure RNA Isolation kit (Roche, Mannheim, Germany). cDNA was generated using M-MuLV reverse transcriptase (NEB ...
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bioRxiv - Genomics 2019Quote: ... 2015) that uses the tube assemblies from the High Pure Viral Nucleic Acid Large Volume kit (Roche, 05114403001). The intact ossicles were placed in the extraction buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... The purified ChiP DNA was used to generate sequencing libraries using Hapa Hyper prep kit from Kapa Biosystems. Libraries were sequenced in single-end using Illumina Hiseq 2500 or Illumina Hi-seq 4000 at 50 cycles.
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bioRxiv - Microbiology 2019Quote: Transcriptional start sites of the virD and virB transcripts were obtained by using the 5’ RACE Kit (Roche), as described (Andrade et al ...