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Citations for Roche :
4451 - 4500 of 5370 citations for QuantiFluo Ammonia Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed using the LightCycler 480 Probe Master Kit and the LightCycler 480 II system (Roche Applied Science) as described previously92 ...
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bioRxiv - Microbiology 2021Quote: ... Radiolabeled probe was generated by purifying a digested (Ncol; 1 cut) pCpG vector DNA using High Pure PCR product Purification Kit (Roche) followed by radiolabeling the probe using Random Prime DNA labeling kit (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... Illumina compatible Indexed libraries were prepared from 12ng of Diagenode Biorupter Pico sheared ChIP DNA using the KAPA Hyper Library Preparation Kit (Roche). Libraries were enriched with 2 cycles of PCR then assessed for size distribution using the 4200 TapeStation High Sensitivity D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... 1μl of cDNA diluted with 10-fold MQ was mixed with KAPA SYBR Fast qPCR kit and amplified with Lightcycler 96 (Roche; 95℃ 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Triplicate reactions that amplified were pooled together and quantified with Kapa library quantification kit (Kapa Biosystems, KK4824, Wilmington, MA, USA) before equimolar sample mixing ...
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bioRxiv - Immunology 2020Quote: ... The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Equimolar amounts of each library were sequenced on a NextSeq 500 instrument controlled by the NextSeq Control Software (NCS ...
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bioRxiv - Immunology 2020Quote: ... Amplifications were carried out on a LightCycler 480 II instrument using LightCycler 480 software (v. 1.5.0) and a LightCycler 480 SYBR green I master kit (Roche Applied Science) according to the provided instructions ...
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bioRxiv - Immunology 2021Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Immunology 2021Quote: ... cell nuclei with DNA strand breaks at the early stage of apoptosis were labeled using fluorescein-dUTP in the TUNEL reaction (In Situ Cell Death Detection Kit; Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from homogenized tissue of adenovirus vectored vaccine inoculated mice by High Pure Viral Nucleic Acid Kit (Roche). DNA fragments of Sad23L and Ad49L vectors were amplified by nested PCR with primers specific to adenoviral hexon sequences (Table S4 ...
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bioRxiv - Immunology 2021Quote: ... Poly A mRNA was purified from total RNA using oligodT magnetic beads and strand-specific indexed libraries were prepared using the Illumina’s TruSeq Stranded mRNA Sample Prep Kit followed by ten cycles of amplification using KAPA HiFi DNA polymerase (KAPA Biosystems). Libraries were quantified and pooled based on a post-PCR Bioanalyzer and 75 base pair (bp ...
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bioRxiv - Immunology 2021Quote: ... Synthesized DNA specific to regions of homologous recombination (see Oligonuleotide section in Key Resources Table) was labeled with 32P (Amersham) using the High Prime labeling kit (Roche) according to manufacturer’s instructions and unincorporated 32P was removed with G-50 Sephadex Quick Spin columns (Fisher) ...
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bioRxiv - Immunology 2021Quote: Sample RNA extraction was performed from freshly isolated C57BL/6J WT pMBEMCs following the manufacturers instruction of the High Pure RNA Isolation Kit (Hoffman-La Roche). cDNA was obtained from total isolated RNA of each sample with the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... RNA mRNA libraries of template molecules suitable for high throughput DNA sequencing were created using the KAPA mRNA HyperPrep Kit (Roche). Briefly ...
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bioRxiv - Genomics 2020Quote: ... Quantification and quality control of the final HiChIP library was conducted using a Bioanalyzer and KAPA quantification kit (Kapa Biosystems). Libraries underwent Next Generation Sequencing on a HiSeq 2500 generating 100 bp paired-ends.
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bioRxiv - Neuroscience 2021Quote: ... Alkaline hydrolysis was then performed at 60°C for 30 min to fragment the target RNA before labeling it with Digoxigenin and Fluorescein according to kit instructions (Roche).
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bioRxiv - Microbiology 2021Quote: ... Library concentration for pooling of barcoded samples was assessed by RT-qPCR with a KAPA Library Quantification kit (KAPA Biosystems) as recommended for high sensitivity ...
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bioRxiv - Immunology 2022Quote: ... and 11-week infected half brains and liver lobes of mice (n=4/group) was extracted and purified using a High Pure PCR Template Prep Kit (Roche). DNA concentration of each sample was determined via NanoDrop ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA was enriched by poly-A capturing and RNA-seq libraries were generated using KAPA mRNA HyperPrep Kit (KAPA Biosystems).Libraries were sequenced using the Illumina HiSeq 1500 platform.
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bioRxiv - Cancer Biology 2022Quote: ... Genomic DNA contamination was removed by DNase I treatment of the isolated RNA using DNase I RNase free kit (Roche). After quantification of the RNA and quality check ...
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bioRxiv - Cell Biology 2022Quote: Following library construction as per manufacturer’s instructions ST libraries were quantified using the KAPA-Illumina PCR quantification kit (KAPA Biosystems) and pooled at 4nM concentration with a sample ratio corresponding to the surface area of tissue coverage obtained from the H&E imaging ...
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bioRxiv - Neuroscience 2022Quote: cDNA was generated from TRAP-isolated and total input RNA samples (n=2 samples/group) using the Transcriptor First Strand cDNA Synthesis Kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by using 1:500 diluted rabbit polyclonal antibodies against ferroportin (49) and an appropriate detection kit (Omnimap rabbit polyclonal HRP, #760-4311 and ChromoMap-DAB #760-159; Roche). Negative controls were performed by the omission of the primary antibody ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted from the tail tip using the KAPA Express Extract DNA Extraction Kit (Kapa Biosystems, London, UK) and Animal Tissue Direct PCR Amplification Kit (with TL ...
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bioRxiv - Molecular Biology 2022Quote: ... The blots were hybridized with the MVC VP2 genome probe using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Probe labelling and DNA hybridization were performed following the protocol provided with the PCR-DIG DNA-labelling and chemiluminescent detection kit (Roche).
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bioRxiv - Cancer Biology 2022Quote: 100 to 500 ng of total RNAs were reverse-transcribed with Transcriptor First Strand cDNA synthesis kit (Roche, Basal, Switzerland). qPCR experiments were performed using the StepOnePlus qPCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... according to the manufacturer’s instruction and reverse-transcribed with the aid of Transcriptor First Strand cDNA synthesis kit (Roche, Switzerland). qPCR experiments were performed using the StepOnePlus qPCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: The activity of telomerase in the cell lines was detected using the TeloTAGGG telomerase PCR ELISA Kit (Roche, Cat# 12013789001). The assay was performed according to the manufacturer’s instructions and repeated in triplicate ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-Seq library was prepared according to KAPA mRNA HyperPrep kit with 200-300 bp insert size (Roche, Wilmington, MA) using 250 ng of total RNAs as input ...
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bioRxiv - Cancer Biology 2022Quote: ... Validation of the library was performed on Fragment Analyzer (Standard Sensitivity NGS kit) and using qPCR (ROCHE Light Cycler480, France). Total RNAs were sequenced on NovaSeq 6000 from Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Digoxigenin (DIG)-labeled RNA probes were transcribed in vitro using a DIG RNA labeling kit (Roche Applied Science, Basel, Switzerland) and brain sections were hybridized with gene-specific antisense RNA probes ...
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bioRxiv - Cancer Biology 2022Quote: ... and 500 ng of DNA-free RNA was reverse transcribed using the First Strand cDNA Transcriptor Synthesis Kit (Roche Life Science, Roche Diagnostics Division ...
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bioRxiv - Cancer Biology 2022Quote: ... the DNA fragments were “End-Repaired,” “A-tailed,” and the index adapters were ligated (Agencourt AMPure XP Beads; SeqCap EZ Pure Capture Bead Kit; Hoffmann-La Roche). Following that ...
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bioRxiv - Developmental Biology 2022Quote: 155BS-upd from Doug Harrison and RE35237 from BDGP cDNAs were used to generate upd and sna RNA probes using the DIG RNA Labeling Kit (Roche). Secondary biotinylated antibody against mouse (1:200 ...
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bioRxiv - Genomics 2022Quote: ... RNA Libraries were prepared from 100 ng of total RNA with the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems KK8401). Indexed libraries were then pooled and 2×50 bp ...
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bioRxiv - Genomics 2022Quote: ... and P5 and P7 adapters were ligated per pool following the KAPA HiFi Hotstart PCR kit standard protocol (Kapa Biosystems). Pooled samples were then combined into one sample and cleaned as before ...
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bioRxiv - Plant Biology 2022Quote: ... Sense and anti-sense digoxigenin-labelled probes were synthesised using the DIG RNA Labeling Kit (SP6/T7; Roche, Basel, Switzerland) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the ligated product was amplified by 12 PCR cycles using the Kapa Hifi Hotstart NGS library amplification kit (Kapa Biosystems), followed by purification with 0.6×AMPure XP ...
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bioRxiv - Plant Biology 2022Quote: ... and 72°C for 20 s) containing the Spartina cDNAs with the PCR DIG Probe Synthesis Kit (Roche, Cat.11636090910) using the SaHKT1 transcripts specific primers (Forward primer ...
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bioRxiv - Plant Biology 2022Quote: ... The RNA level was detected using the DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Cat. 11585614910).
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bioRxiv - Microbiology 2022Quote: ... was collected from infected organoids for HBeAg and HBsAg quantification using the Elecsys HBeAg and HBsAg II kit chemiluminescent microparticle immunoassay (CMIA) on the Cobas e602 instrument (Roche).
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... The antisense RNA probes corresponding to the 3’ s-end portion of glnA CDS were prepared by the DIG RNA Labeling Kit (Roche).
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bioRxiv - Plant Biology 2022Quote: ... derived from SlARF2A and SlARF2B promoter was labeled with digoxigenin as per the manufacturer’s protocol using DIG Oligonucleotide 3′-End Labeling Kit (Roche Diagnostics). The same unlabeled DNA fragment was used as a competitor ...
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bioRxiv - Plant Biology 2022Quote: ... derived from SlGLYI4 promoter was labelled with digoxigenin as per the manufacturer’s protocol using DIG Oligonucleotide 3′- End Labelling Kit (Roche Diagnostics). The same unlabelled DNA fragment was used as a competitor ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). Primers used in the RT-qPCR are listed in Supplementary Table S1 ...
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bioRxiv - Neuroscience 2023Quote: ... 2008) using commercial transcription kits and RNA labeling mix (REF #: 11277073910, Lot #: 26591021 & REF #11426346910:, Lot # 24158720; Roche Applied Science) to generate digoxigenin-labeled and fluorescein-labeled riboprobes using a plasmid template containing cDNA ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Ten million cells were then lysed with a Dounce homogenizer on ice in the kit lysis buffer supplemented with EDTA-free protease inhibitors (Roche). Cell homogenates were subjected to immunoprecipitation using magnetic beads-coupled anti-TOMM22 antibodies ...
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bioRxiv - Plant Biology 2023Quote: The YFP digoxygenin-labelled antisense and sense RNA probes were generated by in vitro transcription according to the instructions provided with the DIG RNA labelling kit (SP6/T7; Roche) using a plasmid containing the YFP sequence as template ...