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Citations for Roche :
401 - 450 of 3664 citations for hsa mir 222 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 100 µM random hexamer primers (Roche # 11034731001) and 1 μl RNAsin Plus Inhibitor (Promega #N2615 ...
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bioRxiv - Microbiology 2023Quote: ... using random primers p(dN)6 (Roche). Quantitative real-time PCR was performed on a real-time PCR system (Quant Studio 6 Flex ...
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bioRxiv - Cell Biology 2024Quote: ... using random primers p(dN)6 (Roche). Quantitative real-time PCR reactions employing SYBRgreen fluorescent reagent and/or EvaGreen™ were performed in the Stratagene Mx3000P system (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... and 2.55 nL of 72 Ad153-reverse-tag (1~72) primer [1 μM] made by KAPA HiFi HotStart PCR Kit (KK2500, Kapa Biosystems, Cape Town, South Africa). Finally ...
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bioRxiv - Genetics 2020Quote: ... A LYS2 probe was amplified and labeled with Digoxigenin (DIG)-dUTP using the primers 5’-TGAAGCCTTCCCAGAGAGAA and 5’-GCCAAGGAAAAATGTCTACCA (Roche PCR DIG Probe Synthesis Kit). The DIG probe was hybridized to the membrane (at 44°C ...
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bioRxiv - Microbiology 2020Quote: ... Thermocycling conditions were set on a LightCycler® 96 Instrument (Roche), as follows ...
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bioRxiv - Molecular Biology 2019Quote: ... and ligated to KAPA single indexed adapters Set B (KK8702, Roche). Samples were processed according to manufacturer’s instructions with one exception ...
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bioRxiv - Developmental Biology 2020Quote: ... Reaction was set up with KAPA SYBR® FAST Universal (Roche) in a total volume of 10 µl ...
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bioRxiv - Microbiology 2021Quote: ... and detected with DIG wash and block buffer set (Roche-11585762001).
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bioRxiv - Bioengineering 2023Quote: ... of the VH gene was subjected to second-strand synthesis in a 25 μl reaction volume using IGHV gene-specific primers (primer No. 23–32, 7.5 μl) and a KAPA Biosystems kit (Roche). The reaction conditions were as follows ...
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bioRxiv - Microbiology 2023Quote: ... where SARS-CoV-2 RNA was detected by real time reverse transcriptase real time polymerase chain reaction (RT-PCR) one either a Cobas® 6800 (Roche Diagnostics GmbH (Mannheim, Germany)) ...
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bioRxiv - Cell Biology 2019Quote: ... 4 μl 5xTranscriptor RT reaction buffer (Roche), 0.5 μl RNase OUT (Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR reactions on a LightCycler480 (Roche) to probe for the genes of interest (see Supplementary Table 1 for primer sequences) ...
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bioRxiv - Microbiology 2023Quote: ... no-chloroplast amplifying primers 335F (CADACTCCTACGGGAGGC) and 769R (ATCCTGTTTGMTMCCCVCRC) (Dorn-In et al. 2015) were used together with KAPA2G Robust PCR Kits (Kapa Biosystems, Cat. No. KK5005, Wilmington, USA). In protocol 2 (P2) ...
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bioRxiv - Developmental Biology 2019Quote: ... 5 pmol of the forward and reverse gene-specific primers each (primer sequences are shown in Table S3) in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 pmol of the forward and reverse gene-specific primers each (primer sequences shown in table S2) in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... Probe was prepared by PCR amplification using forward primers LL/F 5′-TACGGACACAGGTCGAATCCCCTACTACC-3′ and reverse primer LL/R 5′-ACAGAGAAGAGGCTAATGTGTGCAC-3′ in the presence of DIG (Roche). PCR-products were resolved in a 1.2 % agarose Ethidium bromide-stained gel ...
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bioRxiv - Genomics 2023Quote: ... 100ng of library were amplified using an upstream (US) forward primer and a universal reverse primer using the KAPA2G Robust HotStart ReadyMix (Roche). The resulting product was amplified using a downstream (DS ...
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bioRxiv - Cancer Biology 2019Quote: ... and specific primers on a LightCycler 480 (Roche). The reactions were performed in triplicates ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized using random primers (Roche, #11034731001). Samples were amplified for 35 cycles using the 7500 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was produced with random hexameric primers (Roche) using M-MLV (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... All primers that were used introduced EcoRI (Roche) and BamHI (Roche ...
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bioRxiv - Physiology 2021Quote: ... with primers detailed below on LightCycler480 apparatus (Roche). Gene expression levels were normalized with hypoxanthine phospho-ribosyltransferase (HPRT) ...
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bioRxiv - Immunology 2020Quote: ... 0.5 μg PolyT primer for cDNA synthesis (Roche) and RNasin inhibitor (Promega) ...
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bioRxiv - Immunology 2021Quote: ... Primer sequences and corresponding probes (Metabion, Roche & TipMolBIOL) are listed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and specific primers on the LightCycler 96 (Roche). Relative target gene expression was determined by comparing average threshold cycles (CT ...
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bioRxiv - Cancer Biology 2023Quote: ... with primers detailed below on LightCycler480 apparatus (Roche). Gene expression levels were normalized with hypoxanthine phospho-ribosyltransferase (Hprt) ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene-specific primers and probes (Universal ProbeLibrary, Roche) were used in combination with qPCR Probe-MasterMix (SL-9802 ...
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bioRxiv - Cancer Biology 2024Quote: ... and specific primers on the LightCycler 96 (Roche). Relative target gene expression was determined by comparing average threshold cycles (CT ...
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bioRxiv - Developmental Biology 2020Quote: ... The library pool was hybridized with a custom Nimblegen probe set (Roche), targeting protein-coding regions and non-coding RNAs (lncRNAs and miRNAs) ...
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bioRxiv - Genetics 2021Quote: ... and indexed with KAPA Single-Indexed Adapter Set A + B (Kapa Biosystems). Library quantification was by Qubit 1x dsDNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... and hybridization probes from the Universal Probe Library Set (Roche Life Sciences). The relative expression ratio of target/reference gene was calculated with the ΔΔCt method (Pfaffl 2001) ...
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bioRxiv - Neuroscience 2021Quote: ... The sequences of the specific forward and reverse primer pairs were constructed using the Primer-BLAST tool or using the “Universal Probe Library” software (Roche Diagnostics). Sequences of the different primer pairs used are listed in Supplementary Table S1 for humans and Supplementary Table S2 for rats ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was synthesized from total RNA with anchored-oligo(dT)18 primer and random hexamer primer using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany). The template-primer mix was denaturized at 65 °C ...
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bioRxiv - Genetics 2020Quote: ... cDNA was synthesised from the total RNA with anchored-oligo(dT)18 primer and random hexamer primer using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany). One μg of RNA was added to each 20 μl RT reaction ...
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bioRxiv - Cancer Biology 2019Quote: Reverse transcription (RT) reactions were carried out using 1 μg of RNA using Transcriptor RT enzyme (Catalog No. 03531287001) from Roche Diagnostics ...
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bioRxiv - Molecular Biology 2019Quote: ... avian myeloblastosis virus reverse transcriptase (AMV RT; Roche) were used to synthesize cDNA from total RNA (2.5 µg ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was performed on a LightCycler480 (Roche) in a 10-μL reaction mixture containing 8 ng of cDNA ...
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bioRxiv - Neuroscience 2019Quote: ... RT-qPCR was run using SYBR green (Roche) on a QuantStudio 6 (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using SYBR Green (Roche) and a QuantStudio 7 Flex (Life Technology ...
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bioRxiv - Plant Biology 2019Quote: ... Using RTS 100 Wheat Germ CECF Kit (Roche), GUN1 protein was expressed in RTS ProteoMaster (Roche) ...
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bioRxiv - Plant Biology 2019Quote: ... GUN1 protein was expressed in RTS ProteoMaster (Roche). Full-length GUN1 cDNA and N-terminal truncated versions as EcoRI-SalI PCR fragments were ligated into pET48b (Novagen ...
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bioRxiv - Cell Biology 2022Quote: ... anti-HA (rt, 1:1000, Roche, clone 3F10), anti-CNG channel (ms ...
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bioRxiv - Cancer Biology 2023Quote: ... RT-qPCR was run on Lightcycler 480 (Roche) using SYBR Green 1 Master Kit (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... or RevertAid RT (Thermo) with random hexamers (Roche). qPCR was performed with the SensiFAST No-ROX Probe Master Mix (Bioline ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR experiments were performed with primers designed via the Primer-BLAST tool on the NCBI website and SYBR-Green (Roche, Cat#: 04707516001) in a Roche LightCycler 480 Instrument II to quantify the results ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were blocked in blocking solution (Roche DIG wash and block buffer set) diluted in 1x maleic acid for 1h at RT and riboprobes were detected by incubating in anti-digoxigenin-AP antibody (1:2000 in 1% sheep serum ...
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bioRxiv - Microbiology 2021Quote: ... Temperature cycling conditions were set on a LightCycler® 480 Instrument II (Roche), using the following parameters ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR reactions were set up using FastStart Essential DNA Green Master (Roche, #06402712001) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: ... Transcription reactions were set up to label probes with digoxigenin (DIG, # 11277073910, Roche) and incubated at 37°C for two hours ...