Labshake search
Citations for Roche :
401 - 450 of 1350 citations for Recombinant Human CD19 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... DIG-labeled sense and antisense RNA probes were synthesized with T7 RNA polymerase (Roche). For immunological detection ...
-
bioRxiv - Cell Biology 2021Quote: ... using a digoxigenin-labeled probe and an anti-dig-HRP antibody (Roche Applied Science). In two-color FISH ...
-
bioRxiv - Molecular Biology 2022Quote: Probes were labeled according to the Nick Translation-based DNA Probe Labeling method (Roche). Tak-1 and Tak-2 genomic DNA was extracted by CTAB method (Murray & Thompson ...
-
bioRxiv - Molecular Biology 2022Quote: ... DIG-labeled probes were prepared using the DIG Oligonucleotide tailing kit (2nd generation; Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: DIG-labeled DNA probes were synthesized using PCR DIG Probe Synthesis Kit (Roche #11636090910). Primers were designed to target either external (676 bp ...
-
bioRxiv - Neuroscience 2020Quote: ... and detection of digoxigenin-labeled probes were carried out according to manufacturer’s protocol (Roche), using anti-digoxigenin antibody conjugated to alkaline phosphatase for detection ...
-
bioRxiv - Molecular Biology 2022Quote: ... and CAT5/COQ7 were labeled with digoxigenin using DIG Northern Starter Kit (Roche Diagnostics).
-
bioRxiv - Evolutionary Biology 2022Quote: ... DIG labeled riboprobes localization was detected by immunostaining with anti-DIG-Fab-AP (ROCHE) and NBT/BCIP.
-
bioRxiv - Molecular Biology 2019Quote: ... and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche; 1:100 dilution). Samples were mounted using SlowFade Diamond Antifade with DAPI mounting media (Invitrogen ...
-
bioRxiv - Molecular Biology 2019Quote: ... labeled with alkaline phosphatase–conjugated sheep anti-DIG FAB fragments (11093274910, Roche, Basel, Switzerland), before visualized with Liquid Permanent Red (DAKO ...
-
bioRxiv - Plant Biology 2020Quote: ... Digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen) ...
-
bioRxiv - Developmental Biology 2020Quote: ... and probes labeled with digoxigenin-11-dUTP were detected using anti-digoxigenin rhodamine (Roche). The preparations were counterstained using DAPI and mounted in Vectashield ...
-
bioRxiv - Neuroscience 2019Quote: ... or IMPA1L 120nt fragment were labeled using Random Priming Labeling Kits (Takara or Roche) and [α−32P] dCTP ...
-
bioRxiv - Neuroscience 2019Quote: ... Antisense digoxigenin (DIG)-labeled RNA probes were synthesized using DIG RNA Labeling Mix (Roche). Human and mouse ALDH1A3 (Clone ID 6208628 ...
-
bioRxiv - Genetics 2019Quote: ... The incorporated BrdU was then detected using a FITC-labeled anti-BrdU antibody (Roche). Coverslips were mounted with Prolong Gold antifade plus DAPI (Invitrogen) ...
-
bioRxiv - Microbiology 2019Quote: ... Nylon membranes were cross-linked and used for hybridization with DIG-labeled probe (Roche). Hybridization was done overnight at 65°C using hybridization solution (FastHyb-Hybridization solution ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... DIG labeled probes were made using SP6 or T7 transcription kits (Roche, Mannheim, Germany) from linearized plasmid or ...
-
bioRxiv - Microbiology 2020Quote: ... All strains generated were verified by Southern blot analysis using digoxygenin labeled probes (Roche). For ip insertions ...
-
bioRxiv - Genomics 2021Quote: ... DIG-labeled LINE DNA probe was prepared by PCR DIG Probe synthesis kit (Roche). Hybridization and autoradiography were performed according to the DIG Application Manual (Roche).
-
bioRxiv - Plant Biology 2022Quote: ... and digoxigenin-labeled using a DIG oligonucleotide 3’end labeling kit (Roche Applied Science). Ten picomoles of each probe were used for each slide ...
-
bioRxiv - Cell Biology 2023Quote: ... uuclei were labeled with Hoechst dye and 0.2 U/μl Protector RNase inhibitor (Roche). All GFP+ Hoechst stained myonuclei were purified using FACS (BD Aria ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were then incubated in alkaline phosphatase-labeled anti-DIG antibody (Roche; 1:600) in TNB for 2 h ...
-
bioRxiv - Cell Biology 2023Quote: ... Nuclei were labeled with Hoechst dye and 0.2 U/μl Protector RNase inhibitor (Roche). The labeled nuclei were purified using FACS (BD Aria ...
-
bioRxiv - Developmental Biology 2022Quote: ... and digoxigenin-labeled using a DIG oligonucleotide 3′ end labeling kit (Roche Applied Science). 10 picomols of each probe were used for each slide ...
-
bioRxiv - Plant Biology 2023Quote: ... and digoxigenin-labeled using a DIG oligonucleotide 3′ end labeling kit (Roche Applied Science). Ten picomoles of each probe were used for each slide ...
-
bioRxiv - Plant Biology 2023Quote: ... Digoxigenin-labeled RNA probes were synthesized using a DIG northern Starter Kit (Roche, 11277073910), according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... and hybridized with DIG-labeled probe using DIG Easy Hyb (Roche Diagnostics, Mannheim, Germany). Salmon sperm DNA was used in the blocking step ...
-
bioRxiv - Plant Biology 2023Quote: ... Digoxigenin-labeled probes were detected with anti-digoxigenin coupled to fluorescein isothiocyanate (FITC; Roche). Chromosome preparations were counter-stained with 4′,6-Diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Developmental Biology 2023Quote: ... In vitro transcription was performed using a T7 polymerase and DIG-labeled nucleotides (Roche). RNAscope was performed on sectioned zebrafish larvae as previously described (Carroll ...
-
bioRxiv - Neuroscience 2024Quote: ... and fluorescein (FITC)-labeled in vitro transcription of rln3a and sst1.1 probes (11685619910, Roche). The gsc2 ...
-
bioRxiv - Genetics 2024Quote: ... The incorporated BrdU was then detected using a FITC-labeled anti-BrdU antibody (Roche). Coverslips were mounted with Prolong Gold antifade plus DAPI (Invitrogen) ...
-
bioRxiv - Genetics 2020Quote: N-terminal HA-tagged PRUNE1 overexpression constructs were transfected into PRUNE1 non-expressing HEK293 cells using X-tremeGENE HP DNA (Roche; 06366236001) transfection reagent ...
-
bioRxiv - Immunology 2022Quote: ... filtered conditioned medium was loaded onto a 5 ml cOmplete™ His-tag Purification Column (Roche Diagnostics, Vilvoorde, Belgium). The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
-
bioRxiv - Biophysics 2020Quote: ... The supernatant was clarified by a 5 µm filter followed by a 0.45 µm filter and loaded on a cOmplete His-Tag purification Column (Roche) with a column volume (CV ...
-
bioRxiv - Biophysics 2020Quote: ... the pool was loaded in 0.5 mL GST resin mixed with 1 mL of cOmplete His-tag purification resin (Roche) stacked in a 12 mL polyprep column ...
-
bioRxiv - Molecular Biology 2019Quote: ... Purified products were subjected to second strand synthesis using 0.3 µM of second strand biotinylated primer and the KAPA Hi-Fi hot start ready mix (KK2601, Roche). The second strand reaction was carried out at 95 °C for 3 minutes ...
-
bioRxiv - Genomics 2020Quote: ... The quality and quantity of the capture Hi-C libraries was tested by Bioanalyzer and KAPA qPCR (Kapa Biosystems). Capture Hi-C libraries were analysed by 75 bp paired-end Next Generation Sequencing on an Illumina NextSeq500 (My-La ...
-
bioRxiv - Biochemistry 2022Quote: ... Clarified supernatant was filtered through a 0.45 μM PES syringe filter and incubated with 10 mL of cOmplete His-Tag purification beads (Roche) for 1 h with gentle rocking ...
-
bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... then subject to second-strand synthesis using 1.5 μl biotinylated oligo (10 μM) and 25 μl KAPA Hi-Fi hot start ready mix 2x (KK2601, Roche). Second strand reactions were incubated at 95°C for 3 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... 400 μl sonication solution and 3.8 μg/μl of proteinase K (proK, Recombinant PCR Grade [Roche]) for fraction B2 ...
-
bioRxiv - Microbiology 2022Quote: DNase treatment was performed according to the manufacturer’s protocols (RNase-free DNase I recombinant, Roche, US) for 1 hour ...
-
bioRxiv - Genetics 2021Quote: ... all RNA samples used in this study were treated with recombinant RNase-free DNase I (Roche) (10 U of DNase I per 3 mg of total RNA ...
-
bioRxiv - Microbiology 2020Quote: ... the recombinant RBD007 plasmid was transfected using FuGENE 6 transfection reagents (Roche Applied Science, Indianapolis, IN) into CHO-K1 cells precultured in F-12K medium (American Type Culture Collection ...
-
bioRxiv - Cell Biology 2020Quote: ... Chloroform extracted RNA was treated with RNase free recombinant DNase I (047 716 728 001, Roche) for 1 h at 37°C using 2 units/μg of RNA ...
-
bioRxiv - Bioengineering 2023Quote: ... coli DH10B recombinant strain was extracted using the High Pure Plasmid Purification Kit (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... The embryos were incubated in 50 μL of recombinant Proteinase K (Roche Holding AG, Basel, Switzerland) diluted to ∼2 mg/mL in 1 x tris-EDTA (TE ...
-
bioRxiv - Cancer Biology 2021Quote: Total cell lysates from human RMS cell lines and human myoblasts were obtained following lysis in RIPA lysis buffer supplemented with protease inhibitors (Roche). Western blot analysis was performed similar to Ignatius et ...