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Citations for Roche :
401 - 450 of 801 citations for Recombinant Human BDH2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by standard SDS-PAGE and transferred to PVDF membranes (Roche) using a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... Digestion of proteins was carried out by addition of trypsin (proteomics grade, Roche) at a 1/50 enzyme/protein ratio (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... Urine was analyzed for Protein and Albumin (Cobas c 311 Analyzer; Roche Diagnostics).
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bioRxiv - Molecular Biology 2023Quote: Predigested proteins and peptides were reduced with 10 mM dithiothreitol (DTT) (Roche, 10197777001) for 30 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... 80-100 μL of a mix of protein A and G-Agarose (Roche) was added to the samples ...
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bioRxiv - Neuroscience 2022Quote: ... immunoprecipitated using 50µl packed Protein G beads bound with anti-GFP antibodies (Roche), and in vitro kinase assay was performed on these samples as described above ...
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bioRxiv - Biochemistry 2023Quote: ... The protein on the membrane were probed with antibodies (either anti-HA (Roche),-FLAG (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... GFP- or HA3x-tagged proteins were detected using α-GFP (1:1000; Roche) or α-HA3x (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by a tryptic digestion (Roche, enzyme to protein ratio 1:50, MSgrade) and incubation at 37°C overnight in a thermal shaker at 700 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... 1 % Brij97 (v/v) and phosphatase and proteins inhibitors (Roche, # 4906837001 and # 4693132001) for 30 min under end over end rotation at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: Total protein extracts were prepared in RIPA buffer with protease inhibitor cocktail (Roche). Samples were sonicated for 3 minutes alterning 30seconds ON/ 30seconds OFF ...
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bioRxiv - Cell Biology 2022Quote: Conjugation of protein or nanobody was performed with an 8-fold (Laminin; Roche) or 4-fold (R2-myc-his ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were used for immunoblot with anti-GFP (Roche #11814460001, 1:1,000), anti-RFP (Chromotek #6g6 ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes (175 mg protein) were treated with one cOmplete protease inhibitor tablet (Roche) then solubilised on ice at 4.5 mg mL−1 by the stepwise addition of lauryl maltose neopentyl glycol (LMNG ...
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bioRxiv - Immunology 2023Quote: ... After adding 50[μl of 50% of Protein-G bead (Roche Applied Science) to lysates and incubation with rotation at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were electrophoretically transferred overnight on to PVDF membrane (03010040001, Roche, Sigma-Aldrich). The membrane was blocked in 5% non-fat dry milk in TBS with 0.05% Tween 20 (TBST ...
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bioRxiv - Neuroscience 2024Quote: ... Each resulting denatured protein sample was chemically reduced with 100 mM dithiothreitol (Roche) at 56°C for 30 min and alkylated using 100 mM iodoacetamide for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... kidney (human primary renal proximal tubule epithelial cells, RPTEC) and liver (human hepatocellular carcinoma, HepG2) cells were assessed using a standard WST-1 (Roche) cell viability assay ...
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bioRxiv - Microbiology 2020Quote: Damage to the human colon epithelial cell line HT-29 was assessed using a lactate dehydrogenase (LDH) cytotoxicity detection kitPLUS (Roche), which measures the release of the LDH enzyme in the growth medium ...
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bioRxiv - Cell Biology 2020Quote: ... MEFs were seeded subconfluently o/n (unless indicated otherwise) onto coverslips acid-washed and coated with human fibronectin (25 μg/ml, #11051407001, Roche), as described (Dimchev and Rottner ...
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bioRxiv - Cancer Biology 2022Quote: ... All embryonic histology slides and human tissue microarray (BioMax, U.S. #BC001134b) slides were scanned using a Ventana DP200 slide scanning system (Roche Diagnostics) at 20x magnification ...
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bioRxiv - Biochemistry 2019Quote: ... and S278E amino acid substitutions were introduced into human RACK1 using the QuickChange II XL site-directed mutagenesis kit (Roche). All plasmids are available upon request.
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bioRxiv - Synthetic Biology 2019Quote: ... and the candidates were PCR amplified from a U2OS (human bone osteosarcoma cell line) genome prep as template with the Kapa Hifi Hotstart Polymerase (Roche) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: A comprehensive list of coordinates of all the exonic and conserved regulatory elements from human X chromosome was used to design a customized capture library from Roche, NimbleGen (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2021Quote: Human pluripotent stem cells (hPSCs) were maintained in E8 medium and passaged every 4 days onto matrigel-coated plates (Roche). The following hPSC lines were used in the study ...
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bioRxiv - Developmental Biology 2021Quote: H9 human pluripotent stem cells were maintained in E8 media and passaged every four days onto matrigel-coated plates (Roche). ESCs ...
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bioRxiv - Cancer Biology 2022Quote: ... Between 50 and 100 ng of RNA was used as input for the KAPA RNA HyperPrep Kit with RiboErase (Human/Mouse/Rat) library preparation (Roche) on an automated liquid handling platform (Beckman Coulter) ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... kidney (human primary renal proximal tubule epithelial cells, RPTEC), and liver (human hepatocellular carcinoma, HepG2) [45–50] cells were assessed using a standard WST-1 (Roche) cell viability assay ...
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bioRxiv - Biochemistry 2023Quote: Cytotoxicity assays were performed as described29 (with minor changes. Proliferation of human cells was assessed using an MTT colorimetric assay (Cell Proliferation Kit I, Roche). HeLa (epithelial cells ...
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bioRxiv - Immunology 2023Quote: K2 cells or human neutrophils were lysed with 150-200 µl of lysis buffer (supp. Table 2) supplemented with 1X complete inhibitor (Roche) and incubated for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: Frozen brain tissues from human and animals were used to prepare 10% (w/v) homogenates in RIPA buffer containing PI and PhosStop (Roche). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... To detect for DNA containing complementary sequences on membrane-bound DNA a α-32P-dCTP-labelled probe spanning the region of 37-611 nts on human mtDNA was synthesized using High Prime DNA Labeling Kit (Roche). After pre-hybridizing the membrane with Church’s buffer (250 mM NaPi pH 7.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were extracted by incubation with RIPA buffer and protease inhibitor cocktail (Roche, 4693159001) on ice for 1 hour with occasional vortexing ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) containing cOmplete EDTA-free protein inhibitor cocktail (Roche, 1 tablet/10 ml), lysozyme (1.0 mg/ml ...
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bioRxiv - Microbiology 2019Quote: ... GRA16HA (and other HA-tagged proteins) was detected using rat anti-HA antibodies (Roche) while GRA24MYC was detected using rabbit anti-MYC tag antibody 9E10 (Santa Cruz Biotechnology) ...
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bioRxiv - Biochemistry 2019Quote: ... washed and dried before digestion of proteins with 12.5 ng/μl of trypsin (Roche) in 25 mM NH4CO2 and 10 mM CaCl2 overnight at 25°C ...
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in 1% SDS containing PBS supplemented with protein inhibitor cocktail (Roche) and 250 U/ml bensonase (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested using Endoproteinase Lys-C at 1:100 w/w (Roche, 11058533103) at 37 °C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... The protein lysates were extracted in presence of a protease cocktail tablet (Roche Diagnostics) using Cell Lysis buffer (ThermoFisher) ...
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bioRxiv - Physiology 2020Quote: ... Proteins were extracted using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche). Samples were vortexed for 5 seconds ...
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bioRxiv - Biochemistry 2020Quote: ... MR-Fc was purified from cell culture supernatant using Protein A-agarose beads (Roche). For ELISA experiments ...
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bioRxiv - Microbiology 2020Quote: ... Antibody was removed by the addition of 100 µl homogeneous Protein G-Agarose (Roche) and a further incubation for 3 h ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected with a mouse anti-GFP antibody (1:5000; Roche). Immunoblot results were quantified using Image J software (v1.8.0).
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bioRxiv - Developmental Biology 2022Quote: ... The lysate was precleared by incubation with 50 μL protein-A agarose beads (Roche) for 1 h and then incubated with anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were generated using RIPA lysis buffer supplemented with protein inhibitor (Roche, #4693159001) and protein concentration was determined using Pierce™ BCA protein assay ...
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bioRxiv - Cancer Biology 2020Quote: ... Separated proteins were then transferred to a methanol-activated polyvinylidene difluoride membrane (PVDF, Roche) using wet transfer Mini-PROTEAN II electrophoresis system (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: ... GFP-tagged proteins were detected with a primary mouse antibody IgG1K Anti-GFP (Roche) diluted to 1:1000 in 5 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were then treated with 8 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 3 hours followed by 19 μg/sample of trypsin (Roche Cat ...