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Citations for Roche :
401 - 450 of 6174 citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: Proteasome activity was measured by spectrofluorometry using the 7-amino-4-methylcoumarin (AMC)-labeled fluorogenic substrate succinate-LLVY-AMC (Roche Sigma-Aldrich) in cleared extracts of leaf 1 at different dpg (Üstün and Börnke ...
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bioRxiv - Cell Biology 2020Quote: ... harvested cells were incubated with 25 μg/ml digitonin in buffer H (20 mM Hepes-KOH, pH 7.4, 0.25 M sucrose, 1 mM DTT, complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in 10 μM BrdU:BrdC (3:1) for 16–20 h before incubation with 0.1 μg/mL colcemid (Roche) for 2-3 h ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA probes were generated via overnight incubation at 37°C using 20 U/µL T7 RNA polymerase (Roche) and digoxigenin (DIG)-labeled dNTPs (Roche ...
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bioRxiv - Neuroscience 2021Quote: DLK and Flag-tagged FKBPL/FKBP8 transfected HEK293T cells were lysated in IP buffer (0.5% NP40, 150 mM NaCl, 20 mM Tris-HCl pH 7.5) containing a protease inhibitor cocktail (Roche). GFP-DLK was immunoprecipitated with anti-GFP antibody pre-bound to Dynabeads Protein A (Thermo ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed thrice with Wash Buffer (20 mM HEPES-KOH pH 7.5, 150 mM NaCl, 0.5 mM spermidine, Roche complete Protease Inhibitor tablet EDTA free ...
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bioRxiv - Cell Biology 2022Quote: ... by scraping the cells in ice-cold PBS and lysing cell pellets in the lysis buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1.0% Triton X-100, and protease inhibitors; Roche). Supernatants and pellet fractions were separated by centrifugation at maximum speed for 20 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 350 μl of lysis buffer (20 mM Tris pH 7.5, 0.5 M o NaCl, 1 mM EDTA, 1X protease inhibitor cocktail [Complete; Roche]) and 200 μl of glass beads (425-600 μm ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed three times on the magnet in 1 mL buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1x Roche complete protease inhibitors ...
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bioRxiv - Immunology 2022Quote: ... in 800μl ice-cold homogenization buffer [(PBS containing 0,4M NaCl, 0,05% Tween-20, 10mM EDTA and protease inhibitor cocktail complete (Roche)] ...
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bioRxiv - Immunology 2019Quote: ... 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1 mM sodium orthovanadate, and complete protease inhibitor [Roche]). The following antibodies were used ...
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bioRxiv - Immunology 2019Quote: ... 1% Pen/Strep) containing 0.25 mg/mL Liberase TL and 20 U/mL DNase I (both from Roche), stirring at 37°C for 1h ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of 2× SYBR Green Real-time PCR Master Mix (Roche), 0.25 μM primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Cell Biology 2019Quote: ... cardiomyocytes were dissociated using DPBS for 20 min at 37 °C followed by 1:200 Liberase TH (Roche) diluted in DPBS for 20 min at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... and permeabilized in wash buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine and one Roche Complete protein inhibitor tablet per 50 mL ...
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bioRxiv - Genomics 2019Quote: ... Adequate ESC cells were harvested and washed twice with Wash Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1X cocktail (Roche), 1 mM PMSF (AMRESCO ...
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bioRxiv - Developmental Biology 2020Quote: ... rinsed in TBS/0.1% Tween-20 and incubated overnight in blocking buffer (TBS with 2% goat serum, 0.1% blocking reagent (Roche), 0.1% Tween-20 ...
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bioRxiv - Physiology 2021Quote: ... Whole cell extracts were prepared in HEPES whole cell lysis buffer (20 mM HEPES, pH 7.4, 1% TX-100,1x phosphoSTOP complete inhibition cocktail tablets [Roche] ...
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bioRxiv - Molecular Biology 2020Quote: ... 110 mM KOAc, 0.5% Triton X-100, 0.1% Tween 20, 10 mM MnCl2, 1x proteinase inhibitors (complete, EDTA-free (Roche)) ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.9; 10 mM KCl; 0.1% Triton X-100; 20% Glycerol; 0.5mM spermidine; 1x complete protease inhibitor [Roche # 11836170001]), collecting by centrifugation (600 g ...
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bioRxiv - Neuroscience 2021Quote: ... 5 mM MgCl2, 20 mM Tricine-KOH, 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, 1X Roche EDTA-free protease inhibitor cocktail ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed again in PBS before staining for 20 minutes in DAPI (5 µg/mL, Roche 10236276001) in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 350 μl of lysis buffer (20 mM Tris, pH 7.5, 0.5 M NaCl, 1 mM EDTA, 1x protease inhibitor cocktail [Complete; Roche]) and 200 μl of glass beads (425-600 μm ...
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bioRxiv - Cell Biology 2020Quote: ... 0.3% (v/v) Tween-20) for 5 min under agitation and next blocked in 1× blocking buffer (Roche) for 30 min at room temperature with gentle shaking ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pelleted then washed once with 250 uL TAP extraction buffer (40 mM HEPES pH 7.5, 10% Glycerol, 350 mM NaCl, 0.1% Tween-20, phosphatase inhibitor cocktail and proteinase inhibitor cocktail from Roche). Cell pellets were resuspended in 180 uL modified 2X SDS buffer then boiled at 100 °C for 4-5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... The beads were then washed twice in Digitonin Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1x Roche Complete Protease Inhibitor no EDTA ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting pure nuclear pellet was first resuspended with 70 μL low salt buffer (20 mM HEPES, pH 7.6, 1.5 mM MgCl2, 10 mM KCl, 25% glycerol, protease inhibitor (Roche)) and 2.5 μL benzonase (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... cells pellet was resuspended in 2 ml of lysis buffer (20mM sodium phosphate, 0.5 M NaCl, 20 mM imidazole; pH 7.4 with a protease inhibitor tablet (Roche) per 1 g of wet cell pellets ...
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bioRxiv - Genomics 2022Quote: ... twice 10 min with cold TET buffer (10 mM Tris/HCl pH7.5, 1 mM EDTA, 0.1% Tween-20, protease inhibitor cocktail (Roche)) and eluted with TE buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... The following day the predigested hearts were transferred to digestion medium (20 mM BDM, 1.5 mg/ml Roche Collagenase/Dispase enzyme mix in L15 medium ...
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bioRxiv - Molecular Biology 2022Quote: ... The next day beads were washed twice in cold Digitonin Buffer (20 mM HEPES pH 7.5, 150mM NaCl, 0.5mM Spermidine, 1x Roche complete protease inhibitors ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked using 10% skimmed milk in PBS containing 0.1% Tween 20 (PBST) and then incubated with rat anti-HA high affinity (1:1,000; Roche) and rabbit anti-T ...
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bioRxiv - Developmental Biology 2020Quote: ... were washed three times with Wash Buffer (20 mM HEPES-KOH pH 7.5, 150 mM NaCl, 0.5 mM spermidine, Roche complete Protease Inhibitor tablet EDTA free ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated for 50-60 min at 37οC with 20 µM EdU and 100 ng/mL colcemid (Roche). Following metaphase arrest ...
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bioRxiv - Molecular Biology 2020Quote: ... and washed three times with 1 mL digitonin buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1x Roche complete protease inhibitors ...
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bioRxiv - Neuroscience 2021Quote: ... For 20 min the DRGs were digested with 0.35 U/ml Liberase TM (Roche Diagnostics Corp., Indianapolis, IN) and then for 15 min with Liberase TL (0.25 U/ml ...
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bioRxiv - Genetics 2022Quote: ... pre-equilibrated with Buffer D (20mM HEPES pH 7.9, 150mM KCl, 20% glycerol, 0.2mM EDTA, 0.2% Triton X-100 and complete Roche inhibitor) and carried out on a rotating wheel over night at 4°C ...
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bioRxiv - Genomics 2022Quote: ... The ConA bound nuclei were then incubated with primary antibody diluted 1:100 in Antibody Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Genomics 2022Quote: ... 5 µL of 20 mM siRNAs and 3 µL of X-tremeGENE HP DNA Transfection Reagent (Roche, #6366546001) were diluted in 200 µL Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... For enteric glial cell experiments were performed by hypotonic lysis in 200 µl of buffer A (20 mM HEPES, pH 8.0, 1 mM DTT, 0.1% NP-40 and a proteinase inhibitor cocktail from Roche). The cell lysates were incubated on ice for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... oocytes were then roughly separated in bundles of 15-20 oocytes and treated with 1mg/ml Liberase (Roche) to remove the surrounding tissue.
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bioRxiv - Plant Biology 2022Quote: ... Cell pellets were thawed on ice and resuspended in lysis buffer (20 mM sodium phosphate pH 6.8, 10 mM DTT, 1 mM EDTA, and 0.25× protease inhibitor cocktail [Roche]). Cells were sonicated on ice and centrifuged for 10 min at 500 g and 4 °C to remove intact cells and cell debris ...
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bioRxiv - Developmental Biology 2022Quote: ... 500,000 trypsinized ESC were washed a total of three times with Wash Buffer (20 mM HEPES-KOH pH 7.5, 150 mM NaCl, 0.5 mM spermidine, Roche complete Protease Inhibitor tablet EDTA free ...
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bioRxiv - Cell Biology 2019Quote: ... 20 mM HEPES pH 7.4 with 2 mM 2-mercaptoethanol and EDTA-free cOmplete protease inhibitor tablets (Roche) prior to lysis by probe sonication ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Whole cell extracts were prepared in HEPES whole cell lysis buffer (20 mM HEPES, pH 7.4, 1% TX-100,1x phosphoSTOP complete inhibition cocktail tablets [Roche] ...
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bioRxiv - Developmental Biology 2019Quote: ... After a series of washes the probes were detected using FITC conjugated anti-Digoxigenin antibody (1:20, Roche) amplified with anti-sheep Alexa Fluor 488 (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... The terminal phosphate of the pGpG product is cleaved by the coupling enzyme AP (20 U/µl, Roche), and the phosphate concentration is determined from the fluorescence increase through binding of phosphate to the phosphate sensor (0.5 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were pelleted at room temperature and then washed using 1 volume of Wash Buffer twice (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine in dH2O with 1X Roche Complete Protease Inhibitor EDTA-Free tablet) ...