Labshake search
Citations for Roche :
401 - 450 of 5331 citations for 3 Phenoxybenzoic Acid Phenoxy 13C6 99% 100 Ug Ml In Acetonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... dried and solubilized in 0.2 ml Hepes (100 mM) buffer containing cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) (1 tablet/20 ml of buffer solution) ...
-
bioRxiv - Microbiology 2019Quote: ... The cells were washed twice with PBS and lysed with 6 ml of lysis buffer (8 M urea, 150 mM NaCl, 100 mM ammonium bicarbonate, pH 8; added per 10 ml of buffer: 1 tablet of Roche mini-complete protease inhibitor EDTA free and 1 tablet of Roche PhosSTOP tablet ...
-
bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 % glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
-
bioRxiv - Neuroscience 2021Quote: ... Coli tRNA at RT for 10 minutes and resuspended in EMSA 1X with 100 U/ml RNAse inhibitor and 1X protease inhibitor cocktail (Roche). The assay was then performed in presence of 250 ng of biotinylated transcript ...
-
bioRxiv - Physiology 2021Quote: ... and 400 ml of sample was placed in a 2-l wide-mouthed Erlenmeyer culture flask with 100 ml of freshly prepared blendzyme (Roche Liberase TM ...
-
bioRxiv - Microbiology 2022Quote: PPs were excised from the SI and digested (37 °C, 45 min) using 100 µg/ml liberase TH/DNase (Roche) in RPMI containing 5 % FCS ...
-
bioRxiv - Bioengineering 2020Quote: The cell-free reaction mixture for the KGK10 strain is identical to that used for the T7 SHuffle® with the addition of 100 μg/mL DsbC (GeneFrontier) and 3.33 Units/uL T7 RNAP (Roche). Previously prepared KGK10 extract fermented and processed by the Swartz lab was tested alongside the in-house KGK10 extract prepared by shake flasks.
-
bioRxiv - Neuroscience 2020Quote: ... To lyse the cells medium was removed and the well was washed with 2 ml ice cold PBS before 100 µl lysis buffer was applied (RIPA buffer supplemented with PhosSTOP (Roche) and protease inhibitors (cOmplete Mini ...
-
bioRxiv - Neuroscience 2020Quote: ... 18 µl cleared cell lysate or brain homogenates (20-100 µg based on Tau aggregate content) were incubated with 2 µl 1 mg / ml pronase (Roche) at 37° C for one hour ...
-
bioRxiv - Biochemistry 2019Quote: ... 10 mM imidazole and 10% glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
-
bioRxiv - Immunology 2019Quote: ... memory B cells were plated at 6 B cells in 55 μl per well into 96 × 384-well plates in B cell media supplemented with 100 U ml−1 IL-2 (Roche), 50 ng ml−1 IL-21 (Invitrogen) ...
-
bioRxiv - Neuroscience 2021Quote: ... medium was removed and the well was washed with 2 ml ice cold PBS before 100 μl lysis buffer were applied (RIPA buffer supplemented with PhosSTOP; Roche) and protease inhibitors (complete Mini ...
-
bioRxiv - Microbiology 2021Quote: ... At an OD600 of 5-6 the cells were harvested at 11.325 g and 4 °C for 15 min and cell pellet of 100 ml cell culture was resuspended in 25 ml buffer A (100 mM Tris-HCl, pH 8.0) with cOmplete™ Proteinase inhibitor (Roche). Cell disruption was performed using the French Press cell with a pressure of 172 mPA for five passages ...
-
bioRxiv - Plant Biology 2021Quote: ... water was exchanged for 100 µL water with 5 µM L-012 (WAKO chemicals) and 2 µg/mL horseradish peroxidase (Type II, Roche). After the leaf discs were treated with 1 µM 3-OH-C10:0 (stock in MeOH ...
-
bioRxiv - Genetics 2022Quote: ... with 5mM EDTA to remove waste and epithelial cells then were minced and dissociated in RPMI containing collagenase (100 mg/ml collagenase A; Roche), DNase I (50 μg/ml ...
-
bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 20 mM KCl, 2 mM MgCl2, 1% TX-100, 40U Ribolock ml-2 and protease inhibitor cocktail, Roche) followed by clearing at 17 000 × g for 10 min at +4C° ...
-
bioRxiv - Immunology 2023Quote: ... Washed small intestine pieces were then finely chopped and incubated in serum-free medium added with 100 μg/ml Liberase TL (Roche) and 50 μg/ml DNAse I (Roche ...
-
bioRxiv - Immunology 2023Quote: ... Stored synovial tissue samples were then thawed and disaggregated into single-cell suspensions by mincing and digesting with 100 µg/mL LiberaseTL (Roche) and 100 µg/mL DNaseI (Roche ...
-
bioRxiv - Immunology 2022Quote: ... Excised tumors were cut into small pieces and digested at 37°C for 30 min with 100 μg/ml DNase I (Roche) and 200 U/ml Collagenase (Worthington) ...
-
bioRxiv - Cell Biology 2023Quote: ... The microsome fraction was solubilized in 1% Triton X-100 in PBS (10 mg microsome per ml) with protease inhibitor cocktail (complete mini, Roche) by rocking at 4°C for 60 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... each supplemented with protease and phosphatase inhibitors (2X cOmplete, EDTA-free, Protease Inhibitor Cocktail [Roche]; 1X PhosSTOP Phosphatase Inhibitor [Roche]; 100 µg/mL Pefabloc SC [Roche]), while incubating on ice for 10 minutes ...
-
bioRxiv - Neuroscience 2024Quote: Brains were dounce homogenized in RPMI (1X) + 10% Cosmic Calf Serum (Hyclone) and digested with 100 mg/mL collagenase/dispase (Roche) and 1 mg/mL DNase I (Roche ...
-
bioRxiv - Microbiology 2024Quote: HeLa cells treated with or without CsA were infected with HIV-1 (10 ng p24) that had been treated with 100 U/ml of DNase I (Roche) for 1 h at 37° C ...
-
bioRxiv - Microbiology 2020Quote: ... total nucleic acid was extracted from 400 µl of cerebrospinal fluid using the MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche Diagnostics, Indianapolis, IN, USA) on the MagNA Pure compact automated extractor ...
-
bioRxiv - Developmental Biology 2019Quote: ... and detected with the DIG Nucleic Acid Detection Kit (Roche). Wing imaginal discs were mounted in glycerol and imaged with a Nikon E200 bright-field microscope.
-
bioRxiv - Molecular Biology 2019Quote: ... Nucleic acids were removed with 250 U of Benzonase (Roche) for 1 hr at 4° C ...
-
bioRxiv - Plant Biology 2020Quote: ... 10 mM ascorbic acid) containing a protease inhibitor cocktail (Roche). All subsequent steps were conducted on ice ...
-
bioRxiv - Plant Biology 2022Quote: ... and ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail cOmplete (Roche). For blocking and antibody dilutions ...
-
bioRxiv - Microbiology 2019Quote: ... albumin (5 g/L; fraction V, fatty acid-free, Roche) and tyloxapol (0.04%) ...
-
bioRxiv - Neuroscience 2021Quote: ... Okadaic acid (200nM) and a protease inhibitor cocktail (Roche Complete) with a Dounce homogenizer and solubilized for 1 hour rotating at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... albumin (5 g/L; fraction V, fatty acid-free, Roche) and tyloxapol (0.04%) ...
-
bioRxiv - Immunology 2021Quote: ... 0.25% sodium deoxycholic acid and complete protease inhibitor cocktail (Roche), pH 7.5 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.3% 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS)) supplemented with 1X protease inhibitor cocktail (Complete; Roche), 0.5 mM phenylmethylsulfonyl fluoride (PMSF) ...
-
bioRxiv - Cell Biology 2022Quote: ... The remaining tissue was digested on a shaker at 400 rpm for 40 min at 37°C in 100 μg/ml Liberase TM (Roche, 5401127001) and 100 μg/ml DNAse I (Roche ...
-
bioRxiv - Cancer Biology 2020Quote: ... 5 mM EDTA, 0.5 mM DTT, and 0.5% NP40 substitute supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail) and sonicated for 5 minutes using BioRuptor Pico water bath sonicator (30 s ON/OFF ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 volume of nuclei suspension was mixed with 1 volume of DNase I cocktail (NWB supplemented with CaCl2 2 mM, BSA 100 μg/ml, and twice the indicated concentration of DNase I (Roche 10104159001) and incubated for 8 minutes at RT ...
-
bioRxiv - Cancer Biology 2022Quote: Cancer transplantation was performed as described in the section “spatial transcriptomics.” The obtained livers were minced and dissociated in RPMI1640 containing DNase I (100 μg/ml: Roche, Switzerland) and collagenase IV (1 mg/ml ...
-
bioRxiv - Genomics 2023Quote: hTERT RPE-1 cells at 70% confluence were harvested by trypsinization after 2-hour treatment with colcemid (100 ng/ml, Roche 10295892001) to arrest cells in mitosis ...
-
bioRxiv - Cancer Biology 2024Quote: KPC-4662 tumors from sedentary and exercised mice were harvested and immediately digested in HBSS with DNase I (100 µg/mL; Roche, 04716728001) and collagenase type II (10mg/ml ...
-
bioRxiv - Neuroscience 2021Quote: ... and ∼3 mg DNase I (Roche). MhOR5 was extracted using 0.5% (w/v ...
-
bioRxiv - Developmental Biology 2022Quote: ... 3 µL 10% NP40 (Roche 11332473001), 3 µL 10% Tween-20 (Roche 11332465001) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and dithiothreitol (Roche, 3483-12-3). Lysates were rocked at 4C for 20 min and centrifuged 10 min at 15,000g ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell pellet samples were collected and re-suspended in lysis buffer (100 mM Tris-HCl, pH 7.4, 100 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, Roche complete protease inhibitor set ...
-
bioRxiv - Genomics 2021Quote: ... rp49 5’-TAATACGACTCACTATAGGGCAGTAAACGCGGTTCTGCATG-3’ and 5’-CAGCATACAGGCCCAAGATC-3’) were transcribed using the Biotin RNA Labeling Mix (Roche) and T7 polymerase (Promega).
-
bioRxiv - Developmental Biology 2020Quote: ... The embryos were blocked in Blocking Buffer + Maleic Acid (Roche, 11585762001) for at least 3 hours before the anti-DIG antibody was added in a concentration of 1:5000 and incubated overnight at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.5% deoxycholic acid) containing inhibitors of phosphatases (1:10 PhosphoStop; Roche) and proteases (1:100 Protease Inhibitor Cocktail ...
-
bioRxiv - Biochemistry 2020Quote: ... followed by optional sialic acid removal using 0.02 U sialidase (Roche), prior to in-gel trypsin treatment49 ...
-
bioRxiv - Plant Biology 2020Quote: ... Digoxigenin was detected using DIG Nucleic Acid Detection Kit (Roche, USA).
-
bioRxiv - Systems Biology 2021Quote: ... 2% fatty acid-free bovine serum albumin (BSA) fraction V (Roche), 50 μmol/L 2-mercaptoethanol (Sigma-Aldrich) ...