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Citations for Roche :
4401 - 4450 of 10000+ citations for Rat Isocitrate Dehydrogenase 1 Soluble IDH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: Cell pellet were thawed in 300 μl hypotonic lysis buffer (HEPES 20 mM, MgCl2 1 mM, Glycerol 10%, Triton 0.5 %, DTT 1 mM, 1x Complete protease inhibitor [Roche], Benzo nuclease) and incubated at room temperature for 15 mins nucleic acid digestion ...
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bioRxiv - Cell Biology 2019Quote: ... 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F], and protease inhibitor cocktail [Roche, 000000011836170001) and incubated on ice for 25 min ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were homogenized in 2 mL co-IP lysis buffer (20 mM Tris-HCl, pH = 7.6; 150 mM NaCl; 1 mM EDTA; 10% glycerol; 1% Nonidet P40 Substitute; protease inhibitor cocktail [Roche]; PhosSTOP [Roche]), using a Dounce homogenizer ...
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bioRxiv - Neuroscience 2021Quote: ... mice and lysed on ice for 30 min in protein lysis buffer (1 mM Na3VO4, 1 mM NaF, complete protease inhibitor cocktail (Roche, USA), 1% Triton X-100 in PBS) ...
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bioRxiv - Plant Biology 2020Quote: ... 3 volumes of lysis buffer (50 mM Tris-Cl pH 7.4, 150 mM KCL, 1 % Triton X100, Protease inhibitor 1 X [Roche], NEM 20uM) was added ...
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bioRxiv - Cell Biology 2021Quote: ... and resuspended in 100 μl of protein breakage buffer (10 mM Tris-HCl pH 7.5, 1 mM EDTA pH 7.5, 2.75 mM DTT, 1 x Roche EDTA-free protease inhibitors) and one volume of glass beads (0.5mM zirconia/silica glass beads ...
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bioRxiv - Microbiology 2021Quote: ... 1mM EDTA, pH 8.0; 1 mM EGTA, pH 8.0; 0.65% NP-40; 1 mM DTT, 1x Roche Complete protease inhibitors cocktail). Nuclei were pelleted at 3,000 x g for 20min at 4°C and cytoplasmic fraction was removed ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 mM NaCl, 2 mM MgAc2, 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]). After 30 min ...
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bioRxiv - Immunology 2022Quote: ... counted and lysed in m-RIPA buffer (1% NP40, 1% sodium deoxycholate, 150mM NaCl, 50mM Tris pH 7.5, Complete protease inhibitors; Roche, Basel, Switzerland). CANX and T6BP were immunoprecipitated using anti-CANX antibody PA5-34754 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were re-suspended in 1 mL lysis buffer (5 mM EDTA, 1% NP-40 in PBS) supplemented with 2X cOmplete™ inhibitor (Roche) and were sonicated until the lysate became turbid ...
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bioRxiv - Neuroscience 2020Quote: The following conjugated antibodies were used: sheep polyclonal anti-digoxigenin alkaline phosphatase (1:2000 - 1:5000; Roche Life Science, Cat#11093274910), mouse monoclonal anti-βACTIN (clone AC-15 ...
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bioRxiv - Microbiology 2021Quote: ... 1mM EDTA, 10 mM Sodium butyrate, 0.1% deoxycholate, 0.1% SDS, 1% Triton X-100, 1mM PMSF and 1 × Roche protease inhibitor Cocktail). The obtained chromatin was sonicated into DNA fragments between 200-500 bp using Diagenode Bioruptor (high setting ...
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bioRxiv - Physiology 2020Quote: ... in lysis buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was again discarded and 0.6 ml Storage buffer (10% glycerol, 1 mM EDTA, 10 mM TrisHCl pH 7.5, 25 mM NaCl, 1 mM PMSF, 1x Roche protease inhibitor) was added on top of the pellet ...
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bioRxiv - Cancer Biology 2020Quote: ... 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1 mM PMSF, Roche protease inhibitors 11836170001) for 10 min at 4°C with rotation ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 cells were lysed in the lysis buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1 % Triton, 1 x EDTA-free Roche protease inhibitors) and incubated on ice for 30 mins prior to a double sonication at 12 % for 3 seconds each (1 min on ice between sonications) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 seconds each with intermittent cooling on ice ...
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bioRxiv - Immunology 2020Quote: ... 0.1% NP-40 (Nonidet P40, Applichem) and 1 pill of protease inhibitor EDTA-free (cOmplete Tablets, Mini EDTA free, Roche diagnostics) per 30 mL lysate ...
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bioRxiv - Biophysics 2019Quote: ... cells were harvested by centrifugation and resuspended in lysis buffer (20 mM Tris pH 7.5, 1 mM EDTA, 200 mM NaCl, 1 mM βMe, Roche protease inhibitor) and frozen ...
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bioRxiv - Microbiology 2020Quote: ... from persons with established infection (both ART naïve and receiving ART) with HIV-1 VL data (COBAS AmpliPrep/COBAS TaqMan® HIV-1 Test, v2.0, Roche Molecular Diagnostics) were included to evaluate the impact of VL on the performance of the ARCHITECT.STOP was a prospective study evaluating the recommended diagnostic algorithm for detection of acute HIV infection and linkage to enhanced partner services in New York City ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed twice in 1□ml Digitonin Buffer (20 mM HEPES-KOH pH 7.5; 150□mM NaCl; 0.5□mM Spermidine; 1×Roche cOmpleteTM; 0.05% digitonin), and then resuspended with CUTANA pAG-MNase in 50□μl Digitonin Buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... 25 million cells were resuspended in 1 ml lysis buffer (1% SDS, 50 mM Tris-HCl pH 8, 20 mM EDTA, 1x cOmplete (Roche, 4693132001) protease inhibitor) ...
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bioRxiv - Immunology 2022Quote: Skin biopsies were collected and minced with sterile scissors before digestion in 1 mL of DMEM with 1 mg/mL collagenase D (Roche Diagnostics) for 3 hours at 37℃ ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM EDTA, 1 mM Na3VO4, 20 mM NaF, 0.5% Triton X-100, 1 × protease inhibitors complete EDTA-free from Roche, Mannheim, Germany). Total protein concentration of prepared cell extracts was measured by Bio-Rad protein assay (Bio-Rad Laboratories GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM NaCl, 2 mM MgCl2, 10 mM NaF, 1 mM PMSF, 1% Triton X-100 and Complete Protease inhibitor mixture, Roche Diagnostics) for 45 min on ice and centrifuged at 20000g for 10 min at 4°C ...
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bioRxiv - Genomics 2024Quote: The nuclei were resuspended in 250 µL of 5’ phosphorylation master mix (1X T4 PNK buffer, 500 U/mL of T4PNK, 1 mM ATP, 1 U/µL of RNAse inhibitor (Roche, 3335399001)) and incubated at 37°C while rotating at 800 rpm for 1 hour to phosphorylate the 5’ ends of RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed for 10 min on ice in 3 ml ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris pH 8.1, 1 mM PMSF, 1,5x Roche protease inhibitor mix). The DNA was sheared by sonication (4x 7 cycles ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM EDTA, 1 mM Na3VO4, 20 mM NaF, 0.5% Triton X-100, 1 × protease inhibitors complete EDTA-free from Roche, Mannheim, Germany). Total protein concentration of prepared cell extracts was measured by Bio-Rad protein assay (Bio-Rad laboratories GmbH ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4°C against either HA (1:5000, Roche 12013819001), FLAG (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% bovine serum albumin (BSA)/TBS-T ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.9, 50% glycerol, 75 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 1 mM PMSF and Roche protease inhibitor cocktail), then 100 μl prechilled nuclei lysis buffer (10 mM HEPES ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche] in Maleic acid) for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... cut into small pieces with a scalpel and transferred into 1 ml collagenase-dispase solution (1 mg/ml in PBS, Roche #10269638001) for 10 min at 28°C ...
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bioRxiv - Plant Biology 2023Quote: ... 50 mM Tris/HCl pH7.5, 1 mM EDTA, 300 mM NaCl, 1 mM DTT, 0.1% [v/v] Nonidet P-40/Igepal, Roche cOmpleteTM protease inhibitor) was added to grinded-frozen samples ...
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bioRxiv - Molecular Biology 2023Quote: ... extract was prepared using Co-IP buffer (50 mM Tris/Cl (pH=7.5), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 10% glycerol, 1xPIC (cOmplete™, Protease Inhibitor Cocktail (Roche; 04693116001), 0.5 mM PMSF) ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1% SDS) with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 X EDTA-free mammalian protease inhibitor cocktail (Roche Diagnostics, 11836170001). Cell pellets from control and subject fibroblasts and corresponding overexpression cells were solubilized in 1% n-dodecyl-β-D-maltoside (DDM ...
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bioRxiv - Cell Biology 2022Quote: ... 200 mM NaCl; 2 mM MgAc; 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]) per 1 g of cell pellet and incubated for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated overnight at 4°C in 1% goat serum in MABT with 1∶5000 alkaline phosphatase-coupled anti-Digoxigenin antibody (Roche Diagnostics). On the third day ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in ice-cold PBS and lysed (1% SDS, 10 mM Tris-HCl, 1 mM NaVO3, cOmpleteTM Mini Protease Inhibitor (Roche, #11836153001), pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were prepared by incubation in Onyx lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche Molecular Biochemicals]) at 4 °C for 15 min ...
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bioRxiv - Genetics 2023Quote: ... 25-30 million cells were resuspended in 1 mL lysis buffer (1% SDS, 50 mM Tris-HCl pH 8, 20 mM EDTA, 1x cOmplete (Roche, 4693132001) protease inhibitor) ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.4 with 0.1% Triton X-100) and then incubated with 350 µg protein in 1:1 8M urea with Complete protease inhibitor (Roche 11245200) sample lysis:Buffer A/0.5% Triton X-100 (150 mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 1.5h in PBSTw-1%BSA before being incubated with anti-DIG-POD antibody (Roche, 1:2000) and other primary antibodies o/n at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Cultures were grown until an A600 of 0.6-0.8 was reached and protein over-expression was induced by incubating with 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG, Roche) for 16 hours at 18°C.
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bioRxiv - Biophysics 2024Quote: ... 1 mM phenylmethyl sulphonyl fluoride (PMSF)) plus EDTA-free protease inhibitor tablets (1 tablet per 50 mL, Roche, cat. no. 05056489001), 0.5% Triton-X100 ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... Cells were grown to ∼80% confluency, washed in PBS and lysed (1% SDS, 10 mM Tris-HCl, 1 mM NaVO3, and protease inhibitors (Roche, #11836153001), pH 7.5 ...
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bioRxiv - Genetics 2024Quote: ... cells were resuspended in 3 ml lysis buffer (PBS containing 1% Triton X-100, 1 mM phenylmethylsulfonyl fluoride and cOmplete proteinase inhibitor [Roche Diagnostics]), lysed by ultrasonic treatment and incubated with 1.2 ml NeutrAvidin Agarose for 0.5 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... were prepared using the IP buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% TritonX-100, 0.5% Na-DOC, 1 mM EDTA, 2 mM PMSF and 1x Roche protease inhibitor cocktail). After sonification for 5 min and clarification with centrifuge at 16,000 g at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... The whole cell lysates were extracted from trans transfected 293T cells or infected LNCaP cells or PC-3 cells by IP lysis buffer (50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, Roche protease inhibitor cocktail). An aliquot of the cell lysate was kept as input for western blot analysis ...