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Citations for Roche :
4401 - 4450 of 6468 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The alkaline phosphatase conjugated anti-mouse secondary antibodies were used for hybridization and for detecting signals BCIP/NBT substrates (Roche Biochemical, Mannheim) was used.
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bioRxiv - Cell Biology 2021Quote: ... To determine the dimerization of sFeoFL::GFP and sFeoN:GFP a Western Blot was generated from a 4-12% gradient SDS and a Native polyacrylamide gel side-by-side using a mouse anti-GFP antibody (Roche Nr. 11814460001) at 1:500 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... Revelation of the dig-labeled ISH probe was then performed using an anti-Digoxigenin antibody coupled to POD (11207733910; Roche, 1/400) and revealed using a FITC-tyramide (1/400) ...
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bioRxiv - Developmental Biology 2022Quote: Single color staining of embryos hybridized with DIG-labelled riboprobes was performed using anti-DIG antibody conjugated to alkaline phosphatase (Roche, 1:6000) and BM purple (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight with 1:1,000 diluted alkaline phosphatase-conjugated anti-DIG sheep antibody (11-093-274-910; Roche Diagnostics, Basel, Switzerland) and then reacted with 0.375 mg/mL nitroblue tetrazolium and 0.188 mg/mL 5-bromo-4-chloro-3-indolylphosphate (NBT/BCIP ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked with 10% lamb serum in TBST for 1 hour at RT and incubated with alkaline phosphatase conjugated to DIG antibody (Roche, Basel, Switzerland) in TBST ...
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bioRxiv - Genetics 2021Quote: ... Hybridizations of the probe with the RNA were detected with an alkaline phosphatase-conjugated antibody (Anti-Digoxigenin-AP, Fab Fragments, 1:5000, Roche, Cat# 11093274910). Finally ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were blocked with 10% HI-serum for 2 h and incubated overnight at 4°C with alkaline phosphatase-labeled sheep anti-dig antibody (11093274910, Roche; 1:2500). After washing ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated with specific antibodies overnight and then with 60 μl of slurry of Protein A- or Protein G-agarose beads (Roche, Mannheim, Germany) for 3 h ...
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bioRxiv - Microbiology 2019Quote: HA-tagged constructs for western blotting were detected using a 1:5000 dilution of anti-HA-peroxidase antibody (Roche clone 3F10, #12013819001). HA-tagged constructs for indirect immunofluorescence were detected using anti-HA (Zymed ...
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bioRxiv - Molecular Biology 2020Quote: ... The Dynabeads™ were washed once with 2 ml of PBS and coated with 15 μg of mouse monoclonal anti-GFP antibody (Roche Applied Science). The beads were finally washed three times with 2 ml of lysis buffer and added to the whole cell extract for a 2 h incubation with gentle rotation ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated with specific antibodies overnight and then with 60 μL of a slurry of Protein A- or Protein G-agarose beads (Roche, Mannheim, Germany) for 3 h ...
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bioRxiv - Neuroscience 2019Quote: ... The PVDF membranes were treated and immersed into the rabbit anti-mouse PSD-95 monoclonal antibody (1:1000, Roche Group, Nutley, USA) to incubate at 4 °C overnight ...
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bioRxiv - Genetics 2020Quote: ... Proteins were separated by SDS-PAGE on 4-12% Bis-TRIS SDS-polyacrylamide gels (Novex, NP0322BOX) and analysis was done against primary antibodies α-HA (1:1000, Roche, 12CA5) or α-Tub2 (1:4500 ...
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bioRxiv - Genetics 2020Quote: ... The eluted protein was resolved on a 4-12% Bis-Tris gel (Novex, NP0322BOX) and ubiquitinated Cse4 was detected by Western blot using anti-HA antibody (Roche Inc., 12CA5). Levels of ubiquitinated Cse4 relative to the non-modified Cse4 in the input were quantified using software provided by the Syngene imaging system ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated overnight at 4 °C in blocking solution supplemented with the alkaline phosphatase coupled anti-digoxigenin antibody (Roche, REF 11093274910) 1:3000 ...
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bioRxiv - Genomics 2022Quote: ... nuclei were incubated with streptavidin conjugated secondary antibody diluted 1:100 in Wash Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) for 45 minutes at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections containing the medial prefrontal cortex (mPFC) or hippocampus were incubated with (1) an antibody cocktail of rat anti-HA (1:200, Cat. No. 10145700, Roche Diagnostics, USA) with rabbit anti-PV (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were stained with secondary antibodies (1:300) for 2 hours at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). The cells were mounted using the ProLong Diamond antifade mountant (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were incubated overnight at 4°C in Odyssey blocking buffer containing 0.1% Tween-20 at 4°C and 1/1000 dilution of anti-c-myc mouse monoclonal antibody (Catalogue number 11 667 149 007, Roche, Bâle, Switzerland) or anti-β-tubulin mouse antibody (Catalogue number T9026 ...
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections were incubated with anti-HA antibody (1:400 dilution) followed by incubation with anti-rabbit-HQ (Roche; Cat#. 760-4815) and anti-HQ HRP (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001; clone 3F10, monoclonal from Roche, 1:5000 in 5% milk) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... then treated with blocking reagent (Genostaff) for 15 min and with alkaline phosphatase-conjugated anti-DIG antibody (1:2,000; Roche Diagnostics, Basel, Switzerland) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Co-purification of FlgP or PflD-sfGFP was investigated by western blot (WB) analysis using FlgP specific antisera (21) or an anti-GFP antibody (Roche #11814460001, RRID:AB_390913), respectively ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with primary antibodies diluted 1:100 (rabbit pAb α-γH2A.X from SigmaAldrich, H5912 and mouse mAb α-GFP from Roche, 11814460001) in 1% BSA (in PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Probes were detected by incubation overnight at 4°C in a 1:5000 dilution of Anti-DIG-AP antibody (Roche, Catalog# 11093274910) in Blocking Buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The probe was detected by incubating samples overnight at 4°C with a 1:1500 dilution of Anti-DIG-POD antibody (Roche, Catalog# 11207733910) in Blocking Buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MBST + 10% HISS + 2% BMB for 2 h at room temperature and incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910) diluted in blocking buffer until signal development ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... to prepare for an hour long blocking in 10% lamb serum/TBST and an overnight incubation at 4°C in a 1% lamb serum containing Anti-Dig-AP antibody (Roche, 1:5000). The next morning ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was then amplified using an SYBR green RT-PCR kit (06924204001, Roche Products, Mumbai, India) for a 139 bp region in the Tat exon-1 using the primers- N1783 (5’- GGAATCATCCAGGAAGTCAGCCCGAAAC-3’ ...
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bioRxiv - Biophysics 2021Quote: Cholesterol concentrations were measured calorimetrically using a commercial kit (R-Biopharm and Roche Diagnostics, Cat. No. 10139050035) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labeled RNA probes were synthesized by in-vitro transcription using the DIG RNA labeling kit (Roche). In situ analysis was performed as described previously (Cecchetti et al. ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems, Wilmington, MA) according to the manufacturer’s protocol [39] ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... Quantitative reverse transcription PCR (qRT-PCR) was performed using an SYBR FAST universal qPCR kit (KAPA Biosystems). Predesigned KiCqStart primers for DDX58 ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR (Q-PCR) reactions were performed using LightCycler FastStart DNA MasterPlus SYBR Green I kit (Roche). For each primer set ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using the KAPA SYBR FAST Universal qPCR Kit (KAPA BIOSYSTEMS) in a QuantStudio3 system (Life Technology) ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng of fragmented DNA was used for library preparation using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2021Quote: ... The library was quantified using the KAPA library quantification kit for Illumina sequencing platforms (KAPA Biosystems, KK4824) and 50bp single end reads were sequenced on a HiSeq2500 following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2019) via 15 rounds of high-fidelity PCR amplification (KAPA HotStart PCR Kit, KAPA Biosystems, Wilmington, Massachusetts). Each pooled ...
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bioRxiv - Microbiology 2021Quote: ... ATP concentrations were determined using the ATP Bioluminescence Assay Kit CLS II (Roche 11699695001, from Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... by using In Situ Cell Death Detection Kit (TMR red cat no. 1684795, Roche, Indianapolis, IN, USA) according to manufacturer’s recommendation ...
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Distal regulation, silencers and a shared combinatorial syntax are hallmarks of animal embryogenesisbioRxiv - Developmental Biology 2021Quote: ... The pool is assessed by qPCR using the KAPA Library Quantification Kit - Illumina/Universal (KAPA Biosystems, KK4824) combined with the Life Technologies Via 7 real-time PCR instrument ...
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bioRxiv - Genomics 2019Quote: ... cDNA was generated by the Transcriptor First Strand cDNA Synthesis Kit (04379012001, Roche Life Science, Penzberg, Germany) in accordance to the manufacture’s manual ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems) and average size fragment was determined using a LabChip GX (PerkinElmer ...
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bioRxiv - Developmental Biology 2022Quote: ... Antisense and sense probes were synthesized by in vitro transcription using a DIG RNA Labeling Kit (Roche). Following labeling ...
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bioRxiv - Physiology 2022Quote: ... using the LightCycler FastStart DNA Master plus SYBR Green I kit (catalog n° 03515869001, Roche, Basel, Switzerland). 40ng of reverse transcribed RNA was used as template for each reaction ...
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bioRxiv - Neuroscience 2021Quote: ... Library QC included size distribution check (BioAnalyser) and concentration determination with KAPA Library Quantification Kit (KK4857, Roche). Libraries were equimolar pooled and loaded on Illumina NovaSeq 6000 SP flowcell at 300 pM loading concentration with 1 % of PhiX by-mix ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was reverse transcribed with a Transcriptor First Strand cDNA Synthesis Kit (Roche Applied Sciences, Indianapolis, IN). Quantitative RT-PCR was performed using Applied Biosystems pre-designed TaqMan Gene Expression Assays for rats and TaqMan® Fast Advanced Master Mix ...