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Citations for Roche :
4251 - 4300 of 6180 citations for Glucagon like peptide 1 receptor GLP1R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 400 µL cold cytoplasmic lysis buffer (10 mM HEPES pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.4% [vol/vol] Nonidet P40, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA free (MilliporeSigma ...
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bioRxiv - Immunology 2022Quote: ... a portion of spleens was digested on a solution containing 0.2 mg ml−1 collagenase P (Roche), 0.1 mg ml−1 DNAase I in RPMI-1640 medium and enriched by depleting CD45+ cell and TER119+ cells using MACS microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: Cell pellet or tissue were lysed in RIPA lysis buffer containing 1× proteinase inhibitor cocktail (Roche, 5056489001), 2 mM PMSF and 1 mM DTT ...
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bioRxiv - Genomics 2022Quote: ... 1 μg of RNA was used to synthesize cDNA with First Strand cDNA Synthesis kit (Roche, IN). The reaction master mix contained EvaGreen polymerase (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2023Quote: ... Both kidneys were incubated in 10 ml enzymatic solution (1 mg/ml collagenase A (Roche Diagnostics, Germany), 2.5 mg/ml trypsin (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1% Triton X-100) with added 72 mM ß-mercaptoethanol and 1× complete protease inhibitor cocktail (Roche)45 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... For NBT/BCIP staining sections were incubated overnight with sheep anti-DIG-AP (1:1000, Roche 11093274910). After washing staining was performed using NBT/BCIP in NTMT until satisfactory intensity ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... fixed cells were treated with 333 µL PBS supplemented with 1 µL RNAse (10 mg/mL) (Roche) at 37°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: • PCR #1: 10 μl reactions were prepared for each sample with KAPA HiFi HotStart ReadyMix (Roche, KK2602) containing 1 μl cDNA template and 1.5 μl of 10 μM PCR1 primer mix (U6 outer ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue fragments were then digested in DMEM medium containing 1 mg/mL Collagenase D (Roche, Switzerland), 1 mg/mL Collagenase/Dispase (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation and cell death were assessed using the WST-1 and cytotoxicity Detection KitPLUS (LDH) (Roche) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Cells (1×105) were then trypsinized and plated on collagen pre-treated cover glasses (Roche Applied Sciences) followed by treatment with GO or Bleo ...
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bioRxiv - Neuroscience 2023Quote: ... the resulting pellet was resuspended into 1500 µl nuclei suspension buffer (NSB, 0.5 % BSA, 1:100 Roche protease inhibitor ...
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bioRxiv - Immunology 2023Quote: ... Washed bladders were incubated for 1 h at 37 °C in 0.34 U/mL of Liberase (Roche) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was probed using the primary antibodies mAb mouse α-GFP (1:1’000, Roche Diagnostics #11814460001), mAb mouse α-PfGAPDH (1:20’000 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The samples were then incubated overnight at 4°C with anti-fluorescein-POD (1:2000, Roche, #11426346910).
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bioRxiv - Evolutionary Biology 2023Quote: ... The reverse transcription was performed on 1 µg of total RNA using the Transcriptor Reverse Transcriptase (Roche) with random hexanucleotides primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Bead-bound cells were resuspended in 50 mL buffer (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM Spermidine, 1× Roche complete protease inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... mechanically disrupted by a tissue homogenizer (Ika) in 1 ml of PBS with protease inhibitor cocktail (Roche) and spun at 10,000 x g for 10 min to pellet debris ...
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bioRxiv - Genomics 2023Quote: ... 1 M sodium phosphate (pH 7.2) and 0.5 ml of sonicated fish sperm solution (Roche Diagnostics, Switzerland). Probes were labeled with 32P by nick translation (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... then resuspended in lysis buffer supplemented with complete protease inhibitor cocktail tablets (Roche, 1 tablet per 50ml) at 0.3 x pellet volume ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... 5 mM β-Mercaptoethanol) supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with 1 ml lysis buffer (0.5 % Nonident-P40, 2 % protease inhibitor cocktail (Roche, Switzerland) and 1 % phosphatase inhibitor cocktails I and II (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... in deionized phosphate-buffered saline (PBS)] supplemented with 1:7 proteases inhibitors cocktail (Roche Diagnostics GmBH, Germany) for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were subjected to Western blot analysis with mouse monoclonal anti-GFP antibody (1:2,000 dilution, Roche), rabbit polyclonal anti-Sec2 antibody (1:2000 dilution ...
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bioRxiv - Genetics 2023Quote: ... 1 Complete EDTA-free Protease Inhibitor cocktail tablet per 12 mL lysis buffer (Roche Applied Science, #1873580)] and drop frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: Antigen retrieval was conducted using a Tris based buffer-Cell Conditioning 1 (CC1)-Catalog # 950-124 (Roche). The SARS-CoV-2 spike primary antibody was of rabbit origin ...
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bioRxiv - Immunology 2023Quote: ... were lysed in 100 µL or 300 mL RIPA buffer (50 mM Tris pH 7.4, 150 mM NaCl, 1% IGEPAL CA-630, 0.25% Na-deoxycholate, 2 mM EDTA, 0.1% SDS, Roche cOmplete™ Mini protease Inhibitor Cocktail) ...
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bioRxiv - Genomics 2023Quote: ... (d) Remaining 9/10 of sample is immunoprecipitated with 1:1000 anti-HA antibody (0.1mg/mL Roche Rat Anti-HA High Affinity [11867423001] ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) containing protease and phosphatase inhibitors (cOmplete™ and PhosTOP™, Roche/Merck, Dorset, UK), 1 mM sodium orthovanadate and 1 mM phenylmethylsulfonyl fluoride and incubated for 30 min on ice.
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bioRxiv - Cell Biology 2023Quote: ... or Nunc Glass Base Dishes (Thermo Fischer Scientific) coated with sterile 10 µg ml−1 fibronectin (Roche). Supplier and identifier for all reagents are listed in Table 4 ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated for 3 hours with Anti-Digoxigenin-AP Fab fragments (dilution 1:3000) (Roche, 11093274910) at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... Protein blots were probed with anti-HA antibodies conjugated to horseradish peroxidase (Roche, Switzerland, 12013819001, 1:4000). Immunoblots were visualised with Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 mM tris(2-carboxyethyl)phosphine (TCEP) and protease inhibitor (cOmplete EDTA-free Protease Inhibitor Cocktail, Roche)) ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF, 1X Roche protease inhibitor ...
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bioRxiv - Neuroscience 2023Quote: ... in lysis buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, complete protease inhibitors, Roche) and 4 U per ml Turbo-DNase (Thermofisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Bead-bound cells were resuspended in 50 ml buffer (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM spermidine, 1× Roche complete protease inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... washed with PBS and permeabilised at 1 × 107 cells/ml in MELB with cOmplete protease inhibitors (Roche) and 0.025% digitonin (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using a Transcriptor First Strand cDNA Synthesis Kit (Roche) with random hexamer primers ...
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bioRxiv - Biophysics 2023Quote: ... 50 mM KCH3COO, 2 mM MgSO4, 1 mM EGTA, 5% glycerol, 0.2mM Mg-ATP, 0.1% Octylglucoside, 0.5mM DTT, Roche cOmplete™ Protease Inhibitor Cocktail EDTA free) ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were prepared in lysis buffer containing 1% Nonidet P-40 and protease inhibitor cocktail (Roche) (Cao ...
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bioRxiv - Cell Biology 2023Quote: ... and digested for 45 to 60 minutes in a solution of collagenase A (1 mg/mL) (Roche) and DNAse I ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed and incubated with an anti-DIG antibody coupled to alkaline phosphatase (Roche, 1:3,000). Staining used BM-Purple (Roche).
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bioRxiv - Microbiology 2023Quote: ... 20mM HEPES pH 7.6) supplemented with 1% SDS and protease inhibitors (EDTA-free protease inhibitor cocktail; Roche) and transferred to 15 mL polystyrene Falcon tubes compatible with sonication ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was probed using the primary antibodies mAb rat α-HA (1:2,000, Roche Diagnostics #11867423001) or mAb mouse α-PfGAPDH (1:20,000 ...
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bioRxiv - Genetics 2024Quote: ... The dried pellets were resuspended in 200 µl of lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM EDTA, 2.75 mM DTT, 1.1 mM PMSF, Roche protease inhibitor at 2x working concentration (Roche 04693132001)) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 30 min at 37 °C with mouse anti-BrdU (11296736, Roche. 1/10) then washed before incubation at room temperature for 3 h with the primary antibody anti-myosin heavy chain (MF20 ...
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bioRxiv - Microbiology 2024Quote: ... 1% triton 100X) supplemented with 0.1 mg/mL lysozyme and complete EDTA-free Protease Inhibitor Cocktail (Roche) for 1 hour on ice ...
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bioRxiv - Plant Biology 2024Quote: ... MAZ-GFP and MAZK141N-GFP accumulation blots were probed with mouse anti-GFP (1:5,000, Roche 1814460001) and goat anti-mouse-HRP (1:10,000 ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.5) supplemented with 1 mM PMSF and cOmplete protease inhibitor cocktail (EDTA-free) (Roche, cat. # 11836170001). One gram of glass beads with a diameter of 0.5 mm (YASUI KIKAI ...