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Citations for Roche :
4251 - 4300 of 7863 citations for 6 Chloro 1 phenylsulfonyl 1H pyrrolo 2 3 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and 1 µg/ml cOmpleteTM protease inhibitor mixture (Roche). After incubation on ice for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... 1× cOmplete protease inhibitor cocktail (Roche, Indianapolis, IN, USA), and 0.25% sodium deoxycholate) ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mM PMSF) supplemented with Protease inhibitor cocktail (Roche) and 100 U/ml Ribolock (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% Triton X-100 supplemented with protease inhibitors (Roche). Media was removed from each well and replaced with STEN buffer ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg of nonacetylated bovine serum albumin (Roche). All oligonucleotides were synthesized and provided by Tib-Molbiol (Berlin ...
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bioRxiv - Microbiology 2024Quote: ... 10μl /well cell proliferation reagent WST-1 (Roche 05015944001) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-HA (12CA5 mouse monoclonal antibody, Roche, 1:10.000), anti-Ubiquitin (P4D1 mouse monoclonal antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 x EDTA free proteinase inhibitor (Roche, Indianapolis, IN)) and incubated for 30 minutes on ice ...
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bioRxiv - Developmental Biology 2019Quote: ... 100 µl 10% SDS, 500 µl 20% N-Lavroyl sarsosine sodium, 2 tablets of cOmplete ULTRA Protease Inhibitor Cocktail [Roche Cat.# 05892970001] in 10ml FA buffer). The sample was then sonicated using a Covaris S220 at the following settings ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 µl 10% SDS, 500 µl 20% N-Lauroyl sarsosine sodium, 2 tablets of cOmplete ULTRA Protease Inhibitor Cocktail [Roche Cat.# 05892970001] in 10ml FA buffer). For each IP experiment ...
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bioRxiv - Cell Biology 2020Quote: ... A 2ml Dounce homogenizer was used to individually homogenize each brain in a 1:1 solution of Lysis-R:2X-RIPA buffer solution with protease inhibitors (Roche cOmplete tablets #11836153001). Each sample was sonicated three times for 7 seconds and then centrifuged at 5000g for 5min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were harvested and resuspended in binding buffer (100 mM Tris- Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, complete protease inhibitor cocktail (Roche; 1 tablet/10 mL) before sonicating on ice ...
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bioRxiv - Biochemistry 2021Quote: Bacteria were resuspended in 150 μL lysis buffer (1% Triton X100, 0.5% SDS, 1 tablet cOmplete EDTA-free protease inhibitor cocktail (Roche Diagnostics GmbH, Mannheim, Germany) in 10 mL PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... and sequentially incubated for 1 hour in a serum-based blocking solution and then for 1 hour in blocking solution containing the anti-digoxigenin (Roche, 11093274910; 1/2000) or anti-Fluorescein (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol, supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail) for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The final pellet containing the chromatin fraction was resuspended in lysis buffer (10 mM HEPES, 500 mM NaCl, 1 mM EDTA, 1% NP-40, supplemented with protease and phosphatase inhibitor cocktails, Roche, and Benzonase, Millipore), sonicated 3 times at low amplitude ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl, 5 mM EDTA, 0.5% SDS, 0.5% SDO, 1% Triton X-100, 1 mM PMSF, Roche cOmplete Mini Protease Inhibitors 1x), and homogenized (Precellys Evolution ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membrane was blocked with 5% skim milk in TBS for 1 h and incubated with anti-HA-HRP (Roche, 3F10, 1:5000) in 5% milk/TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% Glycerol, 1 % Triton-X-100, 1.5 mM MgCl2, 1 mM EGTA, 10 mM Pyrophosphate, 100 mM NaF, Roche protease inhibitor cocktail 1x), and protein level quantified using the Pierce BCA Assay Kit (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... the cells were washed with cold 1X-PBS prior to collection with 800μL of chilled lysis buffer (50mM TRIS-HCl [pH=7.4]; 150mM NaCl; 1mM EDTA; 1mM CaCl2; 1mM MgCl2; 1% Triton, 0.5% sodium deoxycholate; protease inhibitor cocktail [Roche] 1 tablet/10mL lysis buffer). The cells were incubated on ice for 30 minutes and then centrifuged at 13,000 rpm/ 15minutes/ 4° C ...
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bioRxiv - Neuroscience 2020Quote: ... 40 mM KCl, 5 mM EGTA, 5 mM MgCl2, 5 mM DTT, 1 mM PMSF, 1% Triton X, protease inhibitor Roche complete, pH 7.2) and sonicated at low power for 5 s ...
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bioRxiv - Cancer Biology 2021Quote: ... 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol, supplemented with 100 U/mL SUPERase-IN and 1X Roche protease inhibitor cocktail) for 10 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: GST-tagged CBX8 was purified by re-suspending thawed cell pellets in 30 ml of lysis buffer (1× PBS, 5 mM DTT, 1× EDTA free protease inhibitor cocktail (Roche Diagnostics, Indianapolis, IN)) per liter of culture ...
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bioRxiv - Biochemistry 2021Quote: ... The pellets of harvested bacteria were resuspended in 5 mL of lysis buffer (20mM NaP pH 7.5, 300mM NaCl, 15mM imidazole, 5% glycerol, 0.5mM TCEP, 1 mg/ml lysozyme, 5 U/ml DNase, 1 Roche protease inhibitor tablet/100mL) per 1 g of wet weight culture ...
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bioRxiv - Biochemistry 2021Quote: ... The pellets were resuspended in 5 mL of lysis buffer (20mM NaP pH 7.5, 300mM NaCl, 15mM imidazole, 5% glycerol, 0.5mM TCEP, 1 mg/ml lysozyme, 5 U/ml DNase, 1 Roche protease inhibitor tablet/100mL) per 1 g of wet weight culture ...
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bioRxiv - Microbiology 2020Quote: NUP153/NSP1-expressing cell pellets were resuspended in lysis buffer (1× PBS, 0.5 mM TCEP, 0.1 mM PMSF, 1× Roche cOmplete protease inhibitors, pH 7.4), and lysed in a homogenizer ...
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bioRxiv - Genetics 2019Quote: ... Sheered chromatin samples were pre-cleared by incubating with the 250ul aliquot of Dynabeads resuspended in 2ml of Dilution Buffer (20mM Tris pH=8, 2mM EDTA, 150mM NaCl, 1% Triton X-100, 1:50 Roche cOmplete protease inhibitor) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were spun down and washed twice in 1 mL of buffer (50 mM Hepes, pH 7.4, 150 mM NaCl, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001)) and subjected to sample preparation for MS measurement ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM NaCl, 1.5 mM MgCl2, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) with 100 U/ml of OmniCleave nuclease (Epicentre ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% Triton X-100, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail), incubated for 20 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% IGEPAL Nonidet P-40, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) and transferred to a Dounce homogenizer and homogenized with 20 slow strokes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in 10 ml of extraction buffer (pH 6.7) (250 mM sucrose, 120 mM KCl, 10 mM MOPS, 5 mM MgCl2, 1 mM DTT, 1 Roche protease inhibitor cocktail tablet) (Vought et al ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 mL of buffer 1 (20 mM K-HEPES pH 7.9, 50 mM KCl, 10% glycerol, and Roche EDTA-free protease inhibitors). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... 1% Triton X-100, 10% glycerol, 10 mM NaH2PO4, 10 mM NaP2O7, 100 μM ZnCl2, 10 mM NaF, 1 Roche protease inhibitor tablet), lysed by sonication and the supernatant isolated by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche], and PhosStop [Roche]; pH 7.5) and sonicated for 30 s ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were lysed in lysis buffer (50 mM Tris/HCl, 1 mM EDTA, 1% SDS, phosphatase inhibitor cocktail (Roche, Indianapolis, IN, USA), and protease inhibitor cocktail (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse IgG1 mAb α-GFP (1:500, Roche Diagnostics #11814460001), mouse IgG2 mAb α-Centrin clone 20H5 (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated overnight at 4°C with anti-Digoxigenin-AP-conjugated antibody (dilution range: 1:1000 to 1:5000, Roche, catalog no. 11093274910) diluted in the same buffer ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8, 150 mm NaCl, 1 mm EDTA, 1% Triton X-100, and cOmplete™ EDTA-free Protease Inhibitor Mixture, Roche Applied Science) before clarification by centrifugation and enrichment for FLAG-tagged proteins using anti-FLAG® M2 Affinity Gel (A2220 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 M NaCl, 30 mM imidazole, 1 mM DTT, 1 mM benzamidine, and one EDTA-free complete protease inhibitor cocktail tablet [Roche] per 50 ml). The lysate was clarified by centrifugation at 38,000 g in a fixed-angle rotor ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 1 mM EDTA, 1% v/v Triton X-100, 10% v/v glycerol, 1x cOmplete protease inhibitor cocktail [Roche], and 1x PhosSTOP [Roche]). Cells were scraped off ...
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bioRxiv - Developmental Biology 2020Quote: ... expressing HA-tagged TWIST1 interaction candidates were thawed in 300 μL hypotonic lysis buffer (HEPES 20 mM, MgCl2 1 mM, Glycerol 10%, Triton 0.5 %. DTT 1 mM, 1x Complete protease inhibitor [Roche], Benzo nuclease 0.5 μl/ ml) and incubated at room temperature for 15 mins (for nuclease activity) ...
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bioRxiv - Genetics 2020Quote: ... 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml, ½ Roche phosSTOP tablet/10 ml) using 14 × 1.5 min bursts on a BioSpec mini bead-beater at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were thawed in one pellet volume of RP buffer (50 mM Tris pH 8.0, 300 mM NaCl, 1 mM MgCl2, 1 mM PMSF, Roche Complete protease inhibitor tablet 1x). Cells were opened using a French press (Constant Systems LTD ...
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bioRxiv - Neuroscience 2020Quote: ... for 30 min at 4°C on a rotator and S1 (1 ml for cerebellum and 1 ml for cerebrum) was incubated with 5 μg of anti-HA mAb (Roche, clone 12CA5, RRID: AB_514505) at 4°C for 60 min on a rotator ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated for at least 1 h with a 1:1000 dilution of a high affinity anti-HA antibody from rat IgG1 (#11867423001, Roche Applied Science, Penzberg, Germany). Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795 ...
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 10 mL of L2 buffer (200mM NaCl 1mM EDTA pH 8 0.5mM EGTA 10mM Tris, pH 8, dH2O, 1 protease inhibitor tablet (Roche Complete cat #1 697 498) per 50ml buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... and subsequently incubated in antibody solution (5% Sheep serum, 1% Tween20 and 1:2500 dilution of Alkaline Phosphatase-linked α-Digoxigenin antibody, Roche, 11093274910 in TBST) for 2 h at RT ...
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bioRxiv - Plant Biology 2020Quote: ... Primary antibodies used were αHA-HRP 1:2000 (12013819001 Roche), αHY5(N ...
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bioRxiv - Plant Biology 2020Quote: ... 1 mM phenylmethylsulfonyl fluoride (PMSF) and protease inhibitor cocktail (Roche)) ...