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Citations for Roche :
4201 - 4250 of 8835 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... cells were incubated in a 1:1000 dilution of 1 mg/ml DAPI (Roche Diagnostics) in PBS for 5 min followed by three washes with PBS ...
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bioRxiv - Cell Biology 2024Quote: EV preparations were lysed using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche) and EV concentration was determined using Pierce™ MicroBCA protein assay kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was discarded and the pellet was resuspended in a 1:1 solution of 100 mM Tris-HCl pH 7.4 with EDTA-free protease inhibitor cocktail (Roche, 1 tablet in 10ml of buffer) and 4x NuPage LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were lysed in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Nonidet P-40, 01% SDS, 1 mM EDTA, supplemented with protease inhibitor cocktail [Complete Roche Mini, 1 tablet per 50 ml]) and spun at 20.000 g to eliminate cell debris ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT) supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg ml−1 lysozyme and a tablet of complete EDTA-free Protease Inhibitor Cocktail (Roche). Cells were lysed by sonication on ice for 3 min and centrifuged at 48,300 × g for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were reverse transfected with 1:3 or 1:6 X-tremeGENE 9™ (Roche) transfection reagent according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... for 1 hr at RT and incubated overnight with anti-Digoxigenin-AP (1:4000, Roche 11093274910) in Blocking Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Blocking of membranes was carried out in buffer 2 (buffer 1 plus 1% blocking reagent (Roche)) for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mg/ml bromophenol blue) and 1 μl proteinase K (14–22 mg/ml, Roche). The reaction was further incubated for 15 mins at 30°C for deproteination ...
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bioRxiv - Neuroscience 2023Quote: ... DRG were incubated for 1 hour at 37 °C with 1 mg/ml dispase II (Roche) and 2 mg/ml collagenase A (Roche ...
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bioRxiv - Microbiology 2023Quote: ... 1% Triton X-100 & 1% Deoxycholate) supplemented with a protease mixture inhibitor (Roche Complete, EDTA free). Lysates were either immunoprecipitated using GFP-trap agarose beads (ChromoTek ref# gta-10 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with DAPI (1/2000 in sterile PBS from 1 mg/ml stock, Roche). Sorted cells were re-suspended in medium to reach a 2.5 million cells/ml concentration ...
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bioRxiv - Biochemistry 2024Quote: ... 35 mM imidazole) + protease inhibitors (1 tablet / 50 ml Roche Complete EDTA-free, 1 mM AEBSF), lysozyme ...
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bioRxiv - Genomics 2024Quote: ... Miniaturization involved testing libraries at 1/6th (IDT mini) or 1/8th (Roche mini, Illumina mini) of reaction volume tailoring input DNA to each kit between 24-45 ng total (Table 2) ...
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bioRxiv - Microbiology 2023Quote: ... 1% sodium deoxycholate and 1% Triton X-100) supplemented with Complete protease inhibitors without EDTA (Roche). Cellular debris was removed by centrifugation at 17,000 × g ...
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bioRxiv - Microbiology 2023Quote: ... at a 1:1 ratio in a 96-well deep-well extraction plate (Roche Diagnostics GmbH), covered with a MagNA Pure Sealing Foil (06241603001 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% NP40 substitute) with complete protease inhibitor cocktail (Roche Cat. #11836145001; 1 tablet per 50 mL). Samples were homogenized twice for 30 seconds at 6.5m/s in a fast-prep machine with an incubation at 4 °C for 5 min between runs ...
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bioRxiv - Genetics 2022Quote: ... 10 ul of Protease Inhibitor Cocktail (Roche; 1 tablet diluted in 1 ml of DEPC H2O), and 0.5 ul of Ribolock) ...
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bioRxiv - Developmental Biology 2022Quote: ... and then blocked in TNT/block [0.5% blocking reagent (PerkinElmer FP1012)/TNT] for 1 hour at RT before ON incubation with anti-DIG (1:100, Roche 11207733910) or anti-Fluo (1:250 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM TCEP) supplemented with Protease Inhibitor Cocktail (1 tablet/30 ml; complete, EDTA-free, Roche) and sonicated on ice ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium deoxycholate) supplemented with 1 mM PMSF and 1× protease inhibitor cocktail (Roche; Cat# 04693132001). The cells were broken using Zirconia/Silica beads (BioSpec ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution was neutralized with a PBS-diluted protein inhibitor cocktail (cOmplete, EDTA-free, protease inhibitor cocktail tablets: Roche Diagnostics GmbH, Mannheim, Germany) and used to infect electroporation-competent cells ...
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bioRxiv - Cell Biology 2020Quote: ... 50 μg of proteins from the microsomal membrane pellet (P13) were incubated with 30 μg/ml of proteinase K (Roche Diagnostics, Mannheim, Germany) for 30 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... were used to remove salts from purified protein and to exchange to freezing buffer (20mM HEPES, 100mM NaCl, 0.5mM EDTA, TCEP 0.5mM and Roche cOmplete™ Protease Inhibitor Cocktail), using the gravity protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue extracts were subjected to TCA precipitation as previously described53.Washed protein pellets were solubilized in 900 μl of urea buffer (8M urea, 150mM NaCl, 50mM Tris pH8, 1X Roche complete protease inhibitor). Protein quantitation was performed using Qubit fluorometry (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... The elute was neutralized with a PBS-diluted protein inhibitor cocktail (cOmplete, EDTA-free, protease inhibitor cocktail tablets: Roche Diagnostics GmbH, Mannheim, Germany) and used to infect TG1 cells ...
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bioRxiv - Cell Biology 2020Quote: ... HA-Kir6.2 was detected with HRP-conjugated rat anti-HA antibody (Roche, 3F10, 0.005 μg/ml). GFP was detected with mouse anti-GFP antibodies (AbCam ...
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bioRxiv - Evolutionary Biology 2021Quote: ... labelled riboprobes were detected using anti-digoxigenin or anti-fluorescein antibodies conjugated to horseradish peroxidase (Roche). Fluorescent tagging was performed using Alexa Fluor 594 and 488 dyes with streptavidin Tyramide Signal Amplification (TSA ...
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bioRxiv - Neuroscience 2021Quote: ... Post hoc immunostaining was performed using rat anti-HA primary antibodies (Roche, #11 867 423 001) and anti-rat Alexa568 (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: Commercially available primary antibodies used for western blot are rat anti-HA tag (clone 3F10; Roche), rabbit anti-Cyclin B1 (4138S ...
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bioRxiv - Microbiology 2021Quote: ... Dilutions for primary antibodies were: mouse monoclonal (mixture of clones 7.1 and 13.1) anti-GFP (Roche), 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Dig was detected using anti-Dig antibody conjugated with alkali phosphatase and CDP-Star substrate (Roche) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (0.5 mg) were incubated with anti-HA antibody-coupled agarose beads (Roche, Basel, Switzerland) for 16 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... the southern blot was detected by using an anti-digoxygenin antibody coupled to alkaline phosphatase (Roche) and the labeling and detection starter kit (Roche).
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bioRxiv - Cell Biology 2022Quote: ... Samples were analysed by western blotting using a α-GFP antibody (Roche, 11 814 460 001), and a α-Pgk1 antibody (Invitrogen ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Blocking buffer was then removed and a peroxidase-conjugated anti-DIG antibody (anti-DIG-POD, Roche) was added at 1:2000 in 1% BSA in T-TBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Visualization of the labeled probe was done using anti-DIG-AP (alkaline phosphatase) antibodies (Roche Biochemicals) followed by histochemical enzymatic staining reagents (Roche Biochemicals) ...
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bioRxiv - Developmental Biology 2022Quote: ... Whole-mount two-color fluorescence ISH was performed using anti-DIG and -fluorescein POD antibodies (Roche) and Tyramide Signal Amplification (TSA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After the reaction between DIG-labeled RNA and anti-DIG antibody conjugated horseradish peroxidase (HRP) (Roche), sections were washed 3 times by TNT buffer (100 mM Tris-HCl ...
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bioRxiv - Genetics 2020Quote: ... the membrane was blocked with blocking buffer and incubated with anti-DIG antibody (Roche detection kit), washed (maleic acid ...
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bioRxiv - Biophysics 2021Quote: ... Monoclonal cell lines were identified by immunocytochemistry using a rat anti-HA antibody (Roche Applied Science). HEK293 cells stably overexpressing rat sACt were as described (58).
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bioRxiv - Developmental Biology 2023Quote: ... Detection was done with alkaline phosphatase-labeled anti-DIG antibody and NBT/BCIP (Roche diagnostics, USA). Control experiments with sense probes did not give detectable signals (data not shown) ...
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bioRxiv - Neuroscience 2023Quote: ... PH 7.5)) and then incubated overnight at 4 °C in anti-DIG POD antibody (Roche, 11207733910) diluted 1:300 in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were probed with the following primary antibodies: rat α-HA (clone 3F10; Roche Applied Sciences), rabbit α-Myc (Cell Signaling Technology) ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... The antibodies used in immunoprecipitation were as follows: mouse anti-GFP (clones 7.1 & 13.1; 11814460001, Roche) and mouse anti-HA (F-7 clone ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... was incubated with 1.5 μg of GFP antibody (mouse anti-GFP, clones 7.1 & 13.1; 11814460001, Roche), for 2 h on a rotating wheel at 4 °C ...
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bioRxiv - Physiology 2024Quote: ... Hybridized probes were detected using an alkaline phosphatase-conjugated anti-DIG antibody (RRID: AB_514497; Roche Diagnostics) with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP ...
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bioRxiv - Physiology 2024Quote: ... and incubated overnight with the alkaline phosphatase (AP) coupled anti-digoxigenin or anti-fluorescein antibody (Roche) at respectively 1/5000 or 1/1000 in blocking buffer ...