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Citations for Roche :
4201 - 4250 of 8559 citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... resuspended with precooled 1 mL isotonic sucrose buffer (290 mM sucrose, 10 mM imidazole, pH 7.0, 1 mM DTT, and 1 cOmplete protease inhibitor cocktail (Roche) per 10 mL buffer ...
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bioRxiv - Cell Biology 2022Quote: ... BRB80 buffer (80 mM Pipes pH 6.8, 1 mM MgCl2, 1 mM EGTA) supplemented with 1X protease inhibitors (Roche), and their ovaries were extracted with pre-cleaned forceps ...
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bioRxiv - Cell Biology 2022Quote: ... A single-cell solution was obtained by tissue digestion using 1mgml−1 collagenase D and 0.1mgml−1 DNase I (Roche) in HBSS (Lonza ...
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bioRxiv - Immunology 2022Quote: ... Cells were spun down again and the pellet resuspended in 1.25 ml cold lysis buffer (PBS containing 1% TX-100, 1 mM NEM, and Complete Protease Inhibitor from Roche). Cells were lysed for 30 minutes on ice ...
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bioRxiv - Cell Biology 2021Quote: ... mitochondria were lysed in lysis buffer (50 mM Tris pH 7.4, 100 mM KCl, 1 mM EDTA, 1× Complete Protease Inhibitor cocktail (Roche), 1mM PMSF ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5, 1 mM EGTA, 10% sucrose, 0.8 M NaCl containing sarkosyl (final 1%, w/v) and protease inhibitor (Roche). After ultracentrifugation at 135,000 × g for 20 min at 25 °C ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed with lysis buffer (final concentration: 50 μg ml−1 lysozyme, 0.8% NP-40, 1 × protease inhibitor (Roche), 250 U ml−1 benzonase ...
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bioRxiv - Genetics 2019Quote: Cells were lysed with modified RIPA (50 mM Tris-HCL, 1% NP40, 0.25% Na-deoxycholate, 150 mM NaCl, and 1 mM EDTA; CompleteTM Protease Inhibitor Cocktail [Roche]), and protein concentrations were determined with the BCA-Kit (Pierce) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... cells were lysed with 100 µl Lysis buffer (10 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM EDTA, 1% Triton X-100, Protease Inhibitor Cocktail Roche). The cell pellet was resuspended by pipetting and incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mM Na3VO4 and supplemented with a protease inhibitor cocktail (1 tablets to 10 ml of lysis buffer, Roche) for 30 minutes at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... in a 1:1 volume ratio of Tris-buffered saline (pH 8.0) with added proteinase inhibitors (EDTA-free; Roche). Sample homogenization was confirmed by microscopic inspection ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.1% SDS, 1% deoxycholic acid, 0.5 mM PMSF, 1 mM DTT, 0.1 mM sodium orthovanadate, and Roche protease inhibitors). Nuclear lysates were sonicated with a Branson 250 Sonifier (output 20% ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl; 10 mM EDTA; 0.1% SDS; 1% Triton X-100; 1% deoxycholate; 1x complete protease inhibitor cocktail [Roche]), clarified and 20 μg total protein were used for SDS-PAGE followed by electroblotting ...
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bioRxiv - Developmental Biology 2020Quote: ... Whole cells extract was isolated using RIPA buffer (NaCl 150mM – Tris HCL pH7,35 50mM – DOC 1% – N-P40 1% – H2O) supplemented with protease inhibitor (04 693 116 001, Roche). Isolated protein concentration was determined using Bradford assay (500-0006 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.1% NP-40, and a protease inhibitor cocktail tablet [Roche]). After clarifying lysates by centrifugation at 2,000 rpm for 1 min at 4 °C twice ...
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bioRxiv - Neuroscience 2022Quote: ... previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5, 150 nM NaCl, 1% NP-40, 1x protease inhibitors (Roche)) for 3 times ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ml urea buffer (8M urea, 1mM EDTA, 10mM TrisHCl pH 7.5, 1 mM PMSF, 1x Roche protease inhibitor) was then added and the sample was gently mixed ...
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bioRxiv - Cancer Biology 2022Quote: ... whole-cell extracts were prepared by lysing cells in buffer X (50 mM Tris pH 8.5, 250 mM NaCl, 1 mM EDTA, 1% NP-40, protease inhibitor minitablet (Roche)) and quantified using Bradford assay (Bio-Rad) ...
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bioRxiv - Biophysics 2022Quote: ... Frozen bacteria containing full-length or ΔUBLΔUBA UBQLN constructs were resuspended in Buffer A (50 mM Tris pH 8, 1 mM MgCl2, 1 mM PMSF, and 0.2 mg/mL DNase I and Roche Complete Mini protease inhibitor cocktail) ...
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bioRxiv - Genomics 2022Quote: ... before being re-pelleted by centrifuging for 3 minutes at 4°C at 500g and resuspended in 180µl TMS buffer (10mM Tris-HCl pH 8, 1mM MgCl2, 1% SDS, 1× Complete Protease Inhibitor Cocktail (PIC, Roche)) with 0.4µl benzonase (E1014 ...
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bioRxiv - Genetics 2022Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, Complete EDTA-free protease inhibitor cocktail tablets, Roche) with half volume of glass beads (Sigma ...
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bioRxiv - Immunology 2022Quote: ... the cells were lysed with ChIP-Lysis buffer (10 mM EDTA, 1% [w/v] SDS, 50mM Tris-HCl, pH 7.5, 1 tablet Protease Inhibitor Cocktail [#11836170001; Roche, Mannheim ...
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bioRxiv - Plant Biology 2022Quote: ... 150mM NaCl, 1mM EGTA, 50mM HEPES, pH 7.5, 1 mM PMSF and 1× cOmplete protease inhibitor cocktail from Roche). 30 μl GST beads (Glutathione Sepharose 4 Fast Flow ...
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bioRxiv - Plant Biology 2022Quote: The samples were ground in liquid nitrogen and homogenized in the extraction buffer (100 mM Tris-HCl pH 8.8, 150 mM NaCl, 1 mM EDTA, 20% glycerol, 1 mM PMSF, 1x cOmplete protease inhibitor cocktail from Roche, 1x phosphatase inhibitor cocktail 2 and 3 from Sigma) ...
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bioRxiv - Systems Biology 2022Quote: ... 6 mM MgCl2, 1 mM EDTA, 100 mM NaCl, 0.1% Tx-100, pH 8, and 1× Protease Inhibitor Cocktail, Roche) at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was transferred to a blocking buffer (1 M maleic acid solution pH7.4, 1% nucleotide blocking reagent (Roche)) for 30 minutes and then incubated in antibody-solution (anti-dioxigenin-AP fragments in blocking buffer) ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to the indicated samples in Pull-down Buffer 1 containing 1% (w:v) BSA supplemented with cOmplete EDTA-free protease inhibitor cocktail (Roche) (final volume ...
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bioRxiv - Molecular Biology 2022Quote: ... then resuspended in cytoplasmic lysis buffer (25 mM Tris–HCl pH 7.5, 10 mM NaCl, 1.5 mM MgCl2, 1% IGEPAL CA-630, and 1× protease inhibitor cocktail [“PI”, Roche 11836170001]) at 4°C and incubated on ice for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 0.5% Triton X-100, 1 mM DTT, 150 μM ZnSO4, 1 mM PMSF, 1xEDTA-free protease inhibitors [Roche]) overnight on a rotating wheel at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Remaining red blood cells were resuspended in extraction buffer (50 mM Tris [pH8.0], 1 mM EDTA [pH8.0], 0.5% SDS and 1 mg / ml Proteinase K [Roche, Nutley, NJ]) and incubated overnight at 55°C ...
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bioRxiv - Developmental Biology 2024Quote: ... the sections were washed in a serial SSC buffer and formamide solution and then preincubated in buffer 1 (100 mM Tris-HCl, pH 7.5, 150 mM NaCl) with a 1% blocking reagent (Roche) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated for 1 h at room temperature (20– 25 °C) in 0.1 M PBS with 1% blocking reagent (Roche) and 0.3% Tween 20 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were harvested and washed thrice with FACS washing buffer (1X PBS + 1 mM EDTA, pH 7.2 + 1 Complete Inhibitor EDTA-free tablet (Roche) per 50 mL buffer) ...
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bioRxiv - Immunology 2024Quote: ... followed by tissue digestion for 1 h at 37°C with Iscov’s digestion media containing 1 mg/ml Liberase TL (Roche), and 0.5 mg/ml DNAse (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 50 µg of cryoground tissue powder was placed in a 1.5mL lobind tube and brought to 1 mL with lysis buffer (1% triton, 1x PBS, 1x Roche Complete protease inhibitor cocktail ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 1 ml cytoplasmic lysis buffer (25 mM Tris–HCl pH 7.5, 10 mM NaCl, 1.5 mM MgCl2, 1% IGEPAL CA-630, and 1× protease inhibitor cocktail [“PI”, Roche 11836170001]) at 4°C ...
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bioRxiv - Genomics 2022Quote: ... and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG, RRID:AB_262044 or monoclonal mouse anti-GFP, 1:1,000, Roche #11814460001, RRID:AB_390913) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were prepared by resuspending cell pellets in 2 mL NP-40 buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 1% NP-40, 1 mM PMSF, 1x PhosSTOP [Roche]), sonication on ice (5s on/10s off for 2 mins at 40% amplitude) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was digested for 45-60’ at 37°C in a Thermomixer at 800 rpm in 1 mL of 1× PBS + 0.5 mM CaCl2 + 250 μg/mL Liberase (Roche) + 2 mg/mL of Collagenase (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse atria were minced and digested with mild agitation for 30 minutes at 37°C in DMEM containing 1% P/S and 0.14 Wunsch U ml-1 Liberase (5401119001, Roche). MAFs were enriched via negative selection with magnetic beads against mouse CD45 (leukocytes) ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial pellets were solubilized in lysis buffer with 1 % digitonin (25 mM HEPES pH 7.5, 150 mM NaCl, 5 % glycerol, 1 mM TCEP, supplemented with PhosStop (Roche), cOmplete protease inhibitor cocktail EDTA-free (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was washed twice with 300 μl sodium phosphate buffer (20 mM Na-phosphate pH 6.8, 1 mM PMSF, 1× Mini Protease Inhibitor Cocktail, Roche) with centrifugation at 16,000g for 20 min between washes ...
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bioRxiv - Molecular Biology 2023Quote: ... the cell pellet was resuspended in 10 mL of column buffer (20 mM Tris-HCl pH 7.5, 200 mM NaCl, 1 mM EDTA, protease inhibitors [1 Roche Mini EDTA-free tablet] ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in lysis buffer (50 mM PBS pH 7.4, 1 mM EDTA, 5% glycerol, 1 mM PMSF and protease inhibitors from Roche). Cell lysis and protein extraction was achieved through mechanical lysis using glass beads and Precellys®24 Homogenizer (3 cycles of 30 seconds at 5500 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... 140 mM NaC1, 1 mM EDTA, 10% glycerol, 0.1% NP-40, 1 mM PMSF, and 1x protease inhibitor cocktail [Complete; Roche]). The cell pellet was flash frozen in liquid nitrogen with 1.5 mL of lysis buffer and grounded in a Spex Freezer Mill 6775 to a fine powder ...
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bioRxiv - Microbiology 2023Quote: 293T cells transfected with pCAG AcGFP-FOS or pCAG AcGFP-JEVcore-FOS were incubated for 48 h and lysed in lysis buffer (20 mM Tris-HCl [pH = 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche]). The cell lysates were incubated with Strep-Tactin Sepharose (IBM GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in a 1:1 ratio (w/v) in PB supplemented with Complete Protease Inhibitor Cocktail (Roche) and afterward dropwise frozen in liquid nitrogen before lysed in 6875D LARGE FREEZER/MILL (SPEX SamplePrep LLC) ...
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bioRxiv - Immunology 2023Quote: ... were lysed in 1 mL lysis buffer (1% IGEPAL-CA 630, 300 mM NaCl, 100 mM Tris pH 8.0, 1X Roche cOmplete Mini Protease Inhibitor Cocktail ...
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bioRxiv - Plant Biology 2024Quote: ... were ground in liquid N2 and 100 µL extraction buffer added (125 mM Tris-Cl pH 8.8, 1% SDS, 10% glycerol, 1 mM PMSF and Complete Protease Inhibitor [Roche]). Samples were centrifuged at 7000 g for 10 min and supernatant collected ...