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Citations for Roche :
4051 - 4100 of 7005 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were collected by scraping in TNE buffer containing protease inhibitors (Roche) and pelleted by centrifugation at 0.4xg at 4°C for 5 min (5415R ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed with 1mL of RIPA with protease inhibitor cocktail (Roche), and lysate and media samples were subjected to immunoprecipitation with G1 Anti-DYKDDDDK affinity resin overnight at 4°C while rocking ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed with 1mL of RIPA with protease inhibitor cocktail (Roche), and lysate and media samples were subjected to immunoprecipitation with G1 Anti-DYKDDDDK affinity resin overnight at 4°C while rocking ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed in RIPA buffer containing 10% protease inhibitor cocktail (Roche) for 30 min on ice ...
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bioRxiv - Bioengineering 2024Quote: ... and dissociated into single-cell suspension with DMEM containing DNase (Roche, 10104159001) and collagenase (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Upon adherence of calvarial osteoblasts or OCY454 cells on Collagen I (Roche)-coated surfaces of 8-well chambers ...
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bioRxiv - Molecular Biology 2024Quote: Cells were lysed by RIPA (Beyotime) supplemented with Protease Inhibitor Cocktail (Roche), PMSF (Sangon ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells were transfected with X-tremeGENE HP DNA transfection agent (Roche) with a 1:2 DNA:reagent ratio and incubated for 24 – 48 hours before continuing with other experiments.
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bioRxiv - Immunology 2024Quote: ... and the cells were incubated with 10 % WST reagent (Roche, Basel, Switzerland) for 15 min at 37 °C and 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell pellets were resuspended in PBS supplemented with 1x Protease Inhibitor (Roche) and 50U of Benzonase (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed using RIPA buffer supplemented with protease inhibitors (Roche 11873580001) per manufacturer’s instructions on ice for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: Cells were lysed in RIPA buffer containing phosphatase and protease inhibitors (Roche) and spun to remove insoluble debris ...
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bioRxiv - Neuroscience 2024Quote: ... 25 μl cell proliferation reagent WST-1 (Roche, Cat. No. 05015944001, Switzerland) was added to each 500 μL/well serum-free DMEM/F12 (0.5:10 dilution) ...
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bioRxiv - Biochemistry 2024Quote: ... was transiently transfected into the cells using XtremeGene HP transfection reagent(Roche). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2024Quote: Approximately 20,000 endogenously tagged HEK293T cells were grown on a fibronectin (Roche)-coated 96 well glass bottom plate (Cellvis ...
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bioRxiv - Cancer Biology 2024Quote: Subconfluent cells were harvested in RIPA buffer containing protease (cOmplete, Roche 11836153001) and phosphatase (PhosSTOP ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed in 1% Triton X-100 with protease inhibitor (Roche). Lysates were centrifuged for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... pellet was resuspended in red blood cell (RBC) lysis buffer (Roche, 11814389001) and incubated for 90s at room temperature before stopping the lysis with 6 ml of PF10 ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates were collected using buffer containing protease inhibitors (Roche, Basel, Switzerland) and a phosphatase inhibitor cocktail (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was then amplified using an SYBR green RT-PCR kit (06924204001, Roche Products, Mumbai, India) for a 139 bp region in the Tat exon-1 using the primers- N1783 (5’- GGAATCATCCAGGAAGTCAGCCCGAAAC-3’ ...
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bioRxiv - Biophysics 2021Quote: Cholesterol concentrations were measured calorimetrically using a commercial kit (R-Biopharm and Roche Diagnostics, Cat. No. 10139050035) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labeled RNA probes were synthesized by in-vitro transcription using the DIG RNA labeling kit (Roche). In situ analysis was performed as described previously (Cecchetti et al. ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems, Wilmington, MA) according to the manufacturer’s protocol [39] ...
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bioRxiv - Immunology 2021Quote: ... Quantitative reverse transcription PCR (qRT-PCR) was performed using an SYBR FAST universal qPCR kit (KAPA Biosystems). Predesigned KiCqStart primers for DDX58 ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR (Q-PCR) reactions were performed using LightCycler FastStart DNA MasterPlus SYBR Green I kit (Roche). For each primer set ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using the KAPA SYBR FAST Universal qPCR Kit (KAPA BIOSYSTEMS) in a QuantStudio3 system (Life Technology) ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng of fragmented DNA was used for library preparation using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2021Quote: ... The library was quantified using the KAPA library quantification kit for Illumina sequencing platforms (KAPA Biosystems, KK4824) and 50bp single end reads were sequenced on a HiSeq2500 following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2019) via 15 rounds of high-fidelity PCR amplification (KAPA HotStart PCR Kit, KAPA Biosystems, Wilmington, Massachusetts). Each pooled ...
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bioRxiv - Microbiology 2021Quote: ... ATP concentrations were determined using the ATP Bioluminescence Assay Kit CLS II (Roche 11699695001, from Sigma-Aldrich) according to manufacturer’s instructions ...
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Distal regulation, silencers and a shared combinatorial syntax are hallmarks of animal embryogenesisbioRxiv - Developmental Biology 2021Quote: ... The pool is assessed by qPCR using the KAPA Library Quantification Kit - Illumina/Universal (KAPA Biosystems, KK4824) combined with the Life Technologies Via 7 real-time PCR instrument ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems) and average size fragment was determined using a LabChip GX (PerkinElmer ...
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bioRxiv - Developmental Biology 2022Quote: ... Antisense and sense probes were synthesized by in vitro transcription using a DIG RNA Labeling Kit (Roche). Following labeling ...
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bioRxiv - Physiology 2022Quote: ... using the LightCycler FastStart DNA Master plus SYBR Green I kit (catalog n° 03515869001, Roche, Basel, Switzerland). 40ng of reverse transcribed RNA was used as template for each reaction ...
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bioRxiv - Neuroscience 2021Quote: ... Library QC included size distribution check (BioAnalyser) and concentration determination with KAPA Library Quantification Kit (KK4857, Roche). Libraries were equimolar pooled and loaded on Illumina NovaSeq 6000 SP flowcell at 300 pM loading concentration with 1 % of PhiX by-mix ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was reverse transcribed with a Transcriptor First Strand cDNA Synthesis Kit (Roche Applied Sciences, Indianapolis, IN). Quantitative RT-PCR was performed using Applied Biosystems pre-designed TaqMan Gene Expression Assays for rats and TaqMan® Fast Advanced Master Mix ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA library was prepared for RNA-seq using KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems Inc.). The cDNA libraries were sequenced using Illumina HiSeq machine for paired-end reads using GENEWIZ (South Plainfield ...
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bioRxiv - Molecular Biology 2020Quote: ... The library pool was quantified via qPCR with a KAPA Library-Quant kit (Kapa Biosystems Cat#KK4824). Each sample was split in half and half were sequenced on an Illumina HiSeq 4000 system for single-end 100-bp reads ...
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bioRxiv - Immunology 2021Quote: 300ng of total RNA were reverse-transcribed to cDNA with Transcriptor First Strand cDNA Synthesis Kit (Roche) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Lactate dehydrogenase (LDH) release in the apical medium was measured with the Cytotoxicity Detection Kit (Roche 04744926001) as described previously (36) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and DNA libraries for the remaining samples using the KAPA Hyper Prep kit (KAPA Biosystems, Wilmington, MA). We sequenced paired-end DNASeq and RNASeq reads (2 x 300 ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems). Libraries were pooled in equimolar quantities and sequenced on an Illumina HiSeq 2500 platform using a Rapid Run Mode flowcell following Illumina’s recommended protocol to generate single end reads of 100 bases in length.
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bioRxiv - Genomics 2020Quote: ... Genomic DNA libraries were prepared using the KAPA Hyperplus Library Preparation kit (KAPA Biosystems, Wilmington, MA, US) according to kit instructions ...
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bioRxiv - Genomics 2020Quote: Northern probes were first synthesized using the PCR DIG Probe Synthesis Kit (Roche # 11 636 090 910). The 512 bp frq probe was amplified from wild-type Neurospora genomic DNA with primers ...
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bioRxiv - Molecular Biology 2021Quote: ... PAN specific oligonucleotide probes were labeled with a DIG-Oligonucleotide tailing kit (Sigma Roche 2nd generation 03353583910) and mixed at the 1:1:1 ratio ...
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bioRxiv - Microbiology 2021Quote: ... we used a LightCycler® 480 High Resolution Melting Master (HRMM) kit (Roche; Cat. No. 04909631001, Roche Diagnostics Australia Pty ...
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bioRxiv - Microbiology 2021Quote: ... and the pool was quantified by qPCR using the KAPA Library Quantification Kit - illumina/Universal (KAPA Biosystems) in combination with the Life Technologies Viia 7 real time PCR instrument.
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bioRxiv - Systems Biology 2021Quote: ... After neutralizing the samples with HCl the free fatty acids were assayed using a commercial kit (Roche). Concentrations of cholesterol and acetate were measured using commercial kits supplied by Boehringer Mannheim.
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bioRxiv - Biochemistry 2021Quote: ... RNA-Seq libraries were prepared using the Kapa mRNA HyperPrep RNA-seq library construction kit (Kapa/Roche), with 6 min fragmentation at 94 °C and nine PCR cycles of final amplification and duplex barcoding ...