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Citations for Roche :
4051 - 4100 of 5179 citations for Human Suprabasin SBSN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... the entire miniprepped volume was used as template in a PCR reaction using the KAPA HiFi PCR Kit (Kapa Biosystems). A ten-fold dilution of this PCR product was used as template in a barcoding PCR adding Illumina adapter sequences and dual barcodes ...
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bioRxiv - Microbiology 2019Quote: ... And 1.0 μg total RNA was reverse-transcribed into cDNA using the transcriptor first-strand cDNA synthesis kit (Roche, China). qPCR was conducted using FastStart Universal SYBR Green Master (Rox ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The cDNA encoding for the A33 construct was obtained by total RNA extraction from the HT-29 colorectal carcinoma cell line (High Pure RNA Isolation Kit, Roche), reverse transcription (Transcriptor reverse transcriptase ...
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bioRxiv - Genomics 2021Quote: ... were used to prepare single-end (SE) libraries with an insert size of 300 bp using a PCR-free workflow with the KAPA HyperPrep Kit (Roche) following manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The library was prepared by the Genome Technologies core facility at JAX using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... TUNEL staining was performed on testis sections according to the instructions provided with the cell death detection kit (11684795910, Roche). To reduce experimental variations ...
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bioRxiv - Developmental Biology 2021Quote: ... Template DNAs of interest (500 ng each) were transcribed in vitro during 2 hours at 37°C using SP6 RNA polymerase kit (Roche), followed by a 1 hr treatment with DNAse I (Roche ...
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bioRxiv - Immunology 2021Quote: Strand-specific ribosomal RNA depleted RNA-seq libraries were prepared from 1 µg of total RNA using the KAPA Stranded RNA-seq Kit with Ribo-Erase (Roche). Samples were prepared in at least triplicate for analysis ...
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bioRxiv - Microbiology 2021Quote: ... The 32P-radiolabeled probe was generated from a plasmid containing the entire HPV16 genome by radiolabeling using a Random Prime labeling kit (Roche).
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bioRxiv - Neuroscience 2020Quote: ... Pooled libraries were cleaned using a 0.6:1 ratio of Ampure beads and quantified on Qubit using HS DNA reagents and with the KAPA Library Quantification Kits for Illumina (Roche). Samples were sequenced on two separate rapid-runs on HiSeq2500 (Illumina) ...
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bioRxiv - Developmental Biology 2021Quote: ... were generated cloning each enhancer from the genomic zebrafish DNA DNA using KAPA Long Range HotStart PCR kit (Kapa Biosystems) and cloned into the E1b:GFP:AC-DS vector (102417 Addgene (Chong-Morrison et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Further quantification of the adapter ligated fragments and confirmation of successful P5 and P7 adapter incorporations were assessed with the KAPA universal library quantification kit for Illumina (Roche) run on a ViiA7 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: The RNA-Seq libraries were prepared from total RNA using KAPA Stranded mRNA-Seq Kit Illumina Platforms (Roche-Kapa Biosystems) with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: The RNA-Seq libraries were prepared from total RNA using KAPA Stranded mRNA-Seq Kit Illumina Platforms (Roche-Kapa Biosystems) with minor modifications ...
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bioRxiv - Evolutionary Biology 2020Quote: RNA was extracted from 200 μl swab rectal samples or fecal pellets with the High Pure Viral RNA Kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Viral transport media was added to each pool at a 1:1 ratio for nucleic acid extraction performed on the Roche MagNA Pure 24 platform using the MagNA Pure 24 Total NA Isolation kit (Roche). Elution volume was set to 50 ul to concentrate viral RNA ...
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bioRxiv - Systems Biology 2020Quote: ... strand-specific RNA libraries were prepared using a KAPA Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, catalog no. KK8401) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... was extracted from inactivated samples (200 μL) using the MagNA Pure Compact instrument and MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche Molecular Systems Inc ...
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bioRxiv - Microbiology 2021Quote: ... The tissues were processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159) (Roche Diagnostics Corp. ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then treated with the TUNEL reaction mixture according to the protocol (In situ Cell Death detection Kit, TMR red; Roche) and incubated for 1 h at 37°C in the dark ...
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bioRxiv - Neuroscience 2021Quote: RNA was reverse transcribed using the High Capacity RNA-to-cDNA kit and qPCR performed using a Roche LightCycler480 with SYBR Green I Master Mix (Roche) and the primers listed in Table S2 ...
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bioRxiv - Neuroscience 2020Quote: ... All qPCR reactions were performed in 10 μl volume in triplicates with 1× LightCycler 480 SYBR Green I Master kit (Roche) and primers listed in Supplementary Table 1 on LightCycler® 480 PCR instrument II (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Detection and quantification of apoptosis in cell cultures was determined using a In Situ Cell Death Detection Kit (Roche, 11684795910) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were prepared by the Van Andel Genomics Core from 300 ng of total RNA using the KAPA RNA HyperPrep Kit with RiboseErase (v1.16; Kapa Biosystems). RNA was sheared to 300-400 bp ...
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bioRxiv - Physiology 2021Quote: ... After the concentration of DNA was evaluated by qPCR using the KAPA Library Quantification kit (KAPA Biosystems, Wilmington, MA, USA), the libraries were loaded onto an Illumina MiSeq sequencer and then sequenced using MiSeq Reagent kits v2 500 cycles (Illumina ...
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bioRxiv - Plant Biology 2021Quote: ... the blot was washed at 55°C and probe detection was performed according to the manufacturers’ protocol using the DIG luminescent detection kit (Roche). The intensity of individual bands was measured with respect to the background and integrated density value (IDV ...
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bioRxiv - Bioengineering 2021Quote: ... the probes were detected with anti-digoxigenin and anti-fluorescein antibodies conjugated with alkaline phosphatase (AP) and horseradish peroxidase (HRP) using AP detection kit (Roche) and tyramide signal amplification (TSA ...
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bioRxiv - Microbiology 2020Quote: ... DNA probes for 5S rRNA and SPA0079 were amplified with PCR using primers described in Table S2 and PCR DIG Probe Synthesis Kit (#11636090910, Roche). The reaction was performed with a total volume of 50 μl containing 0.2 μM of forward primer ...
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bioRxiv - Plant Biology 2021Quote: ... first strand cDNA synthesis was performed using mRNA with an oligo-dT primer using Transcriptor High Fidelity cDNA Synthesis Kit (Roche), according to the manufacturer′s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified fragments were then used to produce digoxigenin (DIG)-labeled sense and antisense RNA probes using a DIG RNA Labeling Kit (Roche). Whole-mount in situ hybridization was performed following (39) ...
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bioRxiv - Microbiology 2020Quote: ... gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems) and sequenced on an Illumina NextSeq (High Output ...
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bioRxiv - Plant Biology 2021Quote: ... Gene transcription was quantified by qPCR using a LightCycler 480 SYBR Green I Master kit and LightCycler 480 (Roche, Switzerland). The PCR conditions were 95 °C for 10 min followed by 45 cycles of 95 °C for 10 s ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was reverse-transcribed to cDNA using a Transcriptor First Strand cDNA Synthesis kit (Roche Diagnostics BV, Almere, Netherlands). Viral genomic RNA was quantified by real-time quantitative RT-PCR specific for the RdRp gene of SARS-CoV-2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Library-quality was assessed using Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Roche). Sequencing was performed on a NovaSeq S4 lane (Illumina ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1 μg of total RNA was reverse-transcribed with oligo dT primers using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed with removal of ribosomal RNA using the KAPA Stranded RNA-Seq kit with RiboErase (Roche). The RNA extraction and library preparation were performed by the same technician to minimize confounding effects ...
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bioRxiv - Microbiology 2020Quote: ... previously published assay (“one-step real-time RT-PCR E assay”) using the One-Step RT-qPCR kit (Roche Diagnostics). For each run ...
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bioRxiv - Microbiology 2019Quote: ... Sendai Virus (SeV) and Modified vaccinia Ankara (MVA)-gfp viral stocks were extracted using the High Pure Viral Nucleic Acid Kit (Roche). When indicated ...
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bioRxiv - Molecular Biology 2021Quote: Serum creatinine was determined using the creatininase/creatinase specific enzymatic method described by Bömer using a commercial kit (Alpha Laboratories Ltd. Eastleigh, UK) adapted for use on a Cobas Fara centrifugal analyser (Roche Diagnostics Ltd ...
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bioRxiv - Microbiology 2021Quote: Total nucleic acid was extracted from 253 resuspended vaginal swab samples using the MagNA Pure Compact Nucleic Acid Isolation Kit (Roche) [33] ...
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bioRxiv - Plant Biology 2021Quote: ... Purified DNA amplicons with flanking T7 and SP6 promoters were used for in vitro synthesis of digoxigenin (DIG)-UTP-labelled single-stranded RNA sense and anti-sense probes by using T7 and SP6 polymerases from a DIG RNA Labelling Kit (Roche). The non-complementary sense probe was used as negative control for each gene ...
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bioRxiv - Microbiology 2021Quote: ... Library preparation and sequencing data processing were performed according to Nilsen et al (2020) except the library was quantified with the KAPA library quantification kit (universal; Roche) in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated anew from stored clinical samples (naso- and/or oropharyngeal swabs in GLY-medium) using the MagNA Pure 96 DNA and Viral NA Small Volume Kit (Roche) and was assessed with a single replicate to obtain a first indication of clinical specificity and sensitivity ...
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bioRxiv - Microbiology 2020Quote: ... the DNA was subjected to pre-hybridization and hybridization using a DIG High Prime DNA labeling and detection starter kit II (Roche) according to the manufacturer’s instructions with a DIG-labeled probe of ∼100-200 bp annealing in the targeted gene (for primer sequences ...
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bioRxiv - Molecular Biology 2021Quote: ... DAB staining was performed using a Ventana Ultraview DAB detection kit in a Ventana BenchMark XT processor (Ventana Medical Systems, a division of Roche). In addition ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNAse was inactivated with 1 µl of Stop Solution at 65°C for 10 min and RT was performed using Transcriptor First Strand cDNA Synthesis Kit (Ref. 04 897 030 001, Roche) in a final volume of 20 µl ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed using 200-500 ng of total RNA in the Transcriptor First Strand cDNA synthesis kit (Roche) with 60 μM random hexamer and 6.25 μM oligo(dT)18 primer ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... rRNA-depleted RNA-seq libraries were prepared from 100-1,000ng RNA per sample using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, #KK8560). RNA fragmentation was performed at 94°C for 6 minutes and 10 PCR cycles were used during library amplification with TruSeq single-index adapters (Illumina ...
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bioRxiv - Genomics 2020Quote: ... cDNAs were prepared using Superscript II reverse transcriptase (Life Sciences) using hexamer random primers and qPCR was performed using the SybrGreen aMster kit (Roche) on a LightCycler 480 II ...
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bioRxiv - Cancer Biology 2020Quote: ... CAL33-c5 and CAL33-c11were seeded in 96-well plates and cell viability was determined with the colorimetric Cell Proliferation Kit II (XTT) (Roche) 24 ...