Labshake search
Citations for Roche :
3951 - 4000 of 5471 citations for Rat Malonyl Coenzyme A Decarboxylase MLYCD ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... RNA was re-extracted from original material using the High Pure RNA Isolation Kit (Roche Diagnostics GmbH Mannheim). A multi segment RT-PCR amplification was performed using the Superscript III high-fidelity RT-PCR Kit (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Libraries were quantified by qPCR using the Library Quantification Kit for Illumina sequencing platforms (KAPA Biosystems, cat#KK4824) using 7900HT Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... RNA from mouse brainstem or primary cell cultures was isolated using the High Pure RNA Isolation Kit (Roche). HiPSC-derived cells were lysed in RLT Plus buffer (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... 500 ng of RNA was prepared for sequencing using the KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems). Libraries were sequenced on the NextSeq 500 (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... quantitative PCR (RT-qPCR) with the SensiFAST SYBR No-ROX One-Step Kit in a LightCycler 96 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ul of each sample were used for library quantification using the KAPA Library Quantification Kit (Roche #KK4923). The rest of the DNA eluate (20ul ...
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bioRxiv - Plant Biology 2023Quote: ... Hybridization and probe detection was performed using a DIG Luminescent Detection Kit for Nucleic Acids (Roche Diagnostics, UK) as per the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: Low-pass WGS libraries were created using the Kapa Biosystems Hyper Prep kit (KAPA Biosystems, Capetown, South Africa) using 11.8 ng/ml of G4_1 ...
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bioRxiv - Cancer Biology 2023Quote: Proliferation was measured by using 5-bromo-2’-deoxy-uridine (BrdU) labelling and detection kit (Roche, BSL, CH) as described earlier (25) ...
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bioRxiv - Neuroscience 2023Quote: ... The assay was performed using the In Situ Cell Death Detection (TUNEL) Kit with TMR Red (Roche, 12156792910) as previously described13 ...
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bioRxiv - Microbiology 2023Quote: The RNA of all cell culture supernatants was extracted using High Pure Viral RNA kit (Roche, REF.11858882001). Viral RNA quantification in each sample was performed by quantitative reverse transcription PCR (RT-qPCR ...
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bioRxiv - Genomics 2023Quote: ... We quantified libraries by qPCR using the Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, cat#KK4824) and the 7900HT Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... The optimal numbers of PCR cycles were pre-determined using the KAPA Real-Time Library Amplification Kit (Roche) as described previously (Tanegashima et al ...
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bioRxiv - Immunology 2023Quote: ... 100 ng total RNA was reverse transcribed into cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Digoxigenin-labeled cRNA probes were prepared via in vitro transcription (DIG RNA labeling kit; Roche Diagnostics, Basel, Switzerland) from the Prokr2 template available from Genepaint (https://gp3.mpg.de/viewer/setInfo/MH1790/12) ...
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bioRxiv - Cancer Biology 2022Quote: ... or PPIA) was performed in duplicates with fast start universal SYBR Green master (ROX) PCR kit (Roche, 04913914001) using QuantStudio7 (Thermofisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... Both probes were labeled with fluorescein or digoxigenin (DIG) using RNA labeling kit (Roche Applied Science, Mannheim, Germany). The heads of fish at 4 mpf were fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Genetics 2023Quote: ... cDNA was synthesized with Anchored-oligo (dT)18 primers and the Transcriptor First Strand cDNA Synthesis Kit (Roche). cDNA was diluted five-fold with water and 2-4μl of the diluted cDNA served as template for each qRT-PCR as described previously 4 ...
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bioRxiv - Developmental Biology 2023Quote: ... The library pool was quantified by qPCR using a Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems). Paired-end 75bp sequencing was carried out by clustering 1.5pM of the library pool on a NextSeq 500 sequencer (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... Libraries preparation were performed with a Kapa Hyper Prep Kits with KAPA Library Amplification Primer Mix (Roche, 07962347001), quantified by Qubit (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 ng of DNA were used to prepare libraries using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR ...
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bioRxiv - Physiology 2023Quote: ... the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) in situ cell death detection kit (Roche, Basel, Switzerland) was used according to the manufacturer’s instructions (including incubation for 1 h at 37 °C) ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed with 1 μg of purified RNA using Transcriptor First Strand cDNA synthesis KIT (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Sequencing library preparation and probe capture enrichment were performed according to the SeqCap EZ Developer Kit (Roche NimbleGen) or the SeqCap RNA Developer Enrichment Kit (Roche NimbleGen) ...
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bioRxiv - Neuroscience 2023Quote: TUNEL staining was performed for paraffin sections using the In Situ Cell Death Detection Kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... LDH was measured using the Cytotoxicity Detection Kit according to the manufacturer’s instructions (ref: 11644793001, Roche, Basel, Switzerland) and absorbance (ABS ...
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bioRxiv - Genetics 2023Quote: ... mRNA-seq libraries were constructed from 600ng of total RNA using the KAPA mRNA HyperPrep Kit (Kapa Biosystems). Three independent biological replicates were analyzed for each genotype ...
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bioRxiv - Microbiology 2023Quote: ... Upon reaching OD600= 0.3 total RNA was extracted from cells using a High Pure RNA Isolation Kit (Roche). Samples at different time points were centrifuged for 5 min at 2,000 relative centrifugal force and pellets were flash-frozen in liquid nitrogen ...
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bioRxiv - Genomics 2023Quote: ... A digoxigenin labeled probe was generated by PCR following the manufacturer’s instructions (PCR DIG Probe Synthesis Kit,Roche). The 993 bp probe (Cd709 chr1 ...
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bioRxiv - Microbiology 2023Quote: ZIK1 sense and antisense RNA probes labeled with digoxigenin were synthesized using the DIG Northern Starter Kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA libraries for all samples were prepared using a KAPA Hyper Prep kit and PCR-free protocol (Roche). For each genotype ...
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bioRxiv - Cell Biology 2023Quote: ... and 100 / 30 ng (immunoprecipitation/input) used for library preparation using KAPA LTP library preparation kit (KAPA Biosystems). Library preparation was stopped prior to PCR amplification ...
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bioRxiv - Microbiology 2023Quote: ... hybridization capture was performed using the SHS method according to the SeqCap EZ Reagent Kit Plus (Roche NimbleGen) with modifications to the number of hybridization captures and the number of post-capture ligation-mediated PCR (LM-PCR ...
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bioRxiv - Plant Biology 2023Quote: ... transferred on nylon membrane and hybridized with DIG-labelled probes obtained by PCR DIG Probe Synthesis Kit (Roche) with specific primers (see table primers list) ...
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bioRxiv - Microbiology 2024Quote: ... Draining lymph nodes were harvested and preserved in a lysis solution from High Pure DNA Isolation Kit (Roche).
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bioRxiv - Developmental Biology 2024Quote: The RNA probes for detected genes were synthesized using a DIG RNA Labeling Kit (SP6/T7) (Roche, Germany). ISH was performed on 5 μm 4% paraformaldehyde-fixed paraffin-embedded sections from the testicular tissues ...
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bioRxiv - Cell Biology 2024Quote: AST and ALT levels were determined with a Kit (AST: Cat# 04467493190, Roche; ALT: Cat No 04467388190, Roche) according to International Federation of Clinical Chemistry and Laboratory Medicine (IFCC ...
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bioRxiv - Cell Biology 2024Quote: Libraries were constructed on 150 ng of input DNA with the KAPA HyperPlus kit (Roche Sequencing, CA, USA) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems, Wilmington, MA, USA). Pooled libraries were paired-end sequenced on a High Throughput Run Mode flow cell with the V4 sequencing chemistry on an Illumina HiSeq 2500 platform (Illumina ...
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bioRxiv - Cell Biology 2024Quote: AST and ALT levels were determined with a Kit (AST: Cat# 04467493190, Roche; ALT: Cat No 04467388190, Roche) according to International Federation of Clinical Chemistry and Laboratory Medicine (IFCC ...
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bioRxiv - Genomics 2024Quote: ... we constructed PE and SE libraries using the KAPA Hyper Library Preparation Kit (Kapa Biosystems, Woburn, MA, USA) and the Accel NGS 1S plus DNA kit (Swift BioSciences ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA (2 µl) was added to the mixture with FastStart SYBR Green Master Mix kit (Roche, Basel, Switzerland) and specific primers SP_F 5’-GAC-CAG-TCG-AAC-GCA-CAT-TG-3’ and SP_R 5’-CGG-AGA-GGG-TTG-TTG-TGT-CT-3’ ...
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bioRxiv - Molecular Biology 2024Quote: The peptidyl-tRNAs in the PTS buffer were isolated by using a High Pure miRNA Isolation Kit (Roche), as described previously (54) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared uniquely dual-indexed libraries for Illumina sequencing using the KAPA HyperPrep Kit (Roche, Indianapolis, Indiana, USA) using one-quarter reagent volumes ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted from mouse tail clippings and PCR amplified using the KAPA Mouse Genotyping Kit (Roche) following manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... The total RNAs were converted into individually barcoded polyadenylated mRNAseq libraries with the Kapa HyperPrep mRNA kit (Roche). Libraries were barcoded with Unique Dual Indexes (UDI’s ...
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bioRxiv - Systems Biology 2024Quote: Paraffin-embedded tissue sections were processed with an in-situ cell-death detection kit (11684795910; Roche, Basel, Switzerland) for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling ...
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bioRxiv - Molecular Biology 2020Quote: Cryosections (10μM) of ventricular tissue were fixed in 4% paraformaldehyde and stained with In-situ Cell Death Detection kit (Roche). Sections were imaged on a laser-scanning confocal microscope (LSM 510 ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labelled antisense and sense RNA probes were synthesized by in vitro transcription using DIG-labelled UTP according to the manufacturer’s instructions (DIG RNA labelling kit, Roche). Primers used were as follow ...
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bioRxiv - Genomics 2020Quote: ... all multiplexed single-cell libraries (n = 30) were quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche) and pooled in an equimolar ratio ...