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Citations for Roche :
351 - 400 of 3664 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 1 µl of the newly synthesized cDNA was then used to perform quantitative RT-PCR was in LightCycler 480 SYBR Green I Master (Roche, 04707516001) according to the protocol below on a LightCycler 480 Instrument or the QuantStudio 6 Flex instrument (Applied Biosystems ...
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bioRxiv - Physiology 2021Quote: ... DNase treatment was performed on 300 ng RNA prior to Reverse Transcription Polymerase Chain Reaction (RT-PCR) using RNAse-free DNAse I (Roche, # 04716728001) at 37°C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The same proportion of cDNA products of each sample was used as template for the quantitative RT-PCR reaction with Light Cycler 480 SYBR Green I Master Mix (Roche, 04707516001). The Ct value was used to represent the absolute amount of EU-RNAs in each sample ...
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bioRxiv - Microbiology 2022Quote: ... all samples underwent primary PCR amplification (30 cycles) using the conventional V4 primers (515F-806R) and KAPA HiFi Hot Start DNA polymerase (KAPA Biosystems, USA), and secondary PCR was performed to add dual-indexes (IDT ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μl of TruSeq PCR primer cocktail (50 μM) and 20 μl of KAPA HiFi HotStart ReadyMix (KAPA Biosystems, cat. no. KK2601). USER digestion was performed at 37 °C for 15 min ...
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bioRxiv - Genetics 2019Quote: ... of each library was used for bar-coding amplification using dual-barcoded single-stranded library adapters44 as primers in the following 100 μl volume reaction: 10 μl 10x PCR Buffer + MgCl2 (Roche, Basel, Switzerland) 0.4 μM of each primer ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of TruSeq PCR primer cocktail (50 μM) and 20 μl of KAPA HiFi HotStart ReadyMix (KAPA Biosystems, cat. no. KK2601). USER digestion was performed at 37 °C for 15 min ...
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bioRxiv - Cancer Biology 2019Quote: ... The primers used for real-time PCR were designed based on the Universal Probe Library (Roche, Roche Life Science, Pleasanton, CA, USA) with GAPDH as reference ...
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bioRxiv - Plant Biology 2020Quote: ... Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG; and Rev 5’-GTGGCTGAGGCTTCTTTTTG) and the PCR DIG Probe Synthesis Kit (Roche Diagnostics, Switzerland). The signals were detected by CDP-Star (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2020Quote: ... to amplify the ligation product with 5 PCR cycles using 2x KAPA-HiFi HS Ready Mix and 10X KAPA primer mix (Roche Kapa Biosystems). The libraries were sequenced on HiSeq 4000 or NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... was used for PCR amplification of all tested genome fragments from AF associated loci (for primers used see Supplementary Table S1) using Expand High Fidelity PCR system (Roche ref. 11732650001). Primers were design using NEBuilder assembly tool to have 20-basepair (bp ...
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bioRxiv - Microbiology 2023Quote: The qRT-PCR experiments were performed using specific oligonucleotide primers and hot-start polymerase (SYBR Green Fast Master Mix; Roche Diagnostics, Germany). The amplification cycles were performed using a C1000 Touch Thermal cycler (Biorad ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA levels were determined using gene-specific primers by SYBR green nucleic acid labeling (Selleckchem PCR Mastermix, #B21202) in a Lightcycler 480 (Roche, Indianapolis, IN). Fold change was calculated using the delta delta C(t ...
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bioRxiv - Plant Biology 2020Quote: ... MpC1HDZ was amplified from cDNA using a specific forward primer and polyT reverse primer (Roche) and ligated into pGEMT ...
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bioRxiv - Genetics 2022Quote: ... and DIG Wash and Block Buffer Set (Roche 11585762001) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... KAPA single indexed adaptor set A (KAPA biosystems, KK8701) was used for adaptor ligation ...
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bioRxiv - Immunology 2022Quote: ... measured by RT ELISA (Roche), per well (MOI 0.2 ...
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bioRxiv - Cell Biology 2020Quote: ... Mip1α and housekeeping gene β-actin was evaluated by quantitative RT-PCR using the LightCycler 480 SYBR Green 1 Master kit and LightCycler 480 II (Roche, Indianapolis, IN) and oligos ...
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bioRxiv - Molecular Biology 2021Quote: RNA template (500 ng) was used for one-step RT-PCR amplification protocol (SYBR Green I RNA amplification kit, Roche, Basel, Switzerland). Primers were designed using the LC Primer/Probe design software (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were subsequently transferred to 384-well plates for analysis by RT-qPCR on the 480 Real-Time PCR system (Roche, Basel, Switzerland) using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was added to optimized 10 µM primer/probe mix in Mastermix (Quantabio, qScript™ XLT One-Step RT-qPCR ToughMix®) and run on StepOne Plus real-time PCR (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression levels were quantified by Reverse Transcription quantitative polymerase chain reaction (RT–qPCR) using the LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland). Cycling parameters were set at (i ...
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bioRxiv - Microbiology 2021Quote: ... and Fwd-P2 (5′-CCATCTCATCCCTGCGTGTCTCCGACTCAGBBBBBBAGARTTTGATCYTGGTTCAG and the reverse primer was Rev1B (5′-CCTATCCCCTGTGTGCCTTGGCAGTCTCAG. The PCR products were sequenced on the 454 GS FLX Titanium sequencer (Hoffmann-La Roche, LTD; Basel, Switzerland). Through the Research Alliance for Microbiome Science Registry (IRB no ...
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bioRxiv - Plant Biology 2023Quote: ... The screening for hCas9 presence was performed using primers reported in Data S1 by PCR using KAPA HIFI Taq (Kapa Biosystems, Boston, USA) with the following program ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and primer premix (Kapa Biosystems, UK). Libraries were pooled in equimolar concentrations and pairs of 125 bp sequences were produced on two lanes of Illumina HiSeq 2500 at Biomedical Research Centre Genomics ...
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bioRxiv - Physiology 2019Quote: ... and random hexamer primers (Roche Diagnostics). Quantitative real-time PCR was performed with primers for SMAD3 ...
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All-Flesh Tomato Regulated by Reduced Dosage of AFF Provides New Insights into Berry Fruit EvolutionbioRxiv - Plant Biology 2021Quote: ... The primers were designed by Roche LCPDS2 software and were synthesized by Beijing TsingKe Biological Technology Co. ...
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bioRxiv - Neuroscience 2021Quote: ... random hexamer primers (50 µM; Roche), RNasin (20 U ...
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bioRxiv - Physiology 2020Quote: ... with anchored oligo-dT primers (Roche). qPCR was run in a 384-well plate format on a ViiA 7 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... KAPA Unique Dual Indexed primers (Roche) were incorporated by PCR (4 cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... miR-122 inhibition was carried out by transfection with AntagomiR 122 (Miravirsen, SPC-3649; Roche, formerly Santaris Pharma). For Smad4 silencing in mouse primary hepatocytes ...
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bioRxiv - Genetics 2021Quote: ... The RT-PCR reaction was carried out in a Light Cycler 480 fluorescence quantitative meter (Fritz Hoffmann-La Roche Co. Ltd., Basel, Switzerland).
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bioRxiv - Plant Biology 2019Quote: ... were used as template for quantitative real time RT-PCRs which were performed in 384-well plates using a LightCycler 480 II detection system (Roche Diagnostics, Mannheim, Germany). GoTaq PCR Mastermix (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Genotyping was conducted by real-time polymerase chain reaction (RT-PCR) and subsequent high resolution high-resolution melting on a Cobas Z480 Light Cycler (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time RT-PCR was then performed using the KAPA SYBR FAST qPCR Master Mix (2x) Kit (Kapa Biosystems, Cape Town, South Africa) in LightCycler 480® (Roche ...
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bioRxiv - Genomics 2020Quote: ... and the DIG Wash and Block Buffer Set (Roche 11585762001), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and the DIG Wash and Block Buffer Set (Roche 11585762001). 5 to 10 ug of genomic DNA (roughly genomic DNA extracted from about 15 adult flies ...
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bioRxiv - Neuroscience 2020Quote: ... 0.2 μM of both reverse and forward primers and the PCRs were run on a Roche Lightcycler 480 thermocycler (Roche Applied Science, Basel, Switzerland). Each sample and primer pair was run in triplicates ...
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bioRxiv - Genetics 2022Quote: ... and amplifying the exon regions of targeted genes by using the primers with SP6 sequence (ATTTAGGTGACACTATAGAAGNG) according to the product description of KAPA HiFi PCR Kit (Roche Diagnostics, cat. no. 07958927001). Sense and antisense digoxigenin (DIG)-labeled probes were synthesized from the purified PCR product using DIG RNA Labeling Kit (Roche ...
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bioRxiv - Immunology 2022Quote: ... with oligo-dT primers and then amplified them by 22 cycles of PCR using the KAPA HiFi Hot Start Ready Mix (Kapa Biosystems, Wilmington, MA, USA) with 5Me-isodC-TSO and ISPCR primers ...
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bioRxiv - Immunology 2019Quote: ... MicroRNA RT-qPCR was performed with ExiLENT SYBR® Green master mix (Exiqon) using LightCycler ® 96 Real-Time PCR System (Roche Life Science). Primers used in this study are ...
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bioRxiv - Immunology 2019Quote: ... RT-PCR was performed on the LightCycler® 480 instrument using the LightCycler® 480 SYBR Green I Master mix (Roche Diagnostics, Mannheim, Germany) and oligonucleotides listed in Table 1 ...
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bioRxiv - Genomics 2021Quote: ... Quantification of final library before loading on MiSeq sequencer was performed using the Kapa quantification kit (RT-PCR) for next generation sequencing with the Illumina platform (Kapa Biosystems, Wilmington, MA USA). Sequence reads were quality filtered and assembled using CLC Genomics Workbench 8.0.1 (CLCbio ...
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bioRxiv - Cancer Biology 2021Quote: ... First-strand cDNA synthesis and RT-qPCR was carried out using One step RT QPCR reagent (Roche). Resulting signal was detected on Applied Biosystems Real Time PCR System ...
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bioRxiv - Neuroscience 2019Quote: ... with random hexamer primers (Roche Diagnostics, Germany) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... using random primers p(dN)6 (Roche). Quantitative PCR reactions were performed in a BioRad CFX96 system employing the SYBRgreen fluorescent reagent (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... All probes and primers were from Roche or Thermofisher.
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bioRxiv - Neuroscience 2020Quote: ... using random primers p(dN)6 (Roche). Quantitative PCR reactions were performed using standard protocols using EvaGreenTM in the Stratagene Mx3000P system (Agilent Technologies) ...
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bioRxiv - Pathology 2022Quote: ... All probes and primers were from Roche. Primer sequences:
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bioRxiv - Developmental Biology 2023Quote: ... and 500 ng of random primer (Roche) at 25°C for 15 minutes ...