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Citations for Roche :
351 - 400 of 1209 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... skin tissues were homogenized at a 10% (w/v) concentration in a lysis buffer containing 2 mM CaCl2 and 0.25% (w/v) collagenase A (Roche) in Tris-Buffered Saline (TBS) ...
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bioRxiv - Microbiology 2024Quote: ... 0.1% (v/v) NP-40 and 0.5 mM Tris carboxy ethyl phosphene (TCEP) buffer supplemented with protease inhibitor cocktail (Roche), flash-frozen in liquid nitrogen and lysed under cryogenic conditions using a Retsch MM400 (Retsch) ...
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bioRxiv - Neuroscience 2020Quote: RNA was purified from ∼50 mg of homogenized tissue from all organs by using the MagNAPure 96 system and the MagNA Pure 96 DNA and Viral NA Large Volume Kit (Roche) following the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... This was run on a LightCycler 96 (Roche) using the following program ...
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bioRxiv - Biochemistry 2020Quote: ... PCR was performed on LightCycler 96 Instrument (Roche) as described previously [36].
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bioRxiv - Microbiology 2019Quote: ... with a LightCycler 96 instrument (Roche Applied Science), for a total volume of 20µl of reaction mixture ...
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bioRxiv - Neuroscience 2021Quote: ... The reaction was performed using LightCycler 96 (Roche) with the following conditions ...
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bioRxiv - Microbiology 2022Quote: ... with the LightCycler® 96 system (Roche, USA). Serial dilutions of the DEMV-2 plasmid with known concentrations were used to establish the standard curve and evaluate the amplification efficiency of the system ...
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bioRxiv - Cell Biology 2022Quote: ... on a Roche Lightcycler 96 (Roche Life Science). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2021Quote: ... the MagNa Pure 96 System (Roche, Penzberg, Germany) was used according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... qPCR was performed on a LightCycler 96 (Roche). Relative quantification was carried out by using the 2-ΔCt method ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were run on Lightcycler 96 system (Roche) with the following three-step cycling conditions ...
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bioRxiv - Biochemistry 2021Quote: ... qPCR was performed on a LightCycler 96 (Roche) with the following settings ...
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bioRxiv - Plant Biology 2020Quote: ... with Light Cycler 96 (Roche Life Science, CA). For normalization ...
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bioRxiv - Microbiology 2021Quote: ... on a LightCycler 96 real-time thermocycler (Roche) following a published method [52 ...
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bioRxiv - Cancer Biology 2022Quote: ... and specific primers on the LightCycler 96 (Roche). Relative target gene expression was determined by comparing average threshold cycles (CT ...
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bioRxiv - Microbiology 2022Quote: ... Experiments were performed on a LightCycler 96 (Roche) using the SensiFAST Probe No-ROX kit (Bioline) ...
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bioRxiv - Plant Biology 2022Quote: ... and the LightCycler 96 system (Roche, Basel, Switzerland). Details regarding the quantitative real-time PCR primers are provided in Supplemental Table S2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and loaded into a LightCycler 96 Instrument (Roche). Pre-incubation at 95°C for 90 sec followed by 40 cycles of 95°C 10 sec ...
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bioRxiv - Microbiology 2022Quote: ... on the MagNA Pure 96 Instrument (06541089001, Roche). Median neutralisation breakpoints (CPE ...
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bioRxiv - Microbiology 2023Quote: ... under thermocycler PCR system (LightCyler 96, Roche, Germany). The PCR program was as follows ...
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bioRxiv - Microbiology 2023Quote: ... using white LightCycler480 Multiwell Plate 96 plates (Roche). One 20 µl reaction contained 250 nM of hydrolysis probe ...
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bioRxiv - Immunology 2024Quote: ... and run on LightCycler 96 Systems (Roche Holding). Transcript levels of STAT1 ...
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bioRxiv - Plant Biology 2024Quote: ... using a LightCycler 96 System (Roche, Basel, Switzerland). The transcript level of each gene was normalized to that of PP2A (At1G13320) ...
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bioRxiv - Cancer Biology 2023Quote: ... with a LightCycler 96 System (Roche, Basel, Switzerland). Relative expression levels of target genes were calculated utilizing the 2-ΔΔCt method (Ct ...
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bioRxiv - Cancer Biology 2024Quote: ... and specific primers on the LightCycler 96 (Roche). Relative target gene expression was determined by comparing average threshold cycles (CT ...
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol and 1 mM Tris(2-carboxy-ethyl) phosphine (TCEP) containing an EDTA-free protease inhibitor tablet (Roche). The cell suspension was sonicated on ice and clarified by centrifugation at 27,000g for 15 min ...
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bioRxiv - Cell Biology 2022Quote: All controls contained 4% (v/v) 25x concentrated protease inhibitor cocktail solution (cOmplete Protease inhibitor cocktail tablets, Roche, Mannheim, Germany) in accordance with the PPP samples.
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bioRxiv - Cell Biology 2022Quote: Cells were harvested in cold PBS and subsequently lysed in 1% (v/v) Triton X-100 in PBS supplemented with Protease Inhibitor Cocktail (Roche), N-ethylmaleimide and sodium orthovanadate ...
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bioRxiv - Biophysics 2019Quote: ... 10 mM Na4P2O7 and 10 mM EDTA supplemented with 1% (v/v) Triton X-100 and one complete protease inhibitor cocktail tablet (Roche)) ...
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bioRxiv - Plant Biology 2019Quote: ... 60 mM KOAc, 10 mM MgOAc, 0,5% [v/v] Nonidet P-40 and proteinase inhibitor cocktail [cOmplete™, COEDTAF-RO, Roche]). After a solubilization step (15 min ...
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bioRxiv - Microbiology 2019Quote: Whole cell lysates were prepared by suspending purified parasite pellets in whole cell lysis buffer (1XPBS, 1% v/v Triton X-100) containing Protease Inhibitors (cOmplete EDTA-free, Roche). 24 h in vitro ookinetes were also subjected to cellular fractionation using the following method ...
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bioRxiv - Genetics 2021Quote: Animals were resuspended in lysis buffer (50 mM Tris/HCl pH 7.4, 100 mM NaCl, 1% v/v SDS, complete protease inhibitor cocktail (Roche Diagnostics)) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM TCEP 10 % v/v glycerol) supplemented with 4x EDTA-free protease inhibitor cocktail tablets (Roche, cat. No. 11836153001) and 4 μg/ml DNase I ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and 5% (v/v) glycerol and Supplementarylemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... cells were gently washed twice with 500 μl cold PBS and directly lysed in 50 μl of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... N’-tetraacetic acid (EGTA) and 5% (v/v) glycerol and supplemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Genetics 2022Quote: ... COS-7 cells transiently expressing POPDC constructs with the appropriate epitope tag were lysed using a 1% (v/v) Triton X-100 based lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche). Lysates were subjected to Co-IP using the ProFoundTM c-Myc Tag IP/co-IP Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 mM imidazole and 10 % (v/v) glycerol] supplemented with 1 mM PMSF and complete EDTA-free protease inhibitor cocktail (Roche). After centrifugation at 45 000 x g supernatant was filtered (0.45 µm ...
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bioRxiv - Molecular Biology 2022Quote: ... re-suspended in buffer A (50 mM HEPES/NaOH pH8, 500 mM NaCl, 10% (v/v) glycerol) supplemented with protease inhibitors (Roche, complete mini ...
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bioRxiv - Molecular Biology 2023Quote: Thawed and washed cell pellets were lysed in 1 mL sterile PBS with 10% (v/v) glycerol and 1x cOmplete mini protease inhibitor cocktail (Roche). Ten micrograms of protein were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed 10 min on ice in 1% (v/v) Triton X-100 in TBS supplemented with protease inhibitor (cOmplete, Roche) and phosphatase inhibitor (PhosStop ...
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bioRxiv - Biochemistry 2022Quote: ... 0.05% (v/v) Tween-20) supplemented with 10 μg/mL DNase I and 1 cOmplete protease inhibitor cocktail tablet (Roche) per L buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was incubated with 5% v/v pre-immune rabbit sera and 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody incubation was performed for 16 h at 4°C in AB buffer supplemented with 1% v/v Fetal Calf Serum and anti-DIG antibody coupled to alkaline phosphatase (1∶5000 dilution; Roche). Sections were then washed thoroughly in AB and equilibrated in alkaline phosphatase buffer (AP - 0.1 M Tris–HCl pH ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001]). 40 µg of protein was loaded onto NuPAGE-Novex Bis-Tris 4%–12% gradient gels (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed in sterile-filtered PBS (pH 7.2) and resuspended in sterile-filtered ice-cold FACS buffer (2% (w/v) bovine serum albumin fraction V (Roche, Switzerland) and 0.01% sodium azide (w/v ...
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bioRxiv - Cell Biology 2020Quote: Cells were transfected with 250-800 ng DNA per well of a 12-well plate or 500 ng per well of a 6-well plate using X-tremeGENE 9 DNA transfection reagent (Roche), and harvested after 24 h.
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bioRxiv - Cell Biology 2022Quote: ... incubated with 0.2% (w/v) collagenase II (Roche) in DMEM containing 20% FCS and antibiotic-antimycotic (100× ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 % (w/v) hyridization blocking reagent (Roche, 339450), 0.3 % (v/v ...