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Citations for Roche :
351 - 400 of 698 citations for Recombinant Yellow Fluorescent Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... washed and dried before digestion of proteins with 12.5 ng/μl of trypsin (Roche) in 25 mM NH4CO2 and 10 mM CaCl2 overnight at 25°C ...
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in 1% SDS containing PBS supplemented with protein inhibitor cocktail (Roche) and 250 U/ml bensonase (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested using Endoproteinase Lys-C at 1:100 w/w (Roche, 11058533103) at 37 °C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... The protein lysates were extracted in presence of a protease cocktail tablet (Roche Diagnostics) using Cell Lysis buffer (ThermoFisher) ...
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bioRxiv - Physiology 2020Quote: ... Proteins were extracted using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche). Samples were vortexed for 5 seconds ...
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bioRxiv - Biochemistry 2020Quote: ... MR-Fc was purified from cell culture supernatant using Protein A-agarose beads (Roche). For ELISA experiments ...
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bioRxiv - Microbiology 2020Quote: ... Antibody was removed by the addition of 100 µl homogeneous Protein G-Agarose (Roche) and a further incubation for 3 h ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected with a mouse anti-GFP antibody (1:5000; Roche). Immunoblot results were quantified using Image J software (v1.8.0).
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bioRxiv - Developmental Biology 2022Quote: ... The lysate was precleared by incubation with 50 μL protein-A agarose beads (Roche) for 1 h and then incubated with anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were generated using RIPA lysis buffer supplemented with protein inhibitor (Roche, #4693159001) and protein concentration was determined using Pierce™ BCA protein assay ...
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bioRxiv - Cancer Biology 2020Quote: ... Separated proteins were then transferred to a methanol-activated polyvinylidene difluoride membrane (PVDF, Roche) using wet transfer Mini-PROTEAN II electrophoresis system (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: ... GFP-tagged proteins were detected with a primary mouse antibody IgG1K Anti-GFP (Roche) diluted to 1:1000 in 5 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were then treated with 8 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 3 hours followed by 19 μg/sample of trypsin (Roche Cat ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were then extracted in an IP buffer with a phosphatase inhibitor (Roche, PhosSTOP), centrifuged at 1700g for 5 min and filtered through miracloth ...
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bioRxiv - Cancer Biology 2019Quote: Protein was extracted from cultured cells in RIPA buffer containing a protease inhibitor (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2019Quote: Total protein was collected in radioimmunoprecipitation buffer (RIPA) with PhosSTOP (Roche Diagnostics, Mannheim, Germany) and Complete Mini protease inhibitor cocktail tablets (EMD Chemicals Inc ...
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bioRxiv - Molecular Biology 2019Quote: ... Following a 30 min preclear using blocked Protein A or G agarose beads (Roche), the chromatin was incubated with 5 µg antibody overnight at 4 °C (antibodies are listed in Table S6) ...
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bioRxiv - Cell Biology 2019Quote: ... Treated cells were lysed in protein lysis buffer with protease and phosphatase inhibitors (Roche). Selected cytokine targets were measured in cell culture supernatants and cell lysates with commercially available Luminex® Multiplex Assays (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... released DNA was purified from protein components (High pure PCR Product Purification Kit, Roche) and chromatin quality analysed using EtBr agarose gel electrophoresis (1.3 % Biozym ME Agarose ...
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bioRxiv - Biochemistry 2020Quote: ... released DNA was purified from protein components (High pure PCR Product Purification Kit, Roche) and analysed using ethidium bromide (EtBr ...
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bioRxiv - Biochemistry 2020Quote: ... 10% (v/v) glycerol (TS130G) in the presence of cOmplete protein inhibitor cocktail (Roche). The resulting samples were clarified by centrifugation in an SS34 rotor (27,000 xg ...
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bioRxiv - Cell Biology 2020Quote: ... followed by addition of 5µl (packed volume) of protein G-coupled Agarose beads (ROCHE) and continued overnight incubation at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Protein was detected with 1:2,000 horseradish peroxidase (HRP)-conjugated anti-HA antibody (Roche).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested by adding 1 mL of Proteinase K (20 mg/mL) (Roche) for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... the supernatant was cleared with 50µl of Protein agarose G (Roche cat no. 11719416001) for 3 hours at 4 °C and subsequently incubated with 50 µl of Protein agarose G with corresponding primary antibody overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Phosphorylated proteins were visualized by chemiluminescence using Lumi-Light Western Blotting substrate (Roche, 12015196001) or SuperSignal West Femto Maximum Sensitivity substrate (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation (IP) of HA-tagged proteins was performed using Anti-HA Affinity matrix (Roche) under denaturing conditions ...
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bioRxiv - Cell Biology 2023Quote: ... blocked in 5% nonfat dry milk and proteins detected using monoclonal anti-GFP (Roche), monoclonal anti-PGK (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... His6-GFP and His6-mCherry proteins were purified with cOmplete His tag resin (Roche) according to the manufacturers protocols as previously described9 ...
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bioRxiv - Immunology 2023Quote: ... Pre-digested proteins and peptides were reduced with 10 mM dithiothreitol (DTT) (Roche, 10197777001) for 30 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... and protein was extracted using SDS lysis buffer93 with cOmplete protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5% (v/v) Triton X-100) containing 1X protein and phosSTOP inhibitor cocktails (Roche) for 5 min on ice and cleared by centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation with 20 μL of resuspended volume of Protein G beads (Roche) for 2 h at 4 °C to pull down bound proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was detected with 1:3,000 horseradish peroxidase-conjugated anti-HA (Roche, cat#12013819001) or anti-GFP (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Secreted IgGs were purified from cell supernatants with Protein A resin (Roche, Basel, Switzerland). IgGs were eluted at low pH using Protein A elution buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and organoids were resuspended in RIPA protein lysis buffer containing protease inhibitor cocktail (Roche) at day 7 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation of GFP/YFP-tagged proteins was performed with anti-GFP antibodies (11814460001, Roche) using a method we previously described (Ishii and Akiyoshi ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with rat α-HA (3F10, Roche), rabbit α-Myc (Cell Signaling) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated using SDS-PAGE and detected using a monoclonal antibody against GFP (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2021Quote: ... protein lysates were prepared in RIPA buffer supplemented with complete protease inhibitor (Roche Applied Science). Proteins were then separated on 6%–15% SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: Total proteins of cells were extracted using RIPA lysis buffer (Beyotime) with Protease Inhibitor (Roche) and Phosphatase Inhibitor (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins on the beads were digested with 0.1 μg/μl sequencing grade trypsin (Roche) overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were captured with 50 μl of 50% (w/v) protein-A agarose beads (Roche) for 3 hr at 4°C on a rotary shaker ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins were then subsequently digested at 37°C with Lys-C (Roche Applied Science) during 4 h then with trypsin (Sequencing Grade Modified ...
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bioRxiv - Biochemistry 2019Quote: ... then protein was eluted with 100 µM imidazole in the presence of protease inhibitors (Roche) and desalted using a PD-10 column ...
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bioRxiv - Molecular Biology 2019Quote: ... 300 ng protein was subjected to 10% SDS gel and electrotransferred onto PVDF membrane (Roche). After blocking for 1h with 3% BSA in PBS ...
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bioRxiv - Biochemistry 2019Quote: MBP Protein preparation was performed by a crushed Complete EDTA-free protease inhibitor tablet (Roche) was added to the cell pellet before being resuspended in lysis buffer (50mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... Resolved proteins were then transferred from polyacrylamide gels to 0.45 μm PVDF membranes (Immobilon, Roche) at 200 V for 1 hr ...
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bioRxiv - Immunology 2019Quote: Total protein was isolated by homogenizing mouse feces in PBS with protease inhibitor cocktail (Roche). Samples were rotated at 4°C for 4 hours and centrifuged at 16,000g for 15 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then digested by addition of 2 μL 20 mg/mL Proteinase K (Roche) and incubation for 2 h at 55° C ...