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Citations for Roche :
351 - 400 of 535 citations for M CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were resuspended in microsome buffer (50 mM MOPS, 0.5 M sorbitol, 5 mM EGTA, complete protease inhibitor (Roche)) and repelleted by centrifugation at 100□000 g for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... B-R18 labelled A1 EVs were resuspended in PBS buffer (0.2 M NaCl) containing a protease inhibitory cocktail (Complete Protease Inhibitory Tablets, Roche) and were stored at −20°C.
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bioRxiv - Physiology 2023Quote: ... approximately 15 mg of pulverized mouse intestinal tissue was solubilized in ice-cold urea Lysis buffer (8 M urea in 50 mM Tris, pH 8.0, 40 mM NaCl, 2 mM MgCl2, 1x Roche cOmplete ULTRA EDTA-free protease inhibitor mini tablet ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in 1 ml SEH buffer (20 mM HEPES pH 7.4, 1 mM EDTA, 0.6 M Sorbitol, 1x cOmplete™ Tablets mini EDTA-free protease inhibitor [Roche]). Resuspended yeast cells were transferred into screw cap tubes containing 500 µl glass beads ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell pellets were lysed with 200 μl buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were resuspended in buffer M (50 mM NaH2PO4, 50 mM Na2HPO4, pH 6.8) supplemented with 1 mg/ml lysozyme and protease inhibitor (Roche) and opened by sonication ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated for 1 h at room temperature (20– 25 °C) in 0.1 M PBS with 1% blocking reagent (Roche) and 0.3% Tween 20 (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2024Quote: ... Cell pellets were resuspended in lysis buffer (0.5 M NaCl, 20 mM Na3PO4 pH 7.4 and cOmplete™ Protease Inhibitor Cocktail (Roche) and then lysed using sonication ...
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bioRxiv - Cell Biology 2024Quote: Samples were lysed in buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from human samples using TriPure Isolation Reagent (Roche). RNA integrity (RIN ...
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bioRxiv - Genomics 2022Quote: ... the regions of interest were PCR amplified from human genomic DNA (Roche) using Taq polymerase (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: Human TGs were measured using the Cobas c 501 analyzer (Roche Diagnostic). For this purpose ...
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bioRxiv - Genetics 2023Quote: Candidate regions were PCR-amplified using human genomic DNA (Roche, Basel, Switzerland) as template and cloned in pGL4.23 Firefly luciferase reporter vector (Promega ...
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bioRxiv - Immunology 2023Quote: ... 50μM beta-mercaptoethanol and 10 ng/ml recombinant human IL-2 (Roche). Both human and murine CD4+ T cells were cultured for 46 hrs under humidified conditions at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... eosin and antibodies for human keratins AE1/AE3 (760-2135; Roche diagnostics). Metastasis quantification was reached through a review of 17 digitalized slides ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 1× Roche protease inhibitor cocktail) and incubated on ice for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: Frozen extracted mitochondria were lysed by sonication in 300 μl of lysis buffer (8 M urea, 50 mM ammonium bicarbonate, 1X protease inhibitors (Complete mini EDTA-free mixture [Roche Applied Science] ...
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bioRxiv - Molecular Biology 2020Quote: ... 100,000 cells were washed twice (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM spermidine, 1x Roche complete protease inhibitors) and attached to activated ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear pellets were lysed by sonication on ice in 10 mL lysis buffer (50 mM Tris pH 7.4, 0.5 M NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, 1x Roche complete protease inhibitor). After sonication ...
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bioRxiv - Molecular Biology 2021Quote: ... and then resuspended in 200 μL of SCE buffer (1 M sorbitol, 10 mM EDTA, 10 mM DTT, 100mM sodium citrate, 1 Roche mini-EDTA-free protease inhibitor tablet per 50 mL ...
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bioRxiv - Molecular Biology 2019Quote: ... Solidified plugs were transferred in 14 ml tubes with round bottom and incubate with 400 µl/plug of PK buffer (EDTA 0.125 M [pH 9.5], Sarkosyl 1%, PK 1 mg/ml (Roche, ref# 03115801001) and incubated 1-2 hours at 37°C with gentle agitation ...
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bioRxiv - Genomics 2019Quote: ... The supernatants from the digestion and initial decalcification step were purified using a 5 M guanidine-hydrochloride binding buffer with a High Pure Viral Nucleic Acid Large Volume kit (Roche). The extract was eluted in 100 μl of a 10mM tris-hydrochloride ...
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bioRxiv - Genomics 2020Quote: ... before incubation at 37°C with 1 µg of double stranded DNA (atr amplicon, Jones et al., 2003) in the presence of 1x M buffer (Roche). Filtrated supernatants (10 µl ...
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bioRxiv - Biochemistry 2020Quote: ... The thorax and the abdomen were then washed with 3 ml of pre-chilled 0.1 M KBP pH 7.4 homogenization buffer (HB) containing 1x protease inhibitor (Roche Complete ULTR). The complex was homogenized in 20 ml of HB using 40 ml glass Dounce homogenizer with a loose B pestle (Wheaton Science ...
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bioRxiv - Neuroscience 2021Quote: ... were dissociated onto poly-d-lysine (50 ug/mL diluted in 0.1 M Borate Buffer, pH 8.6) and laminin (10 ug/mL; Roche, Basel, Switzerland) coated 6 well plastic plate ...
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bioRxiv - Cell Biology 2021Quote: ... air-dried and resuspended in 50 µl of 8 M urea in 50 mM triethylammoniumbicarbonate including protease inhibitor cocktail (Roche). Afterwards proteins were reduced with 5 mM dithiothreitol for 1h at 25°C and alkylated with 40 mM chloroacetamide for 30 min in the dark ...
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bioRxiv - Biochemistry 2021Quote: Cells were washed 2x with PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1X PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Cell Biology 2021Quote: ... 150 mM KOAc, 2 mM MgCl2, 1 mM CaCl2, 0.2 M sorbitol, 10mM PMSF, 2x protease inhibitor cocktails [Roche, 11873580001]), dropped into liquid nitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
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bioRxiv - Cancer Biology 2020Quote: Protein extracts were prepared from drug-treated cells by lysis on ice for 20 minutes in M-PER Mammalian Protein Extraction Reagent (ThermoScientific) supplemented with protease and phosphatase inhibitors (Roche). Lysates were centrifuged at 16,000 RPM for 20 minutes at 4°C and the supernatants were assessed for concentration via a Bradford assay ...
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bioRxiv - Pathology 2022Quote: ... and brain tissues were homogenized using a Teflon-glass homogenizer in ice-cold homogenization buffer (0.32 M sucrose, 5 mM EDTA, 10 mM Tris-HCl, and pH 7.4, phosphatase inhibitor cocktail tablet; Roche, Mannheim, Germany), centrifuged at 4500 rpm for 10 min at 4°C and the supernatants were collected.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... hippocampi from each animal were homogenized in ice-cold Homogenization buffer (2 M sucrose, 500 mM HEPES (pH 7,4)) containing complete Protease Inhibitor (Roche/Sigma) and spun down for 10 min at 1000 x g at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μm sections were stained with H&E and Masson trichrome (Trichrome II Blue staining kit at Nexus special stainer; Roche). Immunohistochemical analysis was performed on cryosections and paraffin sections using a Bench Mark XT automatic staining platform (Ventana ...
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bioRxiv - Biochemistry 2022Quote: ... 250,000 cells (per antibody/cell line combination) were washed twice (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM spermidine, 1x Roche complete protease inhibitors) and attached to ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Developmental Biology 2019Quote: ... The frozen cells and organoids were crushed by using Multi-beads shocker (Yasui Kikai, Japan) and subsequently lysed by sonication in 9.8 M urea with protease inhibitor cocktail (cOmplete™; Roche) and phosphatase inhibitor cocktail (PhosSTOP™ ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were washed three times with PBS and harvested directly in 8 M Urea lysis buffer supplemented with phosphatase inhibitor (PhosSTOP, Roche) and protease inhibitor (cOmplete mini EDTA-free ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were pelleted at 1350xg for 5 min at 4°C and lysed in 5 mL LB2 (10 mM Tris-Cl pH 8.0, 5 M, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1 mM PMSF, Roche protease inhibitors) for 10 min at RT with rotation ...
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bioRxiv - Microbiology 2020Quote: Western blot analysis of infected and uninfected erythrocytic material performed on protein extracts prepared by resuspending cells in M-PER mammalian protein extraction reagent (Pierce) supplemented with 1x protease inhibitory cocktail (EDTA free) (Roche), 300 mM benzamidine ...
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bioRxiv - Neuroscience 2020Quote: Mouse brains were homogenized with lysis buffer (50 mM Tris pH 7.6, 0.15 M NaCl and cOmplete protease inhibitor cocktail (Roche Diagnostics 11697498001)) using a Multi-bead shocker MB (Yasui-Kikai) ...
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bioRxiv - Cancer Biology 2022Quote: ... staining experiments were performed on 4µm thick formalin fixed and paraffin embedded (FFPE) tissue sections using a Ventana automated tissue staining system (BenchMark Ultra, Roche). For IHC ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed by the lysis buffer 10 M urea containing the cOmplete™ protease inhibitor cocktail (Roche, #11697498001). The cell tubes were ultrasonicated at 4 ℃ for two cycle (1 min per cycle ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with ice-cold PBS and scraped into lysis buffer (1 mM EPPS, 8 M urea, protease (complete mini, EDTA-free) inhibitors (Roche) and PhosSTOP phosphatase inhibitor (Roche)) ...
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bioRxiv - Neuroscience 2023Quote: ... Five P2 mouse brains were homogenized in 5 mL of homogenization buffer (5 mM Tris-HCl, 0.32 M sucrose, 1 mM MgCl2) supplemented with EDTA-free protease inhibitors (cOmplete™, Roche), then centrifuged at 1,660 x g for 15 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μL 1 M spermidine, 500 µL 10% Triton X-100, and 10 ml glycerol in 38 ml dH2O, 1 Roche Complete Protease Inhibitor EDTA-Free (Millipore Sigma 11873580001)) ...
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bioRxiv - Cell Biology 2023Quote: Cells were washed 2x with 1x PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1x PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Microbiology 2023Quote: ... Then the different pellets were resuspended for 30 min in denaturing buffer (20 mM Tris, 500 mM NaCl, 6 M Guanidine, 20 mM Imidazole and protease inhibitor cocktail (Roche), pH 7.5) ...