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Citations for Roche :
351 - 400 of 1277 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... Then one hundred nanograms of RNA per sample were used to prepare dual-indexed strand-specific cDNA libraries using the KAPA mRNA HyperPrep kit (Roche). The resulting libraries were assessed for quantity and size distribution using Qubit and Agilent 2100 Bioanalyzer ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µg of total RNA was converted to a sequence library using a KAPA Stranded mRNA-seq Kit (KAPA Biosystems). These libraries were analyzed using a HiSeq 2500 sequencer (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... Relative mRNA expression was quantified using FastStart SYBR Green master mix on the LightCycler 96 instrument (Roche Life Science, USA) with specific primer pairs (Supplementary Table 9).
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bioRxiv - Cancer Biology 2021Quote: ... RNA was extracted for tissues with sufficient tissue and bulk RNAseq libraries were prepared with KAPA mRNA HyperPrep kit (Roche). Bulk RNA sequencing data was processed with the same pipeline as the GLASS samples.
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bioRxiv - Cancer Biology 2021Quote: ... libraries were prepared in an automated fashion using an Agilent Bravo robot with aKAPA Standard mRNA-Seq Kit (KAPA BIOSYSTEMS). In-house sequencing adaptors were ligated to 100-300 bp fragments of input dsDNA ...
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bioRxiv - Immunology 2021Quote: ... Poly A mRNA was purified from total RNA using oligodT magnetic beads and strand-specific indexed libraries were prepared using the Illumina’s TruSeq Stranded mRNA Sample Prep Kit followed by ten cycles of amplification using KAPA HiFi DNA polymerase (KAPA Biosystems). Libraries were quantified and pooled based on a post-PCR Bioanalyzer and 75 base pair (bp ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-Seq library was prepared according to KAPA mRNA HyperPrep kit with 200-300 bp insert size (Roche, Wilmington, MA) using 250 ng of total RNAs as input ...
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bioRxiv - Biochemistry 2022Quote: ... 150 ng of U1 RNA (TMG or NAD cap) and 150 ng of HIV-1 mRNA were mixed with ResoLight dye (1x, Roche) and annealing buffer (10 mM Tris ...
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bioRxiv - Genomics 2022Quote: ... RNA Libraries were prepared from 100 ng of total RNA with the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems KK8401). Indexed libraries were then pooled and 2×50 bp ...
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bioRxiv - Neuroscience 2022Quote: ... Mef2a mRNA expression was then assessed by qPCR (PowerTrack SYBR Green Master Mix, Applied Biosystems, LightCycler 480 Instrument II, Roche), along with that of 36b4 mRNA for data normalization ...
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bioRxiv - Cell Biology 2022Quote: ... RNA-Seq library was prepared according to KAPA mRNA HyperPrep kit with 200-300 bp insert size (Roche, Wilmington, MA) using 250 ng of total RNAs as input ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was used to generate the sequencing libraries using the KAPA Stranded mRNA Seq kit for the Illumina sequencing platform according to the manufacturer’s protocol (KAPA biosystems). Libraries were sequenced on an Illumina NextSeq 550 using 150 nt paired-end sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were constructed using ∼50 ng of mRNA using the Kapa Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, Illumina). Indexes were designed according to TruSeq RNA UD specifications (#20020591 ...
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bioRxiv - Genomics 2022Quote: ... RNA-seq libraries were prepared using 1 μg of total RNA input using KAPA stranded mRNA-seq kit for Illumina (Roche) as per manufacturer’s instruction and paired-end sequenced on NovaSeq 6000 (Illumina).
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bioRxiv - Developmental Biology 2023Quote: RNA sequencing (RNAseq) libraries were prepared using 1 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2022Quote: ... or IRF4-R98AC99A variants was processed by use of the KAPA mRNA Hyper Prep Kit for Illumina Platforms (KK8580; Roche) and KAPA Single-Indexed Adapter Kit (KK8700 ...
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bioRxiv - Physiology 2023Quote: The mRNA level was then calculated using the comparative Δ Δct method using the LightCycler software (v.96 1.1, Roche; same hereafter for all qPCR experiments) ...
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bioRxiv - Physiology 2023Quote: The mRNA level was then calculated using the comparative Δ Δct method using the LightCycler software (v.96 1.1, Roche; same hereafter for all qPCR experiments) ...
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bioRxiv - Bioengineering 2023Quote: ... Adaptor-ligated double-stranded cDNAs that were synthesized from the mRNA libraries were then PCR-amplified for eight cycles with KAPA HiFi DNA Polymerase (Roche), quantitated by PCR and subsequently pooled in equimolar concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... The Van Andel Genomics Core prepared RNA libraries from 500ng of total RNA using a KAPA mRNA Hyperprep kit (v4.17, Kapa Biosystems). RNA was sheared to 300-400bp ...
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bioRxiv - Genomics 2023Quote: ... The batches of isolated RNA were arranged sequentially in 96-well plates for quality control and preparation of libraries using the standard automated KAPA stranded mRNA library preparation protocol (Roche). One sample was lost in transport before library preparation (HG03478 (MSL)) ...
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bioRxiv - Neuroscience 2024Quote: ... RNAseq libraries were prepared by KAPA Stranded mRNA Sample Preparation Kit according to the manufacturer’s protocol (Kapa Biosystems, MA, USA) as previously described (Grabowska et al. ...
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bioRxiv - Developmental Biology 2024Quote: RNA-seq libraries were constructed by the ICM Platform (PARIS) from 100 ng of total RNA using the KAPA mRNA Hyperprep kit (Roche) that allows to obtain stranded polyA libraries ...
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bioRxiv - Neuroscience 2024Quote: ... and T7 promoter sequence was added on all the reverse primers for generating antisense mRNA probes using T7 RNA polymerase (Roche). For immunohistochemistry (IHC ...
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bioRxiv - Developmental Biology 2021Quote: ... and samples with RIN >7 were used for paired-end sequence library construction using the KAPA mRNA HyperPrep Kit (Roche # 08098123702). For ChIP samples ...
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bioRxiv - Developmental Biology 2019Quote: ... Sample preparation protocol for the RNASeq libraries was following the manufacturer’s recommendations of KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche-Kapa Biosystems). The libraries were sequenced on HiSeq2500 (Illumina ...
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bioRxiv - Developmental Biology 2019Quote: ... The purified products served as a template to synthesize digoxigenin-labelled antisense mRNA probes using DIG RNA labelling mix (Roche #11277073910).
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bioRxiv - Cancer Biology 2022Quote: ... libraries were prepared in an automated fashion using an Agilent Bravo robot with a KAPA Standard mRNA-Seq Kit (KAPA BIOSYSTEMS). In house adaptors were ligated to 100-300 bp fragments of dsDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 µg of purified RNA per sample was used to generate sequencing libraries following the manufacturer’s protocols of KAPA mRNA HyperPrep Kit (KK8581, Roche Life Science). The libraries were indexed using KAPA Unique Dual-Indexed Adapter Kit at 7 µM (KK8727 ...
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bioRxiv - Molecular Biology 2020Quote: ... Poly-A enriched libraries were generated using the Illumina TruSeq Standard mRNA preparation kit and checked via the Bioanalyzer and quantitative PCR (KAPA Biosystems). Paired-end reads (100 nt ...
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bioRxiv - Cancer Biology 2020Quote: ... Individual sample mRNA levels were analysed in triplicate in a 96-well plate using an LC480 light cycler instrument (Roche Diagnostics). Gene expression levels were normalised against 18S.
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bioRxiv - Developmental Biology 2022Quote: ... 100ng of total RNA were then sent to the Harvard University Bauer Core Facility for library preparation with the Kapa mRNA Library Prep kit (Roche, Basel). Generated RNA-seq libraries were then sequenced on a single sequencing lane of the Illumina NextSeq 500 using 2×38bp reads at the Harvard University Bauer Core Facility – see Table S1 for per-sample sequencing information ...
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bioRxiv - Developmental Biology 2020Quote: ... Strand-specific polyA-enriched libraries were prepared using the Kapa mRNA-seq Hyper kit (Kapa Biosystems, Cape Town, South Africa KK8581). Untreated samples were sequenced on an Illumina HiSeq4000 with 1×100bp reads ...
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bioRxiv - Molecular Biology 2019Quote: ... A qPCR analysis of mRNA expression from FD cells samples were conducted using KAPA SYBR Fast qPCR master mix (Kapa Biosystems) in a StepOne plus thermocycler PCR machine (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... and the mRNA content was quantified by real-time PCR using the LightCycler® 96 System (Roche Diagnostics K.K., Tokyo, Japan) with a KAPA SYBR FAST One-Step qRT-PCR Kit (Nippon Genetics Co. ...
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bioRxiv - Neuroscience 2019Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... cDNA libraries were prepared from poly(A)-captured mRNA using a KAPA RNA HyperPrep Kit (Roche, Basel, Switzerland, Cat No. KK8541) with Truseq adapters ...
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bioRxiv - Plant Biology 2022Quote: ... RT was performed with 5 µg of total mRNA using Transcriptor Reverse Transcriptase and oligo (dT)15 primer (Roche, Mannheim, Germany). QRT-PCR was run on a LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2020Quote: ... free of contaminating DNA) were directed for library preparation using Kapa Stranded mRNA Library Preparation Kit (Roche Diagnostics Corporation, Indiana, USA) and sequencing in an Illumina HiSeq 4000 platform ...
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bioRxiv - Genetics 2019Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Bulk RNA-seq libraries were prepared from 500 ng of total RNA for each sample using KAPA mRNA HyperPrep Kit for Illumina (Roche, #KR1352) following manufacturer’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... The libraries were constructed at the Genomics Core Facility at West Virginia University using the KAPA mRNA Stranded Kit (KAPA Biosystems), and then 150-bp paired-end sequenced at the BioHPC Lab at Cornell University using an Illumina NextSeq 500 platform ...
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bioRxiv - Systems Biology 2022Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Library construction of 300 ng total RNA for each sample was made using KAPA Stranded mRNA-Seq Kit (Illumina) (Kapa Biosystems), using 10 cycles of PCR amplification ...
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bioRxiv - Immunology 2022Quote: ... mRNAseq libraries were constructed using KAPA RNA HyperPrep Kit with RiboErase (HMR) Globin in conjunction with the KAPA mRNA Capture Kit (Roche Sequencing). Libraries were sequenced on an Illumina NextSeq500 sequencer using Illumina NextSeq 500/550 High Output v23 (150 cycles ...
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bioRxiv - Systems Biology 2023Quote: ... RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed by amplifying 500 ng of total RNA using 9 PCR cycles with the KAPA mRNA HyperPrep Kit (KAPA Biosystems). cDNA libraries were validated using the Bioanalyzer 2100 on a High Sensitivity DNA assay and quantified using the KAPA Library Quantification Kit for Illumina Platforms on a Roche Lightcycler 480 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... RNA libraries for sequencing were prepared using a KAPA Stranded mRNA Sample Preparation Kit (KK8401-07962169001, Kapa Biosystems, Wilmington, MA, USA). The libraries were sequenced after onboard cluster generation using HiSeq Rapid SBS Kit v2 (200 cycles ...