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Citations for Roche :
351 - 400 of 1129 citations for L Leucine 3 4 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The experimental cells were collected in a lysis buffer (0.5% SDS, 0.04 mol/L DTT, pH 7.5) supplemented with the protease inhibitor EASYpacks (Roche, Germany). The lysates were diluted with 3 × loading buffer (187.5 mmol/L Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... Lactate was quantified using a lactate assay kit (D-Lactic/L-Lactic acid UV method, r-biopharm, Roche). All samples were measured using fresh growth medium as control.
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bioRxiv - Developmental Biology 2019Quote: ... then incubated overnight at 4°C with anti-DIG antibody (Roche #11093274910) at 1:5000 in block ...
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bioRxiv - Cancer Biology 2021Quote: ... and 4 μL of 500 ug/ml RNase (Roche, Cat. No 11119915001) was added and incubated for 1 h at 37° C to digest RNA ...
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bioRxiv - Neuroscience 2021Quote: ... and visualized by staining with 4-nitro blue tetrazolium (NBT, #11383213001, Roche), 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Neuroscience 2020Quote: Animals were perfused and post-fixed overnight using 4% paraformaldehyde (HistoFix, Roche). Vibratome sections (100-200 μm ...
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bioRxiv - Immunology 2021Quote: ... Live/dead cell exclusion was performed with DAPI (4′-phenylindole dihydrochloride; Roche) or LIVE/dead fixable dye (Thermofisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with 4 μl of DIG-Prime kit (Roche, Mannheim, Germany), overnight at 37°C ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... overnight at 4 °C in presence of protease inhibitor cocktail (Roche, Switzerland). The beads were washed with buffer W ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 mM DTT) supplemented with 2x Complete EDTA free protease inhibitor (Roche). Samples were spun at 30,000 g for 10 min at 4°C and the cleared lysate was incubated with 40 µl anti-FLAG agarose bead slurry (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... proteinase K (4 µg/ml; F. Hoffmann La Roche Ltd, 211 Switzerland) and by heating at 96°C for 20 min in EnVisionTM FLEX Target Retrieval solution ...
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bioRxiv - Molecular Biology 2023Quote: ... were incorporated by PCR (4 cycles) using KAPA HiFi Hotstart ReadyMix (Roche).
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were counterstained with 4’,6-Diamidine-2’-phenylindole-dihydrochloride (DAPI; Roche).
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bioRxiv - Genomics 2023Quote: ... 4 μL of Lysis mix (Proteinase K (5.05 mg/mL, Roche, 3115879001), IGEPAL CA-630 (5.05% ...
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bioRxiv - Molecular Biology 2023Quote: ... nuclei were stained using 4’,6-diamidino-2-phenylindole (DAPI, Roche Diagnostics) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... 250 μL lysis buffer (4% w/v SDS and cOmplete Tablets (Roche) in 50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... then incubated overnight at 4°C with anti-DIG antibody (11093274910, Roche) at 1:5000 in blocking buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... and 5 mM MgCl2) containing 4-nitro blue tetrazolium chloride (NBT, Roche), 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Microbiology 2019Quote: ... beads containing bound methylated DNA were resuspended in 250 μl digestion buffer (50 mM Tris pH 8, 10 mM EDTA, 0.5% SDS) with 100 μg Proteinase K (Roche) and incubated at 65°C for 4 h ...
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bioRxiv - Biochemistry 2022Quote: ... The cell pellet collected from 6 L growth was resuspended in 20 mM HEPES (pH 7.8) containing protease inhibitor cocktail (Roche) and DNase I (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were resuspended and bead beaten in 250 μL urea buffer (6 M urea, 1% SDS, 50 mM Tris-HCl pH 6.8, 1 mM EDTA, 2x Roche protease inhibitors ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM ATP and 10% glycerol) containing 1 mmol/L PMSF and DNase I (Roche Diagnostics Corp., IN, USA) to 5 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and incubated in buffer A (50 mM MMT pH 8.0/300 mM KCl/10 mM MgCl2/5 % glycerol) containing 1 g L-1 lysozyme/2.5 U mL-1/SmDNAse//complete protease inhibitor cocktail (Roche) for 1 h prior to lysis using EmulsiFlex C5 (Avestin ...
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bioRxiv - Plant Biology 2021Quote: ... and 250 µl extraction buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 % Nonidet P40, proteinase inhibitor cocktail (Roche)) per 200 mg tissue powder was added ...
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bioRxiv - Cell Biology 2021Quote: ... The washed beads were resuspended in 200 μL 50 mM ammonium bicarbonate (pH 8.3) and on-bead trypsin digest (rolling with 1 μg of trypsin (Roche) for 16 h at 37°C followed by a second trypsin digest with 0.5 μg trypsin for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... were mixed with an equal volume of 100 mmol/L Tris-HCl (pH 7.4) – 2% sarkosyl for 30 minutes prior to PK (200 μg/mL; Roche) digestion for 1 h at 37° C ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... Hematocrit (0.5 to 0.64) and blood glucose concentration (5.7 to 8.6 mmol/L, Accu-Chek Performa Nano, Roche, Mannheim, GER) were measured shortly before injecting the radiotracer.
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bioRxiv - Plant Biology 2023Quote: ... in 100μl extraction buffer (Tris-HCl 50 mM pH 6.8, SDS 2%, DTT 2 mM and 1× protease inhibitors (Roche) and centrifuged for 5 min at 13,000g at 4OC ...
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bioRxiv - Molecular Biology 2023Quote: 3-5 day old males of the indicated genotypes and experimental groups were homogenised in 150µl of homogenisation buffer (0.2%Triton X-100 supplemented with 1 x complete mini EDTA-free protease inhibitor (Roche) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μl of the sample were treated for 30 min at 50°C with 1.2 mg/ml Proteinase K (Roche) in Proteinase K Buffer (100 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was washed twice with 300 μl sodium phosphate buffer (20 mM Na-phosphate pH 6.8, 1 mM PMSF, 1× Mini Protease Inhibitor Cocktail, Roche) with centrifugation at 16,000g for 20 min between washes ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed in 100μL or 200μl of 2% SDS lysis buffer (2% SDS, 50mM Tris-HCl pH 7.5, 5mM EDTA, 15U/mL DnaseI (Roche), cOmplete mini EDTA-free protease inhibitor tablet (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume). The suspension was frozen and stored at −80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume) were added to the cell suspension and stirred at RT for 30 mins ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached using cold PBS and lysed in 350 µl lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor (Roche) for at least 20 min on ice ...
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bioRxiv - Neuroscience 2024Quote: ... underwent lysis through the addition of 200 µl of a 50 mM Tris-HCl buffer (pH 7.8) containing 5% SDS and cOmplete ULTRA protease inhibitor (Roche). The samples were then subjected to Bioruptor® treatment (Diagenode ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were blocked for 1 hr at room temperature (RT) in 3% BSA (Roche; 10735086001), 0.05% Tween (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... 3 % SDS and 5 mM EDTA) supplemented with 1x complete protease inhibitor cocktail (Roche, Switzerland) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Biochemistry 2020Quote: ... samples were then digested for a further 3 hrs with 2 μg Chymotrypsin (Roche 11418467001) at 25°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then pelleted and washed 3 times with PBS containing protease inhibitor (Roche, 11873580001). The pellets were snap-frozen and stored at -80°C for later use ...
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bioRxiv - Immunology 2021Quote: ... 3 × 104 HMEC-1 cells were seeded into an E-16 multi-well plate (Roche) in triplicate and incubated for 72 h ...
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bioRxiv - Microbiology 2019Quote: ... frozen pellets were thawed and resuspended in 1 ml of 3 mg/ml lysozyme (Roche) and 0.4 mg/ml proteinase K (Ambion ...
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bioRxiv - Cell Biology 2020Quote: ... as well as AP substrate consisting of 5-bromo-3-chloro-indolyl phosphate (Roche Diagnostics) and 4-nitro blue tetrazolium chloride (Roche Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed at 3 days in ice-cold RIPA buffer containing protease inhibitors (Roche) and protein concentration was determined by BCA Assay (Gibco BRL ...
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bioRxiv - Genomics 2019Quote: ... beads were washed 3×1mL with bead wash buffer (1x PBS, 5mg/mL BSA, Roche complete EDTA-free protease inhibitor ...
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bioRxiv - Molecular Biology 2022Quote: To determine the repM transcription start site a 2nd Generation 5’/3’ RACE Kit (Roche) was used according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... Primer2: 5’-CAAGCAGAAGACGGCATACGA*G-3’) and 15µl 2x Kapa HiFi HotStart Ready Mix (Kapa Biosystems), amplification was performed for 45 s at 98°C ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were then prepared from 3 ng DNA with the Kapa HyperPrep Kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Physiology 2023Quote: ... 3 µL of Light Cycler 480 SYBR® Green I Master mix (Roche Diagnostics, Switzerland), 0.24 µL of each primer (10X ...