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351 - 400 of 2715 citations for Goat anti mouse HRP secondary antibody since 2020

• The blast pathogen effector AVR-Pik binds and stabilizes rice heavy metal-associated (HMA) proteins to co-opt their function in immunity

  K. Oikawa, et al., bioRxiv - Plant Biology 2020

  Quote: ... Proteins were immunologically detected by using anti-HA (3F10)-HRP (Roche, Switzerland) or anti-Myc-tag (HRP-DirecT ...
• A rate threshold mechanism regulates MAPK stress signaling and survival

  Amanda N. Johnson, et al., bioRxiv - Systems Biology 2020

  Quote: ... HAx3-Ptp2 was detected using an anti-HA HRP conjugate 3F10 (Roche) at a 1:5,000 dilution ...
• Zinc dysregulation in slc30a8 (znt8) mutant zebrafish leads to blindness and disrupts bone mineralisation

  Eirinn W Mackay, et al., bioRxiv - Physiology 2020

  Quote: ... Probes were detected with HRP anti-Dig Fab (Roche Diagnostics, Mannheim, Germany).
• CMTr cap-adjacent 2’-O-ribose mRNA methyltransferases are required for reward learning and mRNA localization to synapses

  Irmgard U. Haussmann, et al., bioRxiv - Molecular Biology 2021

  Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
• Oriented Soft DNA Curtains for Single Molecule Imaging

  Aurimas Kopūstas, et al., bioRxiv - Biophysics 2020

  Quote: ... Anti-Dig antibodies (Roche). Buffer solutions ...
• GENOMES UNCOUPLED1: an RNA-binding protein required for early PSII biogenesis

  Chaojun Cui, et al., bioRxiv - Plant Biology 2023

  Quote: ... All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche; anti-D2 antibody from Agrisera). Coomassie brilliant blue (CBB ...
• Design of a new effector recognition specificity in a plant NLR immune receptor by molecular engineering of its integrated decoy domain

  Stella Cesari, et al., bioRxiv - Plant Biology 2021

  Quote: ... Membranes were blocked in 5% skimmed milk and probed with anti-HA (Roche anti-HA-HRP 3F10), anti-Myc mouse monoclonal antibodies (Roche ...
• Selective axonal translation of prenylated Cdc42 mRNA isoform supports axon growth

  Seung Joon Lee, et al., bioRxiv - Neuroscience 2020

  Quote: ... and then incubated for one hour in donkey anti-mouse FITC antibody (1:200) in 0.3% Triton-X100 supplemented with 1X blocking buffer (Roche). After washing with PBS for 5 min ...
• Protein Detection and Localization in Plant Cells Using Spot-Tagging

  Andriani Mentzelopoulou, et al., bioRxiv - Plant Biology 2020

  Quote: ... roots were incubated overnight at 4°C in anti-myc-mouse primary antibody (1:250, Roche in blocking solution), washed 3 times in PBST ...
• Analysis of a Mad2 homolog in Trypanosoma brucei provides possible hints on the origin of the spindle checkpoint

  Bungo Akiyoshi, bioRxiv - Cell Biology 2020

  Quote: ... The supernatant was transferred into a new tube and was mixed with mouse monoclonal anti-GFP antibodies (11814460001, Roche) that had been pre-conjugated with Protein-G Dynabeads with dimethyl pimelimidate using a protocol described in (Unnikrishnan et al. ...
• ULK4 and Fused/STK36 interact to mediate assembly of a motile flagellum

  Ciaran J. McCoy, et al., bioRxiv - Cell Biology 2022

  Quote: ... with SDS-PAGE and immunoblots performed by standard methods using the following mouse monoclonal antibodies: anti-GFP (11814460001, Roche) and anti-MYC (clone 4A6 ...
• SPACA6 structure reveals a conserved superfamily of gamete fusion-associated proteins

  Tyler D.R. Vance, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Transfer membranes were then incubated with a 1:10,000 dilution of primary mouse anti-6xHis antibody (Roche, cat. #11922416001) for 1 h ...
• Methamphetamine enhances caveolar transport of therapeutic drugs across the rodent blood-brain barrier

  Jui-Hsien Chang, et al., bioRxiv - Neuroscience 2020

  Quote: ... Glioma graft cells were labeled by the anti-human Vimentin antibody (mouse monoclonal, V9, 790-2917, Roche, pre-diluted), followed by a mouse secondary antibody (rabbit polyclonal anti-mouse biotin antibody ...
• Intra-axonal translation of Khsrp mRNA slows axon regeneration by destabilizing localized mRNAs

  Priyanka Patel, et al., bioRxiv - Neuroscience 2020

  Quote: ... and then incubated for in donkey anti-mouse FITC antibody diluted in 10 X blocking buffer (1:100; Roche) + 0.3 % Triton-X100 for 1 h ...
• Plastid-specific RsmD methyltransferase and ribosome maturation factor RimM are crucial for 16S rRNA maturation and proteostasis

  Kaiwei Liu, et al., bioRxiv - Plant Biology 2022

  Quote: ... and the interaction between AtRsmD and the selected proteins was determined by immunoblot analysis with a mouse anti-GFP monoclonal antibody (1:5,000; Roche) and a mouse anti-Myc monoclonal antibody (1:10,000 ...
• Comparative proteomics of vesicles essential for the egress of Plasmodium falciparum gametocytes from red blood cells

  Juliane Sassmannshausen, et al., bioRxiv - Molecular Biology 2023

  Quote: ... membranes were incubated overnight at 4°C with monoclonal mouse anti-GFP antibody (Roche; Basel, CH; dilution 1:500) or monoclonal rat anti-HA antibody (Roche ...
• A calcineurin-mediated scaling mechanism that controls a K⁺-leak channel to regulate morphogen and growth factor transcription

  Chao Yi, et al., bioRxiv - Developmental Biology 2021

  Quote: ... The secondary antibody solution was then replaced with DAPI solution (Roche, 10236276) in the dark at room temperature for 5 min ...
• Autoantibodies against Progranulin and IL-1 receptor antagonist due to immunogenic posttranslational isoforms contribute to hyperinflammation in critically ill COVID-19

  Lorenz Thurner, et al., bioRxiv - Immunology 2021

  Quote: ... corresponding biotinylated antihuman Fab secondary antibodies and subsequently peroxidase-labeled streptavidin (Roche) were used.
• A pigtailed macaque model for Kyasanur Forest disease virus and Alkhurma hemorrhagic disease virus

  Rebecca M. Broeckel, et al., bioRxiv - Microbiology 2021

  Quote: ... Secondary antibodies include Discovery Red 610 (Roche Tissue Diagnostics cat#760-245), Discovery FITC (Roche Tissue Diagnostics cat#760-232) ...
• Establishment and characterization of a tumoroid biobank derived from dog patients’ mammary tumors for translational research

  Antonella Raffo-Romero, et al., bioRxiv - Cancer Biology 2022

  Quote: ... secondary antibodies and the Discovery ChromoMap DAB kit reagents (760–159, Roche). Counterstaining and post-counterstaining were performed using hematoxylin and bluing reagent (Ventana ...
• A protein hydroxylase couples epithelial membrane biology to nucleolar ribosome biogenesis

  Eline Hendrix, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary-secondary-antibody pairs were as follows: Ki67 (Roche Tissue Diagnostics, 05278384001), OmniMap-anti-Rb HRP (Roche Tissue Diagnostics) ...
• The C. elegans ASPP homolog APE-1 is a junctional protein phosphatase 1 modulator

  Gwendolyn M. Beacham, et al., bioRxiv - Cell Biology 2022

  Quote: ... and rat anti-HA directly conjugated to HRP (1:500, clone 3F10, Roche). Secondary antibodies and dilutions included goat anti-mouse IRDye 800CW (1:20000 ...
• The wheat NLR protein PM3b localizes to endoplasmic reticulum-plasma membrane contact sites and interacts with AVRPM3^b2/c2 through its LRR domain

  Jonatan Isaksson, et al., bioRxiv - Plant Biology 2024

  Quote: ... followed by detection using HRP conjugated primary anti-HA (clone 3F10, 12013819001, Roche) and HRP conjugated primary anti-GFP (sc-9996 HRP ...
• The wheat NLR protein PM3b localizes to endoplasmic reticulum-plasma membrane contact sites and interacts with AVRPM3^b2/c2 through its LRR domain

  Jonatan Isaksson, et al., bioRxiv - Plant Biology 2024

  Quote: ... Membranes were probed with HRP conjugated primary anti-HA (clone 3F10, 12013819001, Roche), anti-ATPase (AS07 260 ...
• A conserved amino acid in the C-terminus of HPV E7 mediates binding to PTPN14 and repression of epithelial differentiation

  Joshua Hatterschide, et al., bioRxiv - Microbiology 2020

  Quote: ... HA-tagged proteins were detected and visualized using an HA antibody conjugated to HRP (Roche). For anti-HA immunoprecipitations ...
• SARS-CoV-2 spike-glycoprotein processing at S1/S2 and S2’and shedding of the ACE2 viral receptor: roles of Furin and TMPRSS2 and implications for viral infectivity and cell-to-cell fusion

  Rachid Essalmani, et al., bioRxiv - Biochemistry 2021

  Quote: ... the proteins were detected using an HA-HRP antibody (12-013-819; 1:3,500; Roche).
• A Receptor Like Cytoplasmic Kinase evolved in Aeschynomene legumes to mediate Nod-independent rhizobial symbiosis

  Natasha Horta Araújo, et al., bioRxiv - Plant Biology 2024

  Quote: ... The following antibodies were used for protein biochemistry experiments: αHA-Hrp (12013819001, Roche, 1/5000), αFlag-Hrp (A8592 ...
• “TMEM16K is an interorganelle regulator of endosomal sorting”

  Maja Petkovic, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-GFP (Roche, # 11814460001, 1/1000), rabbit anti-calreticulin (Abcam ...
• Meiotic sister chromatid exchanges are rare in C. elegans

  David E. Almanzar, Spencer G. Gordon, Ofer Rog, bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-GFP (1:1000, Roche 11814460001), goat anti-SYP-1 (1:500 ...
• Substrate-induced clustering activates Trim-Away of pathogens and proteins

  Jingwei Zeng, et al., bioRxiv - Molecular Biology 2020

  Quote: ... mouse anti-GFP (7.1+13.1, Roche, 11814460001) and normal rabbit IgG (Millipore 12-370).
• The m6A landscape of polyadenylated nuclear (PAN) RNA and its related methylome in the context of KSHV replication.

  Sarah Elizabeth Martin, et al., bioRxiv - Molecular Biology 2021

  Quote: ... primary mouse anti-DIG (Sigma Roche 11333062910) was added at the dilution of 1 ...
• Regulation of KHNYN antiviral activity by the extended di-KH domain and nucleo-cytoplasmic trafficking

  María José Lista, et al., bioRxiv - Microbiology 2021

  Quote: ... 1:1000 anti-GFP (Mouse. Roche 11814460001), 1:5000 anti-rabbit HRP (Cell Signaling Technology ...
• The 14-3-3 Proteins Bmh1 and Bmh2 Are Key Regulators of Meiotic Commitment

  Janardan N. Gavade, et al., bioRxiv - Cell Biology 2021

  Quote: ... 10µg anti-GFP mouse (Roche, Catalog #11814460001) and 1mL of NP-40 lysis buffer was added to the DYNAbeads tube ...
• A cooperative network at the nuclear envelope counteracts LINC-mediated forces during oogenesis in C. elegans

  Chenshu Liu, et al., bioRxiv - Cell Biology 2021

  Quote: ... anti-GFP (mouse monoclonal, Roche, 1:400), anti-SYP-1 (goat ...
• CIGB-300 peptide targets the CK2 phospho-acceptor domain on Human Papillomavirus E7 and disrupts the Retinoblastoma (RB) complex in cervical cancer cells

  Ailyn C. Ramón, et al., bioRxiv - Cancer Biology 2022

  Quote: ... mouse monoclonal anti-HA (1: 500; Roche), mouse monoclonal anti-HPV-16 E7 (1 ...
• PCDH7 Promotes Cell Migration by Regulating Myosin Activity

  Mohammad Haroon Qureshi, et al., bioRxiv - Cell Biology 2022

  Quote: ... anti-GFP mouse (11814460001, Roche 1:1000), anti-MYPT1 mouse (sc-514261 ...
• Heterodimers of functionally divergent ARF-GEF paralogues prevented by self-interacting dimerisation domain

  Sabine Brumm, et al., bioRxiv - Plant Biology 2021

  Quote: ... mouse anti-GFP (Roche catalogue no 11814460001), 1:2500 ...
• A nuclear export signal in KHNYN required for its antiviral activity evolved as ZAP emerged in tetrapods

  Maria Jose Lista, et al., bioRxiv - Microbiology 2022

  Quote: ... 1:1000 anti-GFP (Mouse. Roche 11814460001), 1:5000 anti-rabbit HRP (Cell Signaling Technology ...
• Structural basis for the unique multifaceted interaction of DPPA3 with the UHRF1 PHD finger

  Keiichi Hata, et al., bioRxiv - Biochemistry 2022

  Quote: ... mouse anti-GFP (monoclonal; 1:5000; Roche), goat anti-rabbit HRP (polyclonal ...
• Auto-inhibition of Cnn binding to γ-TuRCs prevents ectopic microtubule nucleation and cell division defects

  Corinne A. Tovey, et al., bioRxiv - Cell Biology 2021

  Quote: ... using anti-GFP (1:250) (mouse, Roche) and anti-gamma-Tubulin (1:250 ...
• Modulation of the promoter activation rate dictates the transcriptional response to graded BMP signaling levels in the Drosophila embryo

  Caroline Hoppe, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-biotin (1:250 Roche, 1297597), donkey anti-Sheep IgG Secondary Antibody ...
• Poly(ADP-ribose) glycohydrolase promotes formation and homology-directed repair of meiotic DNA double-strand breaks independent of its catalytic activity

  Eva Janisiw, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse monoclonal anti GFP (Roche, 1:500), guinea pig polyclonal anti HTP-3 (1:500 ...
• Astroglial calcium transfer from endoplasmic reticulum to mitochondria determines synaptic integration

  Roman Serrat, et al., bioRxiv - Neuroscience 2020

  Quote: ... mouse anti-myc (1:500; Roche, 11667149001), rabbit anti p-AKT (T296 ...
• A drug repurposing screen identifies altiratinib as a selective inhibitor of a key regulatory splicing kinase and a potential therapeutic for toxoplasmosis and malaria

  Christopher Swale, et al., bioRxiv - Microbiology 2021

  Quote: ... mouse anti-HA tag (Roche, RRID: AB_2314622), and rabbit anti-HA Tag (Cell Signaling Technology ...
• LENG8 regulation of mRNA processing, is responsible for the control of mitochondrial activity

  Yongxu Zhao, et al., bioRxiv - Cell Biology 2021

  Quote: ... the mouse anti-GFP were from Roche; the mouse anti-FLAG was from Genscript (Nanjing ...
• A plant-like mechanism coupling m6A reading to polyadenylation safeguards transcriptome integrity and developmental genes partitioning in Toxoplasma

  Dayana C. Farhat, et al., bioRxiv - Microbiology 2021

  Quote: ... mouse anti-HA tag (Roche; RRID, AB_2314622), rabbit anti-HA Tag (Cell Signaling Technology ...
• Aquaporin regulates cell rounding through vacuole formation during endothelial-to-hematopoietic transition

  Yuki Sato, et al., bioRxiv - Developmental Biology 2022

  Quote: ... mouse anti-GFP (1:1,000 dilution, Roche), rabbit anti-GFP (1:1,000 dilution ...
• KDM2B regulates hippocampal morphogenesis by transcriptionally silencing Wnt signaling in neural progenitors

  Bo Zhang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... mouse-anti-BrdU (1:500; Roche, 11170376001), rabbit-anti-DCX (1:500 ...
• In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming

  Ana Vera Antunes, et al., bioRxiv - Microbiology 2023

  Quote: ... mouse anti-HA tag (Roche, RRID: AB_2314622), rabbit anti-HA Tag (Cell Signaling Technology ...
• Plant TIR domains physically interact with EDS1 family proteins to propagate immune signalling

  Jian Chen, et al., bioRxiv - Plant Biology 2023

  Quote: ... or mouse anti-GFP (Roche, REF 11814460001) and anti-Myc antibodies (Roche ...