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Citations for Roche :
351 - 400 of 5091 citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The real-time qPCR primers were designed using the universal probe library assay design center (Roche) and UCSC Genome Bioinformatics (table 3) ...
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bioRxiv - Genetics 2023Quote: ... or TaqMan gene expression assays (see details below) on the LightCycler 480 (Roche Diagnostics, Mannheim, Germany). Human tissue was obtained from the NIH NeuroBioBank’s Brain and Tissue repository at the University of Maryland ...
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bioRxiv - Biochemistry 2023Quote: ... Denaturation assays were performed in a 384-well plate in a qPCR machine (Roche Lightcycler II) using a temperature range of 25-99 °C ...
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bioRxiv - Genomics 2022Quote: ... High Sensitivity DNA Analysis Kit and KAPA SYBR FAST qPCR Kit (Roche). WGS was performed on the HiSeq X (HCS HD 3.5.0.7 ...
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bioRxiv - Cancer Biology 2022Quote: ... RNase free kit (Roche). RNA amount was quantified and cDNA was prepared using TaqMan Reverse Transcription Reagents (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cells were lysed in Radioimmunoprecipitation assay buffer (RIPA) buffer with the addition of protease (Roche cOmplete), and phosphatase inhibitors (GB Sciences) ...
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bioRxiv - Immunology 2021Quote: Macrophage cell lysates were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor cocktails and PhosSTOP (Roche). Protein concentration was measured using the BCA Protein Assay Kit (Pierce) ...
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bioRxiv - Immunology 2021Quote: ... The primers were designed using the Roche Universal Probe Library (UPL) Assay Design tool (Roche, Basel, Switzerland) with the indicated UPL probes ...
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bioRxiv - Immunology 2022Quote: Plasma viral loads were assessed using Amplicor HIV-1 Monitor Ultrasensitive Assay (Roche Molecular System, Branchburg, NJ).
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bioRxiv - Cell Biology 2020Quote: ... and LacZ activity was measured using a liquid β-galactosidase assay employing chlorophenolred-ß-D-galactopyranoside (Roche) as a substrate.
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was evaluated using a colometric formazan-based cell viability assay (WST-1; Roche, Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... The reverse transcriptase activity of the viruses was measured by using the colorimetric Reverse Transcriptase Assay (Roche).
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bioRxiv - Microbiology 2020Quote: A colorimetric assay based on the measurement of LDH activity released by damaged cells was used (Roche). Experiments were performed in 96-well plate and the manufacturer’s instructions were followed ...
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bioRxiv - Pathology 2019Quote: ... Frozen tissues were homogenized and lyzed in radioimmunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitors (Roche) followed by centrifugation ...
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bioRxiv - Plant Biology 2020Quote: ... Gene primers and probes were designed using Universal Probe Library (UPL) assay design centre (Roche Diagnostics Ltd.). RT-qPCR was performed using FastStart Universal Probe Master Mix (Roche ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cell viability was measured by trypan blue exclusion or WST-1 assays (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Physiology 2020Quote: ... The plasma was separated and stored at −80°C for insulin assay according to manufacturer’s instructions (Roche).
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bioRxiv - Microbiology 2020Quote: ... The immunohistochemistry (IHC) assay was carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
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bioRxiv - Genetics 2021Quote: ... which was performed with LightCycler 480 SYBR Green І Master Gene Expression Assays (Roche Molecular BiocheMHcals, Germany), is quantitated for the expression of β-actin/Akt/mTOR/p70S6K/eIF4E by employing the 2−ΔΔCT value models [31] ...
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells using Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X cOmplete Protease InhibitorCocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Genomics 2020Quote: ... This final probe set was used for the preparation of a SeqCap EZ Developer assay by Roche NimbleGen Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... For LC-MS/MS based experiments the kinase assay buffer contained 80 μM cold ATP (10519979001, Roche)) ...
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bioRxiv - Genetics 2022Quote: ... and the barcoded sequencing libraries were quantified using a standard curve-based qPCR assay (KAPA Biosystems, USA) and Agilent Bioanalyzer 2100 (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: Radioimmunoprecipitation Assay (RIPA) buffer and cOmplete mini-proteinase inhibitors (1 tablet per 10 ml extraction solution, Roche) was used to homogenize striatal tissue and cells ...
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bioRxiv - Molecular Biology 2022Quote: Cell lysates were prepared in radioimmunoprecipitation assay buffer supplemented with protease inhibitor cocktail tablets (Roche, Basel, Switzerland), and total protein was measured using a Bicinchoninic Acid Protein Assay Kit (Beyotime Biotechnology ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Total protein was extracted with radio immune precipitation assay (RIPA) buffer containing phenylmethylsulphonyl fluoride (PMSF) (Roche, Switzerland) and a protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2023Quote: ... followed by library preparation with KAPA RNA HyperPrep kit (Roche, kit code KK8541), according to manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... EDTA/DTT-treated IECs were lysed in radioimmunoprecipitation assay buffer (RIPA) supplemented with protease and phosphatase inhibitors (Roche). Equal volumes of 2X Laemmli buffer (Sigma-Aldrich ...
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bioRxiv - Physiology 2019Quote: ... The qPCR assays were performed as previously described (37) using the LightCycler96 with SYBR Green I (Roche, Switzerland). All qPCR assays were run in duplicate on cDNA diluted 1:5 ...
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bioRxiv - Cancer Biology 2019Quote: Snap-frozen tumor samples were homogenized in radio immunoprecipitation assay buffer with cOmpleteTM Protease Inhibitor Cocktail Tablets (Roche) and phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: Cell viability was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Roche, Mannheim, Germany), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μl 1xTaqMan Gene Expression Assay primer and 10 μl 1x FastStart Universal Probe Master-mix (Roche, 04913957001) were applied ...
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bioRxiv - Cancer Biology 2021Quote: ... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (11465007001, Roche Diagnostics, Mannheim, Germany) was performed according to manufacturer’s instructions ...
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bioRxiv - Epidemiology 2020Quote: ... Real-time PCR assays were performed with LightCycler® 480 Probe Master Mix 1× (Roche Applied Science, Germany), using 200 nM of primers and probes in a final volume of 12 µl and 2 µl of control DNA were added ...
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bioRxiv - Pathology 2020Quote: ... Radioimmunoprecipitation assay buffer (Biosesang, Inc., Seongnam, Korea) with a protease inhibitor cocktail (Roche Diagnostics, Indianapolis, IN,v USA) was prepared ...
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells in Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X Complete Protease Inhibitor Cocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation was assessed by WST-1 assay as per manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). Colony formation assay was performed with MethoCult methylcellulose-based media as per manufacturer’s protocol (Stemcell Technologies ...
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bioRxiv - Physiology 2020Quote: ... The expression of all genes was related to an internal housekeeping gene assay for Actb (Roche, Welwyn, UK) as described previously (O’Hara et al. ...
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bioRxiv - Cancer Biology 2021Quote: For HA pulldown assays the cells were lysed with 500 µL of IP Lysis Buffer containing cOmplete (Roche) protease inhibitor cocktail and incubated at 4°C for 35 min on a rotator followed by centrifugation at 15000 rpm for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The Roche Elecsys anti-SARS-CoV-2 assay was performed on Roche Cobas e411 (Roche Diagnostics, Indianapolis, IN). The Elecsys antinSARSnCoV-2 assay uses a recombinant protein representing the N antigen for the determination of antibodies against SARSnCoVn2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three replicates of each TaqMan® assay were run on a LightCycler® II 480 (Roche, Basel, Switzerland) real time PCR machine in a 384-well format ...
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells in Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X Complete Protease Inhibitor Cocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: The cytotoxicity assays were performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Roche, ref:11465007001) protocols according to the previous study [47] with few modifications ...
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bioRxiv - Genomics 2020Quote: ... Specimens in May were tested using the Cobas® SARS-CoV-2 RT-PCR assay (Roche Diagnostics, USA). Respiratory samples archived at the Clinical Laboratory ...
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bioRxiv - Neuroscience 2021Quote: ... PCR primers for the genes of interest were designed by the Universal Probe Library Assay Design Center (Roche) (primer sequences listed in key resource table).
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bioRxiv - Microbiology 2022Quote: ... DNA concentrations were estimated using qPCR and the Kapa Library Quantitation Assay for Illumina (Kapa Biosystems, Wilmington, MA). Genomic DNA was sequenced on an Illumina NovaSeq 600 sequencer (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... The enriched FLC-Venus protein was detected by western blot assay with the antibody anti-GFP (11814460001, Roche). Signals were visualized with chemiluminescence (34095 ...
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bioRxiv - Immunology 2023Quote: Total murine plasma cholesterol and triglyceride concentrations were measured with colorimetric assays (CHOD-PAP and CPO-PAP, Roche) using a microplate reader (Infinite F200 PRO ...
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bioRxiv - Immunology 2023Quote: ... CMV infection/reactivation was determined by pp65 antigenemia assays prior to 2010 and by CMV qPCR (Roche Diagnostics) from 2010 onward.
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bioRxiv - Pathology 2024Quote: Cholesterol and triglyceride levels were quantified in liver protein lysates and EDTA-plasma using enzymatic colorimetric assays (c.f.a.s. cobas, Roche Diagnostics) according to the manufacturer’s protocol ...