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Citations for Roche :
3751 - 3800 of 8559 citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were prepared by scraping cells using 1X lysis buffer (10X recipe-50 mM Tris pH 7.5, Triton 20%, NP40 10%, 2 M NaCl, mixed with cOmplete protease inhibitor tablet - Roche, Product number 11873580001). Cell lysates were rotated on a wheel at 4°C for 15 min and centrifuged for 10 min at 13,000 rpm 4°C to pellet the cell debris ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in buffer F (20 mM Tris pH 7.5, 100 mM NaCl, 5 mM MgCl2, 2 mM EGTA and Roche cOmplete protease inhibitor) and lysed by fluidizer ...
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bioRxiv - Immunology 2022Quote: Anti-SARS-CoV-2 (N protein) IgM/IgG levels in the serum were measured using an Elecsys Anti-SARS-CoV-2 with cobas8000 (Roche Diagnostics KK) at the Department of Clinical Laboratory ...
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bioRxiv - Cell Biology 2022Quote: ... and then homogenized on a gentleMACS Octo Dissociator (Miltenyi) using the “Protein_01_01” protocol with MACS buffer (5 mM CaCl2, 2 mM EDTA, 1X protease inhibitor (Roche, 05-892-970-001), 3 mM MgAc ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in lysis buffer (25 mM HEPES pH 7.5, 400 mM NaCl, 10 mM 2-mercaptoethanol, 0.1% Triton X-100 supplemented with lysozyme and Roche cOmplete protease inhibitor tablet), lysed by sonication (50% duty cycle ...
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bioRxiv - Microbiology 2019Quote: ... the ORF50 trans-complementing BHK-21 cells were transfected with 2 μg of ΔORF50 BAC DNA using FuGENE HD Transfection Reagent (Roche, Mannheim, Germany). When cells showed a visible CPE ...
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bioRxiv - Molecular Biology 2020Quote: ... The Dynabeads™ were washed once with 2 ml of PBS and coated with 15 μg of mouse monoclonal anti-GFP antibody (Roche Applied Science). The beads were finally washed three times with 2 ml of lysis buffer and added to the whole cell extract for a 2 h incubation with gentle rotation ...
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bioRxiv - Pathology 2021Quote: CCDs were dissected under stereomicroscopic observation either from fresh kidney slices (microperfusion) or after liberase treatment (Blendzyme 2, Roche Diagnostics, Meylan, France) (immunoblotting ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 2 mM MgCl2, 0.3 % Triton X-100, 0.25 % IGEPAL CA-630, 10 % glycerol, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 20 min followed by sonication using Diagenode Bioruptor for 10 min (30 sec ON ...
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bioRxiv - Cell Biology 2021Quote: A reference sample was generated by lysing TK6 cells in DPBS with 2% SDS and cOMPLETE protease inhibitors without EDTA (Roche, 1x concentration) at 70 °C ...
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were resuspended in Lysis Buffer (25 mM HEPES pH 8.0, 250 mM NaCl, 10 mM imidazole pH 8.0, 5 mM 2-mercaptoethanol, 10% glycerol and supplemented with Roche cOmplete protease inhibitor) and sonicated ...
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bioRxiv - Molecular Biology 2021Quote: ... the probe for RNA FISH was prepared with 2 µg of BAC RP183A17 using Nick translation kit (10976776001) purchased from Roche (Basel, Switzerland). Salmon sperm DNA and cot1 DNA were added to the probe to mask the repetitive sequence ...
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bioRxiv - Microbiology 2020Quote: ... was cut into small pieces of approximately 2 mm3 in a petri dish using a sterile scalpel and transferred into a 2 ml MagNA Lyser bead tube (Roche, United Kingdom). Following this ...
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bioRxiv - Genomics 2020Quote: ... The fixed tissue was washed twice for 2 minutes with icecold PBS plus 1x cOmplete™ mini Proteinase Inhibitor (PI) Cocktail (Roche, 11846145001). The tissue was minced by the Dounce Tissue Grinder (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... The 1st PCR was performed in a 12-μL total volume of reaction mixture containing 6.0 μL of 2 × KAPA HiFi HotStart ReadyMix (KAPA Biosystems, MA, USA), 0.72 μL of forward and reverse primer (10 μM) ...
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bioRxiv - Immunology 2022Quote: ... colon tissue was homogenised in 350 µl of lysis buffer (40 ml of PBS, 10%FCS, 2 Complete Protease Cocktail Inhibitor Tablets (Roche, Mannheim, Germany). Each sample was subject to 3 rounds of homogenisation using a MagnaLyser (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... were homogenized in 2 mL Precellys tube (Bertin Instruments) containing ceramic beads and 600 μL MagNaPure LC RNA Isolation Tissue buffer (Roche Life Science). The homogenate (400 μL ...
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Gut microbial disruption in critically ill patients with COVID-19 associated pulmonary aspergillosisbioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 infection was confirmed by quantitative reverse transcription PCR (TaqMan™-PCR performed on Roche cobas® 6800, Basel, Switzerland), performed on nasopharyngeal swabs or tracheal fluid ...
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bioRxiv - Cell Biology 2024Quote: ... Each reaction was then added to Strep-Tactin®XT 4Flow® high capacity resin (IBA # 2-5030-010) pre-equilibrated with XB buffer supplemented with cOmplete™ EDTA-free Protease Inhibitor tablets (Roche #11873580001) and/or PhosSTOP™ Phosphatase Inhibitor Tablets (Roche # 04906845001) ...
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bioRxiv - Molecular Biology 2024Quote: ... while remaining cells were harvested in 500 µL lysis buffer (50 mM Tris pH6.8, 2% SDS, 1x protease inhibitor (Roche Complete EDTA-free)) snap-frozen and stored at -70 °C until further processing.
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were prepared by adding 1 volume of SDS lysis buffer (60 mM Tris-HCl pH 7.5, 2% SDS, 10% glycerol) supplemented with 1x protease inhibitor cocktail (Roche Diagnostics, Cat # 11836153001) and 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Neuroscience 2023Quote: Whole brains or dentate gyrus regions (isolated from the brain slices) were lysed in 2% sodium dodecyl sulfonate (SDS) with protease and phosphatase inhibitors (Roche Applied Sciences) and manually homogenized ...
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bioRxiv - Bioengineering 2022Quote: ... coli CFPS of GRFT was conducted at a 25 μL scale in 2 ml Eppendorf tubes using the PANOx-SP system utilizing a phosphoenolpyruvate (Roche, Indianapolis, IN), amino acids (Sigma Aldrich ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... minced finely and digested in pre-warmed PBS (with calcium and magnesium) with 2 mg/ml of collagenase A (Roche, Basel, Switzerland) for 1h on a rotating wheel at 37°C ...
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bioRxiv - Physiology 2023Quote: ... autofluorescence was quenched by treating paraffin-embedded sections with PBS/BSA (5%) for 2 h before performing TUNEL staining (Roche, Basel, Switzerland) according to the manufacturer’s protocol and using Proteinase K treatment ...
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bioRxiv - Biochemistry 2024Quote: ... 1% 2-mercaptoethanol and 0.02% bromophenol blue) containing protease inhibitors (0469315900, cOmplete® Mini EDTA-free Protease Inhibitor Cocktail Tablets, Roche, Mannheim, Germany). The samples were separated by SDS-PAGE using 7.5% or 10% acrylamide gels at 100 V for about 2 hours ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µl of the samples’ cDNA was mixed with 5 µl of FastStart SYBR Green Master (ROX; Hoffmann-La Roche, Basel, Switzerland), 0.25 µl of each primer (final concentration 500 nM) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were carried out on 2 µL of cDNA using 7 µL of FastStart SYBR Green Master Mix (Roche, Basel, Switzerland), 2 µL of ddH2O (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were extracted into a Triton X-100 buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.5 mM EDTA, 1% Triton X-100, supplemented with 1× protease inhibitors - Roche). Extracts were incubated on ice for 30 min then clarified by centrifugation at 12,000g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed 2 x with PBS2+ and 4 ml lysis buffer (50 mM Tris-HCl, pH 7.4, 300 mM NaCl, 1% Triton X-100, 0.1% BSA, 1 x protease inhibitors [Roche]) was added to the washed samples ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was replaced with RIPA buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM MgCl2, 1% NP-40, 0.5% sodium deoxycholate, 1x complete protease inhibitor [Roche]) including 1% SDS ...
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bioRxiv - Developmental Biology 2021Quote: Cell pellets were incubated 20 minutes with RIPA lysis buffer (50 mM Tris-HCl pH7.5, 300 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 1X Roche COMPLETE Mini EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2020Quote: ... for Western blot analysis probing for HA and FLAG epitopes using 1° antibody = 1:500 mouse αHA (Roche), or 1:500 mouse αFLAG (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... previously washed three times with RIPA 0.1% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Developmental Biology 2019Quote: ... 1.5% Triton X-100, 1% IGEPAL, 0.5% Sodium Deoxycholate, 0.1% SDS, 1 mM DTT, 1x Complete protease inhibitor [Roche]) for 30 minutes at 4°C under rotation ...
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bioRxiv - Neuroscience 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA and 1X protease inhibitor cocktail; Roche). Anti-FLAG M2 Magnetic Beads (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with RIPA buffer (25 mM Tris HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche]). The lysates were subjected to SDS-PAGE under denaturing conditions in 12% polyacrylamide gels using Bio-Rad Mini PROTEAN electrophoresis system (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Ground cell pellet was collected and resuspended end-over-end for 1 h in lysis buffer (160 mM HEPES pH 7.5, 400 mM NaCl, 0.25 mM PMSF, 1 Roche cOmplete™ Protease Inhibitor Cocktail tablet ...
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bioRxiv - Immunology 2022Quote: ... and then minced and dissociated in digestion buffer (RPMI containing collagenase (1 mg ml−1 collagenase D; Roche), DNase I (100 μg ml−1 ...
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bioRxiv - Neuroscience 2021Quote: ... containing Phosphatase (1 tablet per 3.3ml lysis buffer) and Protease (1 tablet per 16.67ml lysis buffer) inhibitor (Roche cOmplete tablets ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 viral load was determined using the COBAS® AmpliPrep/COBAS® TaqMan HIV-1 test (Roche). PBMCs were purified using BD Vacutainer® CPT™ Mononuclear cell preparation tubes (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... DNAse I (0.2 mg ml−1 dissolved in PBS) and Liberase (0.65 Wuensch units ml−1, both Roche, dissolved in HBSS supplemented with 8 % FCS) ...
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bioRxiv - Physiology 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
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bioRxiv - Genetics 2019Quote: ... The supernatant was removed and the pelleted cells lysed by 3-cycles of freezing/thawing in 150 μl lysis buffer (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 1 mM EDTA, 0.1% IGEPAL, 1 mM DTT, 1x Complete Protease Inhibitor [Roche]). Cell extracts were centrifuged for 5 min at 12000 rpm ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet was suspended in 1 mL of cold lysis buffer (50 mM TRIS-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail ...
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bioRxiv - Microbiology 2020Quote: ... 100 mM Tris-HCl pH 8.0, 5% glycerol, 1 mM DTT, 0.1% CHAPS, 1 μg/mL avidin, cOmplete, EDTA-free protease inhibitors [Roche]), rotated at 4°C for 30 min ...
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bioRxiv - Plant Biology 2019Quote: ... Soluble proteins were extracted using 50 μl of protein extraction buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Tween-20, 1 mM DTT and 1×Complete protease inhibitors [Roche]), incubated on ice for 5 min ...
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bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-c-Myc primary antibody solution (GenScript, 1: 2,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) overnight at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) for 1-2 hrs ...
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bioRxiv - Molecular Biology 2019Quote: ... 1% Triton X-100, 0.1% deoxycholate Na, 0.1% SDS, 1 mM AEBSF [Euromedex] and cOmplete EDTA-free [Roche]) with glass beads (diameter ...