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Citations for Roche :
3701 - 3750 of 5048 citations for Cow Melanocyte Stimulating Hormone Receptor MC1R ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... nasal and tonsil swab) to a final elution volume of 80ul using MagNA Pure LC RNA isolation kit (Roche) and the KingsFisher Flex 96 robot (Thermofisher) ...
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bioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Sequencing libraries were loaded on a NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Library preparation was carried out with 500 ng sheared DNA using Kapa Hyper library prep kit (Roche, Basel, Switzerland). Barcodes were added using the Illumina UD 96 index kit (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... The isotypes of anti-Drosophila antibodies were determined by the IsoStrip™ Antibody Isotyping Kit (11493027001 Roche, Basel, Switzerland) according to the instructions of the manufacturer.
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bioRxiv - Immunology 2021Quote: ... The PCR products were used to synthesize dsRNA using a T7 RNA Polymerase Kit (Sigma-Aldrich RPOLT7-RO ROCHE). Generated dsRNAs were treated with TURBO DNA-free Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of total RNA was reverse transcribed using a Transcriptor First Strand cDNA synthesis kit (Roche Applied Science) and the provided random hexamer primers for the mix preparation ...
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bioRxiv - Genomics 2020Quote: ... An Illumina compatible NGS library was then prepared with 50ng of genomic DNA using the KAPA HyperPlus kit (Roche). Sequencing was performed on a NextSeq500 instrument (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... Stranded RNA libraries were prepared with 100ng total RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche). RNA sequencing was performed on Illumina NovaSeq 6000 system (Illumina) ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from BHI broth cultures using the High Pure PCR template preparation kit (Roche, Potsdam, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... The library concentration was determined by qPCR using the Library Quantification Kit Illumina Platforms (Kapa Biosystems, Boston, MA, USA). An equimolar pool of the barcoded libraries was made and the pool was sequencing on a HiSeq 2500 system (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... stranded RNA libraries were prepared with 100ng total RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche). RNA sequencing was performed on Illumina NovaSeq 6000 system (Illumina).
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bioRxiv - Genomics 2020Quote: ... the total DNA was sheared to ~500 bp size fragments and processed using KAPA LTP Library Preparation Kit (Roche Sequencing Solutions ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were prepared from these diluted samples using a miniaturized version of the KAPA HyperPlus kit (Roche, KK8514) with Illumina-compatible KAPA Unique Dual-Indexed Adapters (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... antisense and sense digoxogenin (DIG)-labeled RNA probes were generated using the DIG RNA labeling kit (Roche Diagnostics, Germany), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using the Transcriptor First-Strand cDNA Synthesis Kit with anchored oligo (dT)18 primers (Roche; 04379012001). Gene expression levels were analyzed by real-time PCR on an Applied Biosystems model 7500 Software (version 2.0.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... The TUNEL method was used to quantify apoptosis following the manufacturer’s instructions (In Situ Cell Death Detection kit; Roche). The percentage of TUNEL-positive cells in each kidney was quantified as described above ...
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed following citrate buffer antigen retrieval using an in situ cell death detection kit (Roche, 11684795910). Positive control slide was treated with deoxyribonuclease to induce DNA damage (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... and 500 ng of DNA-free RNA was reverse transcribed using the First Strand cDNA Transcriptor Synthesis Kit (Roche Life Science ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels were determined by qRT-PCR with KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems, KK4610). cDNA was synthesized from human cells using SuperPrep II Cell Lysis & RT Kit (TOYOBO ...
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bioRxiv - Immunology 2022Quote: ... Apoptotic cells were detected in dissected guts using the TMR red In Situ Cell Death Detection Kit (Roche; 12156792910) following standard kit staining protocol ...
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bioRxiv - Genomics 2022Quote: A DNA library was prepared from the WGA sample using the Kapa LTP Library Preparation Kit (KAPA Biosystems, Roche7961880001) and sequenced on the Illumina MiSeq platform with the MiSeq Reagent Kit v3 to generate 2×150 bp paired-end reads ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were prepared by the Bauer Core Facility at Harvard University using a KAPA stranded RNA HyperPrep Kit (Roche Sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of purified RNA was subjected to reverse transcription using a Transcriptor First Strand cDNA Synthesis Kit (Roche) and 1 µl of RNase A/T1 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction were carried out using MagNA Pure LC total Nucleic acid isolation kit on the automated MagNA Pure LC2.0 platform (Roche) from two to five colonies picked up from fresh cultured plates.
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bioRxiv - Microbiology 2022Quote: ... Library preparation was performed using ∼ 1ug of total RNA processed using RiboErase kits to remove ribosomal RNA (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 °C for 8 min ...
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bioRxiv - Microbiology 2022Quote: Quantitative Real-time PCR assays were performed with the KAPA SYBR FAST qPCR Master Mix (2X) Kit (Kapa Biosystems) on a LightCycler 1.5 (Roche ...
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bioRxiv - Microbiology 2022Quote: ... the water phase was used for RNA isolation with High Pure RNA Isolation Kit (Roche Diagnostics GmbH, Mannheim, Germany), yielding >3 μg total RNA per sample ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction was carried out using the MagNA Pure 96 DNA and Viral NA Small Volume Kit (Roche, Diagnostics) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... were produced with DIG-UTP by in vitro transcription using the Sp6 and T7 promoters according to the manufacturer’s protocol (RNA-labeling kit; Roche). The sections were treated as follows ...
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bioRxiv - Neuroscience 2022Quote: ... Antisense and sense single-strand riboprobes were transcribed in vitro using DIG or FITC RNA labeling kit (Roche Diagnostics). cRNA probes for Gad155 and Slc17a756 were used.
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bioRxiv - Neuroscience 2022Quote: ... and the pool was quantified by qRT-PCR using the KAPA Library Quantification Kit - Illumina/Universal (KAPA Biosystems, KK4824) in combination with the Life Technologies Viia 7 real time PCR instrument ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR reactions were performed using the KAPA SYBR FAST qPCR Master Mix (2X) Kit (KAPA Biosystems, Wilmington, USA) on the QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... according to the manufacturer’s protocol and were transcribed into cDNA with the 2nd generation 5’/3’ RACE Kit (Roche) in combination with the Expand High Fidelity PCR System (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit; Roche Tissue Diagnostics). For haematoxylin and eosin (H&E ...
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bioRxiv - Physiology 2022Quote: Both ATP release and intracellular ATP from isolated mesenteric arteries were performed using a ATP Bioluminescence HSII kit (Roche) kit as previously described.3
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bioRxiv - Molecular Biology 2022Quote: The psyllid midguts were diagnosed for cell-death or apoptosis using In situ cell-detection kit TMR-red (Roche) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... six reverse primers (10 μM) and Robust DNA polymerase from the KAPA2G Robust PCR Kit (Kapa Biosystems, Wilmington, MA). Cycling conditions were ...
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bioRxiv - Plant Biology 2024Quote: ... and the library was quantified by qPCR against a standard curve with the KAPA Library Quantification Kit (Kapa Biosystems). Libraries were sequenced on a NovaSeq6000 Illumina platform in 150PE mode ...
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bioRxiv - Plant Biology 2024Quote: ... 1 μg of the PCR product was used for RNA probe labeling with T7 RNA polymerase as per the DIG Northern Starter Kit’s Instruction Manual (Roche). Post-hybridization washes and immuno-chemiluminescent detection of the bound probe were conducted following the instructions provided in the DIG Northern Starter Kit manual (Roche) ...
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bioRxiv - Plant Biology 2024Quote: We fragmented 700 ng of high-molecular-weight DNA using a Covaris S220 sonicator and a WGS library was generated for both species using the KAPA Hyper Prep kit with a PCR-free protocol according to the manufacturer’s instructions (Roche). We applied final size selection using a 0.7-fold ratio of AMPureXP beads ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μm sections were stained according to instruction of In situ Cell Death Detection Kit (Roche, Basel, Switzerland, #11684817910). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 1 µg of total RNA using the KAPA Stranded mRNA Hyperprep Kit (96rxn) (Roche, KK8581) according to manufacturer’s specifications ...
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bioRxiv - Genetics 2023Quote: ... Sequencing libraries were prepared using 100 ng fragmented DNA with the KAPA HyperPrep Library Prep Kit (Roche, Indianapolis, IN) and NEXTFLEX UDI barcodes (PerkinElmer ...
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bioRxiv - Molecular Biology 2024Quote: ... The preparation of next generation sequencing libraries was done using the KAPA HyperPrep ChIP Library kit (Roche Sequencing solutions) at the molecular biology and functional genomics platform of the Institut de Recherche Clinique de Montréal (IRCM) ...
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bioRxiv - Molecular Biology 2024Quote: ... membrane hybridization and detection were performed using DIG-High Prime DNA Labelling and Detection Starter Kit II (Roche, #11585614910) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Molecular Biology 2024Quote: Analysis of ChIP-purified DNA by quantitative PCR (qPCR) was performed using KAPA SYBR Fast qPCR kit (KAPA Biosystems) and StepOnePlus real-time PCR systems (Life Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche, #11175025910). Probes were then cleaned with MegaClear Kit (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... For library preparation the Kapa LTP Library Preparation Kit for Illumina Platforms was used (Kapa Biosystems, Part Number KK8232). The cDNA was filtered into the End Repair step of the method and the library preparation was followed as per the protocol through to Adapter Ligation ...