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Citations for Roche :
3701 - 3750 of 8781 citations for 6H Imidazo 4 5 e 2 1 3 benzothiadiazole 7 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: The body weight of all mice was recorded weekly and blood glucose (BG) levels were measured every 5 weeks after fasting for 12 h using a glucose analyzer (Accu-Chek; Roche, Rotkreuz Switzerland) over the tail vein ...
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bioRxiv - Cancer Biology 2021Quote: We established single-cell suspensions of surgically resected tumors by taking a small piece of PDAC tumor tissue collected in a cell-dissociation media [5 ml of minimal essential media (MEM) containing 100μl of Liberase-TM (2.5 mg/ml stock solution Roche/Sigma Aldrich Cat# 5401046001), 50μl of Kolliphor®P 188 (15 mM stock solution Sigma Cat# K4894) ...
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bioRxiv - Cancer Biology 2022Quote: For antibody incubation 5 µl of the DigiWest Bead mixes were added to 50 µl assay buffer (Blocking Reagent for ELISA (Roche, Rotkreuz, Switzerland) supplemented with 0.2% milk powder ...
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SARS-CoV-2 Point Mutation and Deletion Spectra, and Their Association with Different Disease OutcomebioRxiv - Microbiology 2022Quote: ... Each region was amplified from 5 μl of the RNA preparation by RT-PCR using Transcriptor One Step RT-PCR kit (Roche Applied Science). To perform the RT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... A master mix was made for each qRT-PCR target containing 5 ul of KAPA Universal SYBR Fast PCR mix (KAPA Biosystems, #KK4602), 0.6ul of 5 uM forward primer ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was diluted to a final concentration of 5 ng/μl and RT–qPCR was performed with KAPA SYBR® FAST qPCR kits (KAPA Biosystems) on a C1000 thermal cycler ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-sense riboprobes against target genes were synthesized from 5 µg linearized plasmids using digoxigenin-(DIG) or fluorescein-labeled uridylyltransferase (UTP) (#11685619910, #11277073910, Roche, Mannheim, Germany) and the MAXIscript in vitro Transcription Kit (#AM1312 ...
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bioRxiv - Pathology 2021Quote: ... 2 x 106 insect cells from 48 post infection Hi5 cells incubated at 21°C were resuspended in lysis buffer (20 mM Na phosphate pH 7.5, 500 mM NaCl, 5% glycerol, Complete protease inhibitor, Roche Diagnostics, Rotkreuz, Switzerland) and sonicated until cells were > 99% lysed as determined by microscopic examination ...
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bioRxiv - Neuroscience 2019Quote: ... samples were pretreated with hybridization buffer (50% formaldehyde, 5 × SSC, 0.1% tween, 100 ug/ml tRNA [Roche], 50 ug/ml heparin [Sigma]) for 2 hr at 65°C ...
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bioRxiv - Physiology 2020Quote: ... Tissues were homogenised in assay buffer provided in the kit with the addition of 5 μL of protease inhibitor cocktail (Roche, Basel Switzerland) and centrifuged at 800 × g for 10 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... 0.1% NP-40 and 5 mM β-mercaptoethanol) per gram of cells in the presence of protease inhibitors (Complete™ EDTA-free Protease Inhibitor Cocktail, Roche). Suspensions were flash frozen in droplets and cells subjected to cryogenic grinding using a Ball Mill MM 400 (5 cycles of 3 minutes at 20 Hz) ...
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bioRxiv - Biochemistry 2021Quote: The pupae infected by the baculovirus were homogenized with a buffer solution (20 mM Tris-HCl, 150 mM NaCl, 5 mM DTT, 1mg/ml phenylthiourea, one inhibitor cocktail tablet (EASYpack, Roche, Basel, Switterland) per 50 ml solution ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 5 mM imidazole, 0.4% Triton X-100, supplemented with Roche cOmplete Protease Inhibitor Cocktail) and lysed by sonication ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in lysis buffer (20 mM HEPES pH 8.0, 150 mM NaCl, 5 mM imidazole, 0.1% Tween 20, supplemented with Roche cOmplete Protease Inhibitor Cocktail) and lysed by sonication ...
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bioRxiv - Cancer Biology 2020Quote: ... to amplify the ligation product with 5 PCR cycles using 2x KAPA-HiFi HS Ready Mix and 10X KAPA primer mix (Roche Kapa Biosystems). The libraries were sequenced on HiSeq 4000 or NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... Femurs were then decalcified and paraffin sections (5-μm thickness) were stained with hematoxylin and eosin or reticulin (Reticulum II Staining Kit, Roche, Tucson, AZ) to assess fibrosis ...
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bioRxiv - Immunology 2021Quote: ... it was added 50 µL of fresh TUNEL reaction mixture composed of 5 µL of enzyme solution mixed with 45 µL of labeling solution (In Situ Cell Death Detection kit, POD, ROCHE, version 15.0) for 1 hour at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Antisense riboprobes against target genes were synthesized from 5 μg linearized plasmids using digoxigenin-(DIG) or fluorescein-labeled uridylyltransferase (UTP) (#11685619910, #11277073910, Roche, Mannheim, Germany) and the MAXIscript in vitro Transcription Kit (#AM1312 ...
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bioRxiv - Neuroscience 2023Quote: ... 10-15 fly heads were homogenised in guanidine extraction buffer (5M Guanidinium-HClSigma G3272, 50 mM Hepes Sigma H3375, 5 mM EDTA, protease inhibitor cocktail Roche Complete Mini). The plate was coated with first antibody solution (Clone 6E10 ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were made using HEK-293/17 cells that were transfected with 5 μg of viral DNA using X-tremeGENE HP (Roche, Basel, Switzerland) after 48 h incubation ...
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bioRxiv - Cell Biology 2023Quote: The cell pellets were washed once in PBS and resuspended in 5 ml PBS containing protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Cell Biology 2023Quote: ... The microinjection was performed with glass capillaries (Eppendrof Femtotips, Cat# 930000035) loaded with 5 mg/mL of RNase A (Roche, Cat# 10109142001) mixed with Dextran-TAMRA in PBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Crosslinked cell pellets were lysed in 5 mL cold lysis buffer (10 mM Tris-HCl, pH7.5; 10 mM NaCl; 5 mM MgCl; 0.1 mM EGTA; 1x Roche Complete protease inhibitor cocktail). Lysis was allowed to proceed for minutes on ice with intermittent gentle pipetting to obtain homogeneous suspensions of nuclei ...
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bioRxiv - Molecular Biology 2024Quote: ... Alkaline phosphatase staining was conducted on primary OP6 cell cultures on Day-5 post-transfection using NBT/BCIP stock solution (Roche/Sigma 11681451001) or BCIP/NBT-Blue Liquid Substrate Systems for Membranes (Sigma B3804 ...
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bioRxiv - Neuroscience 2021Quote: ... and alkaline phosphatase-conjugated anti-DIG antibody (diluted 1:1,000 in DIG-1, Roche Diagnostics) for 2 h and washed twice with DIG-1 for 5 min each ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 hr at RT and incubated with anti-DIG-POD (1:500, 11207733910, Roche) and rabbit anti-RFP (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... Then WST-1 assay was performed by adding WST-1 reagent (cell proliferation reagent, Roche) directly to the culture wells ...
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bioRxiv - Neuroscience 2019Quote: ... 1% NP-40 and 1 mM dithiothreitol supplemented with protease (Roche, Welwyn Garden City, UK) and phosphatase (ThermoFisher ...
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bioRxiv - Neuroscience 2019Quote: ... the sections were subjected to blocking solution (1% Bovine Serum Albumin (Roche, 9048-49-1), 5% Normal Goat Serum (Thermoscientific ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% SDS) including 1 mM PMSF and 1×protease inhibitors (cOmpleteTM protease inhibitor cocktail, Roche) and stored at -20°C in 1 Laemmli buffer until usage ...
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bioRxiv - Cell Biology 2022Quote: EV preparations were lysed using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche) and EV concentration was determined using Pierce™ MicroBCA protein assay kit (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... After blocking for 1-2h with the 1% blocking reagent (Roche Applied Science, cat# 10057177103), sections were incubated with alkaline phosphatase-conjugated anti-DIG antibody (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were immunolabeled with anti-bromodeoxyuridine (BrdU) (Roche 11170376001; 1:250 to 1:500 dilution) to visualize BrU signal.
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bioRxiv - Immunology 2020Quote: ... at 1:1,000 dilution in 1% BSA in PBS and One-step ABTS substrate (Roche). Non-PfCSP reactive antibody ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM NA3VO4 and 1 µg/ml leupeptin supplemented with complete mini protease inhibitor (Roche). Denatured proteins (20-40 µg ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM MgSO4) supplemented with 1 mg/ml lysozyme and cOmplete Protease Inhibitor Cocktail (Roche), and subsequently lysed using sonication ...
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bioRxiv - Cell Biology 2019Quote: ... sections were incubated overnight with 1:1000 anti-digoxigenin-alkaline phosphate (11093274910 Roche, 1:1000) at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM MgSO4) supplemented with 1 mg/ml lysozyme and cOmplete Protease Inhibitor Cocktail (Roche). Cells were lysed by sonication ...
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bioRxiv - Genomics 2021Quote: ... pronase (0.4 mg mL-1) and DNase I (0.1 mg mL-1, Roche, Cat#10104159001), and then incubated for digestion at 37 °C in a water bath for 20 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% Triton and Complete® EDTA-free protease inhibitor cocktail (1 tablet/50 ml; Roche)) ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated in a 1:1000 dilution of 1 mg/ml DAPI (Roche Diagnostics) in PBS for 5 min followed by three washes with PBS ...
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bioRxiv - Biochemistry 2022Quote: Proteins were extracted with 1× RIPA buffer containing 1× complete-mini protease inhibitor (Roche: 11836170001) and 1× phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Genetics 2023Quote: ... 1% Triton X-100 and 1× cOmplete™ ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor (Roche). After removal of cell debris by centrifugation (twice 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... 1mM MgCl2 and 1%NP-40) supplemented with a protease inhibitor cocktail (Roche, 1:10000). Cell lysates were centrifuged at 10,000xg for 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... supplemented with 1 tablet/50 ml cOmplete proteinase inhibitor and 1 mM Pefabloc SC (Roche)) supplemented with 1 % formaldehyde ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.4) with 1 mM TCEP and 1 tablet complete EDTA-free protease inhibitor (Roche). The lysate was cleared by centrifugation at 30,000 g for 30 min at 4 °C and subsequently filtered through a 0.45 μm filter before incubation with 2.5 ml glutathione-conjugated sepharose beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... with protease inhibitor (1 mM phenylmethanesulfonyl fluoride and 1 x Complete Protease Inhibitor Cocktail; Roche), then sonicated with a Bioruptor (Diagenode) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM DTT) containing protease inhibitors (1 mM PMSF, 1x Roche complete protease inhibitor mix). The cells were lysed on ice for 10 min ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... 1 mM EGTA and 1 mM EDTA) supplemented with cOmplete™ protease inhibitors (Roche, #4693116001) and PhosSTOP™ phosphatase inhibitors (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM EDTA) supplemented with 1 mM DTT and protease and phosphatase inhibitors (Roche). Protein concentrations were measured with the Bradford assay (Bio-Rad) ...