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Citations for Roche :
3651 - 3700 of 10000+ citations for Rat Insulin Like Growth Factor 1 Receptor IGF1R ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... 100mM KCl, 10mM MgCl2, 1 mM PMSF, 5 mM Na3VO4, 5 mM NaF, 1 mM TCEP, cOmplete Protease Inhibitor Cocktail, Roche) in 2 steps ...
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bioRxiv - Neuroscience 2022Quote: ... brains were incubated with primary antibodies (1:200, chicken anti-GFP, Abeam, Cat# ab13970; 1:200, mouse anti-GFP, Roche, Cat# AB_390913 ...
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bioRxiv - Neuroscience 2022Quote: HEK293T cells transfected with N-terminally sfGFP-tagged indicated variants of TAOK1 were lysed with HKT buffer (25mM HEPES pH7.2, 150mM KCl, 1% Triton X-100, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, cOmplete). Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed in Western Blot (WB) lysis buffer (1% SDS, 10 mM Tris, 1 mM EDTA, phosphatase and protease inhibitors (Roche, 04906837001 and 04693159001 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated overnight at 4 °C with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:2500-1:4000, Roche). To visualize the RNA-probe binding ...
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bioRxiv - Plant Biology 2024Quote: The pulverized tissue was incubated in 30 mL of buffer 1 (400 mM sucrose, 10 mM Tris-HCl pH 8.0, 10 mM MgCl2, 5 mM β-mercaptoethanol, 1 mM PMSF, 1× Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
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bioRxiv - Genomics 2023Quote: ... resuspended in 850 μL RIPA buffer (1×PBS, 1% IGEPAL, 0.5% Sodium Deoxy-cholate, 0.1% SDS, Roche Protease Inhibitor Cocktail) and added to the beads ...
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bioRxiv - Molecular Biology 2023Quote: ... treated with 1 µL RNase A (Thermo) for 30 minutes followed by 1 µL Proteinase K (20 mg/mL, Roche) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 8, 450 mM NaCl, 1% CHAPS, 20 mM MgCl2, 5 mM ATP, 1 mM Dithiothreitol [DTT], 1X Roche Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μL 1 M spermidine, 500 µL 10% Triton X-100, and 10 ml glycerol in 38 ml dH2O, 1 Roche Complete Protease Inhibitor EDTA-Free (Millipore Sigma 11873580001)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysis buffer (150 mM NaCl, 1 mM EDTA, 50 mM Tris-HCl, 1% NP-40 with proteases and phosphatases inhibitors (Roche)) was added directly to the wells and cells were scraped and collected in pre-cooled Eppendorf tubes followed by sonification for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were washed three times with TBST and then incubated with appropriate secondary antibodies (2 µg/mL in TBST / 1% bovine serum albumin (BSA)) and 1 µg/mL DAPI (Roche) for one hour at room temperature ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... 1% Triton X-100, 2 mM EDTA, 2 mM EGTA, 1 mM PMSF, and complete protease inhibitor cocktail tablet; Roche), incubated with continuous shaking at 4°C for 20 min and then centrifuged at 12 000xg at 4°C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed in 1% NP-40 lysis buffer (1% NP-40, 50 nM Tris HCl, 150 mM NaCl) supplemented with 1 × EDTA-free protease inhibitor cocktail (Roche) and Benzonase (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking solution was removed and replaced with antibody solution containing 1% heat inactivated goat serum and a 1:5000 dilution of anti-DIG-AP antibody (Roche). Slides were then incubated overnight at 4°C in a humidified chamber ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26 ...
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bioRxiv - Microbiology 2022Quote: ... The total qPCR reaction volume was 1.5 □μL with 0.5□ μl of DNA (40□ng□μl-1) and 1□μL of LightCycler 480 SYBR Green I Master mix (Roche) containing 0.5□μM of PCR primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody incubation was performed for 16 h at 4°C in AB buffer supplemented with 1% v/v Fetal Calf Serum and anti-DIG antibody coupled to alkaline phosphatase (1∶5000 dilution; Roche). Sections were then washed thoroughly in AB and equilibrated in alkaline phosphatase buffer (AP - 0.1 M Tris–HCl pH ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with glass beads in 250 µl RIPA buffer (25mM Tris/HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Roche complete protease inhibitor cocktail; Roche PhosStop tablet) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM CDTA pH 8.0, 0.5% Triton-X-100 supplemented with 1 mM ATP, 1 mM PMSF, and Roche protease inhibitors) for 75 mins on a nutating rocker ...
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bioRxiv - Microbiology 2023Quote: ... 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF, 1X Roche protease inhibitor, 1X Roche phosphatase inhibitor) followed by a 30’incubation on ice ...
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bioRxiv - Neuroscience 2023Quote: Cell pellets were resuspended in a lysis solution composed by 1 X Phosphate Saline Solution (PBS) supplemented with 1 X Complete Protease Inhibitor Cocktail (Roche) and then sonicated with a BioRuptor UCD-200 (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with protein lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 5 mM EDTA, 1 mM Mg2Cl, 1% NP40 and supplemented with protease inhibitor cocktail, Roche), incubated on ice for 20 minutes and spun at 10,000 g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed with protein lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 5 mM EDTA, 1 mM Mg2Cl, 1% NP40 and supplemented with protease inhibitor cocktail, Roche), incubated on ice for 20 minutes and spun at 10,000 g for 10 min at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... 1% [v/v] Triton X-100, 150 mM NaCl, 1 mM EDTA, 10% [v/v] glycerol, and 1× protease inhibitor cocktail [Roche]) by rotating at 4 °C for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellets were washed once then resuspended in a 1:1 ratio with CHAPS lysis buffer + protease inhibitors (Roche Complete) and frozen dropwise in liquid nitrogen before freezer-mill lysis ...
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bioRxiv - Plant Biology 2024Quote: ... 100 mM NaCl, 80 mM KCl, 1% glycerol, 0.1 % Triton, 10 mM DTT, plus 1 mini protease inhibitor cocktail tablet, Roche, Switzerland) per 20 ml of extraction buffer ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 x phosphatase inhibitor cocktail (PhosSTOP, Roche). Total protein concentration was determined with Bradford protein assay kit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 μg ml−1 of DNase (Roche, #10104159001), 50 μg ml−1 of RNase (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... Mouse monoclonal to GFP (Roche #11814460001, 1:2000), Rabbit monoclonal to LRRK2 phospho-S1292 (Abcam #ab203181 ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM PMSF and protease inhibitor cocktail (Roche). Cells were scrapped and passed through a syringe with needle gauge 26 several times avoiding foam ...
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bioRxiv - Cell Biology 2020Quote: ... rhodamine α-DIG Fab fragments (Roche 1:20) followed by Texas Red α-sheep antibody (Vector Labs ...
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bioRxiv - Cell Biology 2019Quote: ... 1% Triton-X100 and protease inhibitors (Roche Diagnostics). of clarified lysate was obtained by centrifugation at 16,000xg 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-GFP (1:200 dilution; Roche, 11814460001) was used for staining Cse4-GFP and rat anti-HA (1:200 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were blocked with 1% blocking reagent (Roche) for 1 h at RT ...
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bioRxiv - Genetics 2020Quote: ... 1 mM EDTA and proteinase inhibitor (cOmplete, Roche) for 30 min at 4 °C on a rocking platform followed by centrifugation at 15,000 rpm for 15 min at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mg/ml yeast tRNA (Cat. # 10109509001, Roche), 1×Denhardt’s [1% (w/v ...
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bioRxiv - Developmental Biology 2020Quote: ... 1× complete protease inhibitor cocktail (Roche, Lot # 3024150)] ...
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bioRxiv - Biophysics 2022Quote: ... 1 cOmplete EDTA free protease inhibitor tablet (Roche) per 50 ml Buffer L ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with collagenase (Roche, 11088793001, 1 mg/mL) and hyaluronidase (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mM EGTA and protease inhibitors (Roche Diagnostics). For homogenization ...
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bioRxiv - Molecular Biology 2020Quote: ... GFP 1:1000 dilution (Roche 11814460001 Lot# 27575600). After three five-min washes with gentle rotation in blocking buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 × cOmplete EDTA-free protease inhibitor cocktail (Roche) and 0.5 U/µl RNasin (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1×EDTA-free protease inhibitor cocktail (Roche) (Guo et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 10% glycerol) containing protease inhibitors (1:100, Roche) for 45 min on a rotor at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Dispase II (Roche, 1 mg/ml, Cat. No.SCM133) and Dnase I (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... in the presence of 1 mM dATP (Roche) and 40 U of RNAseOUT (Thermo Scientific ...