Labshake search
Citations for Roche :
3601 - 3650 of 6468 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... The samples were then blocked in blocking solution (maleic acid buffer containing 2% Boehringher-Mannheim blocking reagent) and incubated overnight at 4°C in blocking solution containing anti digoxigenin antibody (Roche) diluted at 1:2000 ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated overnight at 4 °C with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:2500-1:4000, Roche). To visualize the RNA-probe binding ...
-
bioRxiv - Molecular Biology 2024Quote: ... 15 µl of IPed proteins and 10 µl of WCE were submitted to SDS-PAGE and analyzed by immunoblotting using anti-HA antibody (1:250, ref.12013819001, Roche), anti-Flag antibody (1:500 ...
-
bioRxiv - Neuroscience 2024Quote: ... DIG-labeled hybrids were detected with an anti-DIG antibody from sheep conjugated with alkaline phosphatase as recommended by the manufacturer (Roche) by incubating the slice overnight at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were permeabilised by 10 minutes incubation with 0.1% Triton X-100 on ice and incubated with blocking buffer (10% FBS in 1X PBS) for 90 minutes and then probed with the primary antibody (anti-HA rat 1:100 (Roche) for 2 hours on rotor and after three washing with 1x PBS cells were probed with anti-rat Alex Flour-488 (1:500 ...
-
bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
-
bioRxiv - Developmental Biology 2024Quote: ... blocked in MAB-Buffer2 (20% sheep serum, MAB-Buffer1) for 1h and incubated with anti-DIG-AP antibody (1:4000, Roche) in MAB-Buffer2 overnight at 4 °C.
-
bioRxiv - Microbiology 2024Quote: ... BSA in TBST (TBSTB) and probed with rat monoclonal Anti-HAHA High Affinity antibody (clone 3F10, cat. no. 11867423001, Roche) diluted 1:1000 in TBSTB for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... Blots were washed with high stringency (0.5× SSC, 70°C) and developed with alkaline phosphatase-conjugated anti-DIG antibody (Roche, Switzerland) with CDP-Star substrate in accordance with the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... HA-tagged pUL97 kinase was immuno-precipitated from transfected HEK 293T cells using the rat anti-HA antibody clone 3F10 (Roche). Kinase inhibitors were added to the kinase reactions at final concentrations of 1 μM (CDKIs ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... In situ hybridizations were done according to a standard protocol (DIG mix, DIG antibody and BM purple were purchased from ROCHE). Photographs were taken on a Leica M205FA stereomicroscope with a Leica DFC450 digital camera (Wetzlar ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Slides were incubated with 50 μL of block buffer containing a fluorescein-labeled anti-DIG antibody (sheep, 1:100, Roche) for 60 min at 37°C ...
-
bioRxiv - Genetics 2023Quote: ... Phosphorylation of Sch9 was monitored by western blotting probed with anti-HA 3F10 antibody (1:2000; Roche Life Science, USA) followed by goat anti-rat HRP-conjugated antibody (1:5000 ...
-
bioRxiv - Developmental Biology 2023Quote: ... sections were washed three times with TBST and then incubated with appropriate secondary antibodies (2 µg/mL in TBST / 1% bovine serum albumin (BSA)) and 1 µg/mL DAPI (Roche) for one hour at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... The total contents of Tie1-HA and Tie1KD-HA were assayed in a parallel run using anti-HA antibody (1:2000, Roche). As a loading control ...
-
bioRxiv - Molecular Biology 2023Quote: ... These DNA molecules were then flow-stretched so that the dig-ends were anchored at chromium pedestals coated with anti-dig antibody (Roche). A pre-defined barrier-pedestal distance of 12 µm and orthogonal attachment chemistry yielded uniform and unambiguous orientation of the double-tethered dsDNA curtain ...
-
bioRxiv - Molecular Biology 2023Quote: ... These DNA molecules were then aligned as the chromium barriers and flow-stretched so that the dig-tagged ends were anchored to chromium pedestals coated with anti-dig antibody (Roche). These DNA molecules were then flow-stretched so that the dig-ends were anchored at chromium pedestals coated with anti-dig antibody (Roche) ...
-
bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26 ...
-
bioRxiv - Developmental Biology 2023Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1/4000 in the blocking solution ...
-
bioRxiv - Neuroscience 2023Quote: ... and then incubated in an anti-digoxigenin antibody (alkaline-phosphatase-coupled anti-digoxigenin; RRID:AB_514497, diluted 1:3,500; Roche Diagnostics; Table 2) for 30 minutes at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... The primary antibodies and respective concentrations used in this study are the following: 4’,6-diamidino-2-phenylindole (DAPI) (1:500, Roche), anti-Ki67 (1:100 ...
-
bioRxiv - Genetics 2023Quote: ... for 30 min at room temperature and incubated for 2 h at room temperature or overnight at 4°C with primary antibodies against GFP (Roche), androgen receptor (Invitrogen) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Hybridization was carried out at 600C overnight and the slides were washed and probed with an alkaline phosphatase-conjugated anti-DIG antibody (Roche) at 1:2000 dilution ...
-
bioRxiv - Molecular Biology 2023Quote: ... Antibody incubation was performed for 16 h at 4°C in AB buffer supplemented with 1% v/v Fetal Calf Serum and anti-DIG antibody coupled to alkaline phosphatase (1∶5000 dilution; Roche). Sections were then washed thoroughly in AB and equilibrated in alkaline phosphatase buffer (AP - 0.1 M Tris–HCl pH ...
-
bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed and incubated 1 hs at RT with goat anti-mouse secondary antibody conjugated to HRP (Roche) at 1/20000 dilution in PBST - 5% milk.
-
bioRxiv - Neuroscience 2023Quote: ... Washes were followed by blocking in 10% heat-inactivated sheep serum for 1-3 hours and incubation in buffer containing sheep antidigoxigenin antibody (Roche) at 1:5000 dilution for 16-20 hours at 4oC ...
-
bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 4°C overnight with the primary antibody mouse IgG1 Anti-Tbx6 (home-made, clone A83, dilution 1:500) in 2% Blocking Reagent (Roche) and 5% sheep serum (Sigma ...
-
bioRxiv - Pathology 2023Quote: ... The preparations were washed with buffer 1 and incubated for 2h at 37°C with an anti-digoxigenin antibody conjugated to alkaline phosphatase (1:200 in blocking solution; 11093274910, Roche). Next ...
-
bioRxiv - Plant Biology 2023Quote: ... membranes were blocked with 5% skim milk and then incubated in the primary anti-GFP mouse monoclonal antibody (Roche, 11814460001), followed by goat anti-mouse HRP conjugate (BioRad ...
-
bioRxiv - Physiology 2023Quote: ... co-staining for Cluster of Differentiation 15 (CD15) antigen was carried out using the CONFIRM Anti-CD15 Mouse Monoclonal Primary Antibody (Ventana/Roche; Roche Diagnostics ...
-
bioRxiv - Physiology 2023Quote: ... co-staining for Cluster of Differentiation 15 (CD15) antigen was carried out using the CONFIRM Anti-CD15 Mouse Monoclonal Primary Antibody (Ventana/Roche; Roche Diagnostics ...
-
bioRxiv - Plant Biology 2024Quote: ... The immuno-complexes was then analysed on a 10% SDS-PAGE gel using immunoblotting methods with anti-HA antibodies (Anti-HA High Affinity Roche 3F/10 ...
-
bioRxiv - Plant Biology 2024Quote: ... and the immuno-complexes was then analysed on a 10% SDS-PAGE gel using immunoblotting methods with Abcam (Cambridge, UK) anti-GFP and anti-HA antibodies (Anti-HA High Affinity Roche 3F/10 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using an anti-rabbit HQ and anti-HQ HRP detection system (Roche, catalog 7017812001 and 7017936001) followed by Chromomap DAB IHC detection (Roche ...
-
bioRxiv - Neuroscience 2024Quote: ... Slides were then incubated overnight at 4°C in 0.1% goat serum with 0.05% alkaline phosphatase-conjugated DIG antibodies (Roche Cat # 11093274910) and washed before development was carried out using detection buffer containing 20 μL/mL of NBT/BCIP ...
-
bioRxiv - Neuroscience 2024Quote: ... life-Tein LLC) was mixed with 50µl of PBS containing 1% Tween20 and 5ng/mL anti-HA-Peroxidase 3F10 antibody (Roche #12013819001) and transferred to the wells ...
-
bioRxiv - Molecular Biology 2024Quote: ... 500 μL of whole cell extract (WCE) was incubated overnight at 4°C with 2 μg anti-c-myc antibodies (mouse monoclonal, clone 9E10, Roche) for immunoprecipitation of myc-tagged proteins ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR products were purified using the High Pure PCR product Purification Kit (Roche, Denmark). Subsequently the purified PCR products were sent for sequencing at LGC Genomics (Berlin ...
-
bioRxiv - Microbiology 2020Quote: ... EMSAs were performed on cell extracts using a DIG Gel Shift Kit (2nd generation) (Roche). DNA fragments were labeled at their 3’ end with digoxigenin-11-ddUTP using terminal transferase ...
-
bioRxiv - Plant Biology 2019Quote: Quantitative reverse transcription PCR was performed using KAPA SYBR FAST ABI PRISM kit (Kapa Biosystems) using a QuantStudio™ 12K Flex Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... no degradation peaks) were quantified using the Library Quantification Kit from Illiumina (Kapa Biosystems, KK4603). Libraries were pooled to a final concentration of 10nM and sequenced single-end using the Illumina HiSeq 4000.
-
bioRxiv - Cancer Biology 2021Quote: ... RNAseq libraries were prepared from 300ng of total RNA using the Kapa Hyperprep kit (Roche) with 14 cycles of PCR ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared using the commercially available KAPA Stranded mRNA-Seq Kit (Roche). In brief ...
-
bioRxiv - Cancer Biology 2021Quote: ... An exome capture was then applied using the Nimblegen SeqCap EZ Exome V2 kit (Roche), and both normal/tumor matched samples were sequenced paired-end at 100 bp on the Illumina HiSeq4000.
-
bioRxiv - Cancer Biology 2021Quote: ... cell death was measured by determining LDH release using the Cytotoxicity Detection Kit (LDH; Roche).
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised according to instructions for the Transcriptor first strand cDNA synthesis kit (Roche). Lyophilised primers (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2020Quote: Cell stress was assessed by LDH release using the Roche Cytotoxicity Detection Kit (Roche 11644793001) in cells treated with OxPC (0-160 μg/mL ...
-
bioRxiv - Cell Biology 2020Quote: Apoptosis and Senescence was detected using the In Situ Cell Death Detection Kit (Roche, SW) according to manufacturer’s instructions combined with Immunofluorescence against the p15/16 senescence-associated factor ...
-
bioRxiv - Cell Biology 2020Quote: ... Libraries were then quantified using the Kapa quantification Complete kit for Illumina (Kapa Biosystems, KK4824) on an Applied Biosystems 7500 Real Time qPCR system ...
-
bioRxiv - Developmental Biology 2021Quote: ... Washing of captured material was done using Nimblegen SeqCap EZ hybridisation and wash kit (Roche) and captured sequences were isolated using M-270 Streptavidin Dynabeads (Invitrogen) ...