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Citations for Roche :
301 - 350 of 570 citations for Sulfo NHS LC Biotin sodium since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... containing 1% NP-40 and 0.25% sodium deoxycholate plus a complete protease inhibitor cocktail (Roche) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... containing 150 mM sodium chloride and 1 mL of 50 x Complete Proteinase inhibitor (Roche 45582400 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% NP-40 and 0.5% sodium deoxycholate) with protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany) and kept on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.1% (w/v) sodium dodecyl sulfate) for lysis containing complete protease inhibitor cocktail (Roche). Lysates were homogenized using a handheld cell disruptor and sample concentrations were determined using a DC Lowry protein assay (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% sodium dodecyl sulfate) with added protease and phosphatase inhibitors (Roche Applied Science, Mannheim, GER). Myofibrillar proteins were pelleted by centrifugation at 700g for 5min and the protein concentration of the sarcoplasmic fraction (supernatant ...
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bioRxiv - Cancer Biology 2022Quote: ... 10% Glycerol) containing 1 mM Sodium Vanadate and the protease inhibitor cocktail Complete Mini (Roche). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% SDS and 0.5% sodium deoxycholate) supplemented with EDTA-free protease inhibitor (Roche Applied Science) and PhosSTOP phosphatase inhibitor (Roche Applied Science) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% SDS) supplemented with 1 mM Sodium orthovanadate (Na3VO4) and protease inhibitors (Roche, Cat. # 11836170001). Total protein concentration was measured by Bradford protein assay (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... 0.5% w/v sodium deoxycholate) with a 1x protease inhibitor cocktail (Roche, VWR, Cat# PI88266) and 0.5% v/v SUPERase In (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate) supplemented with 2% v/v protease inhibitor cocktail (Roche; Catalogue #11697498001) and 1% v/v phosphatase inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1% sodium dodecyl sulfate (SDS) and 1% TritonX-100) supplemented with Protease Inhibitor (Roche, 11873580001). Extracted proteins were boiled for 10 min with 2X Laemmeli buffer (100mM Tris-HCl pH6.8 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 mM sodium orthovanadate and Complete mini EDTA free protease inhibitor cocktail (Roche Diagnostics). Soluble proteins were separated by SDS-PAGE ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5% sodium deoxycholate) supplemented with 1 tablet per 10ml buffer of the protease inhibitors (Roche) and 4U per ml buffer Turbo-DNase (Thermofisher) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate) supplemented with protease and phosphatase inhibitor cocktails (Roche, 04693159001 and 04906837001). For probing endogenous ITGβ5 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.1% w/v sodium dodecyl sulfate) with cOmplete protease inhibitor cocktail (Roche, Basel, Switzerland). The EV and cell lysate protein concentrations were determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% Sodium Deoxycholate) complemented with protease inhibitor cocktail tablet (Roche, Ref 05 892 791 001), PMSF (1mM final) ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.5% sodium deoxycholate) supplemented with a complete protease inhibitor cocktail (#11836153001, Roche, Basel, Switzerland). The cell lysate was centrifuged at 15,000 rpm for 5 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1% sodium dodecyl sulfate) supplemented with protease and phosphatase inhibitor cocktails (Roche, 04693159001 and 04906837001) for 30 min on ice with vortex agitation every 5-10 min ...
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bioRxiv - Microbiology 2020Quote: ... All reactions were performed in 96-well plates on a Roche LC 480 or z480 light cycler (Roche, Basel, Switzerland) according to the CDC procedure including a previously negative patient specimen (extraction and RNaseP internal controls) ...
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bioRxiv - Genetics 2021Quote: ... For whole-genome sequencing DNA was extracted using the MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche Diagnostics GmbH).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The resulting 1,055 samples of differentiated EBs were lysed after 7 days in 350 μl MagNA Pure LC RNA Buffer (Roche Diagnostics) and purified using an automated MagNA Pure 96 system (Roche Diagnostics) ...
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bioRxiv - Molecular Biology 2021Quote: ... Circular RNA levels were quantified by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), using Sybr Green mix (dssTakara, Japan) and detected by Lightcycler LC 480 (Roche). Primer sequences for qRT-PCR have been summarised in Supplementary Table 2.
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bioRxiv - Molecular Biology 2021Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) to inactivate the virus ...
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bioRxiv - Microbiology 2019Quote: ... The two primers and probe (u-16S) used to detect the 16S rRNA gene were designed with the software LC probe design2 (Roche). 16S rRNA sequences of known pathogenic or commensal species of the respiratory tract were aligned and a final selection of primers and probe was accomplished according to their universality (Figure S1) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.25□µl forward and reverse primer (to a final concentration of 250□nM, IDT) and was analysed on the LC-480 device (Roche) for RT-qPCR cycling ...
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bioRxiv - Molecular Biology 2020Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 200 μl of another aliquot of lung homogenate supernatant using the MagNA Pure Total Nucleic Acid Isolation protocol and reagents on a MagNA Pure LC 2.0 instrument (Roche Diagnostics). Prior to extraction ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplification was performed with the LC FastStart DNA master SYBR green I kit in a LightCycler 1.2 instrument (Roche Diagnostic) using the following specific primers ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Molecular Biology 2020Quote: ... TUNEL staining was performed prior to secondary antibody incubation using the Biotin-based in situ cell death detection kit (Roche) and following the manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and pBlueScript II KS reverse (5’-GCT GGG TCT CGT GGT TTC CCT TTA GTG AGG GTT AAT TG) primers and either 60 µM biotin-16-dUTP or digoxigenin-11-dUTP (Roche). This results in a 5 kb DNA tether which can be attached to a streptavidin coated magnetic bead at one end and an anti-digoxigenin coverslip surface at the other.
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bioRxiv - Bioengineering 2021Quote: Biotin-labeled dsDNA template was first amplified from the plasmid encoding the template design with biotin-labeled primers using KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for 35 cycles (98°C for 20 s ...
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bioRxiv - Synthetic Biology 2021Quote: ... the efficiency of biotinylation was checked in a Western blot revealed with POD-anti-biotin (horseradish peroxidase conjugated with Streptavidin, Roche).
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bioRxiv - Biophysics 2019Quote: ... The central fragment of the molecule is flanked by oligonucleotides labelled either with digoxigenin (3’ end) or biotin (5’ end) that specifically bind either to a glass surface covered with Anti-digoxigenin (Roche) or to superparamagnetic beads (MyOne ...
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bioRxiv - Genetics 2021Quote: ... TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling) assay was performed on retinal sections following the manuals (Cat # 11684795910, Roche).
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bioRxiv - Molecular Biology 2022Quote: ... the fragment of interest was synthesized with Q5 DNA polymerase and handles were synthesized with Taq using either biotin-16-dUTP (11093070910, Roche) or digoxigenin-11-dUTP (11093088910 ...
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bioRxiv - Cell Biology 2024Quote: ... The chromosome V was visualized with a biotin-labelled probe generated by amplifying the endogenous 5s rDNA locus by PCR and labelling was performed with the Biotin-nick translation mix (Roche) according with manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
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bioRxiv - Genomics 2023Quote: ... GenBank accession number Z69354.1) and BlSat1 (Hong Ha 2018) were labelled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich ...
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bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... and the probe pXV1 for the 5S rRNA gene (Schmidt et al. 1994) labeled with biotin-11-dUTP by PCR (Roche). The telomeric probe pLT11 from Arabidopsis (Richards & Ausubel 1988 ...
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bioRxiv - Cell Biology 2024Quote: Apoptosis was measured by Terminal Deoxynucleotidyl Transferase-Mediated dUTP-Biotin Nick End Labeling (TUNEL) assay using In Situ Cell Death Detection kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... 1% Sodium Deoxycholate and 1% Triton X-100) supplemented with Complete Protease Inhibitors without EDTA (Roche). Cellular debris were removed by centrifugation at 17,000g ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5% sodium deoxycholate containing PhosSTOP phosphatase inhibitors and a complete protease inhibitor mixture (Roche Life Science)] ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.25% Sodium Deoxycholate 0.025% SDS) supplemented with EDTA-free protease inhibitor cocktail and phosphatase inhibitors (Roche). Lysates were clarified by centrifugation ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5% sodium deoxycholate) containing PhosSTOP phosphatase inhibitors and a complete protease inhibitor mixture (Roche Life Science), then centrifuged at 20,000 g for 15min ...
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bioRxiv - Plant Biology 2022Quote: ... followed by passivation of the surface (0.5% BSA [Roche] in 10 mM sodium acetate, pH 5.0), and final quenching with 1 M ethanolamine ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% sodium deoxycholate) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and EDTA-free protease inhibitor cocktail (Roche)) on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.1% sodium dodecyl sulfate) supplemented with 2% v/v protease inhibitor cocktail (Roche; Catalog #11697498001) and 1% v/v phosphatase inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM sodium butyrate and 0.1% Igepal) supplemented with protease (cOmplete™ Protease Inhibitor Cocktail, Roche) and phosphatase (PhosSTOP ...