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Citations for Roche :
301 - 350 of 537 citations for Sulfo NHS LC Biotin sodium since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The resulting 1,055 samples of differentiated EBs were lysed after 7 days in 350 μl MagNA Pure LC RNA Buffer (Roche Diagnostics) and purified using an automated MagNA Pure 96 system (Roche Diagnostics) ...
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bioRxiv - Molecular Biology 2021Quote: ... Circular RNA levels were quantified by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), using Sybr Green mix (dssTakara, Japan) and detected by Lightcycler LC 480 (Roche). Primer sequences for qRT-PCR have been summarised in Supplementary Table 2.
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bioRxiv - Molecular Biology 2021Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) to inactivate the virus ...
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bioRxiv - Microbiology 2019Quote: ... The two primers and probe (u-16S) used to detect the 16S rRNA gene were designed with the software LC probe design2 (Roche). 16S rRNA sequences of known pathogenic or commensal species of the respiratory tract were aligned and a final selection of primers and probe was accomplished according to their universality (Figure S1) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.25□µl forward and reverse primer (to a final concentration of 250□nM, IDT) and was analysed on the LC-480 device (Roche) for RT-qPCR cycling ...
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bioRxiv - Molecular Biology 2020Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 200 μl of another aliquot of lung homogenate supernatant using the MagNA Pure Total Nucleic Acid Isolation protocol and reagents on a MagNA Pure LC 2.0 instrument (Roche Diagnostics). Prior to extraction ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplification was performed with the LC FastStart DNA master SYBR green I kit in a LightCycler 1.2 instrument (Roche Diagnostic) using the following specific primers ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Molecular Biology 2020Quote: ... TUNEL staining was performed prior to secondary antibody incubation using the Biotin-based in situ cell death detection kit (Roche) and following the manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and pBlueScript II KS reverse (5’-GCT GGG TCT CGT GGT TTC CCT TTA GTG AGG GTT AAT TG) primers and either 60 µM biotin-16-dUTP or digoxigenin-11-dUTP (Roche). This results in a 5 kb DNA tether which can be attached to a streptavidin coated magnetic bead at one end and an anti-digoxigenin coverslip surface at the other.
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bioRxiv - Bioengineering 2021Quote: Biotin-labeled dsDNA template was first amplified from the plasmid encoding the template design with biotin-labeled primers using KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for 35 cycles (98°C for 20 s ...
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bioRxiv - Synthetic Biology 2021Quote: ... the efficiency of biotinylation was checked in a Western blot revealed with POD-anti-biotin (horseradish peroxidase conjugated with Streptavidin, Roche).
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bioRxiv - Biophysics 2019Quote: ... The central fragment of the molecule is flanked by oligonucleotides labelled either with digoxigenin (3’ end) or biotin (5’ end) that specifically bind either to a glass surface covered with Anti-digoxigenin (Roche) or to superparamagnetic beads (MyOne ...
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bioRxiv - Genetics 2021Quote: ... TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling) assay was performed on retinal sections following the manuals (Cat # 11684795910, Roche).
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bioRxiv - Molecular Biology 2022Quote: ... the fragment of interest was synthesized with Q5 DNA polymerase and handles were synthesized with Taq using either biotin-16-dUTP (11093070910, Roche) or digoxigenin-11-dUTP (11093088910 ...
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bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
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bioRxiv - Genomics 2023Quote: ... GenBank accession number Z69354.1) and BlSat1 (Hong Ha 2018) were labelled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich ...
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bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... and the probe pXV1 for the 5S rRNA gene (Schmidt et al. 1994) labeled with biotin-11-dUTP by PCR (Roche). The telomeric probe pLT11 from Arabidopsis (Richards & Ausubel 1988 ...
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bioRxiv - Cell Biology 2024Quote: ... The chromosome V was visualized with a biotin-labelled probe generated by amplifying the endogenous 5s rDNA locus by PCR and labelling was performed with the Biotin-nick translation mix (Roche) according with manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... 1% Sodium Deoxycholate and 1% Triton X-100) supplemented with Complete Protease Inhibitors without EDTA (Roche). Cellular debris were removed by centrifugation at 17,000g ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5% sodium deoxycholate containing PhosSTOP phosphatase inhibitors and a complete protease inhibitor mixture (Roche Life Science)] ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.25% Sodium Deoxycholate 0.025% SDS) supplemented with EDTA-free protease inhibitor cocktail and phosphatase inhibitors (Roche). Lysates were clarified by centrifugation ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5% sodium deoxycholate) containing PhosSTOP phosphatase inhibitors and a complete protease inhibitor mixture (Roche Life Science), then centrifuged at 20,000 g for 15min ...
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bioRxiv - Plant Biology 2022Quote: ... followed by passivation of the surface (0.5% BSA [Roche] in 10 mM sodium acetate, pH 5.0), and final quenching with 1 M ethanolamine ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% sodium deoxycholate) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and EDTA-free protease inhibitor cocktail (Roche)) on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM sodium butyrate and 0.1% Igepal) supplemented with protease (cOmplete™ Protease Inhibitor Cocktail, Roche) and phosphatase (PhosSTOP ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium deoxycholate) supplemented with 1 mM PMSF and 1× protease inhibitor cocktail (Roche; Cat# 04693132001). The cells were broken using Zirconia/Silica beads (BioSpec ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 % sodium deoxycholate and 1 % NP-40) supplemented with cOmplete mini EDTA-free protease inhibitors (Roche). After protein quantification using bicinchoninic acid assay (BCA ...
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bioRxiv - Microbiology 2023Quote: ... 1% sodium deoxycholate and 1% Triton X-100) supplemented with Complete protease inhibitors without EDTA (Roche). Cellular debris was removed by centrifugation at 17,000 × g ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.1% sodium dodecyl sulfate) supplemented with 2% v/v protease inhibitor cocktail (Roche; Catalog #11697498001) and 1% v/v phosphatase inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted and purified with the MagNA Pure LC instrument and the MagNA Pure DNA isolation kit III (Roche Diagnostics), according to the recommendations of the manufacturer ...
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bioRxiv - Immunology 2019Quote: ... Steady-state LCs were enzymatically digested from the epidermal sheets using Liberase™ TM research grade (Roche, UK, 2h at 37℃).
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bioRxiv - Plant Biology 2020Quote: ... The sites of hybridization of digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science, Penzberg, Germany) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Biotin-labeled LPA1-HA was detected using streptavidin beads (Pierce) and conjugated anti-HA antibody (3F10 from Roche Diagnostics; 1:1000).
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bioRxiv - Plant Biology 2022Quote: ... Hybridization signals of probes labelled with digoxigenin-11-dUTP and biotin-16-dUTP were detected with antidigoxigenin conjugated to fluorescein isothiocyanate (FITC; Roche Diagnostics) and streptavidin conjugated to Alexa 594 or Alexa 647 (Life Technologies-Molecular Probes) ...
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bioRxiv - Immunology 2023Quote: ... Apoptosis also was detected using a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assay with Cell Death Detection Kit (Roche, Germany). Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2023Quote: Antisense and sense digoxigenin- and biotin-labeled riboprobes of AcerOr11 were synthesized using linearized pGEMHE plasmids containing appropriate insertion sequences as a template using the DIG and Biotin RNA Labeling Mix (Roche, Germany) and T7 RNA Polymerase (Roche ...
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bioRxiv - Immunology 2021Quote: Sorted cells or stimulated cells were lysed in RIPA buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM PMSF, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1x Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2020Quote: ... 1% sodium deoxycholate and 1% Triton X-100) supplemented with 1xEDTA-free complete protease inhibitor mixture (Roche) and phosphatase inhibitor cocktails-I ...
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bioRxiv - Developmental Biology 2021Quote: ... and then lysed in RIPA buffer (50 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.5% NP40, and 0.25% sodium deoxycholate, supplemented with protease and phosphatase inhibitors; Roche). Proteins were immunoprecipitated by the addition of 3 µl of rabbit anti-FGFR3 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was extracted by adding ice-cold 10 v/w RIPA buffer (250 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris HCl, pH 8.0, containing Roche cOmplete™ protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail and 1mM AEBSF ...
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bioRxiv - Cancer Biology 2022Quote: ... and 49 were resuspended in 25μL 10% sodium dodecyl sulfate solution with a protease inhibitor cocktail (Roche). Reduction of disulfides was performed by adding dithiothreitol (DTT ...
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bioRxiv - Microbiology 2020Quote: ... 5 mM EDTA, 0.10% NP40, 0.5 mM sodium orthovanadate, 0.5 mM NaF, protease inhibitor cocktail from Roche) and reduced in the presence of β-mercaptoethanol by boiling at 95°C for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1% sodium dodecyl sulfate) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and proteinase and phosphatase inhibitor cocktails (Roche). The protein extract was centrifuged at 25,000g for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... followed by clean-up of RNA by phenol/chloroform/isoamyl alcohol and precipitation of RNA with 2.5 volumes of ethanol containing 0.1 M sodium acetate pH 5.5 and 20 µg of glycogen (Roche)[57] ...
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bioRxiv - Microbiology 2019Quote: ... RNA was precipitated overnight with 2.5 volumes of ethanol/0.1M sodium acetate (pH 5.5) and 20 µg of glycogen (Roche) mixture ...
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bioRxiv - Microbiology 2019Quote: ... Cells were resuspended in 100 µl of ice cooled lysis Buffer I (150 mM sodium chloride, 1.0% NP-40, 0.5% sodium deoxycholate, 50 mM Tris-HCl, pH 7.5 and protease inhibitor [Roche]), vortexed for 5 seconds ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1.0% IGEPAL CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0 protease inhibitor cocktail [Roche]) and shook on ice for 15 min ...