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Citations for Roche :
301 - 350 of 664 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 5 mM MgCl2, 0.1 M EDTA, 10 % glycerol and 0.5 mM DTT, supplemented with a protease inhibitor cocktail from Roche) or Trap buffer (10 mM HEPES pH = 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... Tissues were ground with mortor and pestle and resuspended in 30 mL extraction buffer 1 (10 mM Tris-HCl, pH 8.0, 5 mM βmercaptoethanol, 0.4 M sucrose, protease inhibitor cocktail (cOmplete, Roche), vortexed ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were stopped by adding 0.5 μl ethylenediaminetetraacetic (0.5 M EDTA) and 1 μl Proteinase K (19 mg/ml, Roche), and incubated at 50°C for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse brain samples were lysed in fresh lysis buffer (50 mM HEPES, pH 8.5, 8 M urea, 0.5% sodium deoxycholate and phosphatase inhibitor cocktail (PhosphoSTOP, Roche). The protein concentration of the lysate was quantified by BCA protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Bound protein was eluted by heating immunoglobulin G beads with 35 μl of denaturing urea SDS-loading dye (1% SDS, 8 M urea, 10 mM Tris, pH 6.8, 10 mM EDTA, 0.01% bromophenol blue, 1x Roche Complete inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.2 mM EDTA, 25% glycerol, 0.42 M NaCl, 2 mM beta-mercaptoethanol, 0.01% IGEPAL CA-630, 2× Roche cOmplete protease inhibitors ...
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bioRxiv - Cell Biology 2024Quote: ... and sperm were prepared by homogenization in cell lysis buffer (04719964001, cOmplete™ Lysis-M EDTA-free, Roche) and diluted with an equal volume of sample buffer solution containing 2-ME (2 × ...
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Transmembrane domains of H1 and H3 hemagglutinin contribute to membrane fusion in a different mannerbioRxiv - Biophysics 2024Quote: Plant leaf tissue was weighed and homogenized in the isolation buffer (0.1 M sodium phosphate, pH 7.2) supplemented with a protease inhibitor cocktail (cOmplete, Roche) using a Waring blender ...
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bioRxiv - Cell Biology 2024Quote: ... 10 mM KCl, 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM dithiothreitol [DTT], a protease inhibitor cocktail [Roche]). Triton X-100 (0.1% ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Cross-linked membranes were blocked in pre-warmed DIG EasyHyb (Roche # 11603558001) at 43°C for four hours rolling in hybridization tubes ...
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bioRxiv - Molecular Biology 2020Quote: PCR products were transferred to a positively charged nylon membrane (Roche, Switzerland) using capillary transfer under denaturing conditions ...
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bioRxiv - Neuroscience 2020Quote: ... the proteins were transferred onto a PVDF membrane (Roche Diagnostics, Mannheim, Germany). Cofilin1 and phospho-Cofilin1 (Ser3 ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were pre-hybridized in DIG Easy Hyb solution (Roche, Catalog# 11603558001) for 30 minutes at 40°C followed by overnight incubation at 40°C with 1 μg/ml DIG-labeled DNA probes in DIG Easy Hyb solution with continuous agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were briefly equilibrated with 10 mL 1x Detection Buffer (Roche, 115857262001). To detect DIG-labeled probing ...
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bioRxiv - Plant Biology 2022Quote: ... The membrane was incubated with anti-GFP primary-(Roche #11814460001/1:2000) and anti-mouse (Cell Signaling Technology #7076/1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were immunostained with mouse anti-GFP (1:250 dilution; Roche, 11814460001), rabbit anti-Aldolase-HRP conjugated (1:5000 dilution ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the membrane was blocked with a 1% blocking solution (Cat. 11096176001, Roche) for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were pre-hybridized with DIG Easy Hyb solution (Roche, REF: 11603558001) for 1h at 42 °C ...
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bioRxiv - Genetics 2020Quote: ... fragments were transferred from the gel to a charged nylon membrane (Roche). A LYS2 probe was amplified and labeled with Digoxigenin (DIG)-dUTP using the primers 5’-TGAAGCCTTCCCAGAGAGAA and 5’-GCCAAGGAAAAATGTCTACCA (Roche PCR DIG Probe Synthesis Kit) ...
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bioRxiv - Neuroscience 2020Quote: ... separated on a 10% SDS-PAGE and blotted onto PVDF membranes (Roche). Membranes were blocked with 5% skim milk/PBST ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were pre-hybridized with DIG Easy Hyb buffer (Roche, Switzerland) for at least 1 h at 45°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and immediately imaged and transferred to positively charged nylon membranes (Roche, Switzerland). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... and membrane washing were conducted using the DIG luminescent Detection kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were exposed to chemiluminescence detection films (Roche Diagnistics, Rotkreuz, Switzerland). Detection of anti-actin served as loading control for the lysate.
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bioRxiv - Molecular Biology 2024Quote: ... and RNA was released from the membrane using Proteinase K (Roche, 03115828001). RNA was purified using neutral phenol/chloroform/isoamylalcohol (Ambion ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were exposed to chemiluminescence detection films (Roche Diagnistics, Rotkreuz, Switzerland). Detection of actin served as loading control for the lysate.
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bioRxiv - Biochemistry 2023Quote: ... The membrane was then blocked with Blocking solution (1% Blocking reagent, Roche 11096176001 ...
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bioRxiv - Biochemistry 2024Quote: ... and RNA was released from the membrane using Proteinase K (Roche, 03115828001). RNA was purified using neutral phenol/chloroform/isoamylalcohol (Ambion ...
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bioRxiv - Plant Biology 2023Quote: ... then blotted onto a positively charged membrane (Roche Diagnostics, West Sussex, UK) and fixed using ultraviolet cross-linking ...
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bioRxiv - Cell Biology 2023Quote: ... cytospins and membranes were permeabilized by 0.1% Triton X-100 (Roche, SIG10789704001) for 15 min and blocked with 2% BSA in PBS for 1 hour at room temperature (RT) ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were probed with rat anti-HA (clone 3F10, Roche; 1:1,000) followed by 1:20,000 IRDye 680RD Goat anti-Rat IgG secondary antibody (LI-COR Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... the DNA samples were transferred to a positively charged nylon membrane (Roche) through reverse capillary action overnight with 10 × saline-sodium citrate buffer (SSC ...
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bioRxiv - Plant Biology 2023Quote: ... The blotted membrane was pre-hybridized in DIG Easy Hybridization solution (Roche) at 42°C for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... RNA from the gel was then blotted on a nylon membrane (Roche) at 125 mA at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were blocked in 5% BSA (Roche, 10 735 086 001) in TBS-T before overnight incubation at 4°C with primary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... blotted onto a PVDF membrane and probed with α-HA tag (Roche, Catalog# 11867431001 ...
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bioRxiv - Microbiology 2024Quote: ... membrane was hybridized with 75 mU/ml Anti-Digoxigenin-AP (#11093274910, Roche) in blocking solution for 60 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... the membranes were treated with Lumi-LightPLUS Western Blotting substrate (Roche, Switzerland) for signal development and visualized using a ChemiDoc XRS+ station (Bio-Rad) ...
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bioRxiv - Genomics 2024Quote: ... membranes were probed with antibodies against myc (Roche 11-667-203-001), Pab1 (a kind gift from Maurice Swanson of University of Florida ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplified and control reactions were blotted onto positively charged nylon membrane (Roche) and crosslinked ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were suspended in 50 ml of denaturation buffer (8 M GdmCl, 50 mM Tris-HCl, pH 8.0) with one protease inhibitor tablet (Roche) per L medium ...
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bioRxiv - Neuroscience 2021Quote: Frozen mouse hippocampi were homogenized in 0.2 ml of pre-cooled homogenization buffer (0.3 M sucrose, 10 mM MOPSNaOH and 1mM EDTA) supplemented with complete protease inhibitor cocktail (Roche). 50 µl of lysis buffer (8% SDS and 0.2 M DTT in 0.2 M Tris-HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in proteome lysis buffer (50 mM Tris HCl pH 8.0, 8 M urea, 75 mM NaCl, 100 mM sodium butyrate, cOmplete protease inhibitors – Roche). Cells were then mechanically lysed (MagNAlyzer Roche – 6000 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were resuspended and bead beaten in 250 μL urea buffer (6 M urea, 1% SDS, 50 mM Tris-HCl pH 6.8, 1 mM EDTA, 2x Roche protease inhibitors ...
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bioRxiv - Biochemistry 2020Quote: ... Elution buffer (50 mM Na2HPO4, 0.3 M NaCl, 250 mM imidazole; pH 8) containing EDTA-free protease inhibitors (cOmplete, Roche) was used to elute the proteins ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was diluted with 50 mM ABC down to 0.5 M urea and then digested overnight using sequencing-grade endoproteinase GluC (Roche) at 25 °C ...
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bioRxiv - Biophysics 2020Quote: ... a 500 mL cell pellet of Sf9 cells was thawed in 50 mL lysis buffer (25 mM HEPES pH 7.4, 1 M KCl, 10% glycerol, Roche protease inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 3 ml S1 solution (0.25 M sucrose, 10 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) by pipetting up and down ...
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bioRxiv - Biophysics 2022Quote: ... 0.3 M NaCl) containing either 200 µM PMSF or a tablet of complete EDTA-free protease-inhibitor cocktail (Roche) and 10 ng ml−1 DNAse I ...
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bioRxiv - Cell Biology 2021Quote: Cell pellets or mouse tissues were resuspended in ice-cold 8 M urea buffer (8 M urea, 50 mM Tris pH 7.4, 50 mM NaCl) supplemented with protease and phosphatase inhibitors (Roche). The resuspended samples were sonicated ...