Labshake search
Citations for Roche :
301 - 350 of 2419 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... 0.15 M NaCl and complete protease inhibitor cocktail (Roche) by ultrasonication ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.3 M sucrose containing Complete Protease Inhibitor Cocktail (Roche). Suspended cells were homogenized using a syringe with a 27-gauge needle ...
-
bioRxiv - Immunology 2023Quote: ... Initial T cell activation was performed using anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10 μg/mL Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentiviral transduction was performed by inoculating cells with Vectofusin 1 (10 μg/mL ...
-
bioRxiv - Cell Biology 2022Quote: ... from BD Biosciences, mAb anti-HA-tag (11867423001; IF: 1/500, WB: 1/2000) from Roche, mAb anti-mRFP/DsRed2 (sc-101526 ...
-
bioRxiv - Microbiology 2023Quote: ... Amplified fragments with adapters and tags were purified using KAPA Pure beads (Roche, Switzerland) at a 1:1 concentration.
-
bioRxiv - Microbiology 2020Quote: ... coli Novablue were extracted using the High Pure RNA Isolation Kit (Roche) following the manufacturer’s protocol ...
-
bioRxiv - Genetics 2019Quote: ... coli strains using the High Pure plasmid isolation kit (Roche, Basel, Switzerland). PCR amplification of short DNA fragments (<3000 bp in length ...
-
bioRxiv - Microbiology 2020Quote: Escherichia coli MRE600 total transfer ribonucleic acid was purchased from Roche (Switzerland). Biotin labeled single-stranded DNA oligonucleotides were obtained from B.G.I. ...
-
bioRxiv - Microbiology 2020Quote: ... 0.5 M β-mercaptoethanol) supplemented with protease inhibitor cocktail (Roche) and sonicated for 10 s at 20% amplitude using a Sonics Vibra Cell sonicator fitted with a microprobe ...
-
bioRxiv - Biophysics 2022Quote: ... 0.5 M sucrose) supplemented with one protease inhibitor tablet (Roche). Osmotic shock was performed by the addition of diluted TES buffer to release the periplasmic proteins ...
-
bioRxiv - Biophysics 2022Quote: ... 0.5 M sucrose) supplemented with one protease inhibitor tablet (Roche). Osmotic shock was performed by the addition of diluted TES buffer to release the periplasmic proteins ...
-
bioRxiv - Biophysics 2022Quote: ... 0.02% NaN3 and 1x cOmpleteT M Protease Inhibitor Cocktail (Roche). 10% D2O was added for lock.
-
bioRxiv - Microbiology 2023Quote: ... and suspended in 8 M urea containing PhosSTOP (Roche Diagnostics) and Benzonase (Novagen) ...
-
bioRxiv - Microbiology 2023Quote: ... 0.5 M β-mercaptoethanol) supplemented with protease inhibitor cocktail (Roche) and lysates were sonicated for 10 s at 20% amplitude using a Vibra-Cell sonicator (Sonics ...
-
bioRxiv - Microbiology 2019Quote: ... 1 mM ATP (+ 2 mM DTT for Strep-KaiC36803 proteins)] including protease inhibitors (protease inhibitor cocktail, Roche or Nacalai). Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences ...
-
bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... followed by PCR amplification with KAPA 2X Hi-Fi Hotstart Readymix (Roche, Cat. No. KR0370).
-
bioRxiv - Biochemistry 2020Quote: ... Mouse monoclonal antibody raised against hemagglutinin (HA) tag (clone 12CA5, #11583816001) was obtained from Roche. Mouse monoclonal antibody raised against MYC tag (9B11 ...
-
bioRxiv - Physiology 2022Quote: ... Plasma was collected and measured for total TAGs concentration using commercial enzymatic kits from Roche Diagnostics (10166588130 ...
-
bioRxiv - Synthetic Biology 2019Quote: ... coli total tRNA mixture (from strain MRE600) and phosphoenolpyruvate were purchased from Roche Applied Science ...
-
bioRxiv - Synthetic Biology 2020Quote: ... coli total tRNA mixture (from strain MRE600) and PEP was purchased from Roche Applied Science (Indianapolis ...
-
bioRxiv - Cell Biology 2020Quote: ... coli liquid cultures using the High Pure plasmid isolation kit (Roche, Basel, Switzerland).
-
bioRxiv - Microbiology 2022Quote: ... At the end of the cell disruption dry homogenised powder of cells were resuspended in 1 ml urea buffer (8 M urea, 50 mM Tris/HCl pH 8.0 containing one tablet of ‘cOmplete’ protease inhibitor per 25 ml, Roche, Germany; 1 mM DTT; 0.1 M PMSF) and centrifuged at 16,000 × g for 10 minutes at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: ... GTP binding Irgb6 crystals diffracting to 1.5 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GTP (Roche) and a reservoir solution consisting of 0.1 M Sodium Citrate buffer pH 5.4 (Wako) ...
-
bioRxiv - Microbiology 2022Quote: ... GTP-binding Irgb6-T95D crystals diffracting to 1.68 Å resolution were obtained from sitting drops with a 0.5 μl of protein solution containing 2 mM GTP (Roche) and a 0.5 μl of reservoir solution consisting of 0.2 M Sodium sulfate (Molecular Dimensions ...
-
bioRxiv - Plant Biology 2022Quote: Total protein extracts were prepared by re-suspending 100 mg of ground plant material in 100 μl of protein lysis buffer (50 mM Tris pH 8.0; 2% SDS; 10 mM EDTA; protease inhibitors (Roche (04693159001) cOmplete™ ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.32 M sucrose supplemented with Complete mini protease inhibitors (# 11836153001, Roche), PhosStop phosphatase inhibitor cocktail (# 4906837001 ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.25 M sucrose and EDTA-free protease inhibitors (Roche, cat# 05056489001) followed by centrifugation at 9000 rpm for 15 minutes ...
-
bioRxiv - Cell Biology 2022Quote: Cell lysates were prepared using cOmplete™ Lysis-M (4719956001, Roche) with protease and phosphatase inhibitors and subjected to standard electrophoresis and transfer as previously described41 ...
-
bioRxiv - Neuroscience 2024Quote: ... 0.01 M Tris-HCl pH 7.4] containing protease inhibitors (Roche, Switzerland). For sarkosyl preparations ...
-
bioRxiv - Bioengineering 2019Quote: ... DNA was amplified using the polymerase Kapa Hi Fi DNA polymerase (Kapa Biosystems, cat no. KK2602) and gel purified using Seakem GTG agarose (for nucleic acid recovery ...
-
bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
-
bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
-
bioRxiv - Cell Biology 2021Quote: Commercially available primary antibodies used for western blot are rat anti-HA tag (clone 3F10; Roche), rabbit anti-Cyclin B1 (4138S ...
-
bioRxiv - Plant Biology 2022Quote: ... 5 mM imidazole and 500 μL cOmplete Hig-tag purification Resin (obtained from Roche, ref 05893682001). The mixture was gently shaken at 4°C for 4 h in dark ...
-
bioRxiv - Microbiology 2022Quote: ... Primary antibodies used (at 1/1,000, unless specified) were rat anti-HA tag (clone 3F10, Roche), mouse anti-TY tag (27) ...
-
bioRxiv - Immunology 2024Quote: ... and bacterial communities were analyzed by bacterial tag encoded FLX amplicon pyrosequencing (Roche, Branford, CT, USA) as previously described [35,36] ...
-
bioRxiv - Immunology 2020Quote: ... 14 and 21 of life were homogenized in protein lysis buffer (Tris 50 mM, 2% SDS, 1mM Na3VO4) containing proteinase inhibitors (Complete Mini, Roche Diagnostics) and benzonase (Novagen ...
-
bioRxiv - Cell Biology 2022Quote: ... of siCon or siRictor NIH3T3 cells co-expressing Flag-NDRG1 WT were homogenized in 2% SDS + 5 mM DTT to retrieve proteins in solution supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... recombinant cloned from Flavobacterium meningosepticum and expressed in Escherichia coli) was purchased from Roche Diagnostics (Mannheim ...
-
bioRxiv - Developmental Biology 2021Quote: ... F0425)/500 µg protein with protein A-agarose (Roche). Fished-out FGFR3 protein was eluted and then denatured at 95°C for 10 minutes in NuPAGE LDS sample buffer (Life Technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... pH 7.4 plus 0.32 M sucrose supplemented with protease inhibitor cocktail (Roche). To isolate synapse-enriched fractions ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 m DTT) supplemented with protease inhi itor ta lets (Roche, 11697498001) and 75 U/ml of Pierce universal nuclease (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... 8 M Urea loading dye was supplemented with complete protease inhibitor (Roche). 100 μl Urea loading dye were used to resuspend cell pellet after NaOH treatment ...
-
bioRxiv - Developmental Biology 2020Quote: ... Extracts were made using Complete Lysis-M EDTA-Free Lysis buffer (Roche). Antibody Array was performed ...
-
bioRxiv - Biochemistry 2024Quote: ... 8 M Urea and 1x cOmpleteTM EDTA-free protease inhibitor (Roche #11873580001). Lysates were sonicated with a probe sonicator and cleared via centrifugation at 15,000x g for 30 min ...
-
bioRxiv - Physiology 2022Quote: ... Commercially available enzymatic kits were used to measure serum concentrations of TAGs (Roche Diagnostics, Woerden, the Netherlands) and FFAs (Wako chemicals ...
-
bioRxiv - Cell Biology 2024Quote: ... primary antibody incubation was performed in blocking buffer with anti-HA-tag (rat, Roche, 11867423001, 1:250) or anti-CD9 (mouse ...
-
bioRxiv - Microbiology 2021Quote: Viral RNA of all VSV-spike pseudovirus particles were extracted from 200ul supernatant using the High Pure Viral RNA Kit (Roche, Cat. No. 11858882001) following the supplier’s manual ...