Labshake search
Citations for Roche :
301 - 350 of 6709 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Bacterial pellets were collected via centrifugation at 4°C and 5,000g and resuspended in ice-cold lysis buffer (50 mM MES pH 6.8, 1 mM EGTA, 0.2 mM MgCl2, 5 mM DTT, Roche complete protease inhibitor ...
-
bioRxiv - Molecular Biology 2023Quote: ... and lysed in NP-40 buffer (150 mM NaCL, 1% IGEPAL, 50 mM Tris-Cl p.H. 7.5) with protease inhibitor (Roche #04693132001 ...
-
bioRxiv - Cell Biology 2022Quote: ... Beads were incubated with immature and mature DC lysates prepared in RIPA buffer (10 mM HEPES, 50 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, protease inhibitor cocktail (Roche), 1 mM Na3VO4,1 mM DTT ...
-
bioRxiv - Genomics 2022Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
-
bioRxiv - Plant Biology 2022Quote: ... incubated with proteinase K (1 mg/mL in 100 mM Tris-HCl, pH 8.0, 50 mM EDTA; Roche) at 37 °C for 30 min ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... planulae were washed twice with 1/3 strength artificial seawater and incubated with 50 μg/mL liberaseTM (Roche) at 37 °C for 10–20 min with occasional pipetting ...
-
bioRxiv - Cell Biology 2023Quote: ... 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 50 mM sodium fluoride, protease inhibitors [Roche]). Triton X-100 (0.1% ...
-
bioRxiv - Cell Biology 2024Quote: ... washed with cold PBS and then lysed for 4 hours on rotation at 4°C in TNEN buffer (50 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.1% NP-40, Protease inhibitors (Roche) and phosphatase inhibitors (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) supplemented with complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche) and 1 mg/ml lysozyme (Sigma) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 50 mM Tris-HCl pH 8.0, 5 mM EDTA, 0.5 % SDS, 1#x00D7; protease inhibitor cocktail from Roche). The resulting nuclei were spun down ...
-
bioRxiv - Biochemistry 2024Quote: ... Frozen cell pellets were resuspended in Lysis Buffer (50 mM HEPES [pH 7.5], 100 mM NaCl, 1 mM NaF, 0.1% βME with Roche EDTA-free protease inhibitor tablets ...
-
bioRxiv - Genetics 2024Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
-
bioRxiv - Cancer Biology 2024Quote: ... and resuspended for lysis in RIPA buffer (50 mM Tris pH 7.6, 150mM NaCl, 1% Triton X-100, 0.1% SDS) with protease (04693159001, Roche) and phosphatase inhibitors (524625 ...
-
bioRxiv - Immunology 2022Quote: ... were coated O/N at 4 °C with 50 μl of fibronectin (50 μg/ml; Roche) or VCAM-1-Fc (10 μg/ml R&D System) ...
-
bioRxiv - Immunology 2022Quote: ... and Liberase (50 µg/ml; Roche), minced and agitated at 37°C for 30 min ...
-
bioRxiv - Immunology 2022Quote: ... DNase I (50 µg/ml, Roche) for 30 min at 37°C with agitation ...
-
bioRxiv - Immunology 2021Quote: ... 50 µg/ml DNAse I (Roche)) for 30 min in a shaking incubator at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... and DNase (50 U/ml, Roche) at 37 °C for 45-60 min ...
-
bioRxiv - Neuroscience 2021Quote: ... random hexamer primers (50 µM; Roche), RNasin (20 U ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 50 μg/ml DNAseI (Roche). Dissociated samples were filtered through a 70-μm cell strainer ...
-
bioRxiv - Genetics 2020Quote: ... and 50 µL Proteinase K (Roche) was added and the solution was vortexed ...
-
bioRxiv - Immunology 2020Quote: ... 50 µg/mL DNase I (Roche)) ...
-
bioRxiv - Immunology 2021Quote: ... and 50 U/ml DNase (Roche) were added ...
-
bioRxiv - Molecular Biology 2023Quote: ... 50 μg/mL RNase A (Roche) and 10 μg/mL DNase (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... and 50 μg/mL DNAseI (Roche).
-
bioRxiv - Immunology 2022Quote: ... IL-2 (50 IU/ml) (Roche) and glucose (Sigma) ...
-
bioRxiv - Cancer Biology 2022Quote: ... with 50 U/mL DNase (Roche) and 1 mg/mL CaCl2 (10 mL of digestion media per gram of tissue) ...
-
bioRxiv - Physiology 2023Quote: ... and 50 ng/ml DNAseI (Roche) at 37°C for 30 min to create a single-cell suspension ...
-
bioRxiv - Immunology 2023Quote: ... 50 μg/mL DNAse I (Roche Diagnostics ...
-
bioRxiv - Microbiology 2024Quote: ... and 50 µg/ml DNAse (Roche) for 45min shaking at 37°C ...
-
bioRxiv - Plant Biology 2023Quote: ... Total proteins were extracted by mixing 500 mg of ground tissue with 2.0 ml of extraction buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, cOmplete Mini EDTA-free Protease Inhibitor Cocktail [Roche, 1 tablet per 10 ml]), followed by brief sonication in an ultrasonic bath for 5 s ...
-
bioRxiv - Cancer Biology 2021Quote: ... The cell pellets were then resuspended in 400 uL Lysis buffer (1% SDS, 50 mM Tris Hcl pH 8.0 20 mM EDTA, protease inhibitors (Roche) and incubated on ice for 10 minutes ...
-
bioRxiv - Developmental Biology 2021Quote: ... in liquid nitrogen in a 1.5-ml Eppendorf tube and resuspended in ice-cold lysis buffer (50 mM Tris pH 7.6, 10 mM EDTA, 150 mM NaCl, 1% Triton, supplemented with complete protease inhibitor, Roche Ref ...
-
bioRxiv - Cell Biology 2020Quote: ... Magnetic beads were washed 2 times with lysis buffer and 4 times with washing buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mg/ml Pefabloc SC (Roche), and EDTA-free protease inhibitor cocktail (cOmplete Tablets ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2% blocking powder from Roche, 5X SSC, 0.1% TritonX, 0.1% CHAPS from Sigma Aldrich, 50% formamide, 1 mg/ml tRNA from Roche, 5 mM EDTA from Sigma and 50 μg/ml Heparin from Sigma ...
-
bioRxiv - Developmental Biology 2020Quote: ... 10 µl reaction mix was mixed with 290µl IP buffer (1% NP-40, 50 mM Tris-HCl, pH 8.0, 150 mM NaCl) containing protease inhibitor cocktail tablet (Roche) and 2 µg/ml of anti-FLAG (Sigma) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Column was washed three times with wash buffer (PBS 2X, Triton X-100 0.1%, imidazole 50 mM, NaCl 500 mM, DTT 1 mM, 1x Complete protease inhibitor [Roche]) before storage in 50% glycerol (0.01 % Triton) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Samples were incubated with 50 μl of 1:10 Enzyme:TUNEL reagent (TdT and fluorescein-dUTP) (Roche, Cat No. 11684795910) at 37°C for 3 h in the dark ...
-
bioRxiv - Cell Biology 2020Quote: ... before lysing in lysis buffer ((1% Triton X100, 50 mM HEPES, 150 mM KCl, 1 mM MgCl2, pH 7.4, with 1x EDTA-free protease inhibitors, Roche) at 4 °C for 2 hrs ...
-
bioRxiv - Biochemistry 2020Quote: ... The medium was discarded and the cells were resuspended in 200 µL of ice-cold 50 mM HEPES buffer pH 7.4 containing 1 x protease inhibitor cocktail (Roche) and 1 mM phenylmethylsulfonyl fluoride ...
-
bioRxiv - Neuroscience 2020Quote: ... The ipsi- and contralateral cortices were immediately transferred to lysis buffer (50 mM Tris, pH 7.6, 150 mM NaCl, 1% Triton X-100, CompleteMini Protease Inhibitor Mixture, Roche). The cortices were homogenized and incubated on ice for 1 hour ...
-
bioRxiv - Microbiology 2020Quote: ... pellets were re-suspended in 1.2 mL of Lysis Buffer (50 μg/ml lysozyme, 0.8% NP-40, 1 mM MgCl2, 1x protease inhibitors [Roche; cOmplete Protease Inhibitor Cocktail] in PBS) ...
-
bioRxiv - Plant Biology 2021Quote: ... and 250 µl extraction buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 % Nonidet P40, proteinase inhibitor cocktail (Roche)) per 200 mg tissue powder was added ...
-
bioRxiv - Cancer Biology 2020Quote: ... 150 mM NaCl, 50 mM Tris pH 8.5, 40 mM chloroacetamide, 5 mM TCEP; freshly added: 1 tablet Phosphostop [Roche, 4906845001] and 1 tablet cOmplete Protease inhibitor cocktail [Roche ...
-
bioRxiv - Biophysics 2020Quote: ... a 500 mL cell pellet of Sf9 cells was thawed in 50 mL lysis buffer (25 mM HEPES pH 7.4, 1 M KCl, 10% glycerol, Roche protease inhibitor ...
-
bioRxiv - Cell Biology 2021Quote: ... The washed beads were resuspended in 200 μL 50 mM ammonium bicarbonate (pH 8.3) and on-bead trypsin digest (rolling with 1 μg of trypsin (Roche) for 16 h at 37°C followed by a second trypsin digest with 0.5 μg trypsin for 2 hours ...
-
bioRxiv - Developmental Biology 2022Quote: ... and then homogenized with a glass tissue homogenizer in lysis buffer (50 mM Tris pH 7.5, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate) containing protease inhibitors (Roche, 11697498001). Supernatant fractions were collected by centrifugation and boiled with 2× Laemmli sample buffer (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% NP-40, 2x Roche EDTA-free protease inhibitors ...
-
bioRxiv - Cell Biology 2022Quote: Cell pellets were lysed for 30 min at 4°C with end-over-end mixing in cold lysis buffer [50 mM tris pH 7.5 | 150 mM NaCl | 1 mM EDTA | 0.5% NP-40 | 1x protease (Roche, 11836170001) and phosphatase (Roche ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 μL of bromophenol blue and 50 mM DTT) containing 2 mM PMSF and cOmplete protease inhibitor cocktail (Roche) according to manufacturer’s instructions ...