Labshake search
Citations for Roche :
3251 - 3300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Libraries were constructed on 150 ng of input DNA with the KAPA HyperPlus kit (Roche Sequencing, CA, USA) according to manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.45 mg/ml collagenase A (Roche#10103586001), as previously described in 70 ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5% SDS and 1x EDTA-free protease inhibitor (Roche)] using Covaris E220 with the following parameter ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA was amplified by LightCycler 480 II (Roche) system using a SYBR Green probe ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 8.5 was added and this reaction was treated with 4 Units of Alkaline Phosphatase (Roche Life Science) at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... pellets were lyzed in microfluidizer in the presence of 1 × protease inhibitor (Roche) and cleared by ultracentrifugation at 50,000 × g for 20 min ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... mice (n=2 per group) were randomly assigned to receive either diazepam (synthesized by F. Hoffmann-La Roche) 0.3 ...
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bioRxiv - Biochemistry 2024Quote: ... pH 7.5) supplemented with protease inhibitor cocktail (Roche, 11836170001). The lysate was thoroughly vortexed ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then harvested by centrifugation and resuspended in buffer A (20 mM Tris-HCl, 1 mM EDTA, pH 8.0) including a protease inhibitor tablet (EDTA-free, Roche). Cells were lysed by sonication on ice before cell debris was removed by centrifuged (18,000 rpm ...
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bioRxiv - Biochemistry 2024Quote: Protein extracts were prepared using RIPA buffer supplemented with protease inhibitors (Complete ULTRA, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Biochemistry 2024Quote: Homo sapiens cDNAs were amplified by PCR using KAPA HiFi DNA Polymerase (Kapa Biosystems) and sub-cloned into a variety of vector backbones ...
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bioRxiv - Neuroscience 2024Quote: ... containing phosphatase and protease inhibitors (Roche, 04906845001, 05056489001) (TBS+) ...
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bioRxiv - Plant Biology 2024Quote: ... 5 mM MgCl2 and 0.1% Nonidet P40) was supplemented with protease inhibitor (Roche Complete) and added to 100 mg of ground tissue ...
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bioRxiv - Neuroscience 2024Quote: ... The PCR was executed using a 384-well block on a LightCycler 480 system (Roche, Switzerland). Relative transcript levels for Pink1 and Prkn were calculated with 2-ΔΔCT [46] using Actb as housekeeping gene and normalized to the relative expression level of the corresponding tissue from 3-month-old mice ...
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bioRxiv - Plant Biology 2024Quote: ... and 1x PhosSTOP (Roche, Penzberg, Germany). Extracts were cleared by centrifugation at 5,000 g for five min ...
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bioRxiv - Neuroscience 2024Quote: ... in 20 mM HEPES + 150 mM NaCl buffer with 0.5% CHAPS and a protease inhibitor tablet (Roche), using established methods (Brunet et al. ...
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bioRxiv - Pathology 2024Quote: ... 0.1% sodium dodecyl-sulphate (SDS) and complete mini-proteinase inhibitors (Roche)) was used to homogenize tissue ...
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bioRxiv - Plant Biology 2024Quote: ... LightCycler 480 SYBR Green I Master (Roche) was used for Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2024Quote: ... was used for Quantitative RT-PCR (qRT-PCR) on a Roche LightCycler 480 system (Roche). Tubulin was used as an internal control gene ...
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bioRxiv - Plant Biology 2024Quote: ... 0.2 mg/ml Creatine Kinase (Roche Diagnostics, #10127566001) and 40% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... CSF levels of Aβ42 and Aβ40 were measured with the Roche Elecsys platform (Roche Diagnostics International Ltd. ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.5% sodium deoxycholate) was supplemented with a protease inhibitor cocktail (complete EDTA free, Roche, Basel, Switzerland). Homogenates were centrifuged at 12000 g for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... EDTA-free mini protease inhibitor cocktail (Roche), 1 mM phenylmethylsulphonyl fluoride ...
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bioRxiv - Neuroscience 2024Quote: ... Insert sizes were evaluated via Caliper GX (Perkin-Elmer, USA) and libraries quantitated with Kapa SYBR FAST Universal kit (Roche, USA). Sequencing was performed on the Illumina NovaSeq 6000 platform to generate 100M paired reads per sample at 150 nucleotide read lengths ...
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bioRxiv - Neuroscience 2024Quote: ... and DNase I (50 µg/ml, Roche Diagnostics, Indianapolis, IN, USA) at 37°C for 45 min in a shaker set at 180 revolutions per minute ...
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bioRxiv - Neuroscience 2024Quote: ... Mitomycin C (Roche, 10107409001), soluble cholesterol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated in DAPI (2 µg/ml, 10236276001, Roche Merck) in PBS (10 min) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% sodium deoxycholate) supplemented with cOmplete protease inhibitor (Roche; cat. #11873580001). Cell or brain lysates were further solubilized by sonication and 1 h rotation at 4℃ ...
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bioRxiv - Physiology 2024Quote: ... and 5 μg/mL glucose-6-phosphate dehydrogenase (Roche)) was added to each well ...
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bioRxiv - Physiology 2024Quote: ... The final pellets were suspended in 200 μL of 0.3 mg/ml amyloglucosidase (Roche) in 0.2 M sodium acetate (pH 4.8) ...
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bioRxiv - Neuroscience 2024Quote: ... and Complete mini EDTA-free protease inhibitor cocktail (Roche, #4693159001). Sample homogenates were then boiled on a heat block at 95° C for 5 min and allowed to cool to RT ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed twice more in PBS and stained with 1 μg/mL DAPI (Roche, 10236276001) in PBS for 5 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 0.5% NP-40 [Thermo Scientific, Waltham, MA, USA], EDTA-free protease inhibitor [Roche, Basel, Switzerland]). Lysed astrocytes were collected into 1.5 mL microcentrifuge tube ...
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bioRxiv - Neuroscience 2024Quote: ... 1x Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Neuroscience 2024Quote: ... 1x Protease Inhibitor Cocktail (Roche)) and incubated for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... 1x Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Neuroscience 2024Quote: ... in a Light Cycler 96 (Roche Diagnostics, Basel, Switzerland), and relative expression levels were calculated using the 2Δ method ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM PMSF and 1x Protease Inhibitor Cocktail (Roche), 100 µg/µl RNaseA) ...
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bioRxiv - Neuroscience 2024Quote: ... as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). The sequencing libraries were multiplexed and clustered onto a flowcell ...
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bioRxiv - Neuroscience 2024Quote: ... 1x Protease Inhibitor Cocktail (Roche)) for 30 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The mixture for qPCR reaction was prepared in a final volume of 20 μl containing 1 μl cDNAs and 10 μl of LightCycler 480 SYBR Green I Master (Roche) in the presence of primers at 500 nM ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then incubated overnight at 4°C in 0.1% goat serum with 0.05% alkaline phosphatase-conjugated DIG antibodies (Roche Cat # 11093274910) and washed before development was carried out using detection buffer containing 20 μL/mL of NBT/BCIP ...
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bioRxiv - Neuroscience 2024Quote: ... 10% DTT) supplemented with Protease and PhosSTOP inhibitor tablets (Roche, USA).
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bioRxiv - Neuroscience 2024Quote: ... each library was amplified for 12 cycles using Kapa HiFi polymerase (Roche, USA), with forward and reverse primers including a 8 nucleotide unique index sequence ...
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bioRxiv - Neuroscience 2024Quote: ... using Fast SYBR Green Master Mix (Roche).
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bioRxiv - Neuroscience 2024Quote: ... and cOmplete Protease Inhibitor Cocktail EDTA-free (Roche) at 4°C ...
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bioRxiv - Physiology 2024Quote: ... 90 & 120 min post-gavage using a glucometer (Accu Check Performa, Roche Diabetes Care). An insulin ELISA was conducted in conjunction with oGTT in the long-term cohort at 46 weeks post-tamoxifen and in the short-term cohort at 18 weeks post-tamoxifen ...
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bioRxiv - Neuroscience 2024Quote: Adenosine 5’-triphosphate (ATP) levels were assessed using an ATP Bioluminescence assay Kit (Sigma/Roche). Briefly ...
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bioRxiv - Neuroscience 2024Quote: The resulting cDNAs were amplified with KAPA HiFi Hotstart Ready Mix (Roche, KK2602) with 0.8 mM cDNA-PCR primer ...
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bioRxiv - Neuroscience 2024Quote: ... we used the KAPA HiFi Hotstart Ready Mix (Roche, KK2602) and a 0.8 μM cDNA-PCR primer to amplify them ...