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Citations for Roche :
2951 - 3000 of 10000+ citations for Progesterone Metabolites ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 0.5% Triton X-100, 1 mM DTT, 150 μM ZnSO4, 1 mM PMSF, 1xEDTA-free protease inhibitors [Roche]) overnight on a rotating wheel at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 million cells were pre-extracted on ice with ice-cold 30 µl CSK buffer (25 mM HEPES pH 7.4, 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 300 mM sucrose, 0.2% Triton X-100, 1 Roche cOmplete protease inhibitor cocktail tablet per 50 ml of buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Remaining red blood cells were resuspended in extraction buffer (50 mM Tris [pH8.0], 1 mM EDTA [pH8.0], 0.5% SDS and 1 mg / ml Proteinase K [Roche, Nutley, NJ]) and incubated overnight at 55°C ...
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bioRxiv - Developmental Biology 2024Quote: ... the sections were washed in a serial SSC buffer and formamide solution and then preincubated in buffer 1 (100 mM Tris-HCl, pH 7.5, 150 mM NaCl) with a 1% blocking reagent (Roche) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated for 1 h at room temperature (20– 25 °C) in 0.1 M PBS with 1% blocking reagent (Roche) and 0.3% Tween 20 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were harvested and washed thrice with FACS washing buffer (1X PBS + 1 mM EDTA, pH 7.2 + 1 Complete Inhibitor EDTA-free tablet (Roche) per 50 mL buffer) ...
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bioRxiv - Immunology 2024Quote: ... followed by tissue digestion for 1 h at 37°C with Iscov’s digestion media containing 1 mg/ml Liberase TL (Roche), and 0.5 mg/ml DNAse (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 50 µg of cryoground tissue powder was placed in a 1.5mL lobind tube and brought to 1 mL with lysis buffer (1% triton, 1x PBS, 1x Roche Complete protease inhibitor cocktail ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 1 ml cytoplasmic lysis buffer (25 mM Tris–HCl pH 7.5, 10 mM NaCl, 1.5 mM MgCl2, 1% IGEPAL CA-630, and 1× protease inhibitor cocktail [“PI”, Roche 11836170001]) at 4°C ...
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bioRxiv - Genomics 2022Quote: ... and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG, RRID:AB_262044 or monoclonal mouse anti-GFP, 1:1,000, Roche #11814460001, RRID:AB_390913) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were prepared by resuspending cell pellets in 2 mL NP-40 buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 1% NP-40, 1 mM PMSF, 1x PhosSTOP [Roche]), sonication on ice (5s on/10s off for 2 mins at 40% amplitude) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was digested for 45-60’ at 37°C in a Thermomixer at 800 rpm in 1 mL of 1× PBS + 0.5 mM CaCl2 + 250 μg/mL Liberase (Roche) + 2 mg/mL of Collagenase (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse atria were minced and digested with mild agitation for 30 minutes at 37°C in DMEM containing 1% P/S and 0.14 Wunsch U ml-1 Liberase (5401119001, Roche). MAFs were enriched via negative selection with magnetic beads against mouse CD45 (leukocytes) ...
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bioRxiv - Cell Biology 2023Quote: 12-16 hours old embryos were collected and lysed in homogenization buffer (25 mM Hepes pH 7.4; 150mM NaCl; 0.5mM EDTA pH 8.0; 1 mM DTT and 1 tablet of proteinase inhibitor cocktail; Roche 11836170001). The aqueous phase of the lysate was collected after 1 hour of centrifugation at 4°C and centrifuged again for 25 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial pellets were solubilized in lysis buffer with 1 % digitonin (25 mM HEPES pH 7.5, 150 mM NaCl, 5 % glycerol, 1 mM TCEP, supplemented with PhosStop (Roche), cOmplete protease inhibitor cocktail EDTA-free (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was washed twice with 300 μl sodium phosphate buffer (20 mM Na-phosphate pH 6.8, 1 mM PMSF, 1× Mini Protease Inhibitor Cocktail, Roche) with centrifugation at 16,000g for 20 min between washes ...
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bioRxiv - Molecular Biology 2023Quote: ... the cell pellet was resuspended in 10 mL of column buffer (20 mM Tris-HCl pH 7.5, 200 mM NaCl, 1 mM EDTA, protease inhibitors [1 Roche Mini EDTA-free tablet] ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in lysis buffer (50 mM PBS pH 7.4, 1 mM EDTA, 5% glycerol, 1 mM PMSF and protease inhibitors from Roche). Cell lysis and protein extraction was achieved through mechanical lysis using glass beads and Precellys®24 Homogenizer (3 cycles of 30 seconds at 5500 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... 140 mM NaC1, 1 mM EDTA, 10% glycerol, 0.1% NP-40, 1 mM PMSF, and 1x protease inhibitor cocktail [Complete; Roche]). The cell pellet was flash frozen in liquid nitrogen with 1.5 mL of lysis buffer and grounded in a Spex Freezer Mill 6775 to a fine powder ...
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bioRxiv - Microbiology 2023Quote: 293T cells transfected with pCAG AcGFP-FOS or pCAG AcGFP-JEVcore-FOS were incubated for 48 h and lysed in lysis buffer (20 mM Tris-HCl [pH = 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche]). The cell lysates were incubated with Strep-Tactin Sepharose (IBM GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in a 1:1 ratio (w/v) in PB supplemented with Complete Protease Inhibitor Cocktail (Roche) and afterward dropwise frozen in liquid nitrogen before lysed in 6875D LARGE FREEZER/MILL (SPEX SamplePrep LLC) ...
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bioRxiv - Immunology 2023Quote: ... were lysed in 1 mL lysis buffer (1% IGEPAL-CA 630, 300 mM NaCl, 100 mM Tris pH 8.0, 1X Roche cOmplete Mini Protease Inhibitor Cocktail ...
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bioRxiv - Plant Biology 2024Quote: ... were ground in liquid N2 and 100 µL extraction buffer added (125 mM Tris-Cl pH 8.8, 1% SDS, 10% glycerol, 1 mM PMSF and Complete Protease Inhibitor [Roche]). Samples were centrifuged at 7000 g for 10 min and supernatant collected ...
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bioRxiv - Plant Biology 2024Quote: ... Root tissues were ground with liquid nitrogen in a mortar before homogenization with the extraction solution (20 mM Tris-HCl, pH 7.0, 1 mM EDTA, 1 mM DTT and a cocktail of protease inhibitors, Roche). The suspension was ultracentrifuged at 105,000 g for 20 min ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates were prepared in a lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, EDTA-free protease inhibitor cocktail [19543200; Roche]). After centrifugation at 17,700 × g for 10 min ...
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bioRxiv - Physiology 2024Quote: ... Pellets were resuspended with binding buffer (100 mM HEPES, 1 mM EDTA, 1% SDS, pH 7.4, and protease inhibitor cocktail from Roche) by vortexing for 20 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were incubated with CENP-B box probe-Cy3 (PNAbio) 1:1 in hybridization buffer (20mM Tris, pH 7.4, 60% formamide, 0.5% of blocking reagent (Roche 11096176001)) ...
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bioRxiv - Immunology 2024Quote: ... Cells were lysed in RIPA buffer (50 mM Tris HCL, 150 mM NaCl, 1% SDS, 0.5% Na-deoxycholate, 1× Complete protease inhibitor (Roche)) for 30 min on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then blocked for 1 h and incubated with an anti-DIG antibody (Roche 11093274910, RRID:AB_514497, 1:2000) diluted in MABT buffer (0.1 M maleic acid pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Single cell suspension was obtained by digesting DRGs in Collagenase type 1 and Dispase II (1 mg/ml; Roche) at 37°C for 70 min18 ...
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bioRxiv - Microbiology 2020Quote: Probes were prepared by PCR amplification using a PCR-DIG labelling kit (Roche) as directed by the manufacturer ...
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bioRxiv - Genomics 2021Quote: ... We made Lhk and CK2ßtes-Y probes using PCR Nick Translation kits (Roche) and ordered oligo probes from IDT ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared using KAPA mRNA (PolyA) HyperPrep Kit (Roche Holding AG, KK8581) using 500 ng RNA per sample according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Viability of the tissue was assessed by an LDH Cytotoxicity Detection Kit (Roche) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... DNA library preparation was performed with the Kapa HyperPrep kit (Roche, Basel, Switzerland), followed by exome target capturing with the SeqCap EZ exome probes V3 kit (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized using the Transcriptor first strand cDNA synthesis kit (Roche, 04379012001). Gene expression levels were analyzed by real-time PCR on an Applied Biosystems model 7500 (software v2.0.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... TUNEL staining was performed using In Situ cell death detection kit (Roche, 11684795910) on coronal cryosections of 12 µm thickness ...
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bioRxiv - Immunology 2021Quote: ... and the final library was quantified using the KAPA Library Quantification Kit (Roche). Pools were sequenced on an Illumina HiSeq 2500 machine using the rapid run setting with single end reads.
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bioRxiv - Genetics 2021Quote: ... PCR products were purified using a High Pure PCR Product Purification Kit (Roche). Customized oligonucleotide primers (S1 Table ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA samples were isolated with an RNA isolation kit (Roche Product No: 11828665001). Purity and concentration measurements were taken with nanodrop at 260-280 nanometers ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was conducted using a SYBR Green Master qPCR Kit (Roche Diagnostics, USA). The relative expression levels were calculated by the ΔΔCT method (Schmittgen & Livak ...
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bioRxiv - Developmental Biology 2021Quote: ... TUNEL was performed using an In Situ Cell Death Detection Kit (Roche, 11684795910). Bodipy-ceramide (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Messenger RNAs were reverse-transcribed with Transcriptor First Strand cDNA Synthesis Kit (Roche). qPCR were performed using the FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... QPCR was performed using LightCycler 480 SYBR Green I Master kit (Roche, Switzerland) on LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2019Quote: ... reverse transcription was performed using the Transcriptor first strand cDNA synthesis kit (Roche) and cDNA analysed on custom real-time ready 384 well custom PCR arrays (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were purified using the High Pure PCR product purification kit (Roche). Libraries were quantified with Qubit high sensitivity assays ...
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bioRxiv - Developmental Biology 2021Quote: ... They were quantified with the KAPA library quantification kit (Kapa Biosystems, MA, USA) according to the manufacturer’s library quantification protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... eurysternus GA72 sample using the Hyper Library construction kit from Kapa Biosystems (Roche). The library was sequenced in a multiplexed mode on one SP lane of NovaSeq 6000 for 251 cycles from both ends of the fragments ...