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Citations for Roche :
251 - 300 of 5196 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and 0.1% deoxycholic acid with protease inhibitors (Roche). The lysates were subjected to anti-GFP immunoprecipitation using GFP- Trap beads (ChromoTek) ...
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bioRxiv - Microbiology 2023Quote: ... or High Pure Viral Nucleic Acid kit (Roche) kits ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated for 50-60 min at 37οC with 20 µM EdU and 100 ng/mL colcemid (Roche). Following metaphase arrest ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 % Triton X-100) with 0.5 mg/ml Lysozyme and 50 μg protease inhibitor cocktail tablet (Roche Biochemicals) followed by sonication on ice ...
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bioRxiv - Genetics 2020Quote: ... with fetal bovine serum (FBS, 10% v/v, Biowest) and Penicillin/Streptomycin (100 U/mL, Roche Diagnostics GmbH). Cells were cultured on 10 cm sterile plates in 37 °C ...
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bioRxiv - Plant Biology 2022Quote: ... incubated with proteinase K (1 mg/mL in 100 mM Tris-HCl, pH 8.0, 50 mM EDTA; Roche) at 37 °C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... then pelleted and resuspended in lysis buffer (50mM HEPES, 150mM NaCl, 0.1% Triton X-100, 0.1 mg/mL Lysozyme, cOmplete Protease Inhibitor (Roche)) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% (v/v) Triton X-100) to leave only matrix before treatment with 10μg/ml DNAse 1 (Roche) to cleave phosphodiester linkages in the DNA backbone ...
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bioRxiv - Microbiology 2021Quote: ... samples were enzymatically analyzed using a D-Lactic Acid/L-Lactic Acid Enzymatic Bioanalysis UV-Test kit and a D-Glucose Enzymatic Bioanalysis UV-Test kit (Roche Diagnostics) with a modified manufacturer’s protocol to reduce total sample size to 300 uL ...
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bioRxiv - Neuroscience 2021Quote: ... 1% Triton X-100 and 0.1% SDS) with a protease inhibitor cocktail (Roche/1 tablet in 10 mL RIPA). Homogenates were centrifuged for 5 min at 4°C at 13,000 x G ...
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bioRxiv - Neuroscience 2021Quote: ... the beads were resuspended in EMSA 1X with 100 U/ml RNAse inhibitor and 1X protease inhibitor cocktail (Roche). FRMP binding assay was performed by incubating biotinylated transcripts (250 ng ...
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bioRxiv - Microbiology 2021Quote: Collected cell pellets were lyzed in a lysis buffer (40 mg/ml SDS, 100 mM Tris-HCl pH 8.6, 10 mM EDTA and Complete protease inhibitors (Roche)) and sonicated 3 times for 30 s at 40% amplitude ...
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bioRxiv - Cell Biology 2020Quote: ... The beads were then resuspended in 100 µl TE plus 0.25% SDS supplemented with proteinase K (500 µg/ml, Roche) and incubated overnight at 60°C ...
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bioRxiv - Cell Biology 2020Quote: ... cell nuclei were counterstained with 100 ng/ml of DAPI for 10 min at room temperature (Roche Applied Science). Slides were mounted on coverslips using Fluoromount mounting medium (DAKO) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then permeabilized with 0.5% TritonX- 100 for 15 minutes at room temperature and incubated with 1mg ml−1 DNase I (Roche) for one hour at 37 °C followed by incubating with anti-BrdU (BU20A ...
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bioRxiv - Immunology 2022Quote: ... Cells were spun down again and the pellet resuspended in 1.25 ml cold lysis buffer (PBS containing 1% TX-100, 1 mM NEM, and Complete Protease Inhibitor from Roche). Cells were lysed for 30 minutes on ice ...
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bioRxiv - Biochemistry 2020Quote: ... 100 mM KCl, 1 mM MgCl2, 0.5 mM β-mercaptoethanol, 0.1 mM GTP, 3 U/ml benzonase, 1X Roche Complete EDTA-free protease inhibitor ...
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bioRxiv - Biophysics 2021Quote: ... Cells were resuspended in 100 ml of lysis buffer (20 mM sodium phosphate pH 7.4, 500 mM NaCl with DNase I (Roche), 0.5 mM MgCl2 and 1 mM PMSF) ...
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bioRxiv - Neuroscience 2022Quote: ... were mixed with an equal volume of 100 mmol/L Tris-HCl (pH 7.4) – 2% sarkosyl for 30 minutes prior to PK (200 μg/mL; Roche) digestion for 1 h at 37° C ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% Triton X-100) supplemented with Complete® protease inhibitor cocktail (Roche, 1 pastel per 10 mL lysis buffer) for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... They were lysed in polysome lysis buffer containing 100 μg/mL cycloheximide and protease cocktail inhibitor EDTA-free (Roche)) ...
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bioRxiv - Biochemistry 2023Quote: ... Pelleted cells were resuspended in 20-30 mL Wash Buffer (150 mM NaCl, 100 mM Tris, pH 8.0) and a cOmplete protease inhibitor tablet (Roche) was added ...
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bioRxiv - Genomics 2023Quote: ... synovial biopsies were minced and digested in a mixed enzymatic-mechanical dissociation including 100 μg/ml Liberase TL (Roche) and 100 μg/ml DNAse I (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining cortical tissue was minced and transferred to 10 mL HBSS containing Collagenase A (Roche, 50-100-3278) at 200 units/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Triton X-100 in PBS for 20 min and followed by 1 hr DNAse (1,000 U/ml; #04536282001, Roche) treatment at 37°C ...
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bioRxiv - Immunology 2023Quote: ... were lysed in 1 mL lysis buffer (1% IGEPAL-CA 630, 300 mM NaCl, 100 mM Tris pH 8.0, 1X Roche cOmplete Mini Protease Inhibitor Cocktail ...
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bioRxiv - Biophysics 2022Quote: ... culture medium was collected and cells were gently washed twice with 5 ml cold PBS and lysed in 0.5 ml of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellet was resuspended in 100 ml of lysis buffer (50mM HepespH8,300mM NaCl,1mM MgCl2, DNase, lysozyme and EDTA-free protease inhibitor cocktails (ROCHE)) at 4 °C and lysed by sonication ...
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bioRxiv - Developmental Biology 2023Quote: ... for nucleic acid hybridization and detection (BBR, Roche, 11096176001) in MAB buffer at RT for 90 min ...
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bioRxiv - Neuroscience 2023Quote: ... using SYBR green as nucleic acid stain (Roche 4913850001). To specifically quantify mRNA expression ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.5% deoxycholic acid containing Complete protease inhibitors (Roche) and PhosStop phosphatase inhibitors (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 % glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was moved to a new 1.5 ml Eppendorf tube and 600 μl of dilution buffer and 100 μl protease inhibitor cocktail (Roche #11836153001) were added ...
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... dried and solubilized in 0.2 ml Hepes (100 mM) buffer containing cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) (1 tablet/20 ml of buffer solution) ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 % glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... Coli tRNA at RT for 10 minutes and resuspended in EMSA 1X with 100 U/ml RNAse inhibitor and 1X protease inhibitor cocktail (Roche). The assay was then performed in presence of 250 ng of biotinylated transcript ...
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bioRxiv - Physiology 2021Quote: ... and 400 ml of sample was placed in a 2-l wide-mouthed Erlenmeyer culture flask with 100 ml of freshly prepared blendzyme (Roche Liberase TM ...
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bioRxiv - Microbiology 2022Quote: PPs were excised from the SI and digested (37 °C, 45 min) using 100 µg/ml liberase TH/DNase (Roche) in RPMI containing 5 % FCS ...
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bioRxiv - Bioengineering 2020Quote: The cell-free reaction mixture for the KGK10 strain is identical to that used for the T7 SHuffle® with the addition of 100 μg/mL DsbC (GeneFrontier) and 3.33 Units/uL T7 RNAP (Roche). Previously prepared KGK10 extract fermented and processed by the Swartz lab was tested alongside the in-house KGK10 extract prepared by shake flasks.
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bioRxiv - Neuroscience 2020Quote: ... To lyse the cells medium was removed and the well was washed with 2 ml ice cold PBS before 100 µl lysis buffer was applied (RIPA buffer supplemented with PhosSTOP (Roche) and protease inhibitors (cOmplete Mini ...
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bioRxiv - Neuroscience 2020Quote: ... 18 µl cleared cell lysate or brain homogenates (20-100 µg based on Tau aggregate content) were incubated with 2 µl 1 mg / ml pronase (Roche) at 37° C for one hour ...
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bioRxiv - Neuroscience 2021Quote: ... medium was removed and the well was washed with 2 ml ice cold PBS before 100 μl lysis buffer were applied (RIPA buffer supplemented with PhosSTOP; Roche) and protease inhibitors (complete Mini ...
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bioRxiv - Microbiology 2021Quote: ... At an OD600 of 5-6 the cells were harvested at 11.325 g and 4 °C for 15 min and cell pellet of 100 ml cell culture was resuspended in 25 ml buffer A (100 mM Tris-HCl, pH 8.0) with cOmplete™ Proteinase inhibitor (Roche). Cell disruption was performed using the French Press cell with a pressure of 172 mPA for five passages ...
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bioRxiv - Plant Biology 2021Quote: ... water was exchanged for 100 µL water with 5 µM L-012 (WAKO chemicals) and 2 µg/mL horseradish peroxidase (Type II, Roche). After the leaf discs were treated with 1 µM 3-OH-C10:0 (stock in MeOH ...
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bioRxiv - Immunology 2023Quote: ... Washed small intestine pieces were then finely chopped and incubated in serum-free medium added with 100 μg/ml Liberase TL (Roche) and 50 μg/ml DNAse I (Roche ...
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bioRxiv - Microbiology 2024Quote: HeLa cells treated with or without CsA were infected with HIV-1 (10 ng p24) that had been treated with 100 U/ml of DNase I (Roche) for 1 h at 37° C ...
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bioRxiv - Neuroscience 2024Quote: Brains were dounce homogenized in RPMI (1X) + 10% Cosmic Calf Serum (Hyclone) and digested with 100 mg/mL collagenase/dispase (Roche) and 1 mg/mL DNase I (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... each supplemented with protease and phosphatase inhibitors (2X cOmplete, EDTA-free, Protease Inhibitor Cocktail [Roche]; 1X PhosSTOP Phosphatase Inhibitor [Roche]; 100 µg/mL Pefabloc SC [Roche]), while incubating on ice for 10 minutes ...
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bioRxiv - Genetics 2022Quote: ... with 5mM EDTA to remove waste and epithelial cells then were minced and dissociated in RPMI containing collagenase (100 mg/ml collagenase A; Roche), DNase I (50 μg/ml ...