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Citations for Roche :
251 - 300 of 355 citations for L Leucine N T Boc H2O 13C6 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Samples were sorted into 100µL of Wash buffer (20mM HEPES pH 7.5, 150mM NaCl, 0.5mM Spermidine, 1x cOmplete Protease Inhibitor Cocktail (Roche)) with 40,000 nuclei per replicate ...
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bioRxiv - Genetics 2019Quote: ... 150 µl of zirconia beads and 150 µl of lysis buffer (50 mM Tris [pH 7.5], 1 mM EDTA, 2.75 mM DTT, 1x PhosSTOP (Roche), 0.48 µg/ml Pefabloc SC (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... Resuspended nuclei in 500μl 1X MNase Buffer (50mM Tris-HCl pH 7.6, 5mM CaCl2, 0.1 mM PMSF, 1X protease inhibitor (Roche)) and incubated them with RNase A at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... which corresponds to approximately 108 colony forming units (CFU)/ml in TSM buffer (2.4 g/L Tris (Roche), 8.77 g/L NaCl (Sigma Aldrich) ...
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bioRxiv - Physiology 2022Quote: ... the muscles were digested in a solution of 10 g/L Collagenase B (11088831001 Roche Canada, Basel, Switzerland) and 4 g/L Dispase II (D4693 Millipore Sigma ...
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bioRxiv - Immunology 2023Quote: ... Blood glucose levels were measured in mice with glycosuria (>110 mmol/L) using Advantage II Glucose strips (Roche). Animals displaying two consecutive blood glucose measurements of ≥ 15mmol/L were considered diabetic.
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bioRxiv - Cell Biology 2023Quote: The experimental cells were collected in a lysis buffer (0.5% SDS, 0.04 mol/L DTT, pH 7.5) supplemented with the protease inhibitor EASYpacks (Roche, Germany). The lysates were diluted with 3 × loading buffer (187.5 mmol/L Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... Lactate was quantified using a lactate assay kit (D-Lactic/L-Lactic acid UV method, r-biopharm, Roche). All samples were measured using fresh growth medium as control.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... n=40) and brain (n=40) for each individual using the KAPA mRNA HyperPrep Kit and Unique Dual-Indexed Adapters (KAPA Biosystems; Wilmington, MA, USA), with 100 ng of large RNA as input ...
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bioRxiv - Microbiology 2019Quote: ... beads containing bound methylated DNA were resuspended in 250 μl digestion buffer (50 mM Tris pH 8, 10 mM EDTA, 0.5% SDS) with 100 μg Proteinase K (Roche) and incubated at 65°C for 4 h ...
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bioRxiv - Biochemistry 2022Quote: ... The cell pellet collected from 6 L growth was resuspended in 20 mM HEPES (pH 7.8) containing protease inhibitor cocktail (Roche) and DNase I (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were resuspended and bead beaten in 250 μL urea buffer (6 M urea, 1% SDS, 50 mM Tris-HCl pH 6.8, 1 mM EDTA, 2x Roche protease inhibitors ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM ATP and 10% glycerol) containing 1 mmol/L PMSF and DNase I (Roche Diagnostics Corp., IN, USA) to 5 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and incubated in buffer A (50 mM MMT pH 8.0/300 mM KCl/10 mM MgCl2/5 % glycerol) containing 1 g L-1 lysozyme/2.5 U mL-1/SmDNAse//complete protease inhibitor cocktail (Roche) for 1 h prior to lysis using EmulsiFlex C5 (Avestin ...
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bioRxiv - Plant Biology 2021Quote: ... and 250 µl extraction buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 % Nonidet P40, proteinase inhibitor cocktail (Roche)) per 200 mg tissue powder was added ...
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bioRxiv - Cell Biology 2021Quote: ... The washed beads were resuspended in 200 μL 50 mM ammonium bicarbonate (pH 8.3) and on-bead trypsin digest (rolling with 1 μg of trypsin (Roche) for 16 h at 37°C followed by a second trypsin digest with 0.5 μg trypsin for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... were mixed with an equal volume of 100 mmol/L Tris-HCl (pH 7.4) – 2% sarkosyl for 30 minutes prior to PK (200 μg/mL; Roche) digestion for 1 h at 37° C ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Molecular Biology 2022Quote: Dried peptides were re-dissolved in 212 µL of pyro-glu buffer (16 mM NaCl, 0.5 mM EDTA, 3 mM cysteamine and 50 μM aprotinin (Roche)) ...
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bioRxiv - Neuroscience 2023Quote: ... Hematocrit (0.5 to 0.64) and blood glucose concentration (5.7 to 8.6 mmol/L, Accu-Chek Performa Nano, Roche, Mannheim, GER) were measured shortly before injecting the radiotracer.
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bioRxiv - Plant Biology 2023Quote: ... in 100μl extraction buffer (Tris-HCl 50 mM pH 6.8, SDS 2%, DTT 2 mM and 1× protease inhibitors (Roche) and centrifuged for 5 min at 13,000g at 4OC ...
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bioRxiv - Molecular Biology 2023Quote: 3-5 day old males of the indicated genotypes and experimental groups were homogenised in 150µl of homogenisation buffer (0.2%Triton X-100 supplemented with 1 x complete mini EDTA-free protease inhibitor (Roche) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μl of the sample were treated for 30 min at 50°C with 1.2 mg/ml Proteinase K (Roche) in Proteinase K Buffer (100 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was washed twice with 300 μl sodium phosphate buffer (20 mM Na-phosphate pH 6.8, 1 mM PMSF, 1× Mini Protease Inhibitor Cocktail, Roche) with centrifugation at 16,000g for 20 min between washes ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed in 100μL or 200μl of 2% SDS lysis buffer (2% SDS, 50mM Tris-HCl pH 7.5, 5mM EDTA, 15U/mL DnaseI (Roche), cOmplete mini EDTA-free protease inhibitor tablet (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume). The suspension was frozen and stored at −80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume) were added to the cell suspension and stirred at RT for 30 mins ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached using cold PBS and lysed in 350 µl lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor (Roche) for at least 20 min on ice ...
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bioRxiv - Neuroscience 2024Quote: ... underwent lysis through the addition of 200 µl of a 50 mM Tris-HCl buffer (pH 7.8) containing 5% SDS and cOmplete ULTRA protease inhibitor (Roche). The samples were then subjected to Bioruptor® treatment (Diagenode ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% BSA/TBS-T) at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with 800 ng of plasmid vectors (50 ng / 750 ng or 200 ng / 600 ng of pAAV-Syn-eGFP-2A-mCherry_D4H/YDA or pAAV-Syn-Myr_mCherry-2A-eGFP and pAAV-iCre control respectively) using the FuGene6 transfection protocol (Roche, cat n°11 844 443 001) at a 3:1 DNA:FuGene ratio ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5% Triton X-100, 10 mM N-methyl maleimide (general DUB inhibitor diluted in DMSO, freshly added) and protease inhibitors (Roche Diagnostics, EDTA-free, freshly added). Then ...
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bioRxiv - Molecular Biology 2020Quote: Frozen extracted mitochondria were lysed by sonication in 300 μl of lysis buffer (8 M urea, 50 mM ammonium bicarbonate, 1X protease inhibitors (Complete mini EDTA-free mixture [Roche Applied Science] ...
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bioRxiv - Neuroscience 2021Quote: ... and resuspended in 25μL ice cold ATAC-RSB buffer (10mM Tris-HCl, pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% NP40 (Roche cat#11332473001)) ...
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bioRxiv - Molecular Biology 2020Quote: ... In a 50 μl-reaction the concentrated DNA (15 μl) was mixed with 10 μl of the Nextera index mix (i5 + i7) and 2x Kapa HiFi Master Mix (Roche). The PCR was performed in a Thermocycler (Eppendorf mastercycler ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were then pelleted again and resuspended in 150 μL of Lysozyme buffer (150 mM NaCl, 30 mM Tris–HCl pH 8, 10 mM EDTA, cOmplete protease inhibitor (Roche), 1 mg/ml lysozyme ...
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bioRxiv - Physiology 2021Quote: ... and 400 ml of sample was placed in a 2-l wide-mouthed Erlenmeyer culture flask with 100 ml of freshly prepared blendzyme (Roche Liberase TM ...
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bioRxiv - Plant Biology 2021Quote: ... The construct was used to transform wild type plants following the standard floral dip method (Clough and Bent, 1998) and selected with 50 mg L-1 Hygromycin (Roche). A total of 60 T1 plants were individually genotyped by PCR and sequencing ...
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bioRxiv - Cancer Biology 2021Quote: 160 μl DMEM containing 10 % FBS were added to the bottom wells of xCELLigence Real-Time Cell Analyzer CIM plate (Roche), as chemo-attractant ...
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bioRxiv - Neuroscience 2019Quote: DIV14-15 cortical neurons (250.000 neurons/mL in laminin/poly-L-ornithine coated 6-well plates) were washed and scraped with ice cold PBS with protease inhibitor cocktail (Roche). Neurons were pelleted (5 minutes at 3000 g at 4°C) ...
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bioRxiv - Genomics 2019Quote: ... The dry contents were resuspended by addition of 7.5 µl hybridization buffer and 3 µl hybridization component A (SeqCap EZ Hybridization and Wash Kit: 05 634 261 001, Roche), mixed by tapping ...
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bioRxiv - Microbiology 2019Quote: ... cellular pellets were suspended in 10 mL of Buffer 1X supplemented with 2 mM (L+D) 1,4-Dithiothreitol (DTT) and cOmplete™ mini protease inhibitor cocktail (Roche). To avoid overheating and protein denaturation ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 mL of Sf9 cells at a density of 1.2×106 cells/mL were transfected with 5 µL of recombinant bacmid containing the gene of interest using 3 µL of X-tremeGENE HP DNA transfection reagent (Roche) and 100 µL of transfection medium (Expression Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.78 g/L CSM-His and 20 g/L agar) and screened for expression of Erg6-GFP by Western blot with anti-GFP bodies (11814460001; Roche) and for localization of Erg6-GFP to LDs by fluorescence microscopy.
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bioRxiv - Developmental Biology 2021Quote: ... brn3a1 and brn3a2(l) transcripts were visualized with anti digoxigenin-alkaline phosphatase conjugates and BCIP/NBT or BM purple substrates (Roche). For two color WISH krx-20 (Oxytoby and Jowett ...
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bioRxiv - Genetics 2021Quote: ... The cultures were spun down and cell pellets were resuspended in 500 μL of Workman Extract Buffer (40mM HEPEs pH7.4, 250mM NaCl, 0.1% NP40, 10% Glycerol, 1 mM PMSF, Roche proteinase inhibitors). 250 μL of glass beads were added and cells were lysed with Peqlab precellys homogenizator (3×30 sec) ...
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bioRxiv - Plant Biology 2021Quote: ... Two µL of diluted cDNA was used as a template for the qRT-PCR with Fast SYBR Green Master Mix (Roche) on a Light-Cycler 96 instrument (Roche ...
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bioRxiv - Immunology 2020Quote: ... The mucosa was stripped and dissociated in GentleMACS tubes in digestion medium (DM; complete medium (RPMI 1640 with PGA/L-glutamine/10% FCS) with 1 mg/mL Collagenase D (Roche), 1 mg/mL soybean Trypsin inhibitor (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... water was exchanged for 100 µL water with 5 µM L-012 (WAKO chemicals) and 2 µg/mL horseradish peroxidase (Type II, Roche). After the leaf discs were treated with 1 µM 3-OH-C10:0 (stock in MeOH ...
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bioRxiv - Cancer Biology 2022Quote: 1 μL amplified cDNA library was used as template in a 29-cycle PCR reaction using KAPA HiFi HotStart ReadyMix (Roche). To avoid possible PCR-induced library biases ...