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Citations for Roche :
251 - 300 of 335 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: TUNEL assay [47] was performed using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
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bioRxiv - Developmental Biology 2023Quote: ... Livers were then dissected out of the larvae and subjected to TUNEL assay according to manufacturer’s instructions (In Situ Cell Death Detection kit, Fluorescein; Roche). Nuclei were counterstained with Hoechst (Thermo Fisher Scientific ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... 100mM sodium chloride) for at least 2 hours and then incubated in anti-fluorescein-POD Fab fragments (Roche, 11207733910) diluted at 1:400 overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA fragmentation was visualized by using an in situ cell death detection kit (Fluorescein; Cat #, 11684795910; Roche, Basel, Switzerland) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... followed by transcription with T7 RNA polymerase and a nucleotide mixture containing digoxigenin-labeled or fluorescein-labeled UTP (Roche), resuspended in formamide and examined by agarose gel electrophoresis.
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bioRxiv - Biophysics 2023Quote: ... 50 mM KCH3COO, 2 mM MgSO4, 1 mM EGTA, 5% glycerol, 0.2mM Mg-ATP, 0.1% Octylglucoside, 0.5mM DTT, Roche cOmplete™ Protease Inhibitor Cocktail EDTA free) ...
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bioRxiv - Cell Biology 2022Quote: ... For LC-MS/MS based experiments the kinase assay buffer contained 80 μM cold ATP (10519979001, Roche)) ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described (Pearson et al., 2009) and detected with anti-digoxigenin-HRP (1:2000, Roche/Sigma-Aldrich 11207733910 ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA-probe hybrids were detected by an alkaline phosphatase-conjugated antibody (Anti-Digoxigenin-AP or Anti-Fluorescein-AP, Fab Fragments, 1:5000, Roche, Cat# 11093274910 ...
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bioRxiv - Bioengineering 2021Quote: ... the probes were detected with anti-digoxigenin and anti-fluorescein antibodies conjugated with alkaline phosphatase (AP) and horseradish peroxidase (HRP) using AP detection kit (Roche) and tyramide signal amplification (TSA ...
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bioRxiv - Developmental Biology 2022Quote: ... Riboprobes labeled with digoxigenin (DIG), fluorescein (FLU) or dinitrophenol (DNP) were prepared as described (Opitz et al., 2011) using DIG RNA labeling kit (Roche), FLU RNA labeling kit (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... high specific-activity RNA probes were produced from a linearized plasmid (HindIII) and labeled using the Fluorescein RNA Labeling Mix (Roche), following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... and incubated with α-DIG or α-fluorescein antibody conjugated with alkaline phosphatase (Roche, 1:2000 in 10% FBS/PBST) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (Gavrieli, Sherman, and Ben-Sasson 1992) was performed using the fluorescein kit from Roche Diagnostics (Mannheim ...
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bioRxiv - Neuroscience 2021Quote: Digoxigenin RNA-labeled or Fluorescein RNA-labeled probes were transcribed in vitro using the RNA Labeling Kit (Roche Diagnostics Corporation) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cell nuclei with DNA strand breaks at the early stage of apoptosis were labeled using fluorescein-dUTP in the TUNEL reaction (In Situ Cell Death Detection Kit; Roche) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Alkaline hydrolysis was then performed at 60°C for 30 min to fragment the target RNA before labeling it with Digoxigenin and Fluorescein according to kit instructions (Roche).
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bioRxiv - Neuroscience 2021Quote: ... blocked with blocking solution for 1 hour and finally incubated overnight at 4°C with anti-Fluorescein-POD antibody (Roche) diluted 1:500 in blocking solution ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... and in vitro transcribed using T7 (pSC-pbx3a, pSC-cdx1b) or T3 polymerase (pSC-best4, pSC-otop2) and fluorescein or digoxygenin RNA labeling kits (digoxygenin: Roche, Cat# 11277073910 ...
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bioRxiv - Developmental Biology 2023Quote: ... Resulting amplicons were used as templates for the synthesis of either digoxigenin (DIG) or fluorescein (FITC)-labelled anti-sense probes by in vitro transcription using T7 or T3 polymerases (Roche) and DIG or FITC RNA labelling mixes (Roche).
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining on 60 dpf paraffin embedded testes sections were performed using a fluorescein In Situ Cell Death Detection Kit (11684795910, Roche). Immunolabelling with Vasa antibody was done before the TUNEL staining ...
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bioRxiv - Pathology 2024Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche Diagnostics GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... in the presence of ATP regeneration system (3.5 U/mL creatine kinase [Roche], 3.8 mM creatine phosphate disodium [Roche] ...
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bioRxiv - Cancer Biology 2023Quote: ... In vitro translation reactions with luciferase reporter mRNAs were carried out with 0.5x extract, energy mix (final 0.84 mM ATP, 0.21 mM GTP, 21 mM creatine phosphate (Roche 10621722001), 45 U/mL creatine phosphokinase (Roche 10127566001) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.25% CHAPS, 5 mM ATP, 5 mM MgCl2, 0.25 mM EDTA, 2 mM DTT, 10% glycerol, 1× Roche cOmplete protease inhibitors ...
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bioRxiv - Developmental Biology 2021Quote: ... 2009) and detected with anti-digoxigenin-HRP (1:2000, Roche/Sigma-Aldrich 11207733910, lot 10520200 anti-fluorescein-HRP (1:2000, Roche/Sigma-Aldrich 11426346910 ...
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bioRxiv - Neuroscience 2020Quote: TUNEL staining was performed according to the manufacturer’s instructions (In Situ Cell Death Detection Kit, Fluorescein, Roche, #11 684 795 910). In brief ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... assay [31] was performed on cryosections of retinal explants using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
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bioRxiv - Plant Biology 2020Quote: ... were labelled by PCR in the presence of digoxygenin-11-dUTP detected by antidigoxigenin-fluorescein isothiocyanate (FITC; both from Roche Diagnostics) and biotin-16-dUTP (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell death was determined by the TUNEL assay using the In Situ Cell Death Detection Kit Fluorescein (Cat # 11684795910; Roche, Germany); only a signal overlapping a DAPI-stained nucleus was considered positive ...
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bioRxiv - Genomics 2023Quote: ... was indirectly labeled by PCR in the presence of digoxigenin-11-dUTP detected by antidigoxigenin-fluorescein isothiocyanate (FITC; both from Roche Diagnostics). The remaining satDNAs (‘pTS5’ ...
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bioRxiv - Plant Biology 2022Quote: ... Hybridization signals of probes labelled with digoxigenin-11-dUTP and biotin-16-dUTP were detected with antidigoxigenin conjugated to fluorescein isothiocyanate (FITC; Roche Diagnostics) and streptavidin conjugated to Alexa 594 or Alexa 647 (Life Technologies-Molecular Probes) ...
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bioRxiv - Neuroscience 2023Quote: ... DNA templates for Tac2 and Agrp were subjected to in vitro transcription with DIG (cat#11277073910)- and fluorescein (cat#11685619910)-RNA labeling mix and T3 RNA polymerase (cat#11031163001) according to the manufacturer’s instructions (Roche Applied Science).
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bioRxiv - Molecular Biology 2023Quote: ... and fluorescein (FITC)-labeled riboprobes were synthesized using T7 or SP6 polymerase along with a DIG or FITC labeling mix (Roche Diagnostics). For the detection of hybridization signals ...
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bioRxiv - Neuroscience 2022Quote: ... for 18 hours at 55°C and incubated with different combinations of anti-fluorescein isothiocyanate (FITC)- and anti-digoxigenin (DIG)-conjugated antibodies (Roche Diagnostics), followed by tetramethylrhodamine (TAMRA)-(Thermofisher ...
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bioRxiv - Plant Biology 2024Quote: ... The probe pXV1 [31] for the 5S rRNA gene was labeled with digoxygenin-11-dUTP by PCR and detected by anti-digoxigenin–fluorescein isothiocyanate (FITC; both from Roche Diagnostics). The hybridization procedure was performed as described previously [31] ...
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bioRxiv - Developmental Biology 2024Quote: RNA probe was produced from plasmid or PCR product template by in vitro transcription using Digoxigenin/Fluorescein (Roche, Cat. 11277073910/11685619910) uridine-5’-triphosphate (UTP ...
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bioRxiv - Molecular Biology 2021Quote: ... in a total volume of 40 µl to 2.4 pmole trypSL in the presence of 1 mM ATP and 20 U RNasin (Roche) in vaccinia capping buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM ATP and 10% glycerol) containing 1 mmol/L PMSF and DNase I (Roche Diagnostics Corp., IN, USA) to 5 µg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 10 ml buffer A (50 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM MgCl2, 5 mM ATP, Roche cOmplete EDTA-free protease inhibitor catalog # 11872580001) ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5, 150 mM NaCl, 5 mM EDTA, 2 mM ATP, 1 mM dithiothreitol and protease inhibitor cocktail tablets; Roche) using Bead Beater ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... resuspended in 10 ml buffer A (50 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM MgCl2, 5 mM ATP, Roche complete EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in lysis buffer (300 mM KCl, 1 mM MgCl2, 0.1 mM ATP, 1X Roche Protease/PPase inhibitor ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of 180 mM of adenosine triphosphate (ATP) and 0.5 U of Glucose-6-phosphate dehydrogenase(G6PDH) (Roche). After the absorbance at 340 nm was recorded for 20 min to determine a baseline ...
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bioRxiv - Pathology 2022Quote: We detected DNA fragmentation resulting from apoptotic signaling cascades with the In Situ Cell Death Detection Kit Fluorescein (Roche, 11684795910, Basel, Switzerland) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Fluorescently labeled RNA was produced by transcribing pSP72 DNA cut with EcoRI in vitro using T7 RNA polymerase with a nucleotide mixture containing fluorescein-12-UTP (Roche Applied Science). Steady-state rotational anisotropy reactions (60 μL ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-sense riboprobes against target genes were synthesized from 5 µg linearized plasmids using digoxigenin-(DIG) or fluorescein-labeled uridylyltransferase (UTP) (#11685619910, #11277073910, Roche, Mannheim, Germany) and the MAXIscript in vitro Transcription Kit (#AM1312 ...
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bioRxiv - Cell Biology 2021Quote: ... apoptotic cells in pancreas frozen sections were detected by the TUNEL (TdT mediated dUTP nick end labeling) assay using a fluorescein-based detection kit (in situ death detection kit, Roche, Indianapolis, IN) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... The PCR products of target genes were purified and used as templates for probe preparation using DIG or fluorescein RNA labeling kits (Roche, Basel, Switzerland). The pituitary sections were hybridized with the antisense RNA probes for fshb and lhb as we previously reported [52] ...
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bioRxiv - Neuroscience 2024Quote: ... Antisense riboprobes against target genes were synthesized from 5 μg linearized plasmids using digoxigenin-(DIG) or fluorescein-labeled uridylyltransferase (UTP) (#11685619910, #11277073910, Roche, Mannheim, Germany) and the MAXIscript in vitro Transcription Kit (#AM1312 ...