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Citations for Roche :
251 - 300 of 860 citations for 3 22 Dihydroxyolean 12 En 29 Oic Acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: To prepare the RNA:DNA identifier we combined 12 µL of 3,569 nt long MS2 RNA (Roche; 30 nM) with 2.4 µL of complementary oligonucleotides each at a concentration of 1 µM (IDT) ...
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bioRxiv - Neuroscience 2021Quote: ... hydrated through an alcohol series and boiled in CC1 (citric acid buffer, Roche Diagnostics, Basel Switzerland) for 1 hour (pH 6 ...
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bioRxiv - Genomics 2020Quote: ... concentrators and purified in large volume columns (High Pure Viral Nucleic Acid Large Volume Kit, Roche) using 10X (2.5 ml ...
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bioRxiv - Microbiology 2020Quote: ... or the MagNA Pure LC Total Nucleic Acid Isolation Kit (Cat. 03038505001, Roche Diagnostic, Basel, Switzerland) following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2021Quote: ... as described by (18)) with supplemented ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail tablets (Roche Diagnostics), 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acid extraction was performed using the MagNA Pure Compact DNA Isolation Kit I (Roche Diagnostics) on the MagNA Pure LC automated extractor ...
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bioRxiv - Microbiology 2021Quote: ... Total nucleic acid was extracted from samples with the MagNA Pure 96 system (Hoffmann-La Roche) using the MagNA Pure 96 DNA and Viral NA Small Volume Kit and eluted in a volume of 50μl Roche Tris-HCl elution buffer ...
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bioRxiv - Immunology 2020Quote: ... viral RNA was isolated from plasma using a MagNA PureCompact Nucleic Acid Isolation kit (Roche Diagnostics). Real-time RT-PCR was performed using a QuantiTec Probe RT-PCR kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... Viral DNA was then extracted using the High Pure Viral Nucleic Acid Kit (Roche Applied Science) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... 2% Triton X-100) containing ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Roche cOmplete, Sigma Aldrich)] ...
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bioRxiv - Microbiology 2023Quote: Nucleic acids were purified from whole blood or serum using the MagNA Pure 96 system (Roche) with the DNA/Viral NA 2.0 kit and the Viral NA Plasma external lysis S.V ...
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bioRxiv - Microbiology 2024Quote: ... and viral genomes were purified with the High Pure Viral Nucleic Acid kit (Roche, Mannheim, Germany). Genome quantification of DENV ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 tetrazolium]-bis(4-methoxy-6-nitro)-benzene sulfonic acid hydrate (XTT)-based colorimetric assay (Roche) according to manufacturer-recommended protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... The genomic DNA was extracted from pools using High Pure Viral Nucleic Acid Kit (Roche, Germany) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... 3 mM phenylmethanesulphonylfluoride (PMSF) and complete Mini protease inhibitor cocktail (Roche). Following lysis ...
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bioRxiv - Neuroscience 2021Quote: ... and 5’-GCTTGAGGTAGC CCTGTTGTCACC-3’ using KAPA HiFi Hotstart polymerase (Roche:KK2602). This PCR reaction produced a 185 bp wild type band and a 750 bp knockout band in heterozygous animals ...
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bioRxiv - Cell Biology 2022Quote: ... DNA (1 μg) was mixed with 3 μl Fugene6 (Roche, #11836145001) in 200 μl opti-MEM (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... then equilibrated for 3 min in detection buffer (Roche, Catalog# 11585762001). Signals were visualized with CDP-Star Ready-to-Use (Roche ...
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bioRxiv - Microbiology 2020Quote: ... and BCIP (1,5-bromo-4-chlooro-3-indolyl-phosphate, Roche, Switzerland).
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bioRxiv - Developmental Biology 2022Quote: ... and 0.035 mg/ml 5-bromo-4-chloro-3-indolyphosphatase (Roche). Images were acquired by the Leica MZ10F microscope with the DS-Ri1 camera.
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bioRxiv - Physiology 2022Quote: ... pH 7.4) containing 3 mg/mL collagenase A (Roche Diagnostics, Germany). Oocytes of stage IV and V were manually defolliculated and each oocyte was injected with 50-100 ng of mRNA encoding for the respective photoreceptor CNG channels ...
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bioRxiv - Neuroscience 2023Quote: ... with 3 mg/mL DNAse I grade II (Roche, Cat# 104159). The cell suspension was centrifuged at 1,300 rpm for 5 minutes with 7.5% BSA solution (Sigma ...
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bioRxiv - Immunology 2024Quote: ... in HEPES buffer containing Liberase Blendzyme 3 (70 mg/ml; Roche) and DNaseI (30 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP; 11383221001, Roche) was performed in alkaline phosphatase reaction buffer [100 mM Tris pH 9.5 ...
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bioRxiv - Cell Biology 2023Quote: MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Roche) was performed following manufacturer’s indications ...
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bioRxiv - Developmental Biology 2024Quote: ... 5’-AGCGTTCACATCATATGGCA-3’) using Taq DNA polymerase (Roche, Cat. No. 11146165001). Fragments of foxl2l and id1 were cloned into pGEMT-easy plasmid by TA cloning while nanos2 fragment was cloned into pCS2+ plasmid by BamHI and KpnI ...
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bioRxiv - Neuroscience 2023Quote: ... and BCIP (5-bromo-4-chloro-3-indolyl-phosphate, Roche 11383221001). Larvae were mounted in 100% glycerol under coverslips and bright-field images captured using a Leica DFC500 camera mounted on a Zeiss Axioskop
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bioRxiv - Biochemistry 2023Quote: ... and 3 cOmpleteTM mini EDTA-free Protease Inhibitor cocktail tablets (Roche)) ...
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bioRxiv - Immunology 2023Quote: ... Fat was digested enzymatically with 3 mg/ml collagenase/dispase (Roche) at 37°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with 3 U PNGase F (Roche diagnostics) at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were injected with diazepam (3 mg/kg, Roche Pharmaceuticals, CH), gaboxadol (10 mg/kg ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mM CaCl2 and protease inhibitor cocktail (CompleteTM EDTA-free, Roche), and homogenized by 10 strokes using Dounce device ...
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bioRxiv - Developmental Biology 2024Quote: ... Nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (Roche) and Fast Red (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 3 mM CaCl2 and protease inhibitor cocktail (CompleteTM EDTA-free, Roche). After centrifugation at 400g for 5 min at 40C ...
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bioRxiv - Cell Biology 2021Quote: ... A cDNA library was made from Oligo(dT)12–18 as described in the manufacturer’s protocol (Transcriptor First Strand cDNA Synthesis Kit, Roche). Quantitative PCR was performed using Quantifast SYBR Green PCR Kit (204052 ...
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bioRxiv - Microbiology 2020Quote: ... Reaction mixtures for each sample (24 µl) included 12 µl KAPA HiFi HotStart ReadyMix (2X) (KAPA Biosystems, Boston, Massachusetts), 1.5 µl NanoPure water ...
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bioRxiv - Cell Biology 2021Quote: ... were transfected into COS-7 cells (14,000-17,000 cells/cm2 in 12-well plate) for 24 h using X-tremeGENE HP DNA (Roche, #06366236001). Cells were incubated for 7 min at 37 °C with either 4 μg.mL-1 rabbit anti-γ2 Ex2 or 1:50 serum rabbit anti-γ8 Ex1 antibodies prior to fixation ...
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bioRxiv - Cell Biology 2020Quote: ... 50 ng of nick translated probe per coverslip was combined with 12 μg of human Cot-1 DNA (Roche), 10 μg salmon sperm ssDNA (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... containing 25 Abs260 ribosome units (1 A260 = 12 μg/μL) [5] were digested with 60 U of MNase (Roche) and 60 U of SUPERase·In (Ambion ...
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bioRxiv - Cancer Biology 2023Quote: ... The medium from each well was removed and replaced with fresh medium containing 10 µl of 12 mM MTT as per the manufacturer’s instruction (Roche). After 4 h of incubation ...
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bioRxiv - Plant Biology 2024Quote: ... on a rocker with horseradish peroxidase (HRP)-conjugated HA antibody (Roche, Indiana, USA, Cat. No. 12 013 819 001). Membranes were washed three times in 1X Tris-Buffered Saline (TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% blocking buffer pH 7.5 (100 mM maleic acid, 150 mM NaCl, 10% Roche blocking reagent #11096176001). Antibody staining was performed in 3% BSA in PBS-Triton (0.1%) ...
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bioRxiv - Systems Biology 2021Quote: ... After neutralizing the samples with HCl the free fatty acids were assayed using a commercial kit (Roche). Concentrations of cholesterol and acetate were measured using commercial kits supplied by Boehringer Mannheim.
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bioRxiv - Microbiology 2020Quote: Automated nucleic acid extraction was performed on the MagNA Pure 96 instrument (Roche Applied Science, the Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... washed for 30 min with washing buffer (1 × maleic acid buffer + 0.3% Tween 20; Roche, Catalog# 11585762001), then equilibrated for 3 min in detection buffer (Roche ...
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bioRxiv - Microbiology 2020Quote: ... GISAID: EPI_ISL_413570) obtained from cell culture using the MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche). We determined the slope by linear regression in GraphPad Prism and defined the required levels for PCR efficiency (E ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted using the column-based High Pure Viral Nucleic Acid Kit (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... liposomes (final lipid concentration = 1 mM) were blocked with 4% (w/v) fatty-acid-free BSA (Roche) in HK buffer for one hour at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... 40% isopropanol) and DNA was purified using a High Pure Viral Nucleic Acid kit (Roche Life Science) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Fatty acid-free bovine serum albumin (BSA) and Complete Protease Inhibitor cocktail tablets were procured from Roche Diagnostics (Laval ...