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Citations for Roche :
251 - 300 of 2703 citations for 2S 4S 4 Fmoc amino pyrrolidine 2 carboxylic acid methylester hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... were ground to fine powder in liquid nitrogen and homogenized in 575 μL of lysis buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.15% IGEPAL (CA-630, Merk) and 1x cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Microbiology 2021Quote: ... cell extracts were lysed in TNN lysis buffer (100mM Tri-HCl pH8.0, 250 mM NaCl, 0.5% NP-40, and cOmplete protease inhibitor (Roche)) and centrifuged for 7 min at 15’000 rpm and 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Pellet was resuspended in 1mL of isolation buffer (225mM mannitol, 75mM sucrose, 30mM Tris-HCl pH 7.4, 0.1mM EGTA and protease inhibitors (Roche), and homogenized with a 25G needle at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with RIPA buffer (25 mM Tris HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche]). The lysates were subjected to SDS-PAGE under denaturing conditions in 12% polyacrylamide gels using Bio-Rad Mini PROTEAN electrophoresis system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: Cells transfected with indicated constructs were harvested and lysed in NETN buffer (10mM Tris-HCl [pH 8.0], 100mM NaCl, 1mM EDTA, and 0.5% NP-40) plus protease inhibitor (Roche) on ice for 30 min ...
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bioRxiv - Genomics 2022Quote: ... twice 10 min with cold TET buffer (10 mM Tris/HCl pH7.5, 1 mM EDTA, 0.1% Tween-20, protease inhibitor cocktail (Roche)) and eluted with TE buffer ...
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bioRxiv - Immunology 2020Quote: ... 10 million cells were harvested and lysed in EBC buffer (50 mM Tris-HCl pH 7.5, 120 mM NaCl, 0.5% NP40) with protease inhibitors (Complete Mini, Roche) for 30 min on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were lysed with NETN-300 buffer (20mM Tris-HCl Ph8.0, 300mM NaCl, 1mM EDTA, 0.5% Nonidet P-40) containing protease inhibitor cocktail (Roche), 3μM TSA and 50mM NAM on ice for 10min ...
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bioRxiv - Genomics 2020Quote: ... and resuspended in 1mL per 50E6 nuclei of Tep20 Buffer (10mM Tris-HCl pH 7.5, 0.1mM EGTA, 20mM NaCl, and 1X Protease Inhibitor (Roche) added immediately before use ...
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bioRxiv - Microbiology 2022Quote: ... cell extracts were lysed in TNN lysis buffer (100mM Tri-HCl pH8.0, 250 mM NaCl, 0.5% NP-40, and cOmplete protease inhibitor (Roche)) and centrifuged for 7 min at 15’000 rpm and 4°C ...
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bioRxiv - Microbiology 2022Quote: ... were incubated at 4°C with hypotonic lysis buffer (10 mM Tris–HCl pH 8.0, 1 mM KCl, 1.5 mM MgCl2, with protease inhibitors (ROCHE)) for 2 hours while rotating ...
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bioRxiv - Microbiology 2021Quote: ... 1.1% Triton X-100, 1.2 mM EDTA, 20 mM Tris-HCl pH8.0, 167 mM NaCl, complete protease inhibitor [Roche]). Antibody (2 μg ...
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bioRxiv - Microbiology 2021Quote: ... 1.1% Triton X-100, 1.2 mM EDTA, 20 mM Tris-HCl PH8.0, 500 mM NaCl, complete protease inhibitor [Roche]), and LiCl (250 mM LiCl ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet was suspended in 1 mL of cold lysis buffer (50 mM TRIS-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2021Quote: Protein lysates were prepared from snap-frozen dorsal skin with urea buffer (8 M Urea, 40 mM Tris-HCl, 2.5 mM EDTA, pH 8.0) containing phosphatase (PhosSTOP, Roche) and protease inhibitors (cOmplete Protease Inhibitor Cocktail Tablet EDTA-free ...
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bioRxiv - Microbiology 2020Quote: ... and lysed by resuspension in lysis buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 1 mM EDTA, 0.5% NP-40, and protease inhibitor [Roche]) with rotation at 4°C for 30 min ...
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bioRxiv - Genomics 2021Quote: ... Pellets were resuspended in 500 μL cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP-40, 1X protease inhibitor (Roche)) and rotated for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... washed twice with PBS and lysed in 5 ml lysis buffer (50 mM Tris-HCL, ph7,4; 500 mM NaCl, 0,2% SDS; 1x protease inhibitors (Roche), 20 mM DTT ...
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bioRxiv - Molecular Biology 2020Quote: ... washed twice with 1x PBS and resuspended in cold NP40 lysis buffer (10mM Tris-HCl pH 7.4; 10mM NaCl; 3mM MgCl2; 0.5% NP40 with freshly added protease inhibitors (Roche)) ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 20 mM Tris-HCl pH 8 containing a EDTA-free protease inhibitor cocktail (Roche). For the purification of protein samples that had to be analyzed both by reducing and non-reducing SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... and then lysed and harvested in ChIP lysis buffer (50 mM Tris-HCl pH 8.1; 0.5% SDS; 10 mm EDTA;100 mM NaCl, 1mM PMSF, Proteinase inhibitor Roche) by centrifugation for 6 min at 2,000 × g ...
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bioRxiv - Synthetic Biology 2023Quote: ... resuspended in 500 μL of Buffer B (30 mM Tris-HCl pH 7.9, 0.5 mM EDTA, antiprotease complete tablets (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysis buffer (50 mM Tris-HCl, pH 7.5; 500 mM NaCl; 10 mM imidazole; 0.5 mM TCEP; 1X Roche cOmplete protease inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... and pellets resuspended in lysis buffer (50 mM Tris-HCl, pH 7.5; 150 mM NaCl; 0.5 mM TCEP, 1X Roche cOmplete protease inhibitors ...
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bioRxiv - Plant Biology 2023Quote: Total proteins were extracted from 6 g of frozen ground Arabidopsis seedling tissue with IP buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% NP-40, 1% Triton, EDTA-free protease inhibitor cocktail [Roche]). Lysates were cleared by centrifugation (6,000 g ...
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bioRxiv - Molecular Biology 2023Quote: ... A 2L spinner culture of asynchronous HeLa S3 cells was collected and homogenized using nitrogen cavitation in swelling buffer (20mM Tris- HCl [pH 7.5], 5mM KCl, 1.5mM MgCl2, 1mM DTT, 1x protease-inhibitor tablet(Roche)) ...
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bioRxiv - Molecular Biology 2022Quote: ... After exchanging the buffer with 100μl PI-lysis buffer (50mM Tris-HCl [pH7.5], 500mM NaCl, 2mM EDTA, 2mM DTT, 0.4%[w/v] SDS, cOmplete Protease Inhibitor Cocktail Tablet [Roche]), testes were homogenized using Bioruptor (Diagenode ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were lysed with 5mL cold lysis buffer (10 mM Tris-HCl, pH 7.5; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; 1x complete protease inhibitor; 11836145001 Roche) for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed 2x with ice-cold PBS and then incubated for 10 min on ice with swelling buffer (10mM Tris-HCl [pH 7.5], 10% glycerol, 3mM CaCl2, 2mM, MgCl22, 0.5mM DTT, protease inhibitors [Roche] and 4 U/ml RNase inhibitors [RNaseOUT ...
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bioRxiv - Cell Biology 2022Quote: ... the chromatin pellet was suspended with buffer B (20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 10 mM EDTA, and 1× complete protease inhibitor cocktail; Roche), and then mononucleosome was extracted ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated by lysing cells (10 mM Tris-HCl pH 8.0, 1 mM EDTA, 0.67% SDS, and 125 μg/ml proteinase K from Roche), followed by incubation 4 hours at 55°C with weak agitation ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with PBS and lysed for 20 min on ice in lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, supplemented with protease inhibitors [Roche]). Centrifugation at 13,000×g for 10 min at 4°C was then used to remove cell debris ...
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bioRxiv - Genomics 2024Quote: ... Cell pellets were resuspended in 250 µL ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% Igepal CA630, 1 tablet/10 mL Roche complete mini EDTA-free protease inhibitor) ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently resuspended in 1 mL of lysis buffer (0.5% NP40, 50 mM Tris–HCl pH 7.4; 150 mM NaCl, 1 mM EDTA, cOmplete protease (Roche) and PhosSTOP phosphatase (Roche ...
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bioRxiv - Developmental Biology 2023Quote: Dissected embryonic brain structures were lysed in RIPA buffer (Tris HCl 1M, pH 7.7; NaCl 5M; EDTA 0.5M; Triton X-100) supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 10 µM Olaparib-containing medium for the 48 h before being washed with PBS and pre-extracted with pre-extraction buffer (10 mM Tris-HCl, 2.5 mM MgCl2, 0.5% NP-40, 100× Protease inhibitor cocktail (Roche)) for 5 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... after which they were resuspended in 1.0 ml of ice-cold lysis buffer (10 mM Tris-HCl, pH 7.7; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; protease inhibitors [Roche]) and incubated on ice for 10 min before nuclei were pelleted (400 × g ...
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bioRxiv - Genetics 2023Quote: ... The cell pellet was dried overnight and then lysed with glass beads in lysis buffer (Tris-HCl [pH 7.5], 1 mM EDTA, 2.75 mM DTT, protease inhibitor cocktail (cOmplete EDTA-free [Roche]). Next 3x SDS sample buffer (187.5 mM Tris [pH 6.8] ...
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bioRxiv - Microbiology 2023Quote: ... Nuclear pellets were resuspended in shearing buffer (0.1% SDS, 1 mM EDTA, 10 mM Tris-HCl pH 7.5, 1X Roche protease inhibitor ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted by centrifugation and resuspended in lysis buffer (40 mM Tris-HCl pH 8.0, 0.3 M NaCl, cOmplete protease inhibitor (Roche), 5 μg/mL DNase I ...
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bioRxiv - Neuroscience 2022Quote: ... For cellular extracts 50 μg of total protein isolated in NP-40 buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.6, 0.5% NP-40, protease inhibitors (Roche)) was resolved in 10% SDS-PAGE gels and then transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... 50mM TRIS-HCl pH 7.4) supplemented with protease and PhosSTOP phosphatase inhibitor (ref. 04 906 845 001 – Roche). Antibodies were diluted at 1:1000 in PBS - 0.1% Tween - 5% non-fat dried milk.
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bioRxiv - Developmental Biology 2022Quote: ... Sections were re-fixed in 4% PFA for 5 min and incubated in 0.2 M HCl for 10 min at RT before treatment with tri-ethanolamine buffer (662.5 µl tri-ethanolamine, 112 µl 1 M HCl, 49.1 µl H2ODT, 125 µl acetic anhydride; Roche). Slides were preincubated at 65°C in hybridization buffer (50% formamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells where lysed in immunoprecipitation buffer (50 mM Tris-HCl, 0.5% NP-40, and Roche protease inhibitor cocktail) and subjected to GFP trap (ChromoTek) ...
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bioRxiv - Genomics 2022Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
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bioRxiv - Plant Biology 2022Quote: ... incubated with proteinase K (1 mg/mL in 100 mM Tris-HCl, pH 8.0, 50 mM EDTA; Roche) at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 8.0, 5 mM MgCl2, 0.25% IGEPAL CA-630, 1 mM DTT, cOmplete protease inhibitor [Roche]) and mixed with 200 µL of 3 µg µL−1 nuclear extract and 500 µL PBB+ buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 8.0, 0.25% IGEPAL CA-630, 1 mM DTT, 5 mM MgCl2, cOmplete protease inhibitor by Roche). Per replicate and construct ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... 50 mM Tris-HCl pH8.0, 0.3 M NaCl, Triton X-100 1%, EDTA-free Complete Proteases inhibitor cocktail from Roche and 250U/ml of benzonase endonuclease from Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with glass beads in 250 µl RIPA buffer (25mM Tris/HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Roche complete protease inhibitor cocktail ...